beta-III Tubulin Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody [NB100-1612] - Left panel: B-III Tubulin (Green); GFAP (Red); DAPI nuclear stain (Blue).
Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody [NB100-1612] - Dissociated cell culture of a neonatal mouse brain showing beta-III Tubulin (green staining) of neurons. These cultures were ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-WhMt
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated
Concentration
0.3 mg/ml

Order Details

beta-III Tubulin Antibody Summary

Immunogen
Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the beta-III Tubulin gene product.
Marker
Neuronal Marker
Specificity
beta-III Tubulin - Dendrite Marker
Clonality
Polyclonal
Host
Chicken
Gene
TUBB3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:5000
  • Immunocytochemistry/Immunofluorescence 1:1000-1:2000
  • Immunohistochemistry 1:1000-1:2000
  • Immunohistochemistry Whole-Mount
Application Notes
Although not tested, this antibody may be useful for immonohistochemistry on frozen and paraffin sections. Use in Immunohistochemistry- Whole mount reported in scientific literature (PMID 27178445).
Reviewed Applications
Read 1 Review rated 5
using
NB100-1612 in the following applications:

Publications
Read Publications using
NB100-1612 in the following applications:

Reactivity Notes

The synthetic peptides used as an immunogen are shared between human and rat protein sequences.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
10mM Sodium PBS (0.9% isotonic, w/v, pH 7.2)
Preservative
0.02% Sodium Azide
Concentration
0.3 mg/ml
Purity
Immunogen affinity purified

Alternate Names for beta-III Tubulin Antibody

  • beta-4
  • betaIII Tubulin
  • beta-III Tubulin
  • beta-Tubulin III
  • TUBB4 CFEOM3A
  • Tubulin Beta 3
  • tubulin beta-3 chain
  • Tubulin beta-4 chain
  • Tubulin beta-4
  • Tubulin beta-III
  • tubulin, beta 3
  • TUJ1 antigen
  • TUJ-1 antigen

Background

Beta-III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of beta III is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells. In adult tissues, the distribution of beta III tubulin is almost exclusively neuron-specific.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for beta-III Tubulin Antibody (NB100-1612)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-WhMt, WB.


Filter By Application
ICC/IF
(4)
IHC
(1)
IHC-WhMt
(1)
WB
(1)
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Human
(1)
Mouse
(5)
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Review for beta-III Tubulin Antibody (NB100-1612) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.
We have 1 review tested in 1 application: ICC.

Reviews using NB100-1612:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry beta-III Tubulin NB100-1612
Enlarge
reviewed by:
Paula Loos
ICC Rat 05/19/2014
View

Summary

ApplicationImmunocytochemistry
SpeciesRat

Product General Protocols

View specific protocols for beta-III Tubulin Antibody (NB100-1612): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta-III Tubulin Antibody (NB100-1612). (Showing 1 - 2 of 2 FAQs).

  1. I would like to find out the isotype information for Catalog Number NB100-1612. Is it IgG or IgY? What kind of secondary antibody should I use?
    • Our tubulin beta 3 antibody with catalogue number NB100-1612 is a chicken polyclonal, of the isotype IgY. You can see our relevant secondary antibodies for the detection of chicken IgY at the following link: View Secondary Antibodies
  2. Do you know if the beta-III tubulin antibody (Cat NB100-1612) has been successfully used for ELISA?
    • Our tubulin beta 3 antibody with catalogue number NB100-1612 has not yet been validated for ELISA.

Secondary Antibodies

 

Isotype Controls

Additional beta-III Tubulin Products

Bioinformatics Tool for beta-III Tubulin Antibody (NB100-1612)

Discover related pathways, diseases and genes to beta-III Tubulin Antibody (NB100-1612). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for beta-III Tubulin Antibody (NB100-1612)

Discover more about diseases related to beta-III Tubulin Antibody (NB100-1612).
 

Pathways for beta-III Tubulin Antibody (NB100-1612)

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PTMs for beta-III Tubulin Antibody (NB100-1612)

Learn more about PTMs related to beta-III Tubulin Antibody (NB100-1612).

Blogs on beta-III Tubulin.

Beta Tubulin III and neurogenesis
Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.

Beta III tubulin
The Beta III tubulin protein is abundantly present in both the central and peripheral nervous systems (CNS and PNS), where it is predominantly expressed during fetal and postnatal development. In cerebellar and sympathoadrenal neurogenesis, Beta III d...  Read full blog post.

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Recent Reviews

5
5
1
4
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1
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Paula Loos
05/19/2014
Application: ICC
Species: Rat

Bioinformatics

Gene Symbol TUBB3
Entrez
Uniprot