beta-III Tubulin Antibody

Images

 
Western Blot: beta-III Tubulin Antibody [NB100-1612] - The levels of glial fibrillary acidic protein (GFAP) and S100beta as astrocytic protein marker proteins in the hippocampal area. Western blot images using GFAP, ...read more
Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody [NB100-1612] - Beta-III Tubulin in rat primary motor neurons. Ab dilution 1:1000 in PBST (0.1% Triton X-100) + 10% GS O/N at 4C. ICC/IF image submitted ...read more
Western Blot: beta-III Tubulin Antibody [NB100-1612] - The levels of glial fibrillary acidic protein (GFAP) and S100beta as astrocytic protein marker proteins in the hippocampal area. Western blot images using S100beta, ...read more
beta-III Tubulin Western Blot using homogenates of adult mouse brain (1:3000).
Immunohistochemical staining of HIF-1 alpha , VEGF-A and VEGF-C in normal renal tissue (A-C) and clear cell renal cell carcinoma (CCRCC) (D-F). A homogeneous cytoplasmic staining of tubular cells and weak staining in ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC, KD
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated
Concentration
0.3 mg/ml

Order Details

beta-III Tubulin Antibody Summary

Immunogen
Chickens were immunized with three synthetic peptide/keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of beta-III Tubulin, but are shared between the human (NP_AAL28094, NCBI) and rat (AAM28438, NCBI) protein sequences.
Marker
Neuron Cell Marker
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Gene
TUBB3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:2000-1:5000
  • Immunohistochemistry Whole-Mount
  • Immunohistochemistry 1:2000 - 1:5000
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Western Blot 1:10000 - 1:20000
Application Notes
Although not tested, this antibody may be useful for immunohistochemistry on frozen sections. Use in KD reported in scientific literature (PMID:33333046). Each of the three antibodies were analyzed by immunohistochemistry (1:2000). using fluorescein-labeled goat anti-chicken IgY (1:500). as the secondary reagent. Use in Immunohistochemistry-Whole mount reported in scientific literature (PMID 27178445). Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID: 27178445). Use in Flow Cytometry reported in scientific literature (PMID: 31755958).
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB100-1612 in the following applications:

Publications
Read Publications using
NB100-1612 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
10mM PBS (0.9% isotonic, w/v, pH 7.2)
Preservative
0.02% Sodium Azide
Concentration
0.3 mg/ml
Purity
Immunogen affinity purified

Notes

Chicken products cannot be exported to Canada.

Purification Notes
After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 300 ug/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Storage Notes
Store at 4C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

Alternate Names for beta-III Tubulin Antibody

  • Beta3-tubulin
  • beta-4
  • betaIII Tubulin
  • beta-III Tubulin
  • beta-Tubulin III
  • Class III beta-tubulin
  • TUBB3
  • TUBB4 CFEOM3A
  • Tubulin Beta 3
  • tubulin beta-3 chain
  • Tubulin beta-4 chain
  • Tubulin beta-4
  • Tubulin beta-III
  • tubulin, beta 3
  • TUJ1 antigen
  • TUJ-1 antigen

Background

Beta-III Tubulin is abundant in the central and peripheral nervous systems where it is prominently expressed during fetal and postnatal development. Beta III Tubulin is often referred to as TUJ1 because Tuj-1 is the clone name of the original monoclonal antibody. The Tuj1 clone is such a popular neuronal marker that Tuj-1 is now used as a synonym for beta III tubulin. The distribution of Tubulin Beta 3 is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of the Tubulin Beta 3 protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells. Mutation on beta-tubulin has been linked to anti-cancer drug resistance in lung cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-1612
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC, KD

Publications for beta-III Tubulin Antibody (NB100-1612)(39)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 10 applications: B/N, EM, FLOW, ICC/IF, IF/IHC, IHC, IHC-WhMt, In Vivo, KD, WB.


Filter By Application
B/N
(1)
EM
(1)
FLOW
(1)
ICC/IF
(17)
IF/IHC
(4)
IHC
(1)
IHC-WhMt
(1)
In Vivo
(1)
KD
(1)
WB
(6)
All Applications
Filter By Species
Human
(12)
Mouse
(10)
Rat
(3)
All Species
Showing Publications 1 - 10 of 39. Show All 39 Publications.
Publications using NB100-1612 Applications Species
Samal P, Kumar Samal JR, Rho HS et al. Direct deep UV lithography to micropattern PMMA for stem cell culture Materials Today Bio 2023-08-29 [PMID: 37701129]
Kumar V, Kingsley D, Madhurakkat Perikamana S et al. Self-assembled innervated vasculature-on-a-chip to study nociception Biofabrication 2023-04-13 [PMID: 36996841] (WB) WB
Xiao Y, Hu M, Lin Q et al. Dopey2 and Pcdh7 orchestrate the development of embryonic neural stem cells/ progenitors in zebrafish iScience 2023-02-01 [PMID: 36936789]
Giovanni S, serger e, Chadwick J et al. The intermittent fasting-dependent gut microbial metabolite indole-3 propionate promotes nerve regeneration and recovery after injury Research Square 2020-12-16 (IF/IHC, Mouse) IF/IHC Mouse
Watts M, Giadone R, Ordureau A et al. Analyzing ER stress response in ALS patient derived motor neurons identifies druggable neuroprotective targets bioRxiv 2023-11-17 [PMID: 38314348] (ICC/IF, Human) ICC/IF Human
Beatriz M, Rodrigues RJ, Vila�a R et al. Extracellular vesicles improve GABAergic transmission in Huntington's disease iPSC-derived neurons Theranostics 2023-06-26 [PMID: 37441602]
Fortuna TR, Kour S, Chimata AV et al. SMN regulates GEMIN5 expression and acts as a modifier of GEMIN5-mediated neurodegeneration Acta neuropathologica 2023-06-27 [PMID: 37369805]
Caparaso SM, Redwine AL, Wachs RA Engineering a multicompartment in vitro model for dorsal root ganglia phenotypic assessment Journal of biomedical materials research. Part B, Applied biomaterials 2023-06-16 [PMID: 37326300] (ICC/IF, Rat)

Details:
1:500 dilution
ICC/IF Rat
Buttermore ED, Anderson NC, Chen PF et al. 16p13.11 deletion variants associated with neuropsychiatric disorders cause morphological and synaptic changes in induced pluripotent stem cell-derived neurons Frontiers in psychiatry 2022-11-03 [PMID: 36405918]
Wong JJY, Varga BV, K�rad�ttir RT, Hall EAH. Electrochemically induced in vitro focal hypoxia in human neurons Frontiers in Cell and Developmental Biology 2022-09-28 [PMID: 36247014] (EM, B/N) EM, B/N
Show All 39 Publications.

Reviews for beta-III Tubulin Antibody (NB100-1612) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Rat.

Reviews using NB100-1612:
Filter by Applications
ICC
(2)
All Applications
Filter by Species
Rat
(2)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry beta-III Tubulin NB100-1612
Enlarge
4
reviewed by:
Verified Customer
ICC Rat 02/19/2020
View

Summary

ApplicationImmunocytochemistry
Sample TestedRat Primary Motor Neurons
SpeciesRat
LotTUJ884798

Comments

CommentsFixed in PFA 4% + Sacharose 4% in PBS 20 min RT. Ab dilution 1:1000 in PBST (0.1% triton) + 10% GS O/N 4ºC.
Immunocytochemistry beta-III Tubulin NB100-1612
Enlarge
5
reviewed by:
Paula Loos
ICC Rat 05/19/2014
View

Summary

ApplicationImmunocytochemistry
SpeciesRat

Product General Protocols

View specific protocols for beta-III Tubulin Antibody (NB100-1612): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta-III Tubulin Antibody (NB100-1612). (Showing 1 - 2 of 2 FAQs).

  1. I would like to find out the isotype information for Catalog Number NB100-1612. Is it IgG or IgY? What kind of secondary antibody should I use?
    • Our tubulin beta 3 antibody with catalogue number NB100-1612 is a chicken polyclonal, of the isotype IgY. You can see our relevant secondary antibodies for the detection of chicken IgY at the following link: View Secondary Antibodies
  2. Do you know if the beta-III tubulin antibody (Cat NB100-1612) has been successfully used for ELISA?
    • Our tubulin beta 3 antibody with catalogue number NB100-1612 has not yet been validated for ELISA.

Secondary Antibodies

 

Isotype Controls

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Blogs on beta-III Tubulin.

Successful Transplantation of Friedreich Ataxia Induced Pluripotent Stem Cell (iPSC)-Derived Sensory Neurons in Dorsal Root Ganglia of Adult Rodents
Jamshed Arslan, Pharm D, PhD The dorsal root ganglia (DRG) are a collection of cell bodies of sensory nerves carrying sensory information – including nociception, mechanoreception and proprioception – from periphera...  Read full blog post.

Beta Tubulin III and neurogenesis
Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.

Beta III tubulin
The Beta III tubulin protein is abundantly present in both the central and peripheral nervous systems (CNS and PNS), where it is predominantly expressed during fetal and postnatal development. In cerebellar and sympathoadrenal neurogenesis, Beta III d...  Read full blog post.

mFluor Violet Conjugated Antibodies

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Verified Customer
02/19/2020
Application: ICC
Species: Rat

Paula Loos
05/19/2014
Application: ICC
Species: Rat

Bioinformatics

Gene Symbol TUBB3
Entrez
Uniprot