| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22114SS | |||
| Azide and BSA Free | NBP2-80827 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65541SS | |||
| Azide and BSA Free | NBP2-80849 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-985-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-71663SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64895SS | |||
| Azide and BSA Free | NBP2-80651 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-77758-0.5mg | |||
| BSA Free | NB100-77758SS | |||
| Azide and BSA Free | NBP2-80627 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-19775SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56536SS | |||
| Azide and BSA Free | NBP2-37704 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36561SS | |||
| Azide and BSA Free | NBP2-80818 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76985-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-724SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56563SS | |||
![Western Blot: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Western blot analysis using TGF beta 1 mAb against human LAP (TGF-beta 1) recombinant protein. (Expected MW is 41 kDa)](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-7F6-Western-Blot-NBP2-22114-img0002.jpg "Western Blot: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Western blot analysis using TGF beta 1 mAb against human LAP (TGF-beta 1) recombinant protein. (Expected MW is 41 kDa)")
![Immunohistochemistry-Paraffin: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Immunohistochemical analysis of paraffin-embedded lymphoid tissue tissues using LAP (TGF-beta 1) mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-7F6-Immunohistochemistry-Paraffin-NBP2-22114-img0005.jpg)
![Flow Cytometry: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Flow cytometric analysis of A549 cells using LAP (TGF-beta 1) mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-7F6-Flow-Cytometry-NBP2-22114-img0003.jpg)
![Western Blot: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Western blot analysis of human stomach tissue (A) and human small intestine tissue (B) using TGF beta 1 antibody (NBP2-22114) at 2 ug/ml.](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-7F6-Western-Blot-NBP2-22114-img0007.jpg)
![Immunohistochemistry-Paraffin: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Human tonsil. Image from verified customer review.](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-7F6-Immunohistochemistry-Paraffin-NBP2-22114-img0006.jpg)
![Immunohistochemistry-Paraffin: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using LAP (TGF-beta 1) mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-7F6-Immunohistochemistry-Paraffin-NBP2-22114-img0004.jpg)
![ELISA: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-7F6-ELISA-NBP2-22114-img0001.jpg)
![Immunohistochemistry: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Administration of cord blood cells with plasma in the 6-OHDA PD rat model: Assessment of immune cell activation, and pro-inflammatory cytokine production in the intestine. Confocal imaging shows a significant reduction in OX-6 (MHC II) expression in the intestinal mucosa of 6-OHDA animals that were given a combination of cord blood cells and plasma. Representative merged images above show co-localization of OX-6 (Green) with DAPI+ (Blue) expression from cells in the small intestine of 6-OHDA PD animals. Arrow heads indicate positive staining of OX-6 expression in intestinal villi. Image collected and cropped by Citeab from the following publication (A Gutsy Move for Cell-Based Regenerative Medicine in Parkinson's Disease: Targeting the Gut Microbiome to Sequester Inflammation and Neurotoxicity. Stem Cell Rev Rep (2019) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MHC-Class-II-RT1B-Antibody-OX-6-Immunohistochemistry-NB100-65541-img0017.jpg "Immunohistochemistry: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Administration of cord blood cells with plasma in the 6-OHDA PD rat model: Assessment of immune cell activation, and pro-inflammatory cytokine production in the intestine. Confocal imaging shows a significant reduction in OX-6 (MHC II) expression in the intestinal mucosa of 6-OHDA animals that were given a combination of cord blood cells and plasma. Representative merged images above show co-localization of OX-6 (Green) with DAPI+ (Blue) expression from cells in the small intestine of 6-OHDA PD animals. Arrow heads indicate positive staining of OX-6 expression in intestinal villi. Image collected and cropped by Citeab from the following publication (A Gutsy Move for Cell-Based Regenerative Medicine in Parkinson's Disease: Targeting the Gut Microbiome to Sequester Inflammation and Neurotoxicity. Stem Cell Rev Rep (2019) licensed under a CC-BY license.")
![Western Blot: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Total protein from mouse placenta and spleen was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RT1b in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/MHC-Class-II-RT1B-Antibody-OX-6-Western-Blot-NB100-65541-img0011.jpg)
![Immunohistochemistry-Paraffin: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse spleen using MHC Class II RT1B antibody (clone OX-6) at 1:100 dilution. The signal was developed using HRP-labelled secondary antibody and DAB reagent, and the nuclei were counterstained with hematoxylin. The antibody generated very specific signal in a subset of spleenocytes.](http://images.novusbio.com/fullsize/MHC-Class-II-RT1B-Antibody-OX-6-Immunohistochemistry-Paraffin-NB100-65541-img0014.jpg)
![Flow Cytometry: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Staining of rat spleen cells.](http://images.novusbio.com/fullsize/MHC-Class-II-RT1B-Antibody-OX-6-Flow-Cytometry-NB100-65541-img0005.jpg)
![Immunohistochemistry: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Analysis of fluorescent intensity revealed significant protection and retention of dopaminergic cells in the SNpc in 6-OHDA animals treated with cord blood cells and plasma, compared to vehicle controls. Cord blood treated groups also demonstrated significant reductions immune cell activation (OX-6). Overall, the combination of cord blood cells and plasma can significantly modulate the exacerbated immune response through downregulation of pro-inflammatory cytokine production and immune cell activation in the 6-OHDA rat model at all time points tested. Image collected and cropped by Citeab from the following publication (A Gutsy Move for Cell-Based Regenerative Medicine in Parkinson's Disease: Targeting the Gut Microbiome to Sequester Inflammation and Neurotoxicity. Stem Cell Rev Rep (2019) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MHC-Class-II-RT1B-Antibody-OX-6-Immunohistochemistry-NB100-65541-img0019.jpg)
![Flow Cytometry: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Analysis using Alexa Fluor (R) 488 conjugate of NB100-65541. Rat Splenocytes were stained with MHC Class II RT1B (OX-6) antibody NB100-65541 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. The antibodies were directly conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/MHC-Class-II-RT1B-Antibody-OX-6-Flow-Cytometry-NB100-65541-img0012.jpg)
![Western Blot: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Rat Spleen Tissue lysate probed with MHC Class II antibody.](http://images.novusbio.com/fullsize/MHC-Class-II-RT1B-Antibody-OX-6-Western-Blot-NB100-65541-img0002.jpg)
![Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541] - Staining of rat splenocytes with Mouse anti Rat MHC class II RT1B](http://images.novusbio.com/fullsize/nb100-65541_mouse-monoclonal-mhc-class-ii-rt1b-antibody-ox-6-17720241044625.jpg)
![Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541] - Staining of rat spleen cells.](http://images.novusbio.com/fullsize/nb100-65541_mouse-monoclonal-mhc-class-ii-rt1b-antibody-ox-6-17720241044643.jpg)
![Immunocytochemistry/ Immunofluorescence: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541] - Administration of cord blood cells with plasma in the 6-OHDA PD rat model: Assessment of immune cell activation, & pro-inflammatory cytokine production in the intestine. Confocal imaging shows a significant reduction in OX-6 (MHC II) expression in the intestinal mucosa of 6-OHDA animals that were given a combination of cord blood cells & plasma. This therapy also resulted in a significant decrease in TNF expression in this animal model. Quantitative analyses of the estimated OX-6 expression & TNF are displayed in the graphs above. Representative merged images above show co-localization of OX-6 (Green) or TNF (Red) with DAPI+ (Blue) expression from cells in the small intestine of 6-OHDA PD animals. Arrow heads indicate positive staining of OX-6 & TNF expression in intestinal villi. Scale bar = 100 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31317505), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-65541_mouse-monoclonal-mhc-class-ii-rt1b-antibody-ox-6-310202415532025.jpg)
![Immunocytochemistry/ Immunofluorescence: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541] - Administration of cord blood cells with plasma in the 6-OHDA PD rat model: Assessment of TH-positive dopaminergic neurons, immune cell activation, & pro-inflammatory cytokine production. Tyrosine hydroxylase (TH) staining (top row) was used to investigate the potential effect of combined cord blood cell with plasma injection on dopaminergic neuron populations in the substantia nigra pars compacta (SNpc). Analysis of fluorescent intensity revealed significant protection & retention of dopaminergic cells in the SNpc in 6-OHDA animals treated with cord blood cells & plasma, compared to vehicle controls. Cord blood treated groups also demonstrated significant reductions immune cell activation (OX-6) & pro-inflammatory cytokine production (TNF). Overall, the combination of cord blood cells & plasma can significantly modulate the exacerbated immune response through downregulation of pro-inflammatory cytokine production & immune cell activation in the 6-OHDA rat model at all time points tested. Photomicrographs correspond to representative SN in coronal sections immune-labeled with TH, OX-6 or TNF antibody. Scale bar = 50 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31317505), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-65541_mouse-monoclonal-mhc-class-ii-rt1b-antibody-ox-6-310202415523917.jpg)
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (ED1) [NB600-985] - Cynomolgus monkey spleen. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-ED1-Immunohistochemistry-Paraffin-NB600-985-img0012.jpg "Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (ED1) [NB600-985] - Cynomolgus monkey spleen. IHC-P image submitted by a verified customer review.")
![Flow Cytometry: CD68/SR-D1 Antibody (ED1) [NB600-985] - Staining of rat peritoneal macrophages cells.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-ED1-Flow-Cytometry-NB600-985-img0008.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (ED1) [NB600-985] - Staining of rat peritoneal macrophages.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-ED1-Flow-Cytometry-NB600-985-img0009.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (ED1) [NB600-985] - Staining of rat peritoneal macrophages](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-ED1-Flow-Cytometry-NB600-985-img0010.jpg)
![Immunohistochemistry-Frozen: CD68/SR-D1 Antibody (ED1) [NB600-985] - The feline macrophage containing section was incubated with the antibody at 1:50 and the section was further stained with Alexa secondary antibody. Image was captured with an epifluorescence microscope. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-ED1-Immunohistochemistry-Frozen-NB600-985-img0015.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (ED1) [NB600-985] - Decidual Macrophages were analyzed with a BD LSRFortessa. Antibody was diluted 1:100 in staining buffer before use. Isotype control is in Red. Sample with NB600-985AF700 is in Blue. Image from the Alexa Fluor 700 version of this antibody.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-ED1-Flow-Cytometry-NB600-985-img0016.jpg)
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (ED1) [NB600-985] - Staining of rat liver](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-ED1-Immunohistochemistry-Paraffin-NB600-985-img0011.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (ED1) [NB600-985] - Staining of rat peritoneal macrophages cells.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-ED1-Flow-Cytometry-NB600-985-img0007.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (ED1) [NB600-985] - An intracellular stain was performed on Jurkat cells with BAK antibody (8B4) NBP1-74026PE (blue) and a matched isotype control NB600-985PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. Image using the PE form of this antibody.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-ED1-Flow-Cytometry-NB600-985-img0013.jpg)
![Flow (Intracellular): CD68/SR-D1 Antibody (ED1) [NB600-985] - An intracellular stain was performed on U-937 cells with CD68/SR-D1 Antibody (ED1) NB600-985PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-ED1-Flow-Intracellular-NB600-985-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody [NBP1-71663] - Staining of mouse glioma tissue using CD31/PECAM-1 Antibody (NBP1-71663) at a dilution of 1:2500. Image provided by Dr. Eric Woolf of Dignity Health.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-71663-img0009.jpg "Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody [NBP1-71663] - Staining of mouse glioma tissue using CD31/PECAM-1 Antibody (NBP1-71663) at a dilution of 1:2500. Image provided by Dr. Eric Woolf of Dignity Health.")
![Simple Western: CD31/PECAM-1 Antibody [NBP1-71663] - Image shows a specific band for CD31/PECAM 1 in 0.5 mg/mL of HUVEC lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-Simple-Western-NBP1-71663-img0010.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry: CD31/PECAM-1 Antibody [NBP1-71663] - 5-HTR1A agonist enhances neovascularization during skin wound healing (excisional punch biopsy model). CD31/PECAM-1 endothelial cell immunohistochemical staining at day 5 (A,B) and day 7 (E,F), image scale bars represented at 50 um. Results were presented as mean +/- SEM (n = 5 images per wound). Two-way ANOVA was performed and significance levels were set at *P < 0.05, **P < 0.01. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fphar.2018.01406/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-Immunohistochemistry-NBP1-71663-img0012.jpg)
![Western Blot: CD31/PECAM-1 Antibody [NBP1-71663] - Snail and EndMT-related protein expression in lungs in orthotopic murine 4T1 breast cancer model. ag Expression of Snail (see Methods). Levels of CD31/PECAM-1 determined by western blot analysis in pooled (n = 6) samples in control and 4T1 breast cancer-bearing mice from the 1st to 5th week after 4T1 cancer cell inoculation (see Methods). Results presented as fold change vs control sample corresponding to healthy mice. Total protein after transfer was used as loading control Image collected and cropped by Citeab from the following publication (Nitric oxide deficiency and endothelial-mesenchymal transition of pulmonary endothelium in the progression of 4T1 metastatic breast cancer in mice. Breast Cancer Res (2018) licensed under a CC-BY license](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-Western-Blot-NBP1-71663-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody [NBP1-71663] - HUVEC cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with CD31/PECAM-1 Antibody at 5 ug/mL overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-71663-img0011.jpg)
![Flow Cytometry: CD31/PECAM-1 Antibody [NBP1-71663] - CD31/PECAM-1 Antibody was tested in WeHi cells with Dylight 488 (green) alongside a matched isotype control (black).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-Flow-Cytometry-NBP1-71663-img0005.jpg)
![Western Blot: CD31/PECAM-1 Antibody [NBP1-71663] - Detection of CD31 in HUVEC cell lysates.](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-Western-Blot-NBP1-71663-img0008.jpg)
![Flow Cytometry: CD45 Antibody (OX-1) [NB100-64895] - Analysis using Alexa Fluor (R) 488 conjugate of CD45 antibody (OX-1). Rat Splenocytes were stained with CD45 antibody (OX-1) [NB100-64895] (blue) and a matched isotype control [NBP2-27287] (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/CD45-Antibody-OX-1-Flow-Cytometry-NB100-64895-img0005.jpg "Flow Cytometry: CD45 Antibody (OX-1) [NB100-64895] - Analysis using Alexa Fluor (R) 488 conjugate of CD45 antibody (OX-1). Rat Splenocytes were stained with CD45 antibody (OX-1) [NB100-64895] (blue) and a matched isotype control [NBP2-27287] (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Flow Cytometry: CD45 Antibody (OX-1) [NB100-64895] - Analysis using the FITC conjugate of CD45 antibody (OX-1). Staining of rat spleen cells.](http://images.novusbio.com/fullsize/CD45-Antibody-OX-1-Flow-Cytometry-NB100-64895-img0004.jpg)
![Flow Cytometry: CD45 Antibody (OX-1) - BSA Free [NB100-64895] - Representative sequential gating during flow cytometry analysis of rat non-parenchymal liver cells (n = 4). Cells were labeled with antibodies to SE-1 (NB110-68095AF488), CD31, and CD45 (NB100-64895PE). LSECs were then identified as SE-1-Alexa488+ cells (e), and the biexponential CD45-PE/CD31-APC of events (f) were used to display and select the CD45+ CD31+ subsets of LSECs. FMOs (used for gating), and single antibody staining controls are shown in Additional file. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33246411/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD45-Antibody-OX-1-BSA-Free-Flow-Cytometry-NB100-64895-img0006.jpg)
![Flow Cytometry: CD45 Antibody (OX-1) [NB100-64895] - Staining of rat peripheral blood lymphocytes with mouse anti-rat CD45 antibody (OX-1) [NB100-64895].](http://images.novusbio.com/fullsize/CD45-Antibody-OX-1-Flow-Cytometry-NB100-64895-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD14 Antibody (M5E2) [NB100-77758] - Representative immunostaining of CD14 (red) in adult human lung tissue sample with nuclei counterstained blue. This image was submitted by customer review. Image from the Alexa Fluor 647 version of this antibody.](http://images.novusbio.com/fullsize/CD14-Antibody-M5E2-Immunocytochemistry-Immunofluorescence-NB100-77758-img0009.jpg "Immunocytochemistry/Immunofluorescence: CD14 Antibody (M5E2) [NB100-77758] - Representative immunostaining of CD14 (red) in adult human lung tissue sample with nuclei counterstained blue. This image was submitted by customer review. Image from the Alexa Fluor 647 version of this antibody.")
![Flow Cytometry: CD14 Antibody (M5E2) [NB100-77758] - A surface stain was performed on human peripheral blood monocytes with CD163 (EDHu-1) antibody NB110-40686APC and a matched isotype control NBP2-27287APC. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. A co-stain was performed with NB100-77758AF488.](http://images.novusbio.com/fullsize/CD14-Antibody-M5E2-Flow-Cytometry-NB100-77758-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CD14 Antibody (M5E2) [NB100-77758] - Human lung tissue was stained with FITC-conjugated CD14 (Green) for overnight. Image from the Alexa Fluor 488 version of this antibody.](http://images.novusbio.com/fullsize/CD14-Antibody-M5E2-Immunocytochemistry-Immunofluorescence-NB100-77758-img0008.jpg)
![Flow Cytometry: CD14 Antibody (M5E2) [NB100-77758] - Human peripheral blood monocytes stained with M5E2 APC](http://images.novusbio.com/fullsize/CD14-Antibody-M5E2-Flow-Cytometry-NB100-77758-img0001.jpg)
![Flow Cytometry: CD14 Antibody (M5E2) [NB100-77758] - Analysis using the FITC conjugate of NB100-77758. Staining of Human peripheral blood monocytes with M5E2 FITC.](http://images.novusbio.com/fullsize/CD14-Antibody-M5E2-Flow-Cytometry-NB100-77758-img0004.jpg)
![Flow Cytometry: CD14 Antibody (M5E2) [NB100-77758] - Analysis using the PE conjugate of NB100-77758. Staining of CD14 in human PBMC using anti-CD14 antibody conjugated with PE. Image from verified customer review.](http://images.novusbio.com/fullsize/CD14-Antibody-M5E2-Flow-Cytometry-NB100-77758-img0005.jpg)
![Flow Cytometry: CD14 Antibody (M5E2) [NB100-77758] - Analysis using PE conjugate of NB100-77758. A cell surface stain was performed on hPBMCs with ILT5 antibody (MM0413-9S32) NBP2-11729 (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1:200 for 20 minutes at room temperature. Both antibodies were conjugated to APC. A co-stain was performed using CD14 antibody (M5E2) NB100-77758PE.](http://images.novusbio.com/fullsize/CD14-Antibody-M5E2-Flow-Cytometry-NB100-77758-img0006.jpg)
![Dual RNAscope ISH-IHC: CD14 Antibody (M5E2) [NB100-77758] - TNFA mRNA (red) and CD14 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human malignant lymph node. ACD's Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-TNFA and CD14 antibody (M5E2) at 1:200 dilution. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin, BOND Polymer Refine Red Detection and Hematoxylin and RNAscope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue).](http://images.novusbio.com/fullsize/CD14-Antibody-M5E2-Dual-RNAscope-ISH-IHC-NB100-77758-img0010.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-19775] - Analysis of IL1 beta Antibody in extracts from HeLa cells.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Western-Blot-NBP1-19775-img0004.jpg "Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-19775] - Analysis of IL1 beta Antibody in extracts from HeLa cells.")
![Immunohistochemistry: IL-1 beta/IL-1F2 Antibody - BSA Free [NBP1-19775] - In situ expression of IL-6 in glomerular and tubulointerstitial compartments in patients with diabetic nephropathy DN (right) and control group (left). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32723296/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody---BSA-Free-Immunohistochemistry-NBP1-19775-img0006.jpg)
![Immunohistochemistry: IL-1 beta/IL-1F2 Antibody [NBP1-19775] - Staining in the cytoplasm of villi and in the crypt cells of human lung carcinoma tissue.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Immunohistochemistry-NBP1-19775-img0005.jpg)
![Immunohistochemistry-Paraffin: TLR6 Antibody (86B1153.2) [NB100-56536] - Tonsil probed with TLR6 antibody at 5 ug/ml. Novus's human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/TLR6-Antibody-86B1153-2-Immunohistochemistry-Paraffin-NB100-56536-img0007.jpg "Immunohistochemistry-Paraffin: TLR6 Antibody (86B1153.2) [NB100-56536] - Tonsil probed with TLR6 antibody at 5 ug/ml. Novus's human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.")
![Flow (Intracellular): TLR6 Antibody (86B1153.2) [NB100-56536] - Analysis using the PE conjugate of NB100-56536. Staining of TLR6 in Ramos cells using this antibody. 5 ug/10^6 cells. Shaded histogram represents Ramos cells without antibody; green represents isotype control (BD Pharmingen); red represents this antibody.](http://images.novusbio.com/fullsize/TLR6-Antibody-86B1153-2-Flow-Intracellular-NB100-56536-img0014.jpg)
![Flow Cytometry: TLR6 Antibody (86B1153.2) [NB100-56536] - Cell surface analysis of TLR6 antibody in 10^6 human monocytes using 0.5 ug of this antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody ; red represents anti-TLR6 antibody. goat anti-mouse IgG PE conjugated secondary antibody was used.](http://images.novusbio.com/fullsize/TLR6-Antibody-86B1153-2-Flow-Cytometry-NB100-56536-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - Integrin beta 1 (P4C10) antibody was tested in -20 degree MeOH fixed HeLa cells at a 1:200 dilution against DyLight 488 (green). Actin and nuclei were counterstained against Phalloidin 568 (red) and DAPI (blue), respectively.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-P4C10-Immunocytochemistry-Immunofluorescence-NBP2-36561-img0001.jpg "Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - Integrin beta 1 (P4C10) antibody was tested in -20 degree MeOH fixed HeLa cells at a 1:200 dilution against DyLight 488 (green). Actin and nuclei were counterstained against Phalloidin 568 (red) and DAPI (blue), respectively.")
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - An intracellular stain was performed on A431 cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-P4C10-Flow-Cytometry-NBP2-36561-img0005.jpg)
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561]](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-P4C10-Flow-Cytometry-NBP2-36561-img0002.jpg)
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - A surface stain was performed on HeLa cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-P4C10-Flow-Cytometry-NBP2-36561-img0003.jpg)
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - A surface stain was performed on HeLa cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-P4C10-Flow-Cytometry-NBP2-36561-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - Overnight incubation with 1:50 Integrin beta 1/CD29 primary antibody in PBS/BSA 1%, at 4ºC. Green: Integrin beta 1/CD29 Antibody (P4C10), Red: Phalloidin, Blue: DAPI. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-36561_mouse-monoclonal-integrin-beta-1-cd29-antibody-p4c10-immunocytochemistry-immunofluorescence-1462023134549..jpg)
![Western Blot: TRAILR4/TNFRSF10D/DcR2 Antibody [NBP1-76985] - Analysis of 50 ug of whole cell lysate from HeLa cells with anti-DcR2 at 1:1000 dilution.](http://images.novusbio.com/fullsize/TRAILR4-TNFRSF10D-DcR2-Antibody-Western-Blot-NBP1-76985-img0001.jpg "Western Blot: TRAILR4/TNFRSF10D/DcR2 Antibody [NBP1-76985] - Analysis of 50 ug of whole cell lysate from HeLa cells with anti-DcR2 at 1:1000 dilution.")
![Immunocytochemistry/Immunofluorescence: TRAILR4/TNFRSF10D/DcR2 Antibody [NBP1-76985] - Staining of HeLa cells using DcR2 antibody at 10ug/ml.](http://images.novusbio.com/fullsize/TRAILR4-TNFRSF10D-DcR2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76985-img0002.jpg)
![Immunohistochemistry: TRAILR4/TNFRSF10D/DcR2 Antibody - BSA Free [NBP1-76985] - Immunohistochemistry of TRAILR4/TNFRSF10D/DcR2 in mouse kidney node tissue with TRAILR4/TNFRSF10D/DcR2 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/nbp1-76985_rabbit-polyclonal-trailr4-tnfrsf10d-dcr2-antibody-27112024202292.gif)
![Immunocytochemistry/ Immunofluorescence: TRAILR4/TNFRSF10D/DcR2 Antibody - BSA Free [NBP1-76985] - Immunofluorescence of TRAILR4/TNFRSF10D/DcR2 in mouse kidney tissue withTRAILR4/TNFRSF10D/DcR2 antibody at 20 ug/ml.Green: TRAILR4/TNFRSF10D/DcR2 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76985_rabbit-polyclonal-trailr4-tnfrsf10d-dcr2-antibody-2711202419592062.gif)
![Immunocytochemistry/Immunofluorescence: CTGF/CCN2 Antibody [NB100-724] - CTGF antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-724-img0004.jpg "Immunocytochemistry/Immunofluorescence: CTGF/CCN2 Antibody [NB100-724] - CTGF antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).")
![Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody [NB100-724] - Analysis of a FFPE tissue section of human lymph node using 1:200 dilution of CTGF/CCN2 antibody (NB100-724). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Immunohistochemistry-Paraffin-NB100-724-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: CTGF/CCN2 Antibody [NB100-724] - Analysis of astrocyte reactivity, the cytoskeleton & migration rate following treatment with TGF-beta 2 & CCN2/CTGF. (A) GFAP immunoreactivity (green) is increased in murine ON astrocytes following TGF-beta 2 & CCN2/CTGF treatment. Nuclei are stained with Dapi (blue) (B) Western Blot experiments analyses of alpha -actinin protein synthesis in murine ON astrocytes after the treatment with 1 ng/ml TGF-beta 2, 50 ng/ml CCN2/CTGF & 100 ng/ml CCN2/CTGF for 24 h. Densitometric analyses of Western Blot analyses shows the significant increase in alpha -actinin protein synthesis. Mean value of untreated cells (control) was set at 1. GAPDH was used to normalize protein intensity (n = 4; 50 ng CCN2/CTGF: *p = 0.029; 100 ng CCN2/CTGF: *p = 0.015; unpaired two-tailed t-test). (C) Scratch assay analyses show a significant increased migration rate for murine ON astrocytes after the treatment with ng/ml TGF-beta 2, 50 ng/ml CCN2/CTGF & 100 ng/ml CCN2/CTGF for 12 h. Mean value of migration rate of control cells was set to 100% (control: n = 15; TGF-beta 2: n = 12, **p = 0.008; 50 ng/ml CCN2/CTGF: n = 10, *p = 0.001; 100 ng/ml CCN2/CTGF: n = 4, **p = 0.0001). Data represented as mean ± SD. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35493079), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-724_rabbit-polyclonal-ctgf-ccn2-antibody-31020241683912.jpg)
![Immunohistochemistry: CTGF/CCN2 Antibody [NB100-724] - GFAP expression & synthesis in the ON & ONH in a murine glaucoma model. (A) GFAP immunoreactivity in the glial lamina of 1-month (green, upper panel) & 2 months (green, middle & lower panel) old beta B1-CTGF1 mice compared to WT controls. Immunoreactivity of GFAP not altered in 1-month old TG & WT animals (green, upper panel). 2 month old beta B1-CTGF1 mice showed an increased GFAP immunoreactivity compared to WT control (green, middle panel). Lower panel shows a magnified detail of the middle panel. Nuclei are stained w/ Dapi (blue). (B) Schmatic illustration of the optic nerve. A depicts region in the glial lamina where cross sections obtained. ONH (unmyelinated part) & ON (myelinated part) used for molecular analysis. (C) Quantification of immunohistochemical staining of GFAP in the glial lamina is not altered in 1 month old TG & WT animals (WT: n = 5; TG: n = 4). GFAP immunoreactivity is increased in 2 month old TG animals compared to WT littermates (WT: n = 10, TG: n = 13; *p = 0.039; two-tailed t-test compared to oretical mean of 1 (normalized control)). Mean value of WT animals (control) set at 1. (D) RT-PCR analyses revealed no alteration in the Gfap mRNA expression in the ON of 1-month (WT: n = 7; TG: n = 5) & 2 month old beta B1-CTGF1 mice (WT: n = 15; TG: n = 16) compared to WT controls. In the ONH the Gfap mRNA expression is increased in the 2 month old TG compared to WT animals (WT: n = 6, TG: n = 5; *p = 0.04). The mRNA expression of Gfap is not altered in 1 month old animals (WT: n = 6, TG: n = 5). Mean value of WT animals (control) set at 1. RPL32 used to normalize mRNA expression. Data represented as mean ± SEM. PRL, Prelaminar region; PSL, Postlaminar region; ON, optic nerve; ONH, optic nerve head. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35493079), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-724_rabbit-polyclonal-ctgf-ccn2-antibody-310202415523931.jpg)
![Immunohistochemistry: CTGF/CCN2 Antibody [NB100-724] - GFAP expression & synthesis in the ON & ONH in a murine glaucoma model. (A) GFAP immunoreactivity in the glial lamina of 1-month (green, upper panel) & 2 months (green, middle & lower panel) old beta B1-CTGF1 mice compared to WT controls. Immunoreactivity of GFAP not altered in 1-month old TG & WT animals (green, upper panel). 2 month old beta B1-CTGF1 mice showed an increased GFAP immunoreactivity compared to WT control (green, middle panel). Lower panel shows a magnified detail of the middle panel. Nuclei are stained w/ Dapi (blue). (B) Schmatic illustration of the optic nerve. A depicts region in the glial lamina where cross sections obtained. ONH (unmyelinated part) & ON (myelinated part) used for molecular analysis. (C) Quantification of immunohistochemical staining of GFAP in the glial lamina is not altered in 1 month old TG & WT animals (WT: n = 5; TG: n = 4). GFAP immunoreactivity is increased in 2 month old TG animals compared to WT littermates (WT: n = 10, TG: n = 13; *p = 0.039; two-tailed t-test compared to oretical mean of 1 (normalized control)). Mean value of WT animals (control) set at 1. (D) RT-PCR analyses revealed no alteration in the Gfap mRNA expression in the ON of 1-month (WT: n = 7; TG: n = 5) & 2 month old beta B1-CTGF1 mice (WT: n = 15; TG: n = 16) compared to WT controls. In the ONH the Gfap mRNA expression is increased in the 2 month old TG compared to WT animals (WT: n = 6, TG: n = 5; *p = 0.04). The mRNA expression of Gfap is not altered in 1 month old animals (WT: n = 6, TG: n = 5). Mean value of WT animals (control) set at 1. RPL32 used to normalize mRNA expression. Data represented as mean ± SEM. PRL, Prelaminar region; PSL, Postlaminar region; ON, optic nerve; ONH, optic nerve head. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35493079), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-724_rabbit-polyclonal-ctgf-ccn2-antibody-310202415535150.jpg)
![Immunocytochemistry/ Immunofluorescence: CTGF/CCN2 Antibody [NB100-724] - Analyses of ECM components in murine ON astrocytes following treatment w/ TGF-beta 2 & CCN2/CTGF. (A) Immunocytochemical staining against fibronectin (green, upper panel) showed a markedly increase following the treatment w/ 1 ng/ml TGF-beta 2 or 50 ng/ml CCN2/CTGF. Immunocytochemical staining against tropoelastin (green, lower panel) showed a pronounced increase after treatment w/ 1 ng/ml TGF-beta 2 or 50 ng/ml CCN2/CTGF. Nuclei stained w/ Dapi (blue). (B) Real-time RT-PCR analyses shows an intense upregulation of collagen 3 alpha 1, tropoelastin & fibronectin mRNA in murine ON astrocytes after treatment w/ 1 ng/ml TGF-beta 2, 50 ng/ml CCN2/CTGF or 100 ng/ml CCN2/CTGF for 24 h compared to untreated control cells (collagen 3 alpha 1: n = 3; TGF-beta 2 ***p = 0.0000004, 50 ngCTGF **p = 0.005, 100ngCTGF **p = 0.008; fibronectin: n = 6, TGF-beta 2 *p = 0.00004, 50 ng CTGF *p = 0.016, 100 ngCTGF *p = 0.05; elastin: n = 5, TGF-beta 2 *p = 0.026, 50 ng CTGF *0.04, 100 ng CTGF *p = 0.03). mRNA expression normalized to Gnb2l & mean value of control cells set at 1. (C) Western Blot analysis show an increase in protein synthesis of collagen 3 alpha 1, elastin & fibronectin in murine ON astrocytes after treatment w/ 1 ng/ml TGF-beta 2, 50 ng/ml CCN2/CTGF or 100 ng/ml CCN2/CTGF for 24 h compared to untreated control cells (collagen 3 alpha 1: n = 3, TGF-beta 2 *p = 0.03, 100 ngCTGF **p = 0.009; fibronectin: n = 5, **p = 0.005, 50 ngCTGF **p = 0.006, 100 ng CTGF *p = 0.02; tropoelastin: n = 5, TGF-beta 2 ***p = 0.0004, 50 ngCTGF **p = 0.002, 100 ngCTGF ***p = 0.00001). GAPDH & alpha -tubulin used to normalize protein synthesis & mean value of control cells set at 1. Right panel shows representative Western Blots for all three proteins. Data represented as mean ± SD. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35493079), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-724_rabbit-polyclonal-ctgf-ccn2-antibody-310202415532092.jpg)
![Immunocytochemistry/ Immunofluorescence: CTGF/CCN2 Antibody [NB100-724] - Increasing substratum stiffness causes alterations in murine ON astrocyte reactivity, actin cytoskeleton & CCN2/CTGF level. (A) Filamentous actin labeled by phalloidin (red, upper panel) is increased when murine ON astrocytes were cultured on substrates with higher stiffness (30 kPa, 60 kPa) compared to softer control (10 kPa). Increasing substratum stiffness leads to an enhanced formation of actin stress fibers. Immunoreactivity of CCN2/CTGF (green, middle panel) is markedly increased when murine ON astrocytes were grown on substrates with higher stiffness (30, 60 kPa) compared to softer control (10 kPa). Murine ON astrocytes show an enhanced GFAP protein synthesis (green, lower panel) when cultured on 30 or 60 kPa compared to 10 kPa. Nuclei were stained with Dapi (blue). (B) CCN2/CTGF protein synthesis is significantly increased in murine ON astrocytes grown on 60 kPa compared to 10 kPa (10 kPa: n = 5; 30 kPa: n = 5; 60 kPa: n = 5; *p = 0.04; unpaired two-tailed t-test). (C) Protein synthesis of GFAP (10 kPa: n = 6; 30 kPa: n = 6, **p = 0.005; 60 kPa: n = 4, *p = 0.04; unpaired two-tailed t-test) show an enhanced reactivity of murine ON astrocytes cultured on 30 kPa or 60 kPa compared to 10 kPa control. (D) Vimentin protein synthesis is significantly increased in murine ON astrocytes cultured on 60 kPa compared to 10 kPa (10 kPa: n = 4; 30 kPa: n = 4; 60 kPa: n = 3 ***p = 0.00002). Protein synthesis was normalized to alpha -tubulin & mean value of control (10 kPa) was set at 1. Data represented as mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35493079), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-724_rabbit-polyclonal-ctgf-ccn2-antibody-31020241612698.jpg)
![Flow Cytometry: TLR1 Antibody [NB100-56563] - TLR1 expression pattern in primary BMNCs from 3 MM patients analyzed by flow cytometry. CD138-positive cells were gated from the total cell population. Staining for was compared with isotype-matched controls (NC). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR1-Antibody-Flow-Cytometry-NB100-56563-img0016.jpg "Flow Cytometry: TLR1 Antibody [NB100-56563] - TLR1 expression pattern in primary BMNCs from 3 MM patients analyzed by flow cytometry. CD138-positive cells were gated from the total cell population. Staining for was compared with isotype-matched controls (NC). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.")
![Western Blot: TLR1 Antibody [NB100-56563] - analysis of TLR1 in Ramos (A), Raw (B), and (C) TLR1 transfected cell lysate using this antibody. goat anti-rabbit HRP secondary antibody was used.](http://images.novusbio.com/fullsize/TLR1-Antibody-Western-Blot-NB100-56563-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: TLR1 Antibody [NB100-56563] - All the cell lines expressed mRNA for TLR1 (Figure 1) and displayed a strong expression of TLR1 protein (Figure 2). Cell lysates were electrophoresed and blotted to PVDF membrane, which was probed with TLR1-specific antibody. To confirm the immunoreactivity of the antibody, different positive controls were included. Beta-actin was served as loading control and was used to normalize expression levels between cells. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR1-Antibody-Western-Blot-NB100-56563-img0014.jpg)
![Flow Cytometry: TLR1 Antibody [NB100-56563] - Expression of TLR1 in Fravel, L363, OPM1, RPMI8226, XG1, and NCI-H929 as determined by flow cytometry. HCMLs were stained using an intracellular staining protocol with TLR1 antibodies followed by relevant secondary fluorescent-conjugated antibodies. Filled histograms (purple) represent the isotype controls and the open histograms (red) indicate TLR1. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR1-Antibody-Flow-Cytometry-NB100-56563-img0015.jpg)
![Immunohistochemistry-Paraffin: TLR1 Antibody [NB100-56563] - analysisof human small intestine using this antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/TLR1-Antibody-Immunohistochemistry-Paraffin-NB100-56563-img0009.jpg)
![Flow Cytometry: TLR1 Antibody [NB100-56563] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56563. Staining of TLR1 in human PBMCs (lymphocyte-gated) using this antibody at 10 ul/10^6 cells. Shaded histogram represents cells alone, green represents isotype controlwas used for this test.](http://images.novusbio.com/fullsize/TLR1-Antibody-Flow-Cytometry-NB100-56563-img0013.jpg)
![Flow Cytometry: TLR1 Antibody [NB100-56563] - Intracellular analysis of TLR1 AF647 in human PBMCs (lymphocyte-gated) using this antibody at 10 ul/10^6 cells. Shaded histogram represents cells without antibody, green represents isotype control, red represents TLR1 antibody.](http://images.novusbio.com/fullsize/TLR1-Antibody-Flow-Cytometry-NB100-56563-img0012.jpg)