CD14 Antibody (M5E2) [Alexa Fluor® 700]

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Product Details

Summary
Reactivity Hu, Po, Bv, Ca, PmSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr
Clone
M5E2
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 700

Order Details

CD14 Antibody (M5E2) [Alexa Fluor® 700] Summary

Immunogen
Human peripheral blood cells.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD14
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
Application Notes
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation, and immunofluorescence microscopy. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections.
Reviewed Applications
Read 2 Reviews rated 4
using
NB100-77758AF700 in the following applications:

Reactivity Notes

Expected to cross react with Chimpanzee, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, Cotton-topped Tamarin, Common Marmoset, Squirrel Monkey.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CD14 Antibody (M5E2) [Alexa Fluor® 700]

  • CD14 antigen
  • CD14 molecule
  • CD14
  • monocyte differentiation antigen CD14
  • Myeloid cell-specific leucine-rich glycoprotein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD14 Antibody (NB100-77758AF700) (0)

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Reviews for CD14 Antibody (NB100-77758AF700) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB100-77758AF700:
Filter by Applications
Flow
(2)
All Applications
Filter by Species
Human
(2)
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Images Ratings Applications Species Date Details
Flow Cytometry CD14 NB100-77758AF700
Enlarge
4
reviewed by:
Anonymous
Flow Human 11/18/2014
View

Summary

ApplicationFlow Cytometry
Sample TestedHuman PBMC
SpeciesHuman

Blocking

Blocking Detailsno blocking

Primary Anitbody

Dilution Ratio1/100, 25 min at 4C in 2% human serum, 0.5 mM EDTA in DPBS

Details

Detection Notesflow cytometry
Wash Description2% human serum, 0.5 mM EDTA in DPBS, washed 2x after antibody staining
Flow Cytometry CD14 NB100-77758AF700
Enlarge
4
reviewed by:
Anonymous
Flow Human 11/18/2014
View

Summary

ApplicationFlow Cytometry
Sample TestedHuman PBMC
SpeciesHuman

Blocking

Blocking Detailsno blocking

Primary Anitbody

Dilution Ratio1/100, 25 min at 4C in 2% human serum, 0.5 mM EDTA in DPBS

Details

Detection Notesflow cytometry
Wash Description2% human serum, 0.5 mM EDTA in DPBS, washed 2x after antibody staining

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for CD14 Antibody (NB100-77758AF700) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-77758AF350
Alexa Fluor 405 NB100-77758AF405
Alexa Fluor 488 NB100-77758AF488
Alexa Fluor 532 NB100-77758AF532
Alexa Fluor 594 NB100-77758AF594
Alexa Fluor 647 NB100-77758AF647
Alexa Fluor 700 NB100-77758AF700
Alexa Fluor 750 NB100-77758AF750
Allophycocyanin NB100-77758APC
Biotin NB100-77758B
DyLight 350 NB100-77758UV
DyLight 405 NB100-77758V
DyLight 488 NB100-77758G
DyLight 550 NB100-77758R
DyLight 594 NB100-77758DL594
DyLight 650 NB100-77758C
DyLight 680 NB100-77758FR
DyLight 755 NB100-77758IR
FITC NB100-77759
Janelia Fluor 549 NB100-77758JF549
Janelia Fluor 646 NB100-77758JF646
PerCP NB100-77758PCP

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Blogs on CD14.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

CD14 - TLR4 is my friend in battle against infections!
CD14 is a well-characterized cell-activating receptor for lipopolysaccharide-binding protein (LBP) and peptidoglycan. It is an important modulator for lipopolysaccharide (LPS)-dependent signaling and is a component of the multi-protein complex contain...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

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Recent Reviews

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5
0
4
2
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0
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1
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Anonymous
11/18/2014
Application: Flow
Species: Human

Anonymous
11/18/2014
Application: Flow
Species: Human

Bioinformatics

Gene Symbol CD14