IL-1 beta/IL-1F2 Antibody

Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-19775] - Analysis of IL1 beta Antibody in extracts from HeLa cells.

Immunohistochemistry: IL-1 beta/IL-1F2 Antibody [NBP1-19775] - Staining in the cytoplasm of villi and in the crypt cells of human lung carcinoma tissue.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

IL-1 beta/IL-1F2 Antibody Summary

• Immunogen: A synthetic peptide made to an internal sequence of human IL-1 beta [UniProt P01584].
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IL1B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30225853).

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 26687274)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IL-1 beta/IL-1F2 Antibody

• catabolin
• IL1 beta
• IL-1 beta
• IL-1
• IL1B
• IL-1b
• IL1-BETA
• IL-1F2
• IL1F2IL-1 beta
• interleukin 1, beta
• interleukin-1 beta
• preinterleukin 1 beta
• pro-interleukin-1-beta

Background

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE).Two forms of interleukin-1, designated IL-1alpha and IL-1beta, have been described. Although encoded by distinct genes and exhibiting roughly only 25% sequence identity, IL-1alpha and IL-1beta bind to the same receptor and seem to elicit similar biological responses. This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. In T cells, IL-1 stimulates the production of IL-2 and selectively inhibits IL-4 expression and it induces B cell proliferation, maturation and immunoglobulin synthesis. NK cells require IL-1beta for production of the anti- pathogen IFN-gamma. IL-1 has also been implicated in several pathological conditions including rheumatoid arthritis, inflammatory bowel disease and atherosclerosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 beta/IL-1F2 Antibody (NBP1-19775)(10)

Publications using NBP1-19775

• Nam JE, Jo SY, Ahn CW, Kim YS Baicalin attenuates Fibrogenic process in human renal proximal tubular cells (HK-2) exposed to diabetic Milieu Life Sci. Apr 30 2020 [PMID: 32360619] (WB, Human)
• Ren G, Zheng X, Bommarito M et al. Gasdermin D: in vivo evidence of pyroptosis in spontaneous labor at term J. Matern. Fetal. Neonatal. Med. May 6 2019 [PMID: 31006293] (IHC, Human)
• Gomez-Lopez N, Romero R, Panaitescu B et al. Inflammasome activation during spontaneous preterm labor with intra-amniotic infection or sterile intra-amniotic inflammation. Am. J. Reprod. Immunol. Sep 18 2018 [PMID: 30225853] (ICC/IF, Human)
• Coyac BR, Detzen L, Doucet P et al. Periodontal reconstruction by heparan sulfate mimetic-based matrix therapy in Porphyromonas gingivalis-infected mice Heliyon Aug 1 2018 [PMID: 30101201] (IHC, Mouse)
• Gruber S, Arnold M, Cini N et al. Radioprotective Effects of Dermatan Sulfate in a Preclinical Model of Oral Mucositis-Targeting Inflammation, Hypoxia and Junction Proteins without Stimulating Proliferation. Int J Mol Sci. Jun 6 2018 [PMID: 29882770] (IHC, Mouse)
• Kim JY, Lee H, Woo J et al. Reconstruction of pathway modification induced by nicotinamide using multi-omic network analyses in triple negative breast cancer. Sci Rep. 2017 Jun 14 [PMID: 28615672] (Human)
• Gruber S, Bozsaky E, Roitinger E et al. Early inflammatory changes in radiation-induced oral mucositis : Effect of pentoxifylline in a mouse model. Strahlenther Onkol. 2017 Jun 01 [PMID: 28258409] (IHC-P, Mouse)
• Costa GM, de Oliveira AP, Martinelli PM et al. Demyelination/remyelination and expression of interleukin-1b, substance P, nerve growth factor, and glial-derived neurotrophic factor during trigeminal neuropathic pain in rats. Neurosci. Lett. 2015 Dec 11 [PMID: 26687274] (IHC-P, Rat)
• Cicchini M, Chakrabarti R, Kongara S et al. Autophagy regulator BECN1 suppresses mammary tumorigenesis driven by WNT1 activation and following parity. Autophagy 2014 Oct 30 [PMID: 25483966] (IHC)
• Omran A, Peng J, Zhang C et al. Interleukin-1 beta and microRNA-146a in an immature rat model and children with mesial temporal lobe epilepsy Epilepsia 2012 Jul [PMID: 22708826] (WB, Rat, Human)

FAQs for IL-1 beta/IL-1F2 Antibody (NBP1-19775). (Showing 1 - 1 of 1 FAQs).

1. Our customer has worked in the clinical laboratory in hospital. She want to use NB110-57028 (Heme Oxygenase 1) and NBP1-19775 (IL-1 beta) for the first time. She want to know which tissue (ex, liver...) is usually used as a positive control for IHC-(p) for the good result. Because she has several tissues from human sample to test. And then, could you recommend items for IL-17 and TNF alpha antibody for IHC (p), human sample for her?
   • For NBP1-19775, the best positive control would be human lung carcinoma. IL1 beta is produced by activated macrophages, so any sample invaded by stimulated macrophages would be acceptable. A good positive control for NB110-57028 are spleen samples or live samples from animals that were treated with heavy metals or other compounds like endotoxin. You may use the following links to chose IL 7 and TNF alpha antibodies which will match customer's mentioned criteria: IL 7 Antibodies, TNF Antibodies.

Bioinformatics

• Gene Symbol: IL1B
• Entrez:
  • 3553(Human)
  • 16176(Mouse)
  • 24494(Rat)
• Uniprot:
  • P01584()