CD14 Antibody (M5E2) [Alexa Fluor® 488]

Immunohistochemistry-Frozen: CD14 Antibody (M5E2) [Alexa Fluor 488] [NB100-77758AF488] - Human lung tissue was stained with FITC-conjugated CD14 (Green) for overnight.

Flow Cytometry: CD14 Antibody (M5E2) [Alexa Fluor 488] [NB100-77758AF488] - A surface stain was performed on human peripheral blood monocytes with CD163 (EDHu-1) antibody NB110-40686APC and a matched isotype control NBP2-27287APC. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. A co-stain was performed with NB100-77758AF488.

• Reactivity: Hu, Po, Bv, Ca, Pm
• Applications: Flow, ICC/IF, IHC, IHC-Fr
• Clone: M5E2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

CD14 Antibody (M5E2) [Alexa Fluor® 488] Summary

• Additional Information: Clone M5E2 was used by HLDA to establish CD designation.
• Immunogen: Human peripheral blood cells.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD14
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
• Application Notes: The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation, and immunofluorescence microscopy. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections.

Reactivity Notes

Expected to cross react with Chimpanzee, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, Cotton-topped Tamarin, Common Marmoset, Squirrel Monkey.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CD14 Antibody (M5E2) [Alexa Fluor® 488]

• CD14 antigen
• CD14 molecule
• CD14
• monocyte differentiation antigen CD14
• Myeloid cell-specific leucine-rich glycoprotein

Background

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils. The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD14 Antibody (NB100-77758AF488)(1)

Publication using NB100-77758AF488

• Walksman R, Lipinkski MJ, Acampado E et al. Comparison of Acute Thrombogenicity for Metallic and Polymeric Bioabsorbable Scaffolds Magmaris Versus Absorb in a Porcine Arteriovenous Shunt Model. Circ Cardiovasc Interv. 2017-08-10 [PMID: 28801538] (ICC/IF, Porcine)

Reviews for CD14 Antibody (NB100-77758AF488) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Verified Customer Flow Mouse 01/26/2018

Summary

• Application: Flow Cytometry
• Sample Tested: Mouse spleen cells
• Species: Mouse
• Lot: CKGT01-3-120-717-AF488

Comments

• Comments: Dilution: 1:100 in FACS buffer.

reviewed by: Verified Customer IHC-Fr Human 01/03/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Adult lung
• Species: Human
• Lot: CK1013-3-120717

Bioinformatics

• Gene Symbol: CD14