CD14 Antibody (M5E2) [PE]

Images

 
Flow Cytometry: CD14 Antibody (M5E2) [PE] [NB100-77758PE] - A cell surface stain was performed on hPBMCs with ILT5 antibody (MM0413-9S32) NBP2-11729 (top image) and a matched isotype control NBP2-27287 (bottom image). ...read more
Flow Cytometry: CD14 Antibody (M5E2) [PE] [NB100-77758PE] - analysis of CD14 in human PBMC using anti-CD14 antibody conjugated with PE. Image from verified customer review.

Product Details

Summary
Product Discontinued
View other related CD14 Primary Antibodies

Order Details


    • Catalog Number
      NB100-77758PE
    • Availability
      Product Discontinued

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CD14 Antibody (M5E2) [PE] Summary

Immunogen
Human peripheral blood cells.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD14
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
Application Notes
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation, and immunofluorescence microscopy. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections.
Reviewed Applications
Read 1 Review rated 5
using
NB100-77758PE in the following application:

Reactivity Notes

Expected to cross react with Chimpanzee, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, Cotton-topped Tamarin, Common Marmoset, Squirrel Monkey.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CD14 Antibody (M5E2) [PE]

  • CD14 antigen
  • CD14 molecule
  • CD14
  • monocyte differentiation antigen CD14
  • Myeloid cell-specific leucine-rich glycoprotein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD14 Antibody (NB100-77758PE) (0)

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Review for CD14 Antibody (NB100-77758PE) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow.

Reviews using NB100-77758PE:
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Images Ratings Applications Species Date Details
Flow Cytometry CD14 NB100-77758PE
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reviewed by:
Anonymous
Flow Human 11/23/2014
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Summary

ApplicationFlow Cytometry
Sample TestedHuman PBMC
SpeciesHuman

Blocking

Blocking Detailsno blocking

Primary Anitbody

Dilution Ratio1/100, 25 min at 4C in 2% human serum, 0.5 mM EDTA in DPBS

Details

Detection Notesflow cytometry, gated on monocytes
Wash Description2% human serum, 0.5 mM EDTA in DPBS, washed 2x after antibody staining

Product General Protocols

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FAQs for CD14 Antibody (NB100-77758PE) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB100-77758APC
DyLight 350 Labeled NB100-77758UV
DyLight 405 Labeled NB100-77758V
DyLight 488 Labeled NB100-77758G
DyLight 680 Labeled NB100-77758FR
DyLight 755 Labeled NB100-77758IR
FITC Labeled NB100-77759
PerCP Labeled NB100-77758PCP

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Bioinformatics Tool for CD14 Antibody (NB100-77758PE)

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Research Areas for CD14 Antibody (NB100-77758PE)

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Blogs on CD14.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

CD14 - TLR4 is my friend in battle against infections!
CD14 is a well-characterized cell-activating receptor for lipopolysaccharide-binding protein (LBP) and peptidoglycan. It is an important modulator for lipopolysaccharide (LPS)-dependent signaling and is a component of the multi-protein complex contain...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

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Recent Reviews

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1
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Anonymous
11/23/2014
Application: Flow
Species: Human

Bioinformatics

Gene Symbol CD14
Entrez