MHC Class II RT1B Antibody (OX-6) [DyLight 488]

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Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-Fr, IHC-P
Clone
OX-6
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 488

MHC Class II RT1B Antibody (OX-6) [DyLight 488] Summary

Immunogen
Rat thymocyte membrane glycoproteins
Specificity
NB100-65541 recognizes a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. This antibody cross reacts with certain mouse strains of MHC haplotypes k and s. This antibody does not react with C57BL6 or C57BL6/j mice because they are haplotype b. Analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. The clone does not react with Rat BDIX strain. Please be aware of the possible size variance seen in western blot.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RT1-B
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
For IHC-Paraffin: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose. PLP fixation is recommended for optimal results.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for MHC Class II RT1B Antibody (OX-6) [DyLight 488]

  • DMA
  • DMB
  • DP beta 1
  • DPB 1
  • DPB1
  • DRB
  • HLA class II histocompatibility antigen DM beta chain
  • HLA DMB
  • HLA DPB1
  • HLADM
  • HLADP1B
  • Major histocompatibility complex class II DP beta 1
  • Major histocompatibility complex class II
  • MHC class II antigen DMB
  • MHC DPB1
  • RING6
  • RING7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MHC Class II RT1B Antibody (NB100-65541G) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB100-65541APC
DyLight 350 Labeled NB100-65541UV
DyLight 405 Labeled NB100-65541V
DyLight 680 Labeled NB100-65541FR
DyLight 755 Labeled NB100-65541IR
PE Labeled NB100-65541PE
PerCP Labeled NB100-65541PCP

Additional MHC Class II RT1B Products

MHC Class II RT1B NB100-65541G

Bioinformatics Tool for MHC Class II RT1B Antibody (NB100-65541G)

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Diseases for MHC Class II RT1B Antibody (NB100-65541G)

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PTMs for MHC Class II RT1B Antibody (NB100-65541G)

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Blogs on MHC Class II RT1B.

The role of MHC Class II RT1B and immune response post brain injury
The major histocompatibility complex (MHC) is responsible for binding peptide fragments arising from pathogens in order to display them on the cell surface for recognition from immune cells.  Once recognized, the foreign pathogen is typically evade...  Read full blog post.

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Bioinformatics

Gene Symbol RT1-B