Integrin beta 1/CD29 Antibody (P4C10)

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Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - Integrin beta 1 (P4C10) antibody was tested in -20 degree MeOH fixed HeLa cells at a 1:200 dilution against Dylight 488 ...read more
Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561]

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, B/N, ELISA, Flow, ICC/IF, IHC-Fr, IP
Clone
P4C10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Integrin beta 1/CD29 Antibody (P4C10) Summary

Immunogen
HT1080 cells human fibrosarcoma
Specificity
Reacts with human Integrin beta 1. Epitope is not trypsin sensitive.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ITGB1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Blocking/Neutralizing
  • ELISA
  • Flow Cytometry 1:10
  • Immunocytochemistry/Immunofluorescence 1:10-1:100
  • Immunohistochemistry-Frozen 1:10-1:100
  • Immunoprecipitation
Application Notes
For ICC/IF methanol fixation is recommended. The P4C10 antibody inhibits endodermal cell and keratinocyte attachment to collagen type 1, fibronectin and laminin; also inhibits cell-cell adhesion. B/N was validated by publication, please see publications for details on procedure. This product does contain sodium azide, so we highly recommend performing a buffer exchange or dialyzation prior to incubating with live cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Western blot reported in scientific literature (PMID 27550917).
Theoretical MW
88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Integrin beta 1/CD29 Lysate (NBP2-64663)
Publications
Read Publications using
NBP2-36561 in the following applications:

  • B/N
    3 publications
  • 1 publication
  • 4 publications
  • IP
    3 publications
  • WB
    1 publication

Reactivity Notes

Human. Mouse reactivity reported in scientific literature (PMID: 27550917).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Integrin beta 1/CD29 Antibody (P4C10)

  • CD29 antigen
  • CD29
  • Fibronectin receptor subunit beta
  • FNRBVLAB
  • GPIIA
  • Integrin beta 1
  • integrin beta-1
  • integrin VLA-4 beta subunit
  • integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12)
  • ITGB1
  • MDF2
  • MSK12
  • very late activation protein, beta polypeptide
  • VLA-4 subunit beta
  • VLA-BETA

Background

Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms complexes with at least 18 alpha and 8 beta subunits in mammals. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. Integrins can be found on the cell membrane, melanosome, cleavage furrows, and lamellipodium on a subcellular level. Expression levels in tissues are dependent on the isoform.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Integrin beta 1/CD29 Antibody (NBP2-36561)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: B/N, ELISA, FLOW, IP, WB.


Filter By Application
B/N
(3)
ELISA
(1)
FLOW
(4)
IP
(3)
WB
(1)
All Applications
Filter By Species
Human
(6)
Mouse
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP2-36561 Applications Species
Fan D, Takawale A, Shen M et al. A Disintegrin and Metalloprotease-17 Regulates Pressure Overload-Induced Myocardial Hypertrophy and Dysfunction Through Proteolytic Processing of Integrin beta1. Hypertension. Aug 22 2016 [PMID: 27550917] (WB, Mouse) WB Mouse
Beausejour M, Noel D, Thibodeau S et al. Integrin/Fak/Src-mediated regulation of cell survival and anoikis in human intestinal epithelial crypt cells: selective engagement and roles of PI3-K isoform complexes. Apoptosis. 2012 Jun [PMID: 22402981] (B/N, Human) B/N Human
Li E, Brown SL, Stupack DG et al. Integrin alpha(v)beta1 is an adenovirus coreceptor. J Virol. 2001 Jun [PMID: 11333925] (IP, FLOW, Human) IP, FLOW Human
Gronthos S, Stewart K, Graves SE et al. Integrin expression and function on human osteoblast-like cells. J Bone Miner Res. 1997 Aug [PMID: 9258748] (B/N, Human) B/N Human
Takada Y, Puzon W. Identification of a regulatory region of integrin beta 1 subunit using activating and inhibiting antibodies. J Biol Chem. 1993 Aug 15 [PMID: 7688727] (B/N, FLOW, Human) B/N, FLOW Human
Mitjans F, Sander D, Adan J et al. An anti-alpha v-integrin antibody that blocks integrin function inhibits the development of a human melanoma in nude mice. J Cell Sci. 1995 Aug [PMID: 7593323] (ELISA, FLOW, IP, Human) ELISA, FLOW, IP Human
Kovach NL, Carlos TM, Yee E, Harlan JM. A monoclonal antibody to beta 1 integrin (CD29) stimulates VLA-dependent adherence of leukocytes to human umbilical vein endothelial cells and matrix components. J Cell Biol. 1992 Jan [PMID: 1370496] (IP, FLOW, Human) IP, FLOW Human

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Integrin beta 1/CD29 Antibody (NBP2-36561). (Showing 1 - 1 of 1 FAQs).

  1. I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
    • I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 488 Labeled NBP2-36561AF488
Alexa Fluor 647 Labeled NBP2-36561AF647
Allophycocyanin Labeled NBP2-36561APC
Biotin Labeled NBP2-36561B
DyLight 350 Labeled NBP2-36561UV
DyLight 405 Labeled NBP2-36561V
DyLight 488 Labeled NBP2-36561G
DyLight 550 Labeled NBP2-36561R
DyLight 650 Labeled NBP2-36561C
DyLight 680 Labeled NBP2-36561FR
DyLight 755 Labeled NBP2-36561IR
HRP Labeled NBP2-36561H
PE Labeled NBP2-36561PE
PerCP Labeled NBP2-36561PCP

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Blogs on Integrin beta 1/CD29.

Integrin Beta 1/CD29 - a cell adhesion and cell signaling protein with diverse functions
Integrins are a large family of trasmembrane proteins involved in cell adhesion and form a link between the intracellular cyskeletal proteins and extracellular matrix proteins. Integrins exist as heterodimers consisting of alpha and beta subunits....  Read full blog post.

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Bioinformatics

Gene Symbol ITGB1
Entrez
Uniprot