Integrin beta 1/CD29 Antibody (P4C10)

Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - Integrin beta 1 (P4C10) antibody was tested in -20 degree MeOH fixed HeLa cells at a 1:200 dilution against DyLight 488 (green). Actin and nuclei were counterstained against Phalloidin 568 (red) and DAPI (blue), respectively.

Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - An intracellular stain was performed on A431 cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).

Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561]

Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - A surface stain was performed on HeLa cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - A surface stain was performed on HeLa cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IP, B/N
• Clone: P4C10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Integrin beta 1/CD29 Antibody (P4C10) Summary

• Immunogen: HT1080 cells human fibrosarcoma
• Specificity: Reacts with human Integrin beta 1. Epitope is not trypsin sensitive.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGB1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot
  • ELISA
  • Flow Cytometry 1:10
  • Immunocytochemistry/Immunofluorescence 1:10-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:100
  • Immunoprecipitation
  • Block/Neutralize
• Application Notes: For ICC/IF,methanol fixation is recommended. The P4C10 antibody inhibits endodermal cell and keratinocyte attachment to collagen type 1, fibronectin and laminin; also inhibits cell-cell adhesion. This product does contain sodium azide, so we highly recommend performing a buffer exchange or dialyzation prior to incubating with live cells. Use in Western blot reported in scientific literature (PMID: 27550917).
• Theoretical MW: 88 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Integrin beta 1/CD29 Knockout HeLa Cell Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27550917).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Integrin beta 1/CD29 Antibody (P4C10)

• CD29 antigen
• CD29
• Fibronectin receptor subunit beta
• FNRBVLAB
• GPIIA
• Integrin beta 1
• integrin beta-1
• integrin VLA-4 beta subunit
• integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12)
• ITGB1
• MDF2
• MSK12
• very late activation protein, beta polypeptide
• VLA-4 subunit beta
• VLA-BETA

Background

Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms complexes with at least 18 alpha and 8 beta subunits in mammals. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. Integrins can be found on the cell membrane, melanosome, cleavage furrows, and lamellipodium on a subcellular level. Expression levels in tissues are dependent on the isoform.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 1/CD29 Antibody (NBP2-36561)(10)

Publications using NBP2-36561

• Shen L, Ke Q, Chai J et al. PAG1 promotes the inherent radioresistance of laryngeal cancer cells via activation of STAT3 Exp. Cell Res. Jun 18 2018 [PMID: 29913153] (B/N, Human)
• Li S, Xiong N, Peng Y et al. Acidic pHe regulates cytoskeletal dynamics through conformational integrin b1 activation and promotes membrane protrusion. Biochim. Biophys. Acta Apr 23 2018 [PMID: 29698684] (B/N)
• Pinnington SJL, Marshall JF, Wheeler AP. Correlative 3D Structured Illumination Microscopy and Single-Molecule Localization Microscopy for Imaging Cancer Invasion. Methods Mol. Biol. Apr 2 2018 [PMID: 29605919] (Human)
• Fan D, Takawale A, Shen M et al. A Disintegrin and Metalloprotease-17 Regulates Pressure Overload-Induced Myocardial Hypertrophy and Dysfunction Through Proteolytic Processing of Integrin beta1. Hypertension. Aug 22 2016 [PMID: 27550917] (WB, Mouse)
• Beausejour M, Noel D, Thibodeau S et al. Integrin/Fak/Src-mediated regulation of cell survival and anoikis in human intestinal epithelial crypt cells: selective engagement and roles of PI3-K isoform complexes. Apoptosis. 2012 Jun [PMID: 22402981] (B/N, Human)
• Li E, Brown SL, Stupack DG et al. Integrin alpha(v)beta1 is an adenovirus coreceptor. J Virol. 2001 Jun [PMID: 11333925] (IP, FLOW, Human)
• Gronthos S, Stewart K, Graves SE et al. Integrin expression and function on human osteoblast-like cells. J Bone Miner Res. 1997 Aug [PMID: 9258748] (B/N, Human)
• Takada Y, Puzon W. Identification of a regulatory region of integrin beta 1 subunit using activating and inhibiting antibodies. J Biol Chem. 1993 Aug 15 [PMID: 7688727] (B/N, FLOW, Human)
• Mitjans F, Sander D, Adan J et al. An anti-alpha v-integrin antibody that blocks integrin function inhibits the development of a human melanoma in nude mice. J Cell Sci. 1995 Aug [PMID: 7593323] (ELISA, FLOW, IP, Human)
• Kovach NL, Carlos TM, Yee E, Harlan JM. A monoclonal antibody to beta 1 integrin (CD29) stimulates VLA-dependent adherence of leukocytes to human umbilical vein endothelial cells and matrix components. J Cell Biol. 1992 Jan [PMID: 1370496] (IP, FLOW, Human)

FAQs for Integrin beta 1/CD29 Antibody (NBP2-36561). (Showing 1 - 1 of 1 FAQs).

1. I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
   • I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Bioinformatics

• Gene Symbol: ITGB1
• Entrez:
  • 3688(Human)
• Uniprot:
  • P05556()