Immunology reagents: Novus Biologicals

NBP2-24729

TLR9 Antibody (26C593.2) - BSA Free

(4 Reviews)

(160 Publications)

0.1 mg

NBP2-24729

<b>Genetic Strategies Validation. </b>Western Blot: TLR9 Antibody (26C593.2) [NBP2-24729] - Expression of TLR-9 protein in HB cells before and after 48 hr of transfection. Beta-actin was used as an internal control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092748) licensed under a CC-BY license.

Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Analysis of colonic LP or peritoneal cavity (PerC) of normal mice were evaluated by flow cytometry. B cells were intracellularly stained with the anti-TLR9 antibody after the cell surface staining with anti-B220 and CD5 antibodies, and examined using flow cytometry. N = 3, performed twice. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0146191) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: TLR9 Antibody (26C593.2) [NBP2-24729] - Cbl-b is required for the endocytic transit of TLR9. Representative confocal microscopic images of splenocytes from mice with indicated genotypes. For experiments, cells were stimulated through the BCR (green) for 30 minutes then fixed and stained for TLR9 (blue) and Lamp-1 (red)(n = 3). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0089792) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: TLR9 Antibody (26C593.2) [NBP2-24729] - Monkey retina tissue. Image from verified customer review.

Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Gating strategy for the detection of TLR-7 and -9 in B cell subsets. PBMC were isolated from whole blood and stained for surface markers before cells were fixed, permeabilised and stained for TLR-9. FSC and SSC were first used to gate out debris and SSC-A and SSC-H was utilized to eliminate duplicates. Further gating was done on CD45 and CD19, to target B cells. To separate between the different B cell populations, we gated on CD27 and IgD (D), followed by TLR-9 expression on these subsets. Data from one representative patient is shown. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0120383) licensed under a CC-BY license.

Western Blot: TLR9 Antibody (26C593.2) [NBP2-24729] - Analysis of TLR9 in A) human PBMC, B) human intestine, C) mouse intestine, and D) rat intestine tissue lysates using this antibody at a dilution of 3 ug/ml.

Flow Cytometry: TLR9 Antibody (26C593.2) [NBP2-24729] - Expression of TLR9 on turbinate epithelial cells from healthy controls compared to turbinate and polyp epithelial cells from patients with CRSwNP, n = 5 (A). Intracellular staining for TLR9 (open histogram, black line) and isotype control (filled histogram) on turbinate epithelial cells from a healthy control (B), turbinate epithelial cells (C) and polyp epithelial cells from a patient (D), analysed using flow cytometry. Results are presented as mean +/- SEM, **P<0.01. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0105618) licensed under a CC-BY license.

THP-1 human acute monocytic leukemia cell line was stained with Mouse anti-TLR9 (26C593.2) Protein-G purified Monoclonal Antibody conjugated to Janelia Fluor® 549 (Catalog # NBP2-24729JF549, blue histogram) or matched control antibody (orange histogram).

Alveolar epithelial cells (AECs), fibroblasts and fibrocytes all express Toll-like receptor-9. AECs, fibroblasts, fibrocytes and whole lungs were isolated from Balb/c, mice and total RNA prepared and analyzed for TLR-9 expression. The data were normalized to the expression level of TLR-9 in dendritic cells, which was set to 1 (n = 3/group). No TLR-9 message was amplified in cells from TLR-9-/- mice. (B, C) Immunohistochemistry was performed on paraffin wax-embedded whole-lung tissue from normal Balb/c mice. (B) Control staining with secondary antibody only; (C) diffuse TLR-9 staining, with notable staining in the bronchial epithelium, at the alveolar junctions and the interstitium. Fibroblasts (E) and AECs (G) were isolated from Balb/c mice and fixed. Immunofluorescence staining for TLR-9 shows that both cell types expressed TLR-9. The control slides with secondary antibody alone (D and F) did not show any auto-fluorescence or non-specific staining. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/21810214), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Ca, Eq, Pm, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Eq, Pm, Pm

Applications:

, , , , , , , , , , ,

Applications: , , , , , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-305A

Alt Catalog# IMG-305A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-24729AF350
Alexa Fluor 405	NBP2-24729AF405
Alexa Fluor 488	NBP2-24729AF488
Alexa Fluor 532	NBP2-24729AF532
Alexa Fluor 594	NBP2-24729AF594
Alexa Fluor 647	NBP2-24729AF647
Alexa Fluor 700	NBP2-24729AF700
Alexa Fluor 750	NBP2-24729AF750
Allophycocyanin	NBP2-24729APC
Biotin	NBP2-24729B
Biotin	NBP2-24890
CoraFluor 1	NBP2-24729CL1
DyLight 350	NBP2-24729UV
DyLight 405	NBP2-24729V
DyLight 488	NBP2-24729G
DyLight 550	NBP2-24729R
DyLight 594	NBP2-24729DL594
DyLight 650	NBP2-24729C
DyLight 680	NBP2-24729FR
DyLight 755	NBP2-24729IR
FITC	NBP2-24729F
HRP	NBP2-24729H
Janelia Fluor 525	NBP2-24729JF525
Janelia Fluor 549	NBP2-24729JF549
Janelia Fluor 646	NBP2-24729JF646
Janelia Fluor 669	NBP2-24729JF669
PE	NBP2-24907
PE	NBP2-24729PE
PerCP	NBP2-24729PCP
mFluor Violet 450 SE	NBP2-24729MFV450
mFluor Violet 500 SE	NBP2-24729MFV500
mFluor Violet 610 SE	NBP2-24729MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-24863
BSA Free	NBP2-24729SS

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NB400-144

CD36 Antibody - BSA Free

(9 Reviews)

(138 Publications)

0.1 ml

NB400-144

$Immunohistochemistry: CD36 Antibody [NB400-144] - Timp4-deficiency results in defective lipid digestion and absorption. (B) Immunostaining for CD36 in small intestine (proximal region) of chow-fed and HFD-fed WT and Timp4-/- mice. Western blot (C) and mRNA (D) for CD36 in enterocyte fraction of chow-fed and HFD-fed WT and Timp4-/- mice (collected from the proximal small intestine). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-05951-4), licensed under a CC-BY license.$

Immunohistochemistry-Paraffin: CD36 Antibody [NB400-144] - Staining of CD36 in human adipocytes.

Western Blot: CD36 Antibody [NB400-144] - Mouse primary muscle cell lysate. Western blot image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: CD36 Antibody [NB400-144] - Bone marrrow derived mouse macrophages stained with CD36 antibody. ICC/IF image submitted by a verified customer review.

Western Blot: CD36 Antibody [NB400-144] - Detection of CD36 in human adipocyte extract (30 ug). Lane 1: 0.5 ug/ml NB 400-144; lane 2: 2 ug/ml NB 400-144. ECL: 3 second exposure.

ATM activation by chloroquine alleviates senescence.(a) Immunoblots showing protein levels of ATM, NBS1, and RAP80 in human skin fibroblasts (HSFs). A gradually increased level of p16 indicates cellular senescence, while elevated gamma H2AX level indicates accumulated DNA damage. (b) Immunoblots showing protein levels of ATM, NBS1, and RAP80 in mouse embryonic fibroblasts (MEFs). (c) Immunoblots showing protein levels of ATM, NBS1, and RAP80 in brain tissues isolated from 3-, 10-, and 18-month-old male mice. (d) SA-beta -Gal staining in HSFs treated with sh-ATM or scramble shRNA. Scale bar, 100 µm. (e) Quantification of SA-beta -Gal-positive staining of (d) from five views randomly captured for each group. Data represent means ± SEM. ***p<0.001. (f) Immunoblots showing increased gamma H2AX and unaffected LC3I/II in HSFs treated with sh-ATM or scramble shRNA. (g) Immunoblots showing protein levels of pS1981 ATM, gamma H2AX, and cleaved caspase-3 in HSFs treated with 10 μM of CQ for indicated time. (h) SA-beta -Gal staining in HSFs expressing either scramble or ATM shRNA treated with 1 μM CQ or DMSO (12 hr). Scale bar, 100 µm. (i) Quantification of SA-beta -Gal-positive staining of (h) from five views randomly captured for each group. Data represent means ± SEM. ***p<0.001; ‘N.S.’ indicates no significant difference. (j) HSFs at passage 20 were continuously cultured with 1 μM CQ or DMSO, and cell number was calculated at each passage. Data represent means ± SEM. ***p<0.01. (k) Immunoblots showing protein levels of gamma H2AX, p62, and LC3 in MEFs treated with 1 μM CQ or DMSO. Note that CQ had little effect on the expression levels of p62 and LC3. (l) MEFs at passage one were continuously cultured in 20% O2 with 1 μM CQ or DMSO, and cell number was determined at each passage. Data represent means ± SEM. ***p<0.01.10.7554/eLife.34836.006Figure 1—source data 1.Statistical analysis for SA-beta -Gal positive staining.10.7554/eLife.34836.007Figure 1—source data 2.Statistical analysis for EdU positive staining.Statistical analysis for SA-beta -Gal positive staining.Statistical analysis for EdU positive staining.Decline of ATM-centered DNA repair machinery during senescence.(a) Real-time PCR analysis showing progressively elevated mRNA level of p21 in continuously cultured human endothelial cells (HUVEC). **p<0.01. (b) SA-beta -Gal staining of HUVEC cells at indicated passages. Scale bar, 100 µm. (c) HUVEC cells at P21, P18, P12, and P7 were subjected to transcriptome analysis. A minimum average rpkm value of 1.0 and maximum 10% fluctuation in young cells (P7 Vs P12) was set as the threshold. Genes were downregulated by more than 20% in pre-senescent, and senescent cells compared with young cells (P21/P18 Vs P12/P7) were selected. (d) Pathway analysis of genes identified in (c) by STRING v10. (e) Downregulation of ATM-related DNA repair genes during senescence.ATM regulates replicative senescence.(a) Representative images showing cells treated with Scramble (sh-NC) or sh-ATM. (b) Percent EdU-positive cells in sh-NC or sh-ATM treated HSFs. Views were randomly captured and at least 100 cells were included in each group. Data represent means ± SEM. ***p<0.001. (c) Immunoblots showing protein levels of pS1981 ATM and gamma H2AX in HSFs treated with 10 μM chloroquine (CQ) or 0.4 μM CPT (4 hr). Note that CQ activated ATM (pS1981) without increasing gamma H2AX, while CPT activated ATM accompanied by increased gamma H2AX. (d) SA-beta -Gal staining in primary MEFs treated with 1 μM CQ or DMSO. Scale bar, 100 µm. (e) Quantification of SA-beta -Gal-positive staining of (d) from five views randomly captured for each group. Data represent means ± SEM. ***p<0.001. (f) Percent EdU-positive cells in HSFs treated with DMSO, 1 μM or 10 μM CQ. Views were randomly captured and at least 100 cells were included in each group. Data represent means ± SEM. ***p<0.001. (g) Representative images showing proliferative HSFs treated with different doses of CQ for the indicated time points. (h) Immunoblots showing LC3B levels in HSFs treated with indicated dose of CQ for indicated period of time. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29717979), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Rabbit Polyclonal CD36 Antibody [NB400-144] - The western blot was conducted on avian (passerine) skeletal muscle tissue homogenate. 6ug of total protein was loaded and GAPDH was probed to ensure total protein loads were similar across samples. Rabbit polyclonal CD36 antibody (NB400-144) was used at 1:5000 dilution (diluted in 5% milk). CD36 and GAPDH were probed sequentially - not multiplexed. CD36 produced a band above 40kDa and GAPDH at 36kDa. Image from a verified customer review.

$Immunocytochemistry/ Immunofluorescence: CD36 Antibody - BSA Free [NB400-144] - Timp4-deficiency results in defective lipid digestion & absorption. Fecal triglyceride (TG) content (Ai) in chow- or HFD-fed WT & Timp4−/− mice. Fecal lipid analysis by thin layer chromatography in chow-fed (Aii) & HFD-fed (iii) WT & Timp4−/− mice. (B) Immunostaining for CD36 in small intestine (proximal region) of chow-fed & HFD-fed WT & Timp4−/− mice. Western blot (C) & mRNA (D) levels for CD36 in enterocyte fraction of chow-fed & HFD-fed WT & Timp4−/− mice (collected from the proximal small intestine). (E) Triglyceride levels in enterocytes collected from the proximal small intestine. Averaged data are presented as mean ± S.E.M & analyzed by ANOVA. * indicates significance (p < 0.05). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-05951-4), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Western Blot: CD36 Antibody - BSA Free [NB400-144] - Timp4-deficiency results in defective lipid digestion & absorption. Fecal triglyceride (TG) content (Ai) in chow- or HFD-fed WT & Timp4−/− mice. Fecal lipid analysis by thin layer chromatography in chow-fed (Aii) & HFD-fed (iii) WT & Timp4−/− mice. (B) Immunostaining for CD36 in small intestine (proximal region) of chow-fed & HFD-fed WT & Timp4−/− mice. Western blot (C) & mRNA (D) levels for CD36 in enterocyte fraction of chow-fed & HFD-fed WT & Timp4−/− mice (collected from the proximal small intestine). (E) Triglyceride levels in enterocytes collected from the proximal small intestine. Averaged data are presented as mean ± S.E.M & analyzed by ANOVA. * indicates significance (p < 0.05). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-05951-4), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Western Blot: CD36 Antibody - BSA Free [NB400-144] - MiR-29a inhibits the expression of fatty acid translocase CD36 by targeting 3’ untranslated region (UTR). (A) qPCR analysis of Cd36 in live tissue. (B) Representative immunoblotting bands & densitometric results of CD36 in liver tissue. (C) qPCR analysis of cd36 expression of HepG2 cells in vitro after 48h transfection of scramble sequence or miR-29a-mimic. Data obtained from six independent experiments. (D) Upper panel, sequence information, & mutual pairing status of CD36-3’UTR, mmu-miR29a, & CD36-3’UTR Mut. Note that red characters represent mismatching sites. HepG2 was first transfected with CD36-3’UTR or CD36-3’UTR mutant luciferase reporter construct then treated with control medium (ctrl), miRNA-scramble, or miR-29a mimic, & finally lysed to detect the luciferase signal. Data are expressed as mean ± SE. * p < 0.05, ** p < 0.01, & *** p < 0.001 between the indicated groups. WT, wild type mice. HFD, high-fat diet. miR-29a, mice harboring overexpression of miR-29a. mmu-miR29a, mouse-origin miR-29a. ctrl, control. Mut, mutant. UTR, untranslated region. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31652636), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: CD36 Antibody - BSA Free [NB400-144] - Integrin beta 3 modulates SMC transdifferentiation. a–c Mice were fed a HFD for 6 or 16 weeks as indicated, & then transverse aortic root sections were stained. In a, b sections from ApoE(−/−), SMMHC-CreERT2, ROSA26R(mTmG/+) mice were stained for SMA, GFP (fate marker), nuclei (DAPI), & either integrin beta 3 (a) or CD36 (b). Dashed yellow lines separate cap from core (a, b) & core from media (b). n = 5. In c sections from ApoE(−/−) mice that were also wild type or null for Itgb3 were stained for SMMHC, CD68, & nuclei (DAPI). n = 3. Boxed regions (a, c) are shown as close-ups on right; in c CD68+SMMHC+ cells in the media (arrowheads) & plaque (arrows) of the Itgb3 null atherosclerotic aorta are indicated. Med, tunica media; Lu, lumen; Pl, plaque. Scale bars, 25 μm. d–h Aortic SMCs were isolated from ApoE(−/−) mice & then subjected to siRNA-mediated knockdown with si-Itgb3 vs. scrambled (Scr; d–f) or with si-Itgb3, si-Tlr4 vs. si-Itgb3 (g, h). Levels of indicated transcripts from qRT-PCR are relative to Gapdh & normalized to either Scr in d, f or to si-Itgb3 in g, h. For d, g, n = 4–5 in duplicate. In e silenced SMCs were cultured with DiI-conjugated ox-LDL for 10 h & stained with DAPI; n = 5. In f, h silenced SMCs were exposed to soluble cholesterol:methyl-beta -cyclodextrin complexes for 3 days, & then mRNA levels were assessed; n = 4–7 in duplicate. *, **, ***, ^, **** vs. control (Scr in d, f & si-Itgb3 in g, h), p < 0.05, p < 0.01, p < 0.005, p < 0.001, & p < 0.0005, respectively. NS, not significant. Student’s t-test was used, & error bars represent standard deviations. i Schematic of the effect of beta 3 reduction via TLR4 & CD36 in SMCs on transdifferentiation Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-04447-7), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: CD36 Antibody - BSA Free [NB400-144] - The increase in CD36 levels caused by lipids in Sirt3-deficient hepatocytes is mediated by Nrf2. VLDLR mRNA abundance (a) & protein levels of VLDLR & NQO1, an Nrf-2-target gene, (b) were assessed in Huh-7 cells incubated with fatty acid free-BSA or BSA-palmitate (0.3 mM) & exposed to either vehicle or the Sirt3 inhibitor AAPBO (100 μM) for 16 h. a, p < 0.05 vs. CT. b, p < 0.05 vs. CT cells incubated with palmitate. c, p < 0.05 vs. CT cells treated with AAPBO. c, fatty acid uptake in Huh-7 cells incubated with fatty acid free-BSA or BSA-palmitate (0.3 mM) & exposed to either vehicle or the Sirt3 inhibitor AAPBO (100 μM) for 16 h was measured by the uptake of BODIPY-C16. a, p < 0.05 vs. CT. b, p < 0.05 vs. CT cells incubated with palmitate. c, p < 0.05 vs. CT cells treated with AAPBO. mRNA abundance (d) & protein levels of VLDLR (e) in Huh-7 cells transfected with control (CT) or SIRT3 siRNA & incubated in the presence or absence 0.3 mM palmitate (Pal) for 24 h. Protein levels of CD36 (f), NQO1 (g) & PPAR gamma (h) in Huh-7 cells transfected with control (CT) or SIRT3 siRNA & incubated in the presence or absence 0.3 mM palmitate (Pal) or the Nrf2 inhibitor ML385 (10 μM) for 24 h. a, p < 0.05 vs. CT siRNA cells. b, p < 0.05 vs. CT siRNA cells incubated with palmitate. c, p < 0.05 vs. SIRT3 siRNA cells. d, p < 0.05 vs. CT siRNA cells incubated with palmitate & ML385 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32912335), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Av, Bv, Pm, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Av, Bv, Pm, Rb

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB400-144AF350
Alexa Fluor 405	NB400-144AF405
Alexa Fluor 488	NB400-144AF488
Alexa Fluor 532	NB400-144AF532
Alexa Fluor 594	NB400-144AF594
Alexa Fluor 647	NB400-144AF647
Alexa Fluor 700	NB400-144AF700
Alexa Fluor 750	NB400-144AF750
Allophycocyanin	NB400-144APC
Biotin	NB400-144B
CoraFluor 1	NB400-144CL1
DyLight 350	NB400-144UV
DyLight 405	NB400-144V
DyLight 488	NB400-144G
DyLight 550	NB400-144R
DyLight 594	NB400-144DL594
DyLight 650	NB400-144C
DyLight 680	NB400-144FR
DyLight 755	NB400-144IR
FITC	NB400-144F
HRP	NB400-144H
Janelia Fluor 525	NB400-144JF525
Janelia Fluor 549	NB400-144JF549
Janelia Fluor 646	NB400-144JF646
Janelia Fluor 669	NB400-144JF669
PE	NB400-144PE
PerCP	NB400-144PCP
mFluor Violet 450 SE	NB400-144MFV450
mFluor Violet 500 SE	NB400-144MFV500
mFluor Violet 610 SE	NB400-144MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB400-144SS

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NBP2-42225

CD63 Antibody (H5C6) - BSA Free

(5 Reviews)

(59 Publications)

0.1 ml

NBP2-42225

Immunocytochemistry/Immunofluorescence: CD63 Antibody (H5C6) [NBP2-42225] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-CD63 Antibody [H5C6] conjugated to DyLight 550 (NBP2-42225R) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - An intracellular stain was performed on SK-MEL-28 cells with CD63 (H5C6) Antibody NBP2-42225R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.

Immunocytochemistry/Immunofluorescence: CD63 Antibody (H5C6) [NBP2-42225] - MDCK cells stained with CD63 antibody at a dilution of 1:50 followed by Donkey anti-mouse secondary antibody conjugated with Alexa Fluor 488 (1:500). Nuclei were stained with Hoechst 33342. ICC/IF image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: CD63 Antibody (H5C6) [NBP2-42225] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-CD63 Antibody [H5C6] conjugated to Alexa Fluor 488 (NBP2-42225AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/Immunofluorescence: CD63 Antibody (H5C6) [NBP2-42225] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-CD63 Antibody [H5C6] conjugated to Biotin (NBP2-42225B) at 5 ug/ml for 60 minutes at room temperature and detected with Streptavidin Protein conjugated to DyLight 488 at a 1:500 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/Immunofluorescence: CD63 Antibody (H5C6) [NBP2-42225] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-CD63 [H5C6] conjugated to Alexa Fluor 488 [NBP2-42225AF488] at 10ug/mL for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Western Blot: CD63 Antibody (H5C6) - BSA Free [NBP2-42225] - Analysis of common EV markers, where 10 ug of total protein was loaded in each lane. cASCs-EVs expressed CD63 and CD9, while beta actin and lamin A showed lower expression. The displayed data represent at least three repeated experiments with consistent results. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32040478/) licensed under a CC-BY license.

Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - An intracellular stain was performed on HeLa cells with CD63 [H5C6] Antibody NBP2-42225AF594 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.

Western Blot: CD63 Antibody (H5C6) [NBP2-42225] - The same samples and volumes were run under reducing and non-reducing conditions. All procedures were performed in parallel for both conditions. L- ladder +- exosomes. Image visualized with HRP linked to secondary antibody. Western blot image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: CD63 Antibody (H5C6) [NBP2-42225] - HEK cells stained with CD63 antibody at a dilution of 1:50 followed by Donkey anti-mouse secondary antibody conjugated with Alexa Fluor 488 (1:500). Nuclei were stained with Hoechst 33342. ICC/IF image submitted by a verified customer review.

Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - Human peripheral blood cells were stained (2 x 10^6 cells/mL) using the anti-CD63 antibody (Blue) at a dilution of 1:1000. Signal was detected using a Gt x Ms DyLight 488 Secondary and gated to the monocyte/granulocyte cell populations. Isotype was Mouse IgG1 kappa (orange). Data collected on BD FACS Calibur flow cytometer.

Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - A431 cells were stained (1 x10^6 cells/mL) using the anti-CD63 antibody at a 1:1000 dilution (blue). Signal was detected with Gt x Ms DyLight 488 secondary. Isotype control (orange).

Flow (Intracellular): CD63 Antibody (H5C6) [NBP2-42225] - An intracellular stain was performed on HepG2 cells with CD63 Antibody (H5C6) NBP2-42225AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - An intracellular stain was performed on HeLa cells with CD63 Antibody (H5C6) NBP2-42225APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.

Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - An intracellular stain was performed on SK-MEL-28 cells with CD63 Antibody (H5C6) NBP2-42225AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - An intracellular stain was performed on HeLa cells with CD63 [H5C6] Antibody NBP2-42225AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

CD63 (H5C6) was detected in immersion fixed MCF7 human breast cancer cell line using Mouse anti-CD63 (H5C6) Protein G Purified Monoclonal Antibody conjugated to Alexa Fluor® 488 (Catalog # NBP2-42225AF488) (green) at 5 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

CD63 (H5C6) was detected in immersion fixed A431 human skin carcinoma cell line using Mouse anti-CD63 (H5C6) Protein-G purified Monoclonal Antibody conjugated to DyLight 550 (Catalog # NBP2-42225R) (red) at 5 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

An intracellular stain was performed on A431 human skin carcinoma cell line using Mouse anti- CD63 (H5C6) Protein-G purified Monoclonal Antibody conjugated to DyLight 550 (Catalog # NBP2-42225R, blue histogram) or matched control antibody (orange histogram) at 2.5 µg/mL for 30 minutes at RT.

Western Blot: CD63 Antibody (H5C6) - BSA Free [NBP2-42225] - NCAM as a neuronal exosome marker in plasma. (A–C) Quantitative analysis of plasma EVs using flow cytometry. Dot plots of fluorescent intensity for plasma EVs stained with PE-CY7-labeled CD63 antibody. EVs were unstained (A) & stained with either isotype control (B) or CD63 antibody (C). (D) A18945 iPSC-derived neurons cultured for 6 weeks & immunostaining with mature neuronal markers (DAPI, MAP2, NeuN, & merged image). Images were taken using Zeiss LSM confocal microscope at ×63 magnification. Scale bar, 20 μm. (E) Representative images of cortical organoids at day 90 of differentiation. Scale bar, 1 mm. (F) The organoids express cortical layer marker BRN2 (also called POU3F2). The nuclei were stained with DAPI. Scale bar, 100 μm. (Gi) Markers for proliferating neural progenitors (SOX2) & cortical neuron marker CTIP2 (also known as BCL11B) with nuclei DAPI staining (Gii). (H) Western blot analysis of NCAM & CD63 from EVs released from iPSCs, iPSC-derived cortical neurons, & iPSC-derived brain organoids. EVs were isolated using SEC from the cell culture media of each sample, & equal EV particle numbers (6 × 108) were subject to immunoblotting with NCAM & CD63 antibodies. (I–L) Flow cytometry dot plots of fluorescent intensity for plasma EVs double-stained with DiI & NCAM antibody. EV samples were unstained (I), single stained with DiI (J), & double-stained with DiI & NCAM antibody in the absence (K) & presence (L) of 0.1% Triton X-100. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35655952), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

CD63 (H5C6) was detected in immersion fixed U-2 OS human osteosarcoma cell line using Mouse anti- CD63 (H5C6) Protein-G purified Monoclonal Antibody conjugated to Biotin (Catalog # NBP2-42225B) at 5 µg/mL overnight at 4C. Cells were stained using Streptavidin conjugated to DyLight 550 (red) and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Reactivity:

Hu, Ca

Species Glossary

Reactivity: Hu, Ca

Applications:

, , , , , , , , ,

Applications: , , , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-42225AF350
Alexa Fluor 405	NBP2-42225AF405
Alexa Fluor 488	NBP2-42225AF488
Alexa Fluor 532	NBP2-42225AF532
Alexa Fluor 594	NBP2-42225AF594
Alexa Fluor 647	NBP2-42225AF647
Alexa Fluor 700	NBP2-42225AF700
Alexa Fluor 750	NBP2-42225AF750
Allophycocyanin	NBP2-42225APC
Allophycocyanin/Cy7	NBP2-42225APCCY7
Biotin	NBP2-42225B
CoraFluor 1	NBP2-42225CL1
DyLight 350	NBP2-42225UV
DyLight 405	NBP2-42225V
DyLight 488	NBP2-42225G
DyLight 550	NBP2-42225R
DyLight 594	NBP2-42225DL594
DyLight 650	NBP2-42225C
DyLight 680	NBP2-42225FR
DyLight 755	NBP2-42225IR
FITC	NBP2-42225F
HRP	NBP2-42225H
Janelia Fluor 525	NBP2-42225JF525
Janelia Fluor 549	NBP2-42225JF549
Janelia Fluor 646	NBP2-42225JF646
Janelia Fluor 669	NBP2-42225JF669
PE	NBP2-42225PE
PE/Atto594	NBP2-42225PEATT594
PE/Cy5.5	NBP2-42225PECY55
PE/Cy7	NBP2-42225PECY7
PerCP	NBP2-42225PCP
mFluor Violet 450 SE	NBP2-42225MFV450
mFluor Violet 500 SE	NBP2-42225MFV500
mFluor Violet 610 SE	NBP2-42225MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-42225SS
Azide and BSA Free	NBP2-80654

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NLS1878

FPRL1/FPR2 Antibody - BSA Free

(4 Reviews)

(42 Publications)

0.05 ml

NLS1878

Immunohistochemistry-Paraffin: FPRL1/FPR2 Antibody - BSA Free [NLS1878] - SPM receptors differentially expressed on endothelium and infiltrating leukocytes AE

Flow Cytometry: FPRL1/FPR2 Antibody - BSA Free [NLS1878] - Flow Cytometry: [Alexa Fluor® 700] [NLS1878AF700] - Mouse splenocytes. Cells were pre-gated with live/dead and FSC-A/W to exclude dead cells and cell doublets. Image from verified customer review. Image using the Alexa Fluor 700 form of this antibody.

<b>Biological Strategies Validation. </b>Western Blot: FPRL1/FPR2 Antibody - BSA Free [NLS1878] - Murine neutrophils were extracted from the bone marrow of C57BL/6 mice and exposed to normal glucose (90 mg/dl) or high glucose (300 mg/dl) and the expression of FPR2 was assessed by western blotting after 1, 2, or 3 hr exposure to glucose. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35112667/) licensed under a CC-BY license.

Western Blot: FPRL1/FPR2 Antibody - BSA Free [NLS1878] - Analysis in HL-60 cell lysate.

Immunocytochemistry/Immunofluorescence: FPRL1/FPR2 Antibody - BSA Free [NLS1878] - Antibody was tested in Raw264.7 cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).

Immunohistochemistry: FPRL1/FPR2 Antibody - BSA Free [NLS1878] - Analysis in human kidney cancer using DAB with hematoxylin counterstain.

Electron Microscopy: FPRL1/FPR2 Antibody - BSA Free [NLS1878] - Electron micrographs of FPR2 immunostained sections from the prefrontal cortex. Immunostaining is mostly present in axon pre-terminals (PrT) that did not form synapses with postsynaptic structures. Occasional labelled dendrites (DE) are also found, that formed asymmetrical synapses (S) with unlabelled axon terminals (AT). Scale: a, b, c=50A nm, e=100A nm. Image collected and cropped by Citeab from the following publication (Localisation of Formyl-Peptide Receptor 2 in the Rat Central Nervous System and Its Role in Axonal and Dendritic Outgrowth. Neurochem Res (2018)) licensed under a CC-BY license.

Immunohistochemistry: FPRL1/FPR2 Antibody - BSA Free [NLS1878] - Electron micrographs of FPR2 immunostained sections from the prefrontal cortex. a, b Immunostaining is mostly present in axon pre-terminals (PrT) that did not form synapses with postsynaptic structures. c Occasional axon terminals (AT) are observed to form asymmetrical, putatively glutamatergic synapses (S) with unlabelled dendrites (DE). d Occasional labelled dendrites (DE) are also found, that formed asymmetrical synapses (S) with unlabelled axon terminals (AT). Scale: a, b, c = 50 nm, e = 100 nm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29948727), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: FPRL1/FPR2 Antibody - BSA Free [NLS1878] - Lipoxin A4, along with its precursors & ligand, were altered by hAECs. (A): Expression of the lipoxin A4 precursor genes ALOX‐5, ‐12, & ‐15 was increased at days 5 & 7 compared with saline controls (ALOX‐5: 0.393 ± 0.114 vs. 3.777 ± 1.98; ALOX‐12: 0.484 ± 0.248 vs. 1.520 ± 0.203; ALOX‐15: 0.020 ± 0.007 vs. 1.998 ± 0.983). ∗, p < .05. (B): Lipoxin A4 protein levels were elevated in lung lysates at day 7 in animals treated with hAECs compared with controls (0.103 ± 0.021 ng/ml vs. 0.249 ± 0.072 ng/ml, respectively). ∗, p < .05. (C): At day 7, bleomycin challenge resulted in positively stained F4/80/FPR2 cells, which were was elevated in mice treated with hAECs compared with control mice (5.720% ± 0.587% vs. 8.795% ± 0.687%, respectively). ∗, p < .05. (D): Representative images of F4/80‐ & FPR2‐positive‐stained lung sections from hAEC‐treated animals. Magnification: ×200. Scale bar =100 μm. Abbreviations: DAPI, 4′,6‐diamidino‐2‐phenylindole; FPR2, N‐formyl peptide receptor 2; hAEC, human amnion epithelial cell. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28371562), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: FPRL1/FPR2 Antibody - BSA Free [NLS1878] - Higher expression of Fpr2 in the livers of female mice is related with hepatocyte protection.a qRT-PCP analysis for Fpr2 expression in primary hepatocytes (pHEPs) from WT (male n = 2, female n = 2) & KO mice (male n = 2, female n = 2). Total eight mice were employed in each hepatocyte isolation, & the experiments were replicated at least three times & the mean ± S.E.M. results are graphed (*p < 0.05, **p < 0.005 vs WT male-pHEPs). b Western blot analysis & c double immunofluorescent images of Fpr2 (red) with albumin (green) in these cells. Gapdh was used as internal control. DAPI (blue) was used as nuclear counterstaining. Data shown represent one of three experiments with similar results (Scale bar, 20 μm). d qRT-PCR analysis for Fpr2 & glucose-6-phosphatase (G6pc) in, e cell viability of, & f western blot analysis of cleaved Caspase-3 & pro Caspase-3 in WT & KO female mice-isolated pHEPs treated with vehicle (Veh) or 250 μM of palmitate (PA). The data shown represent one of three experiments with similar results. The mean ± S.E.M. results obtained from three repetitive experiments are graphed (*p < 0.05, **p < 0.005 vs WT-Veh). Gray circles represent individual data points. See Supplementary Data for statistical details. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35102146), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Ba

Species Glossary

Reactivity: Hu, Mu, Rt, Ba

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 405	NLS1878AF405
Alexa Fluor 488	NLS1878AF488
Alexa Fluor 647	NLS1878AF647
Alexa Fluor 700	NLS1878AF700
Biotin	NLS1878B
DyLight 405	NLS1878V
DyLight 488	NLS1878G
DyLight 550	NLS1878R
DyLight 650	NLS1878C
FITC	NLS1878F
HRP	NLS1878H

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NLS1878SS

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NBP2-22217

LAMP-2/CD107b Antibody (H4B4) - BSA Free

(2 Reviews)

(33 Publications)

0.1 ml

NBP2-22217

Western Blot: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Analysis of LAMP-2/CD107b expression in Whole Cell Lysates: 2) HeLa, 3) Jurkat, 4) U937, 5) HepG2, Tissue Extracts: 6) Human kidney, 7) Human lung, 8) Human liver, and 9) Human placenta.

Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Staining of LAMP-2/CD107b in human liver using DAB with hematoxylin counterstain.

Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - An intracellular stain was performed on U937 cells with HGF LAMP-2/CD107b [H4B4] Antibody NBP2-22217AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Simple Western: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Lane view shows a specific band for LAMP-2/CD107b in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

$Western Blot: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Localization of TMEM135 to the mitochondria. The mitochondrial fraction isolated from the WT mouse brain show TMEM135 signals by immunoblotting. Following proteins were used as organelle markers: MFN2-mitochondria; LAMP2-lysosome; Lamin B1- nucleus; PDI- endoplasmic reticulum (ER). Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/19264), licensed under a CC-BY license.$

Western Blot: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Representative pictures of effect of I/R on DNA damage, cell survival, and inflammation. Western blot analysis expression level of Cathepsin B (*P < 0.05) in total protein extract from RVA of I/R treated vs. control group. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fphar.2018.01034/full), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-LAMP-2/CD107b [NBP2-22217] at a 1:100 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using anti-LAMP-2/CD107b (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.

ELISA: LAMP-2/CD107b Antibody (H4B4) [NBP2-22217] - Anti-LAMP-2/CD107b was used to assess lysates from human SVG-A cells via ELISA, by loading the indicated mass of total protein per well. Antibodies tested were diluted 1:250, and used in conjunction with an HRP-conjugated secondary antibody. Signal was detected by luminescence. Image from verified customer review.

LAMP-2/CD107b (H4B4) was detected in immersion fixed HeLa human cervix adenocarcinoma cell line using Mouse anti-LAMP-2/CD107b (H4B4) Protein G-purified Monoclonal Antibody (Catalog # NBP2-22217) at 1.0 µg/mL overnight at 4C. Cells were stained using DyLight 488-conjugated Anti-Mouse IgG (H+L) Cross-Absorbed Secondary Antibody (green), and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

LAMP-2/CD107b (H4B4) was detected in immersion fixed MCF7 human breast cancer cell line using Mouse anti-LAMP-2/CD107b (H4B4) Protein G-purified Monoclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NBP2-22217AF647) (light blue) at 5 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Reactivity:

Hu, Mu, Ch, Rt(-)

Species Glossary

Reactivity: Hu, Mu, Ch, Rt(-)

Applications:

, , , , , , , ,

Applications: , , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-22217AF350
Alexa Fluor 405	NBP2-22217AF405
Alexa Fluor 488	NBP2-22217AF488
Alexa Fluor 532	NBP2-22217AF532
Alexa Fluor 594	NBP2-22217AF594
Alexa Fluor 647	NBP2-22217AF647
Alexa Fluor 700	NBP2-22217AF700
Alexa Fluor 750	NBP2-22217AF750
Allophycocyanin	NBP2-21799
Allophycocyanin	NBP2-22217APC
Allophycocyanin/Cy7	NBP2-22217APCCY7
Biotin	NBP2-22217B
CoraFluor 1	NBP2-22217CL1
DyLight 350	NBP2-22217UV
DyLight 405	NBP2-22217V
DyLight 488	NBP2-22217G
DyLight 550	NBP2-22217R
DyLight 594	NBP2-22217DL594
DyLight 650	NBP2-22217C
DyLight 680	NBP2-22217FR
DyLight 755	NBP2-22217IR
FITC	NBP1-28553
FITC	NBP2-22217F
HRP	NBP2-22217H
Janelia Fluor 525	NBP2-22217JF525
Janelia Fluor 549	NBP2-22217JF549
Janelia Fluor 646	NBP2-22217JF646
Janelia Fluor 669	NBP2-22217JF669
PE	NBP2-22217PE
PE/Atto594	NBP2-22217PEATT594
PE/Cy5.5	NBP2-22217PECY55
PE/Cy7	NBP2-22217PECY7
PerCP	NBP2-22217PCP
mFluor Violet 450 SE	NBP2-22217MFV450
mFluor Violet 500 SE	NBP2-22217MFV500
mFluor Violet 610 SE	NBP2-22217MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-22217SS
Azide and BSA Free	NBP2-80825

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NBP2-25208

B7-2/CD86 Antibody (BU63)

(34 Publications)

0.1 mg

NBP2-25208

Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Immunofluorescence staining of PFA-fixed Ramos cells using followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.

Immunohistochemistry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Effects of 20(S)-Rg3 on macrophage polarization in atherosclerotic lesions of diabetic ApoE-/- mice. Co-immunofluorescence staining for macrophage (anti-Moma-2 antibody, green), M1 marker CD86 (red) and DAPI (blue) (n = 5, respectively). Scale bar: 20 um. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/articles/10.3389/fphar.2018.00464/full) licensed under a CC-BY license.

Flow Cytometry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Flow Cytometric Analysis of PFA-fixed Ramos cells. B7-2/CD86 Antibody (BU63) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Formalin-fixed, paraffin-embedded esophagus tumor tissue stained with CD86 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Note strong staining of differentiated squamous cells. TMA was used for this test.

Flow Cytometry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Flow Cytometric Analysis of human PBMCs using B7-2/CD86 Antibody (BU63); Goat anti-Mouse IgG-CF488 (red); Isotype Control (green).

Immunohistochemistry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Macrophage polarization states. Distribution & proportion of CD68+ (a), CD86+ (c) & CD163+ (e) macrophages (brown) were identified using IHC procedures. Intensity for the color response to antibodies was visible in lung tissues from all groups. Population proportions for the stained cells as shown by red arrows were calculated as a fold change of the control. (b, d, f). The results were expressed as Mean ± SD (n = 3). **: a p-value of < 0.01 vs NS, OVA/BUD & OVA/PCI except the number of CD86+ cells in OVA/PCI group. #: P < 0.05 vs NS & ##; P < 0.01 vs either NS or OVA/BUD group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32111211), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Effects of 20(S)-Rg3 on macrophage polarization in atherosclerotic lesions of diabetic ApoE–/– mice. (A,B) Co-immunofluorescence staining of the aortic root for macrophage (anti-Moma-2 antibody, green) & M1 marker iNos (red), & a bar graph summarizing the results (n = 5, respectively). (C,D) Co-immunofluorescence staining for macrophage (anti-Moma-2 antibody, green) & M1 marker CD86 (red), & a bar graph summarizing the results (n = 5, respectively). Scale bar: 20 μm. Data are mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29867472), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

M1 macrophages were induced in the in vivo mouse intervertebral disc (mIVD) degeneration model (puncture model). (a) Immunohistological analysis of Iba-1, iNOS, and arginase (brown staining) expression in nucleus pulposus (NP), annulus fibrosis (AF), and cartilage endplate (CEP) [right] at high magnification and in whole mIVDs (left) at low magnification. Images using an IgG isotype control antibody are included to demonstrate the specificity of the IHC staining. (b) Protein was extracted from punctured mIVDs in the puncture model, and western blotting was performed using antibodies against CD86, CD163, and beta -actin. (c) Images of the panel in (b), captured using a ChemiDoc Touch system and quantified using ImageJ. Biological and technical replicates were performed three times. Values represent the mean +/- SD. *p < 0.05, **p < 0.01, relative to the corresponding control. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/41053148), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Thrombin promotes polarization into M1 macrophages. (a) Mouse bone marrow was stimulated with thrombin, and quantitative PCR was performed using specific primers for CD86 and CD163. Biological and technical replicates were performed three times. (b) Mouse bone marrow was stimulated with thrombin, and western blotting was performed using antibodies for the M1 markers iNOS and CD86, and the M2 markers CD163 and Arginase-1. beta -actin was used as an internal control. n = 3. Values represent the mean +/- SD. *p < 0.05, **p < 0.01, relative to the corresponding control. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/41053148), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

IGU alleviates the inflammation of type II alveolar epithelial cells caused by M1 macrophages. (A–C) Representative western blot and quantitation for iNOS and CD86 in Raw264.7 cells. beta -actin was used as an internal control. (D) QRT-PCR for the mRNA levels of M1 macrophage polarization-related inflammatory cytokines TNF-alpha , IL-6 and IL-1 beta in Raw264.7 cells. (E, F) ELISA was used to measure the level of TNF-alpha and IL-6 in Raw264.7 cell culture supernatant. (G, H) QRT-PCR for the mRNA levels of MMP2 and MMP9. (I) Representative gelatin zymogram of MMPs in Raw264.7 cell culture supernatants. (J) Schematic representation of Raw264.7 cells co-cultured with type II alveolar epithelial cells. (K–M) QRT-PCR for the mRNA levels of TNF-alpha , IL-6 and IL-1 beta in type II alveolar epithelial cells (n=3). *P<0.05, **P<0.01, ***P<0.001, ns, No significance. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40181990), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Rescue experiments of Raw264.7 cells after overexpression of TLR4. (A, B) Representative western blot and quantitation of TLR4 in TLR4-overexpressing Raw264.7 cells. beta -actin was used as an internal control. (C–E) QRT-PCR for the mRNA levels of M1 macrophage polarization-related inflammatory cytokines TNF-alpha , IL-6 and IL-1 beta in Raw264.7 cells. (F–J) Representative western blot and quantitation of protein levels of TLR4, NF-kappa B, iNOS and CD86 in Raw264.7 cells beta -actin was used as an internal control. (n=3). *P<0.05, **P<0.01, ***P<0.001, ns, No significance. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40181990), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-6882A

Alt Catalog# IMG-6882A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-34569AF350
Alexa Fluor 405	NBP2-34569AF405
Alexa Fluor 488	NBP2-34569AF488
Alexa Fluor 532	NBP2-34569AF532
Alexa Fluor 594	NBP2-34569AF594
Alexa Fluor 647	NBP2-34569AF647
Alexa Fluor 700	NBP2-34569AF700
Alexa Fluor 750	NBP2-34569AF750
Allophycocyanin	NBP2-34569APC
Allophycocyanin/Cy7	NBP2-34569APCCY7
Biotin	NBP2-34569B
CoraFluor 1	NBP2-34569CL1
DyLight 350	NBP2-34569UV
DyLight 405	NBP2-34569V
DyLight 488	NBP2-34569G
DyLight 550	NBP2-34569R
DyLight 594	NBP2-34569DL594
DyLight 650	NBP2-34569C
DyLight 680	NBP2-34569FR
DyLight 755	NBP2-34569IR
FITC	NBP2-34569F
HRP	NBP2-34569H
Janelia Fluor 525	NBP2-34569JF525
Janelia Fluor 549	NBP2-34569JF549
Janelia Fluor 646	NBP2-34569JF646
Janelia Fluor 669	NBP2-34569JF669
PE	NBP2-34569PE
PE/Atto594	NBP2-34569PEATT594
PE/Cy5.5	NBP2-34569PECY55
PE/Cy7	NBP2-34569PECY7
PerCP	NBP2-34569PCP
mFluor Violet 450 SE	NBP2-34569MFV450
mFluor Violet 500 SE	NBP2-34569MFV500
mFluor Violet 610 SE	NBP2-34569MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
IHC-Prediluted	NBP2-44513
Azide and BSA Free	NBP2-34569-0.1mg

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NB100-65805

CD81 Antibody (1D6) - BSA Free

(1 Review)

(26 Publications)

0.1 ml

NB100-65805

Flow Cytometry: CD81 Antibody (1D6) - BSA Free [NB100-65805] - A surface stain was performed on Jurkat cells with CD81 Antibody (1D6) NB100-65805 (blue) and a matched isotype control MAB002 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).

Western Blot: CD81 Antibody (1D6) [NB100-65805] - Analysis of human testis tissue (A) using CD81 antibody at a concentration of 2 ug/mL.

Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - Surface flow cytometric staining of 1 x 10^6 PBMC cells using CD81 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1 x 10^6 cells was used.

Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - Staining of human peripheral blood lymphocytes.

Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - Analysis using the FITC conjugate of NB100-65805. Staining of human peripheral blood lymphocytes.

Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - A surface stain was performed on Jurkat cells with CD81 (1D6) antibody NB100-65805AF647(blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - A surface stain was performed on Jurkat Cells with CD81 Antibody (1D6) NB100-65805B and a matched isotype control. Both antibodies were conjugated to biotin. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).

Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - A surface stain was performed on Jurkat cells with CD81 Antibody [1D6] NB100-65805AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Western Blot: CD81 Antibody (1D6) - BSA Free [NB100-65805] - Characterization & internalization of M2-Exos. (A) TEM image of M2-Exos. Scale bar: 200 nm. (B) DLS measurement of M2-Exos size. (C) Western blotting assay of exosomal markers in THP-1-M2 cellular lysate & M2-Exos preparation. (D,F) Fluorescence images of 786-O & ACHN cells treated with or without PKH67-labeled M2-Exos (green). Scale bar: 50 μm. (E,G) Three-dimensional confocal reconstruction of 786-O & ACHN cells treated with PKH67-labeled M2-Exos (green). (H) Fluorescence staining analyzing the internalization of M2-Exos by 786-O cells over 12 h. Scale bar: 10 μm. CT: control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35328425), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Principle of EV‐GLYPH assay for early‐stage NSCLC identification. A) Glycan signatures on sEVs derived from patients with benign and malignant lung diseases. B) CT and PET images of benign and malignant lung nodules. C) Working scheme of EV‐GLYPH assay. SEC‐purified sEVs from plasma are captured by anti‐MUC1 antibody immobilized on an electrode and subsequently labeled with SERS nanotags against LacdiNAc, T antigen, and CD81. D) A nanomixing fluid flow is generated on the electrode surface by the applied ac‐EHD field. E) With in situ SERS mapping, the average Raman intensities and false‐color SERS spectral images (insets) are established based on the characteristic Raman signals of three SERS nanotags (WFA‐MBA, 1075 cm−1, red; PNA‐TFMBA, 1375 cm−1, blue; anti‐CD81‐DTNB, 1335 cm−1, green), representing the expression of LacdiNAc, T antigen, and CD81, respectively. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38885350), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Gt, Pm, Sh

Species Glossary

Reactivity: Hu, Mu, Gt, Pm, Sh

Applications:

, , , , , , , ,

Applications: , , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-65805AF350
Alexa Fluor 405	NB100-65805AF405
Alexa Fluor 488	NB100-65805AF488
Alexa Fluor 532	NB100-65805AF532
Alexa Fluor 594	NB100-65805AF594
Alexa Fluor 647	NB100-65805AF647
Alexa Fluor 700	NB100-65805AF700
Alexa Fluor 750	NB100-65805AF750
Allophycocyanin	NB100-65805APC
Allophycocyanin/Cy7	NB100-65805APCCY7
Biotin	NB100-65805B
CoraFluor 1	NB100-65805CL1
DyLight 350	NB100-65805UV
DyLight 405	NB100-65805V
DyLight 488	NB100-65805G
DyLight 550	NB100-65805R
DyLight 594	NB100-65805DL594
DyLight 650	NB100-65805C
DyLight 680	NB100-65805FR
DyLight 755	NB100-65805IR
FITC	NB100-65805F
FITC	NB100-64060
HRP	NB100-65805H
Janelia Fluor 525	NB100-65805JF525
Janelia Fluor 549	NB100-65805JF549
Janelia Fluor 646	NB100-65805JF646
Janelia Fluor 669	NB100-65805JF669
PE	NB100-65805PE
PE/Atto594	NB100-65805PEATT594
PE/Cy5.5	NB100-65805PECY55
PE/Cy7	NB100-65805PECY7
PerCP	NB100-65805PCP
mFluor Violet 450 SE	NB100-65805MFV450
mFluor Violet 500 SE	NB100-65805MFV500
mFluor Violet 610 SE	NB100-65805MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-65805SS
Azide and BSA Free	NBP2-80660

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NB110-40686

CD163 Antibody (EDHu-1) - BSA Free

(4 Reviews)

(28 Publications)

0.1 mg

NB110-40686

CD163 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human CD163 Monoclonal Antibody (Novus Catalog # NB110-40686) at 1:50 at 37 °Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cytoplasm. Protocol available in COMET™ Panel Builder.

CD163 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD163 Monoclonal Antibody (Novus Catalog # NB110-40686) at 20 µg/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 8 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cytoplasm. Protocol available in COMET™ Panel Builder.

CD163 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CD163 Monoclonal Antibody (Novus Catalog # NB110-40686) at 1:50 at 37 °Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cytoplasm. Protocol available in COMET™ Panel Builder.

Non-repaired and repaired VML injured muscles present sustained putative macrophage infiltration. Representative histologic images labeled with CD163 (pan macrophage marker, green) and wheat germ agglutinin (WGA, red) and DAPI (nuclei, blue). Examples for each group, specifically the non-, SIS-, UMB-, and Hya-reparied are shown. Scale bar is 150 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-13306-2), licensed under a CC-BY license.

A surface stain was performed on hPBMCs with CD163 (EDHu-1) antibody NB110-40686AF700 and a matched isotype control NBP2-27287AF700. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. A co-stain was performed with NB100-77758AF488. Image using the Alexa Fluor 700 form of this antibody.

Staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1.

A surface stain was performed on human peripheral blood monocytes with CD163 (EDHu-1) antibody NB110-40686APC and a matched isotype control NBP2-27287APC. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. A co-stain was performed with NB100-77758AF488.

FFPE human tonsil, stained with anti-CD163, pH 9 antigen retrieval. IHC-P Image submitted by a verified customer review.

A surface stain was performed on hPBMCs with CD163 (EDHu-1) antibody NB110-40686PE and a matched isotype control NBP2-27287PE. Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. A co-stain was performed with NB100-77758APC. Image using the PE form of this antibody.

Human tonsil cryosection with Mouse anti Human CD163 antibody, clone, red in A and Mouse anti Human CD21 antibody, green in B. C is the merged image with nuclei counterstained blue using DAPI.

Frozen human skin sections. CD163 (green) in control human skin. Sections were fixed with 4% PFA PBS, permeabilized wtih 0.5% Triton TBS Ca/Azide, and blocked with a 5% BSA 0.05% T20 TBS Ca/Azide solution. Antibody was diluted 1:100 and applied for 1 hr at 37C. Detection was performed with anti-host conjugated antibody, and sections were counterstained with DAPI. IHC-Fr image submitted by a verified customer review.

Immunohistochemistry-Frozen: CD163 Antibody (EDHu-1) [NB110-40686] - Staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1.

Immunohistochemistry-Paraffin: Mouse Monoclonal CD163 Antibody (EDHu-1) [NB110-40686] - CD163 immunoreactivity in a formalin fixed paraffin embedded section of canine lymph node. NB110-40686 was diluted to 0.25ug per mL and was left on the tissue section for 30m at room temperature. Section underwent 20m of heat induced epitope retrieval in a vegetable steamer using a citrate buffer. No signal was detected in sections that did not undergo HIER. Image from a verified customer review.

Immunohistochemistry-Paraffin: Mouse Monoclonal CD163 Antibody (EDHu-1) [NB110-40686] - CD163 immunoreactivity in an FFPE section of human tonsil. NB110-40686 was diluted to 250 ng/mL and left on tissue sections for 30min at room temperature. Secondary was horse anti mouse HRP polymer. Image from a verified customer review.

Immunohistochemistry-Paraffin: Mouse Monoclonal CD163 Antibody (EDHu-1) [NB110-40686] - CD163 immunoreactivity in an FFPE section of equine spleen. NB110-40686 was diluted to 250ng per mL and left on tissue sections for 30min at room temperature. Secondary was horse anti mouse HRP polymer. Image from a verified customer review.

Immunocytochemistry/ Immunofluorescence: CD163 Antibody (EDHu-1) [NB110-40686] - Non-repaired & repaired VML injured muscles present sustained putative macrophage infiltration. Representative histologic images labeled with CD163 (pan macrophage marker) & wheat germ agglutinin (WGA) & DAPI (nuclei). (a) For each of the sham muscle sections individual & merged images are presented. (b) Examples for each group, specifically the non-, SIS-, UMB-, & Hya-reparied are shown. Scale bar is 150 µm; all images are at the same magnification & follow the same location pattern displayed specifically in Fig. 4e. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29030619), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-40686AF350
Alexa Fluor 405	NB110-40686AF405
Alexa Fluor 488	NB110-40686AF488
Alexa Fluor 532	NB110-40686AF532
Alexa Fluor 594	NB110-40686AF594
Alexa Fluor 647	NB110-40686AF647
Alexa Fluor 700	NB110-40686AF700
Alexa Fluor 750	NB110-40686AF750
Allophycocyanin	NB110-40686APC
Allophycocyanin/Cy7	NB110-40686APCCY7
Biotin	NB110-40686B
CoraFluor 1	NB110-40686CL1
DyLight 350	NB110-40686UV
DyLight 405	NB110-40686V
DyLight 488	NB110-40686G
DyLight 550	NB110-40686R
DyLight 594	NB110-40686DL594
DyLight 650	NB110-40686C
DyLight 680	NB110-40686FR
DyLight 755	NB110-40686IR
FITC	NB110-40686F
HRP	NB110-40686H
Janelia Fluor 525	NB110-40686JF525
Janelia Fluor 549	NB110-40686JF549
Janelia Fluor 646	NB110-40686JF646
Janelia Fluor 669	NB110-40686JF669
PE	NB110-40686PE
PE/Atto594	NB110-40686PEATT594
PE/Cy5.5	NB110-40686PECY55
PE/Cy7	NB110-40686PECY7
PerCP	NB110-40686PCP
mFluor Violet 450 SE	NB110-40686MFV450
mFluor Violet 500 SE	NB110-40686MFV500
mFluor Violet 610 SE	NB110-40686MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-40686-0.025mg

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NB600-534

Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free

(1 Review)

(25 Publications)

0.1 ml

NB600-534

Immunohistochemistry-Paraffin: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Staining of Sialoadhesin in human spleen using DAB with hematoxylin counterstain.

Reactivity:

Hu, Mu, Po, V-Vi

Species Glossary

Reactivity: Hu, Mu, Po, V-Vi

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-534AF350
Alexa Fluor 405	NB600-534AF405
Alexa Fluor 488	NB600-534AF488
Alexa Fluor 532	NB600-534AF532
Alexa Fluor 594	NB600-534AF594
Alexa Fluor 647	NB600-534AF647
Alexa Fluor 700	NB600-534AF700
Alexa Fluor 750	NB600-534AF750
Allophycocyanin	NB600-534APC
Allophycocyanin/Cy7	NB600-534APCCY7
Biotin	NB600-534B
CoraFluor 1	NB600-534CL1
DyLight 350	NB600-534UV
DyLight 405	NB600-534V
DyLight 488	NB600-534G
DyLight 550	NB600-534R
DyLight 594	NB600-534DL594
DyLight 650	NB600-534C
DyLight 680	NB600-534FR
DyLight 755	NB600-534IR
FITC	NB600-534F
HRP	NB600-534H
Janelia Fluor 525	NB600-534JF525
Janelia Fluor 549	NB600-534JF549
Janelia Fluor 646	NB600-534JF646
Janelia Fluor 669	NB600-534JF669
PE	NB600-534PE
PE/Atto594	NB600-534PEATT594
PE/Cy5.5	NB600-534PECY55
PE/Cy7	NB600-534PECY7
PerCP	NB600-534PCP
mFluor Violet 450 SE	NB600-534MFV450
mFluor Violet 500 SE	NB600-534MFV500
mFluor Violet 610 SE	NB600-534MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB600-534SS
Azide and BSA Free	NBP2-80947

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NB200-585

TfR (Transferrin R) Antibody (OX26) - BSA Free

(18 Publications)

0.1 mg

NB200-585

Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody (OX26) [NB200-585] - Analysis of FFPE mouse spleen using TfR (OX26) antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Plasma membrane staining was observed. Staining was performed by Histowiz.

Flow Cytometry: TfR (Transferrin R) Antibody (OX26) [NB200-585] - Analysis using the Biotin conjugate of NB200-585. Staining of mouse spleen.

Flow Cytometry: TfR (Transferrin R) Antibody (OX26) [NB200-585] - Staining of rat spleen lymphocytes with mouse anti rat CD71.

Flow (Intracellular): TfR (Transferrin R) Antibody (OX26) [NB200-585] - PC-12 cells were stained with NB200-585 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by DyLight488-conjugated anti-mouse secondary antibody.

Flow Cytometry: TfR (Transferrin R) Antibody (OX26) [NB200-585] - Staining of rat spleen cells with mouse anti rat CD71: RPE.

Flow Cytometry: TfR (Transferrin R) Antibody (OX26) [NB200-585] - An intracellular stain was performed on PC12 cells with TfR (Transferrin R) [OX26] Antibody NB200-585AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

$Protein level and subcellular content analysis of v-SNARE and Rab proteins in mice crypts. (A) Representative image of 5~50% Optiprep gradient of ep/+ and ep mice small intestine crypts. VAMP7 mainly appears at the 11th and 12th fractions labeled by lysozyme and endosomal component TfR and a second peak appears at the first two fractions labeled by GM130 and TGN38, while it increases in the 4th fraction in ep crypts. Rab38 mainly appears at the first two fractions of both genotypes while its concentration increases in the middle fragments in ep crypts. Rab32 has similar distributional pattern as Rab38 but more widely distributed in 3rd~6th fractions, but less in the 11th fraction labeled by TfR in ep crypts. (B) Statistical analysis of Rab38 distribution in (A). In ep crypt, the increase of Rab38 in the middle density fragments is significant (the 3rd~5th fractions, ep/+ crypts 9%, ep crypts 16%, *p < 0.05). (C) Statistical analysis of Rab32 distribution in (D). Rab32 in ep crypts significantly decreases at the low density fragment (the 1st fraction: ep/+ crypts 25%, ep crypts 16%, *p < 0.05) and at the 9th fraction (ctrl 4%, ep 2%, *p < 0.05). (D) Statistical analysis of VAMP7 distribution in (A). VAMP7 in ep crypts significantly increases in TGN38 and GM130 enriched fraction (the 2nd fraction, ctrl crypts 17%, ep crypts 28%, *p < 0.05) and the 4th fraction (ep/+ crypts 2%, ep crypts 6%, **p < 0.01), and decreases in the RE enriched 11th fraction (ep/+ crypts 18%, ep crypts 5%, *p < 0.05). 4 mice of each genotype were analyzed, values are means +/- s.e.m. of three replicates of immunoblotting data. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33224134), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Reactivity:

Mu, Rt

Species Glossary

Reactivity: Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB200-585AF350
Alexa Fluor 405	NB200-585AF405
Alexa Fluor 488	NB200-585AF488
Alexa Fluor 532	NB200-585AF532
Alexa Fluor 594	NB200-585AF594
Alexa Fluor 647	NB200-585AF647
Alexa Fluor 700	NB200-585AF700
Alexa Fluor 750	NB200-585AF750
Allophycocyanin	NB200-585APC
Biotin	NB200-585B
CoraFluor 1	NB200-585CL1
DyLight 350	NB200-585UV
DyLight 405	NB200-585V
DyLight 488	NB200-585G
DyLight 550	NB200-585R
DyLight 594	NB200-585DL594
DyLight 650	NB200-585C
DyLight 680	NB200-585FR
DyLight 755	NB200-585IR
FITC	NB200-585F
HRP	NB200-585H
Janelia Fluor 525	NB200-585JF525
Janelia Fluor 549	NB200-585JF549
Janelia Fluor 646	NB200-585JF646
Janelia Fluor 669	NB200-585JF669
PE	NB200-585PE
PerCP	NB200-585PCP
mFluor Violet 450 SE	NB200-585MFV450
mFluor Violet 500 SE	NB200-585MFV500
mFluor Violet 610 SE	NB200-585MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB200-585SS
Azide and BSA Free	NBP2-80976

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NBP2-22541

ICAM-1/CD54 Antibody (1A29) - Azide and BSA Free

(1 Review)

(19 Publications)

0.1 mg

NBP2-22541

Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Analysis of CD54 in C6 cells (green) compared to an isotype control (blue).

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Normal biopsies of deparaffinized Mouse kidney tissue.

Immunohistochemistry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Normal biopsies of deparaffinized Mouse lung tissue.

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Normal biopsies of deparaffinized Mouse spleen tissue.

Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Staining of stimulated rat spleen cells with mouse anti-rat CD54 (Alexa488).

Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Analysis of CD54 in Ramos cells (green) compared to an isotype control (blue).

Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Analysis of CD54 in Raji cells (green) compared to an isotype control (blue).

<b>Genetic Strategies Validation. </b>Knockdown of RPN2 decreased luminal B BC cell dissemination in vivo. Luminal B ER+ T47D cells, transfected with negative control siRNA (siRNA-C) or siRNA targeting RPN2 (siRNA-RPN2) were injected + estradiol (E2) ± neutrophils (Neu) into zebrafish transgenic embryos with green fluorescent blood vessels & analyzed as described in materials & methods. (A) Migration in vitro (n = 6). (B)In vivo dissemination of transfected T47D in presence of E2 ± Neu (n = 23–26). Scale bar = 100 µm. (n = 23–26). (C) Western blot analysis for confirmation of siRNA-RPN2 transfection & VCAM-1, ICAM-1, & MUC-1 expression. (D) Focal adhesions area (n = 5–6). Scale bar = 10 µm. (E) Proliferation (n = 6). Representative images of zebrafish embryos with disseminated luminal B T47D BC cells & immunocytochemistry analysis of vinculin expression are shown. Arrows show disseminated T47D & arrowheads show focal adhesions. BV = blood vessels. Data are presented as mean ± SEM. Two-tailed Student’s t-test *P < 0.05, ***P < 0.001, ns, not significant. Data are represented of at least two independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33330095), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

<b>Genetic Strategies Validation. </b>Knockdown of U2AF1 decreased luminal A BC cell dissemination in vivo. Luminal A ER+ MCF-7 cells, transfected with negative control siRNA (siRNA-C) or siRNA targeting U2AF1 (siRNA-U2AF1) were injected in presence of estradiol (E2) ± neutrophils (Neu) into zebrafish transgenic embryos, with green fluorescent blood vessels, & analyzed as described in materials & methods. (A) Migration in vitro (n = 6–12). (B)In vivo dissemination in presence of E2 ± Neu (n = 38–41). Scale bar = 100 µm. (C) Western blot analysis for confirmation of siRNA-U2AF1 transfection & ICAM-1, VCAM-1, & MUC-1 expression. (D) Focal adhesion area (n = 7). Scale bar = 10 µm. (E) Proliferation in vitro (n = 12). Representative images of zebrafish embryos with disseminated MCF-7 cells & immunocytochemistry analysis of vinculin expression are shown. Arrows show disseminated MCF-7 cells & arrowheads show focal adhesions. BV = blood vessels. Data are presented as mean ± SEM. Two-tailed Student’s t-test *P < 0.05, **P < 0.01, ns, not significant. Data are represented of at least two independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33330095), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Normalized gene & protein expression of ICAM-1. (A) qPCR results indicated no change in mRNA expression of ICAM-1 at 24 h post-overpressure but a significant increase at 48 h as compared to sham. (B) Subsequent Western blot results confirmed elevated ICAM-1 protein levels at 48 h compared to sham. *p < 0.05, Data are represented as mean ± SEM, gene: n = 7–9/group, protein: n = 8–9/group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30853931), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Western Blot: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Fractionated, compared to single exposure, radiation more profoundly enhanced ICAM-1 expression. Representative (3 independent experiments) Western blot analysis & quantification of ICAM-1 levels in whole-cell lysates from nonirradiated (sham) & irradiated HUVECs 4 h & 24 h after exposure to (a) either five fractions of 2 Gy (5 × 2 Gy) or single exposure to 10 Gy & (b) either five fractions of 2.5 Gy (5 × 2.5 Gy) or single exposure to 12.5 Gy. Fractions delivered at 24-h intervals. beta -actin served as a loading control. c Ectopic expression of ICAM-1 after exposure to 0 Gy, five fractions of 2 Gy, or 10 Gy, as measured by flow cytometry (n = 3). d NF-kappa B activation after 4 h of exposure to 0 Gy, five fractions of 2 Gy, or 10 Gy (n = 2). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32382091), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Western Blot: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Fractionated thoracic irradiation suppressed KLF2 & enhanced ICAM-1 levels in the lung. Quantification of KLF2 (a) & ICAM-1 (b) protein levels & representative Western blot analysis (c) in the lung tissue of mice (n = 6) at 24 h following 5 fractions of 4 Gy at 24 h intervals or single exposure to 20 Gy. beta -actin served as a loading control. KLF2 immunostaining in the lung tissue samples of sham irradiated (d), irradiated (e), & quantitation (f) at 24 h after exposure to 5 fractions of 4 Gy at 24 h intervals or single exposure to 20 Gy. ICAM-1 immunostaining in the lung tissue samples of sham irradiated (g), or irradiated (h), & quantitation (i) at 24 h after exposure to 5 fractions of 4 Gy at 24 h intervals or single exposure to 20 Gy. (n, number of independent experiments performed; a, significant statistical difference between nonirradiated & irradiated groups; b, significant statistical difference between fractionated irradiation & single exposure; *p < 0.05; **p < 0.01; ***p < 0.001). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32382091), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , , , , ,

Applications: , , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-22541AF350
Alexa Fluor 405	NBP2-22541AF405
Alexa Fluor 488	NBP2-22541AF488
Alexa Fluor 532	NBP2-22541AF532
Alexa Fluor 594	NBP2-22541AF594
Alexa Fluor 647	NBP2-22541AF647
Alexa Fluor 700	NBP2-22541AF700
Alexa Fluor 750	NBP2-22541AF750
Allophycocyanin	NBP2-22541APC
Allophycocyanin/Cy7	NBP2-22541APCCY7
Biotin	NBP2-22541B
CoraFluor 1	NBP2-22541CL1
DyLight 350	NBP2-22541UV
DyLight 405	NBP2-22541V
DyLight 488	NBP2-22541G
DyLight 550	NBP2-22541R
DyLight 594	NBP2-22541DL594
DyLight 650	NBP2-22541C
DyLight 680	NBP2-22541FR
DyLight 755	NBP2-22541IR
FITC	NBP2-22541F
HRP	NBP2-22541H
Janelia Fluor 525	NBP2-22541JF525
Janelia Fluor 549	NBP2-22541JF549
Janelia Fluor 646	NBP2-22541JF646
Janelia Fluor 669	NBP2-22541JF669
PE	NBP2-22541PE
PE/Atto594	NBP2-22541PEATT594
PE/Cy5.5	NBP2-22541PECY55
PE/Cy7	NBP2-22541PECY7
PerCP	NBP2-22541PCP
mFluor Violet 450 SE	NBP2-22541MFV450
mFluor Violet 500 SE	NBP2-22541MFV500
mFluor Violet 610 SE	NBP2-22541MFV610

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NB100-92243

TfR (Transferrin R) Antibody - BSA Free

(1 Review)

(19 Publications)

0.1 ml

NB100-92243

Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody - BSA Free [NB100-92243] - Analysis of a FFPE tissue section of human placenta using 1:200 dilution of TfR (Transferrin R) antibody NB100-92243. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Western Blot: TfR (Transferrin R) Antibody [NB100-92243] - Analysis of Transferrin Receptor Antibody in extracts from 293 cells.

Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody [NB100-92243] - Paraffin-embedded human colorectal carcinoma tissue at 1:50.

TfR (Transferrin R) was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Rabbit anti- TfR (Transferrin R) Affinity Purified Polyclonal Antibody conjugated to Alexa Fluor® 488 (Catalog # NB100-92243AF488) (green) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (dark blue). Cells were imaged using a 100X objective and digitally deconvolved.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-92243AF350
Alexa Fluor 405	NB100-92243AF405
Alexa Fluor 488	NB100-92243AF488
Alexa Fluor 532	NB100-92243AF532
Alexa Fluor 594	NB100-92243AF594
Alexa Fluor 647	NB100-92243AF647
Alexa Fluor 700	NB100-92243AF700
Alexa Fluor 750	NB100-92243AF750
Allophycocyanin	NB100-92243APC
Biotin	NB100-92243B
CoraFluor 1	NB100-92243CL1
DyLight 350	NB100-92243UV
DyLight 405	NB100-92243V
DyLight 488	NB100-92243G
DyLight 550	NB100-92243R
DyLight 594	NB100-92243DL594
DyLight 650	NB100-92243C
DyLight 680	NB100-92243FR
DyLight 755	NB100-92243IR
FITC	NB100-92243F
HRP	NB100-92243H
Janelia Fluor 525	NB100-92243JF525
Janelia Fluor 549	NB100-92243JF549
Janelia Fluor 646	NB100-92243JF646
Janelia Fluor 669	NB100-92243JF669
PE	NB100-92243PE
PerCP	NB100-92243PCP
mFluor Violet 450 SE	NB100-92243MFV450
mFluor Violet 500 SE	NB100-92243MFV500
mFluor Violet 610 SE	NB100-92243MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-92243SS

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Kit Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

TLR9 Antibody (26C593.2) - BSA Free

CD36 Antibody - BSA Free

CD63 Antibody (H5C6) - BSA Free

FPRL1/FPR2 Antibody - BSA Free

LAMP-2/CD107b Antibody (H4B4) - BSA Free

B7-2/CD86 Antibody (BU63)

CD81 Antibody (1D6) - BSA Free

CD163 Antibody (EDHu-1) - BSA Free

Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free

TfR (Transferrin R) Antibody (OX26) - BSA Free

ICAM-1/CD54 Antibody (1A29) - Azide and BSA Free

TfR (Transferrin R) Antibody - BSA Free