FPRL1/FPR2 Antibody

Images

 
Western Blot: FPRL1/FPR2 Antibody [NLS1878] - Immunoblot of FPR2 protein in various parts of the rat brain including olfactory bulb (OB), prefrontal cortex (PFC), primary somatosensory cortex (CTX1), parietal ...read more
Immunocytochemistry/ Immunofluorescence: FPRL1/FPR2 Antibody [NLS1878] - Mouse J774A.1, mouse reticulum cell sarcoma macrophage cell line. Left panel is FPRL1/FPR2 Antibody + Anti-Rabbit FITC; middle panel indicates the ...read more
Immunohistochemistry-Paraffin: FPRL1/FPR2 Antibody [NLS1878] - SPM receptors are differentially expressed on the endothelium and the infiltrating leukocytes AE" ALX/FPR2 and ChemR23. Acute inflammation was triggered in ...read more
Flow Cytometry: FPRL1/FPR2 Antibody [NLS1878] - Flow Cytometry: FPRL1/FPR2 Antibody [Alexa Fluor® 700] [NLS1878AF700] - Mouse splenocytes. Cells were pre-gated with live/dead and FSC-A/W to exclude dead cells and ...read more
Western Blot: FPRL1/FPR2 Antibody [NLS1878] - Analysis in HL-60 cell lysate.
Western Blot: FPRL1/FPR2 Antibody [NLS1878] - Mouse bone marrow cell lysate with DMSO (lane 1), chemical agonist #1 (lane 2), chemical agonist #2 (lane 3), chemical agonist #3 (lane 4). WB image submitted by a verfied ...read more
Immunocytochemistry/ Immunofluorescence: FPRL1/FPR2 Antibody [NLS1878] - Antibody was tested in Raw264.7 cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: FPRL1/FPR2 Antibody [NLS1878] - Analysis in human kidney cancer using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: FPRL1/FPR2 Antibody [NLS1878] - Electron micrographs of FPR2 immunostained sections from the prefrontal cortex. Immunostaining is mostly present in axon pre-terminals (PrT) that did not ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.1 mg/ml

FPRL1/FPR2 Antibody Summary

Immunogen
This FPRL1/FPR2 antibody is made to a synthetic peptide made to the 2nd extracellular loop of the human FPRL1 protein (between residues 300-350) [UniProt P25090]
Localization
Cell membrane; Multi-pass membrane protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FPR2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:20-1:75
  • Immunohistochemistry 1:300
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:300
Application Notes
This FPRL1 antibody is useful for Western Blot, ICC/IF, and IHC on paraffin embedded sections. In Western Blot, a band is seen ~38 kDa representing FPRL1. In ICC/IF, plasma membrane staining was observed in Raw264.7 cells. In IHC-P, staining was also observed in the plasma membrane of human kidney cancer tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Customers have reported success in IF on FFPE mouse kidney tissue, following microwave antigen retrieval. Use in frozen IHC sections reported in scientific literature (PMID: 24086560).
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
FPRL1/FPR2 Peptide (NLS1878PEP)
Reviewed Applications
Read 4 Reviews rated 4.5
using
NLS1878 in the following applications:

Publications
Read Publications using
NLS1878 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24086560). Bacteria reactivity reported in scientific literature (PMID: 31234710).

Packaging, Storage & Formulations

Storage
Store at -20 degrees C. Avoid freeze/thaw cycles.
Buffer
PBS, 30% Glycerol
Preservative
0.01% Sodium Azide
Concentration
1.1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FPRL1/FPR2 Antibody

  • ALXR
  • FMLP-R-I
  • FMLP-R-II
  • FMLPX
  • formyl peptide receptor 2
  • Formyl peptide receptor-like 1RFP
  • FPR2
  • FPR2A
  • FPRH1FMLP-related receptor I
  • FPRL1
  • FPRL1LXA4 receptor
  • HM63
  • HM63FPRH2
  • lipoxin A4 receptor (formyl peptide receptor related)
  • Lipoxin A4 receptor
  • LXA4 Receptor
  • LXA4RFMLP-R-I
  • N-formyl peptide receptor 2
  • RFP

Background

FPRL1 (formyl peptide receptor-like-1 or N-formyl peptide receptor 2) is a multi-pass membrane protein receptor belonging to the GPCR1 family and is expressed abundantly in lungs and neutrophils. Among human FPRs and its variants (FPRL1/FPR-like 1 and FPRL2/FPR-like 2), FPRL1 is less efficiently activated by N-fMLP (N-formylmethionylleucylphenylalanine) than FPR and certain agonists which bind FPRL1 with high affinity includes lipoxin A4 eicosanoid, annexin 1, uPAR, the V3 region of the HIV-1 envelope glycoprotein gp120, the acute-phase protein SAA, the 42-amino-acid form of beta-amyloid, human prion peptide, and cathelicidin LL-37.

FPRL1 binding to its agonists triggers the activation of intracellular signaling molecules including calcium, PKC isoforms, phospholipases A2 and D, and MAPKs/p38MAPK. FPRL1 activation may result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as leukotriene B4.

Expression of FPR1, FPRL1, and FPRL2 has recently been documented to enhance invasive potential of melanoma cells. Moreover, FPRL1 interacts with amyloid beta peptides is implicated in phagocyte attraction to sites of amyloid plaques in Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NLS1878
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Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for FPRL1/FPR2 Antibody (NLS1878)(20)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Bacteria.

We have publications tested in 6 applications: ICC/IF, IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(3)
IF
(1)
IHC
(1)
IHC-Fr
(3)
IHC-P
(2)
WB
(8)
All Applications
Filter By Species
Human
(5)
Mouse
(11)
Rat
(4)
Bacteria
(1)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using NLS1878 Applications Species
Kim H, Park SH, Han SY et al. LXA4-FPR2 signaling regulates radiation-induced pulmonary fibrosis via crosstalk with TGF-beta/Smad signaling Cell Death Dis Aug 8 2020 [PMID: 32811815] (WB, Human, Mouse) WB Human, Mouse
Tang D, Fu G, Li W et al. Maresin 1 Protects the Liver Against Ischemia/Reperfusion Injury via the ALXR/Akt Signaling Pathway Research Square Nov 11 2020 (WB, Mouse) WB Mouse
Ge Y, Zhang S, Wang J et al. Dual modulation of formyl peptide receptor 2 by aspirin-triggered lipoxin contributes to its anti-inflammatory activity FASEB J. Apr 2 2020 [PMID: 32239559]
Ren YZ, Zhang BZ, Zhao XJ, Zhang ZY Resolvin D1 ameliorates cognitive impairment following traumatic brain injury via protecting astrocytic mitochondria J. Neurochem. Jan 17 2020 [PMID: 31951012] (IHC-Fr, Mouse) IHC-Fr Mouse
Romp E, Arakandy V, Fischer J Exotoxins from Staphylococcus aureus activate 5-lipoxygenase and induce leukotriene biosynthesis Cell. Mol. Life Sci. Dec 5 2019 [PMID: 31807813] (WB, Human) WB Human
Sharba S, Venkatakrishnan V, Padra M et al. Formyl Peptide Receptor 2 Orchestrates Mucosal Protection Against Citrobacter rodentium Infection Virulence 2019 Jun 24 [PMID: 31234710] (IF, Bacteria)

Details:
C. rodentium
IF Bacteria
Peters EB, Tsihlis ND, Karver MR et al. Atheroma Niche-Responsive Nanocarriers for Immunotherapeutic Delivery. Adv Healthc Mater Jan 8 2019 [PMID: 30620448] (Mouse) Mouse
Ho CF, Ismail NB, Koh JK et al. Localisation of Formyl-Peptide Receptor 2 in the Rat Central Nervous System and Its Role in Axonal and Dendritic Outgrowth Neurochem. Res. Jun 13 2018 [PMID: 29948727] (WB, Rat) WB Rat
Motwani MP, Colas RA, George MJ et al. Pro-resolving mediators promote resolution in a human skin model of UV-killed Escherichia coli-driven acute inflammation. JCI Insight. Mar 22 2018 [PMID: 29563331] (IHC-P, Human) IHC-P Human
Horibe K, Hosoya A, Hiraga T, Nakamura H. Expression and localization of CRAMP in rat tooth germ and during reparative dentin formation. Clin Oral Investig. 2018 Feb 02 [PMID: 29396644] (IHC, Rat) IHC Rat
Show All 20 Publications.

Reviews for FPRL1/FPR2 Antibody (NLS1878) (4) 4.54

Average Rating: 4.5
(Based on 4 reviews)
We have 4 reviews tested in 1 species: Mouse.

Reviews using NLS1878:
Filter by Applications
WB
(2)
ICC
(1)
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(4)
All Species
Images Ratings Applications Species Date Details
Western Blot FPRL1/FPR2 NLS1878
Enlarge
5
reviewed by:
Anonymous
WB Mouse 09/14/2019
View

Summary

ApplicationWestern Blot
Sample Testedmouse primary cell,macrophage cell lysate
SpeciesMouse
LotA-2
Immunocytochemistry FPRL1/FPR2 NLS1878
Enlarge
5
reviewed by:
Anonymous
ICC Mouse 08/10/2019
View

Summary

ApplicationImmunocytochemistry
Sample TestedJ774A.1 mouse reticulum cell sarcoma macrophage cell line
SpeciesMouse
LotA-2
Western Blot FPRL1/FPR2 NLS1878
Enlarge
4
reviewed by:
Eric Hamlett
WB Mouse 08/16/2018
View

Summary

ApplicationWestern Blot
Sample Tested Mouse brain
SpeciesMouse
LotFPRL1/FPR2 Antibody

Comments

CommentsHomogenates of Mouse Brain in RIPA Buffer
  4
reviewed by:
Anonymous
IHC-Fr Mouse 12/29/2014
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 23603910
SpeciesMouse
Special ApplicationsSee PMID 23603910

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FPRL1/FPR2 Antibody (NLS1878). (Showing 1 - 4 of 4 FAQs).

  1. I am looking for an FPRL1 antibody that is reactive to mouse only. Does Novus have the antibody in question?
    • To answer your question, unfortunately all our FPRL1 antibodies are polyclonal and would detect both mouse and human formyl peptide receptor-like-1 and we do not have any mouse monoclonal antibody for it.
  2. We would like to know the right target for this antibody.
    • Our antibody with catalogue number NLS1878 was raised against a synthetic peptide derived from the second extracellular loop of the human FPRL1 protein
  3. I am interested in using this antibody for immunohistochemical staining of formalin-fixed rat tissues. I would like to know if this antibody is labeled with any substrate that may aid immunohistochemical staining, or would the HRP Labeled (NLS1878H) or Biotin Labeled (NLS1878B) form be more suitable for my application of interest?
    • Our product NLS1878 is provided as an unlabeled primary antibody. In order to use this antibody for staining in FFPE tissues, you will need to use a directly conjugated anti-rabbit IgG secondary. If you would like to use a directly conjugated primary antibody like NLS1878H or NLS1878B, you will not have to use a secondary antibody for detection. Depending on which detection reagents you are using, that will determine if you need the HRP conjugated or Biotin conjugated antibodies.
  4. May I know if there is a blocking peptide available for this antibody?
    • We do have the blocking peptide available for this item. It is sold under product #NLS1878PEP: Blocking Peptide.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NLS1878AF405
Alexa Fluor 488 NLS1878AF488
Alexa Fluor 647 NLS1878AF647
Alexa Fluor 700 NLS1878AF700
Biotin NLS1878B
DyLight 405 NLS1878V
DyLight 488 NLS1878G
DyLight 550 NLS1878R
DyLight 650 NLS1878C
FITC NLS1878F
HRP NLS1878H

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Recent Reviews

4.5
5
2
4
2
3
0
2
0
1
0

Anonymous
09/14/2019
Application: WB
Species: Mouse

Anonymous
08/10/2019
Application: ICC
Species: Mouse

Eric Hamlett
08/16/2018
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol FPR2
Entrez
Uniprot