ZEB1 Antibody


Western Blot: ZEB1 Antibody [NBP2-13159] - WB analysis of Zeb1 in 1) HeLa 2) Cos7 3) HepG2 and 4) Ntera2 cell lysates.
Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody [NBP2-13159] - ZEB1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Simple Western: ZEB1 Antibody [NBP2-13159] - Simple Western lane view shows a specific band for ZEB1 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Reactivity Hu, PmSpecies Glossary
Applications WB, Simple Western, ICC/IF
1.00 mg/ml

Order Details

Available Conjugates

ZEB1 Antibody Summary

A synthetic peptide made to an C-terminal portion of the human ZEB1 protein (between residues 1087-1124). [UniProt P37275]
Nucleus and cytoplasm.
Mesenchymal Cells Marker
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 30% glycerol
0.01% Sodium Azide
1.00 mg/ml
Immunogen affinity purified


  • Western Blot 1:1000
  • Simple Western 1:200
  • Immunocytochemistry/Immunofluorescence 1:40
Application Notes
This ZEB1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot. In ICC/IF, staining was seen in the nucleus with some weak punctate cytoplasmic staining in HeLa cells. In Western Blot, a band is seen ~180 kDa in HeLa cell lysate, cos7, Ntera2, and HepG2 cell lysates.In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Reactivity Notes

Human and monkey.

Alternate Names for ZEB1 Antibody

  • AREB6
  • AREB6MGC133261
  • BZP
  • delta-crystallin enhancer binding factor 1
  • FECD6
  • Negative regulator of IL2
  • NIL-2-A zinc finger protein
  • NIL2A
  • NIL-2-A
  • posterior polymorphous corneal dystrophy 3
  • PPCD3
  • TCF8
  • TCF-8
  • transcription factor 8 (represses interleukin 2 expression)
  • Transcription factor 8
  • ZEB
  • ZFHX1A
  • zinc finger E-box binding homeobox 1
  • zinc finger E-box-binding homeobox 1
  • zinc finger homeodomain enhancer-binding protein


ZEB1, also known as AREB6, is a zinc finger transcription factor encoding protein that represses T-lymphocyte-specific interleukin-2 (IL-2) gene expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (PMID: 1840704). ZEB1 is expressed in both skeletal and heart muscle, but is not found in the liver. ZEB1 mutations can cause posterior polymorphous corneal dystrophy type 3 (PPCD3) as well as corneal dystrophy Fuchs endothelial type 6 (FECD6). The former is a condition characterized by the metaplasia and overgrowth of corneal endothelial cells thus leading to an increased risk for glaucoma. The latter ocular disorder, FECD6, leads to reduced vision and pain and is caused by loss of endothelium of the central cornea. ZEB1 antibodies are useful tools for studying certain degenerative ocular diseases.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for ZEB1 Antibody (NBP2-13159): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ZEB1 Antibody (NBP2-13159). (Showing 1 - 1 of 1 FAQ).

  1. In looking at the Zeb1 antibodies on your website, the molecular weights of Zeb1 detected by several antibodies are different. Why is this?
    • ZEB1 is a rather large protein and undergoes a lot of post translational modifications such as phosphorylation and glycosylation (please see: UniProt P37275). The reason band patterns are different in the images may be due to sample type and how highly modified the ZEB1 protein is that the antibody is detecting. The more post translational modifications the higher you will detect the protein.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

DyLight 405 Labeled NBP2-13159V
FITC Labeled NBP2-13159F

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Blogs on ZEB1.

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Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

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Contact Information

Product PDFs


Gene Symbol ZEB1

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