ZAG Antibody [Unconjugated]

Western blot shows lysates of human prostate tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ZAG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4764) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ZAG at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

ZAG was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human ZAG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4764) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

ZAG was detected in immersion fixed human mesenchymal stem cells differentiated into adipocytes using Goat Anti-Human ZAG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4764) at 10 µg/mL for 3 hours at room temperature. Cells were differentiated using using StemXVivo Osteogenic/Adipogenic Base Media (Catalog # CCM007) and StemXVivo Adipogenic Supplement (100X) (Catalog # CCM011). Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

ZAG Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ZAG
  Gln21-Ser298
  Accession # Q5XKQ4
• Specificity: Detects human ZAG in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AZGP1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ZAG Antibody [Unconjugated]

• alpha-2-glycoprotein 1, zinc
• alpha-2-glycoprotein 1, zinc-binding
• AZGP1
• ZA2G
• ZAG
• ZAGzn-alpha-2-GP
• zinc
• zinc-alpha-2-glycoprotein
• zn-alpha-2-glycoprotein
• ZNGP1

Background

ZAG (zinc-alpha ₂-glycoprotein; also ZA2G) is a 40 kDa, secreted member of the MHC class I family of proteins. It is produced by adipocytes and various epithelial cells that generate exocrine-type secretions. ZAG is reported to stimulate lipid breakdown and thus may play a role in lipid homeostasis. Mature human ZAG is 278 amino acids (aa) in length. It contains one MHC class I antigen region (aa 26‑201) and a C2‑type Ig‑like domain (aa 207‑292). Two alternate splice forms exist; one shows a 66 aa substitution for the C‑terminal 30 aa, and a second shows a nine Lys substitution for aa 151‑298. Mature human ZAG is 60% aa identical to mouse ZAG.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AZGP1
• Uniprot:
  • Q5XKQ4()