ZAG Antibody (842014) [Unconjugated]

ZAG was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human ZAG Monoclonal Antibody (Catalog # MAB47641) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

ZAG was detected in immersion fixed 3T3-L1 mouse embryonic fibroblast adipose-like cell line using Mouse Anti-Human ZAG Monoclonal Antibody (Catalog # MAB47641) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu
• Applications: ICC/IF
• Clone: 842014
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

ZAG Antibody (842014) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ZAG
  Met1-Ser298
  Accession # P25311
• Specificity: Detects human ZAG in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ZAG Antibody (842014) [Unconjugated]

• alpha-2-glycoprotein 1, zinc
• alpha-2-glycoprotein 1, zinc-binding
• AZGP1
• ZA2G
• ZAG
• ZAGzn-alpha-2-GP
• zinc
• zinc-alpha-2-glycoprotein
• zn-alpha-2-glycoprotein
• ZNGP1

Background

ZAG (zinc-alpha ₂-glycoprotein; also ZA2G) is a 40 kDa, secreted member of the MHC class I family of proteins. It is produced by adipocytes and various epithelial cells that generate exocrine-type secretions. ZAG is reported to stimulate lipid breakdown and thus may play a role in lipid homeostasis. Mature human ZAG is 278 amino acids (aa) in length. It contains one MHC class I antigen region (aa 26‑201) and a C2‑type Ig‑like domain (aa 207‑292). Two alternate splice forms exist; one shows a 66 aa substitution for the C‑terminal 30 aa, and a second shows a nine Lys substitution for aa 151‑298. Mature human ZAG is 60% aa identical to mouse ZAG.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P25311()