WT1 Antibody - BSA Free

Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314] - Mouse testis. Wilms Tumor 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314] - Mouse liver. Wilms Tumor 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314] - Rat testis. Wilms Tumor 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314] - Rat testis. Wilms Tumor 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: WT1 Antibody [NBP1-31314] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with wt1a + wt1b antibody (NBP1-31314) diluted at 1:1000.

• Reactivity: Mu, Rt, Ze, Hu, Po, Ch
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

WT1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit WT1 Antibody - BSA Free (NBP1-31314) is a polyclonal antibody validated for use in IHC. Anti-WT1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human WT1. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Human (100%), Porcine (97%), Chicken (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WT1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Japanese medaka (81%), Xenopus laevis (85%). Rat reactivity reported in scientific literature (PMID: 7926762).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for WT1 Antibody - BSA Free

• AWT1
• GUD
• NPHS4GUD
• WAGR
• Wilms tumor 1
• Wilms tumor protein
• WIT-2
• WT1
• WT33

Background

Wilm's Tumor (WT) is a 55 kDa protein thought to play a major role in various cancers and developmental disorders, commonly kidney tumors and glomerular diseases. WT is a zinc finger transcription factor that is mutated in Wilm's tumors and highly expressed in a wide variety of other malignancies (1). It is believed that WT in its two isoforms acts as both a transcription factor and a post-transcriptional regulator that binds RNA and can interact with splicing components (2). Mutations in the zinc finger regions of WT are detected in nearly all patients of Denys-Drash syndrome and in some patients with isolated diffuse mesangial sclerosis. Also, mutations leading to the loss of the transcription factor isoform have been observed in all patients with Frasier syndrome (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for WT1 Antibody (NBP1-31314)(2)

Publications using NBP1-31314

• Husain S, Ginawi I, Bashir AI et al. Focal and segmental glomerulosclerosis in murine models: a histological and ultrastructural characterization with immunohistochemistry correlation of glomerular CD44 and WT1 expression. Ultrastruct Pathol. 2018-10-04 [PMID: 30285525] (IHC-P, Rat)
• Lim Beom Jin, Yang Jae Won, Zou Jun et al. Tubulointerstitial fibrosis can sensitize the kidney to subsequent glomerular injury. Kidney International 2017-01-01 [PMID: 28709637] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: WT1