![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/1434R) [NBP2-53182] - Formalin-fixed, paraffin-embedded human Mesothelioma stained with WT1 Antibody (WT1/1434R).](http://images.novusbio.com/fullsize2/WT1-Antibody-(WT1-1434R)-Immunohistochemistry-Paraffin-NBP2-53182-img0002.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (WT1/1434R) [NBP2-53182] - Formalin-fixed, paraffin-embedded human Mesothelioma stained with WT1 Antibody (WT1/1434R).")
| Reactivity | HuSpecies Glossary |
| Applications | Flow, ICC/IF, IHC, IHC-P |
| Clone | WT1/1434R |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Recombinant human full-length WT1 protein |
| Localization | Nuclear & Cytoplasmic |
| Marker | Wilms Tumor & Mesothelial Marker |
| Protein/Peptide Type | Recombinant Protein |
| Isotype | IgG Kappa |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | WT1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IHC-P: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer pH 6.0 for 10-20 min followed by cooling at RT for 20 minutes. |
| Theoretical MW | 51 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Diseases for WT1 Antibody (NBP2-53182)Discover more about diseases related to WT1 Antibody (NBP2-53182).
| Pathways for WT1 Antibody (NBP2-53182)View related products by pathway.
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PTMs for WT1 Antibody (NBP2-53182)Learn more about PTMs related to WT1 Antibody (NBP2-53182).
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Routine WT1 Antibody Screen Uncovers an Exciting New Cancer-Cleaning Enzyme Enzyme antibodies are widely used in both apoptosis and cancer studies, as disruption of the proteins regulating apoptosis is known to lead to formation of tumor cells in certain cancers. We at Novus Biologicals are continually growing our enzyme anti... Read full blog post. |
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| Gene Symbol | WT1 |
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/1434R) [NBP2-53182] - Formalin-fixed, paraffin-embedded Human Kidney stained with Wilm's Tumor Recombinant Rabbit Monoclonal Antibody (WT1/1434R).](http://images.novusbio.com/fullsize2/WT1-Antibody-(WT1-1434R)-Immunohistochemistry-Paraffin-NBP2-53182-img0001.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (WT1/1434R) [NBP2-53182] - Formalin-fixed, paraffin-embedded Human Kidney stained with Wilm's Tumor Recombinant Rabbit Monoclonal Antibody (WT1/1434R).")
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/1434R) [NBP2-53182] - Formalin-fixed, paraffin-embedded Rat Testis stained with WT1 Antibody (WT1/1434R).](http://images.novusbio.com/fullsize2/WT1 Antibody (WT1-1434R)-Immunohistochemistry-Paraffin-NBP2-53182-img0003.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (WT1/1434R) [NBP2-53182] - Formalin-fixed, paraffin-embedded Rat Testis stained with WT1 Antibody (WT1/1434R).")
![Western Blot: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP1-52823] - Titration: 0.2-1 ug/ml, Positive Control: THP-1 cell lysate.](http://images.novusbio.com/images2/SF-1-NR5A1-Steroidogenic-Factor-1-Antibody-Western-Blot-NBP1-52823-img0007.jpg)
![Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP1-52823] - Human adrenal tissue at an antibody concentration of 4-8ug/ml.](http://images.novusbio.com/images2/SF-1-NR5A1-Steroidogenic-Factor-1-Antibody-Immunohistochemistry-Paraffin-NBP1-52823-img0006.jpg)
![Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Western-Blot-NB200-103-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Immunocytochemistry-Immunofluorescence-NB200-103-img0013.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Immunohistochemistry-Paraffin-NB200-103-img0005.jpg)
![Western Blot: EGR1 Antibody (6E8) [H00001958-M03] - EGR1 monoclonal antibody (M03), clone 6E8. Western Blot analysis of EGR1 expression in PC-12.](http://images.novusbio.com/images2/EGR1-Antibody-(6E8)-Western-Blot-H00001958-M03-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: EGR1 Antibody (6E8) [H00001958-M03] - Analysis of monoclonal antibody to EGR1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/images2/EGR1-Antibody-(6E8)-Immunocytochemistry-Immunofluorescence-H00001958-M03-img0010.jpg)
![Immunohistochemistry-Paraffin: EGR1 Antibody (6E8) [H00001958-M03] - Analysis of monoclonal antibody to EGR1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml](http://images.novusbio.com/images2/EGR1-Antibody-(6E8)-Immunohistochemistry-Paraffin-H00001958-M03-img0011.jpg)
![Western Blot: EWSR1 Antibody (5H7) [NBP1-92686] - Analysis of different cell line lysates using EWSR1 antibody, dilution 1:1000 (Green): [1] protein standard (Red), [2] HeLa, [3] HEK293, [4] mouse NIH-3T3, [5] rat PC12, [6] equine NBL6 and [7] canine A72 cells. The strong band at ~80kDa corresponds to the EWS protein seen in all species tested.](http://images.novusbio.com/images2/EWSR1-Antibody-(5H7)-Western-Blot-NBP1-92686-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: EWSR1 Antibody (5H7) [NBP1-92686] - HeLa cell cultures stained with antibody NBP1-92686 (green) and chicken antibody to vimentin (NB300-223, red). Blue is a DNA stain, and it is clear that in these cells EWSR1 is localized along with the DNA in the nucleus.](http://images.novusbio.com/images2/EWSR1 Antibody (5H7)-Immunocytochemistry-Immunofluorescence-NBP1-92686-img0003.jpg)
![Western Blot: Pax2 Antibody (3C7) [H00005076-M01] - Analysis of PAX2 expression in transfected 293T cell line by PAX2 monoclonal antibody (M01), clone 3C7.Lane 1: PAX2 transfected lysate(47.41 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/Pax2-Antibody-(3C7)-Western-Blot-H00005076-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: Pax2 Antibody (3C7) [H00005076-M01] - Analysis of monoclonal antibody to PAX2 on formalin-fixed paraffin-embedded human adrenal gland. Antibody concentration 0.5 ug/ml.](http://images.novusbio.com/images2/Pax2-Antibody-(3C7)-Immunohistochemistry-Paraffin-H00005076-M01-img0004.jpg)
![ELISA: Pax2 Antibody (3C7) [H00005076-M01] - Detection limit for recombinant GST tagged PAX2 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/images2/Pax2-Antibody-(3C7)-ELISA-H00005076-M01-img0007.jpg)
![Western Blot: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - WB analysis of lysates from (A) Human Embryonic Kidney 293 / HEK293 and (B) Human Embryonic carcinoma NCCIT cell lines using Flt-3/Flk-2/CD135 antibody (clone 7E8.2C8) at 2ug/ml concentration. The antibody detected single specific bands in both samples at the expected molecular weight position.](http://images.novusbio.com/images2/Flt-3-Flk-2-CD135-Antibody-(7E8.2C8)-Western-Blot-NBP2-42210-img0001.jpg)
![Immunohistochemistry-Paraffin: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - IHC analysis of a formalin fixed paraffin embedded tissue section of human liver cancer using Flt-3/Flk-2/CD135 antibody (clone 7E2.2C8) at 15 ug/ml concentration. The antibody generated specific cytoplasmic immunostaining of FLT3 in the cancerous cells while the tumor stroma was largely negative for the staining.](http://images.novusbio.com/images2/Flt-3-Flk-2-CD135-Antibody-(7E8.2C8)-Immunohistochemistry-Paraffin-NBP2-42210-img0003.jpg)
![Flow Cytometry: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - HEK293 cells were stained (surface) with Flt-3/Flk-2/CD135 antibody (clone: 7E8.2C8; red) or isotype control (mouse IgG2 lambda; blue) and positive staining observed using PE conjugated mouse anti-IgG(H+L) secondary antibody. Live cells (PPI negative) were gated for analysis](http://images.novusbio.com/images2/Flt-3-Flk-2-CD135-Antibody-(7E8.2C8)-Flow-Cytometry-NBP2-42210-img0002.jpg)
![Western Blot: SRY Antibody (1G4) [NBP2-37463] - Analysis using SRY mouse mAb against NTERA-2 (1) cell lysate.](http://images.novusbio.com/images2/SRY-Antibody-(1G4)-Western-Blot-NBP2-37463-img0003.jpg)
![Immunohistochemistry-Paraffin: SRY Antibody (1G4) [NBP2-37463] - Analysis of ovarian cancer tissues using SRY mouse mAb with DAB staining.](http://images.novusbio.com/images2/SRY-Antibody-(1G4)-Immunohistochemistry-NBP2-37463-img0006.jpg)
![Flow Cytometry: SRY Antibody (1G4) [NBP2-37463] - Analysis of HepG2 cells using SRY mouse mAb (green) and negative control (red).](http://images.novusbio.com/images2/SRY-Antibody-(1G4)-Flow-Cytometry-NBP2-37463-img0004.jpg)
![Western Blot: Nucleophosmin Antibody [NB110-61646] - Detection of mutant Nucleophosmin in OCI-AML3 lysates.](http://images.novusbio.com/images2/Nucleophosmin-Antibody-Western-Blot-NB110-61646-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleophosmin Antibody [NB110-61646] - Nucleophosmin localization by immunofluorescence in HL-60 cells (negative control).](http://images.novusbio.com/images2/Nucleophosmin-Antibody-Immunocytochemistry-Immunofluorescence-NB110-61646-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleophosmin Antibody [NB110-61646] - Nucleophosmin localization by immunofluorescence in OCI-AML3 cells.](http://images.novusbio.com/images2/Nucleophosmin-Antibody-Immunocytochemistry-Immunofluorescence-NB110-61646-img0010.jpg)
![Western Blot: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of NPHS2 on rat kidney (1) and human kidney (2) tissue lysate using anti-NPHS2 antibody at 1/500 dilution.](http://images.novusbio.com/images2/Podocin-NPHS2 Antibody (JB51-33)-Western Blot-NBP2-75624-img0005.jpg)
![Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of paraffin-embedded rat kidney tissue using anti-NPHS2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/Podocin-NPHS2-Antibody-(JB51-33)-Immunohistochemistry-Paraffin-NBP2-75624-img0004.jpg)
![Flow Cytometry: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of 293T cells with NPHS2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/images2/Podocin-NPHS2-Antibody-(JB51-33)-Flow-Cytometry-NBP2-75624-img0001.jpg)
![Western Blot: Vimentin Antibody [NB300-223] - Analysis of tissue and cell lysates. Antibody at 1:5000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH-3T3 cell lysates. NB300-223 binds to the vimentin protein showing a single band at ~50 kDa. The blot was simultaneously probed with mouse mAb to MAP2C/D, dilution 1:5000 in green, revealing multiple bands around 280 kDa that correspond to full length MAP2A/2B isotypes, and ~70 kDa bands which are MAP2C/D isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells.](http://images.novusbio.com/images2/Vimentin-Antibody-Western-Blot-NB300-223-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - Comparative analysis of epithelial and mesenchymal markers expressions. Fluorescence immunocytochemistry staining for E-cadherin (green), N-cadherin (red) and vimentin (cyan) was performed on esophageal cancer cells under static and microfluidic flow conditions at different time points. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep38221), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Vimentin Antibody-Immunocytochemistry-Immunofluorescence-NB300-223-img0008.jpg)
![Immunohistochemistry: Vimentin Antibody [NB300-223] - Increased numbers of differentiating NSPCs in SVZ of G93A-SOD1 brain. Confocal microscopy images of dorsal and ventral regions of SVZ in G93A-SOD1 and Wt-SOD1 brain at postnatal week 18, stained for nestin (green), GFAP (red), and vimentin (blue). Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (http://www.molecularbrain.com/content/8/1/5), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Vimentin-Antibody-Immunohistochemistry-NB300-223-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 antibody was tested in WEHI-3 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/images2/CD34 Antibody (MEC 14.7)-Immunocytochemistry-Immunofluorescence-NB600-1071-img0003.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of mouse small intestine using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using HRP-conjugated anti-rat secondary with DAB reagent which followed counterstaining of nuclei using hematoxylin. This antibody specifically labelled the endothelial cells in blood vessels located primarily in the sub-mucosa, and of that of the mucosa muscularis and the mucosal lacteal.](http://images.novusbio.com/images2/CD34-Antibody-(MEC-14.7)-Immunohistochemistry-Paraffin-NB600-1071-img0014.jpg)
![Flow Cytometry: CD34 Antibody (MEC 14.7) [NB600-1071] - An intracellular stain was performed on Raw264.7 cells with CD34 Antibody [MEC 14.7] NB600-1071 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 488 (SA5-10018, Thermo Fisher).](http://images.novusbio.com/images2/CD34-Antibody-(MEC-14.7)-Flow-Cytometry-NB600-1071-img0015.jpg)
![Western Blot: Exosome component 1 Antibody (1H9) [NBP2-45545] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Exosome component 1.](http://images.novusbio.com/images2/Exosome component 1 Antibody (1H9)-Western Blot-NBP2-45545-img0023.jpg)
![Immunohistochemistry: Exosome component 1 Antibody (1H9) [NBP2-45545] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/images2/Exosome-component-1-Antibody-(1H9)-Immunohistochemistry-NBP2-45545-img0022.jpg)
![Immunohistochemistry: Exosome component 1 Antibody (1H9) [NBP2-45545] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/images2/Exosome-component-1-Antibody-(1H9)-Immunohistochemistry-NBP2-45545-img0001.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of sheep retinal extract](http://images.novusbio.com/images2/Pax6-Antibody-Western-Blot-NB300-750-img0023.jpg)
![Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NB300-750] - ARPE-19 monolayer on glass substrate. Fixative Methanol Blocking Goat Serum. Dilution 1:100. Incubation 1h @ RT. This image was submitted via customer Review.](http://images.novusbio.com/images2/Pax6 Antibody-Immunocytochemistry-NB300-750-img0024.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of rat whole eye extract](http://images.novusbio.com/images2/Pax6-Antibody-Western-Blot-NB300-750-img0017.jpg)
