Wnt-1 Antibody - BSA Free

Western Blot: Wnt-1 Antibody [NBP1-77948] - This antibody was used at a 1:1,400 dilution in PBS containing 1% BLOTTO. The specificity of the antibody was confirmed by peptide competition which completely blocked reaction of the antibody with Wnt-1 (data not shown).

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Wnt-1 Antibody - BSA Free Summary

• Description: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This affinity purified Wnt-1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Wnt-1 protein. (Uniprot: P04628)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WNT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:25000
  • Western Blot 1:1500-1:6000
• Application Notes: This affinity purified antibody has been tested for use in ELISA and western blotting.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with Wnt-1 from mouse, human, rat, bovine, dog, macaque, and opossum based on a 100% homology with the immunizing sequence. Partial cross-reactivity is expected against chicken Wnt-1 based on a 91% sequence homology. Cross-reactivity with Wnt-1 from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Wnt-1 Antibody - BSA Free

• Int-1
• INT1Proto-oncogene Int-1 homolog
• proto-oncogene Wnt-1
• wingless-type MMTV integration site family, member 1 (oncogene INT1)
• wingless-type MMTV integration site family, member 1
• Wnt1
• Wnt-1

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt1 (Wingless-type MMTV integration site family member 1) is a member of the WNT gene family. It is highly conserved in evolution and the protein encoded by this gene is known to be 98% identical to mouse Wnt1 protein at the amino acid level. Studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. Wnt1 is secreted as an extracellular matrix protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WNT1
• Uniprot:
  • NP_005421(Human)
  • P04628(Human)