Recombinant Human Wnt-3a Protein

• Reactivity: Hu
• Applications: Bioactivity

Recombinant Human Wnt-3a Protein Summary

• Details of Functionality: Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED₅₀ for this effect is 5-25 ng/mL. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED₅₀ for this effect is <500 ng/mL. Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line.
  Optimal concentrations should be determined by each laboratory for each application.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Wnt-3a protein
  Ser19-Lys352
• Accession #: NP_149122
• N-terminal Sequence: Ser19
• Protein/Peptide Type: Recombinant Proteins
• Gene: WNT3A
• Purity: >75%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Theoretical MW: 37.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 40 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
• Purity: >75%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Wnt-3a Protein

• MGC119418
• MGC119419
• MGC119420
• protein Wnt-3a
• wingless-type MMTV integration site family, member 3A
• Wnt3a
• Wnt-3a

Background

Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes (1). Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta -catenin levels (2). As intracellular beta -catenin levels rise, beta -catenin binds to TCF/LEF transcription factors leading to expression of Wnt target genes (3). Endo-IWR 1 (Catalog # 3532, # PSM1324) is a cell-permeant small molecule inhibitor of Axin turnover that suppresses Wnt signal transduction by stabilizing the beta -catenin destruction complex (4). Wnt-3a is a 44 kDa secreted hydrophobic glycoprotein containing a conserved pattern of 24 cysteine residues (5). Wnt-3a has two N-linked glycosylation sites (Asn 87, Asn 298), and Ser 209 is modified with palmitoleic acid (6). Glycosylation and acylation are essential for efficient Wnt secretion and biological activity, respectively (6, 7). Human Wnt-3a shares 96% amino acid (aa) identity with mouse mouse, bovine and canine Wnt-3a, and 89%, 86% and 84% aa identity with chicken, Xenopus and zebrafish Wnt-3a, respectively. It also shares 87% aa identity with Wnt3. During embryonic development, Wnt-3a is necessary for proper development of the hippocampus, anterior-posterior patterning, somite development, and tailbud formation (9-12). Wnt-3a also promotes self-renewal of hematopoietic stem cells, neural stem cells, and embryonic stem cells (13, 14).

1. Willert, K. and Nusse, R. (2012) Cold Spring Harb. Perspect. Biol. 4:a007864.
2. MacDonald, B.T. and X. He (2012) Cold Spring Harb. Perspect. Biol. 4:a007880.
3. Korinek, V. et al. (1997) Science 275:1784.
4. Chen, B. et al. (2009) Nat. Chem. Biol. 5:100.
5. Smolich, B.D. et al. (1993) Mol. Biol. Cell 4:1267.
6. Takada, R. et al. (2006) Dev. Cell 11:791.
7. Komekado, H. (2007) Genes Cells 12:521.
8. Dunty Jr. W. C. et al. (2008) Development 135:85.
9. Ikeya, M. and S. Takada (2001) Mech. Dev. 103:27.
10. Lee, S. M. et al. (2000) Development 127:457.
11. Takada, S. et al. (1994) Genes Dev. 8:174.
12. Willert, K. et al. (2003) Nature 423:6938.
13. Kalani, M.Y. et al. (2008) Proc. Natl. Acad. Sci. USA 105:16970.
14. Ten Berge, D. et al. (2011) Nat. Cell Biol. 13:1070.

Publications for Wnt-3a (5036-WN)(166)

Publications using 5036-WN

• C Bonamy, E Sechet, A Amiot, A Alam, M Mourez, L Fraisse, PJ Sansonetti, B Sperandio Expression of the human antimicrobial peptide ?-defensin-1 is repressed by the EGFR-ERK-MYC axis in colonic epithelial cells Sci Rep, 2018;8(1):18043. 2018 [PMID: 30575780] (Bioassay, Human)
• BA Brown, GM Connolly, CEJ Mill, H Williams, GD Angelini, JL Johnson, SJ George Aging differentially modulates the Wnt pro-survival signalling pathways in vascular smooth muscle cells Aging Cell, 2018;0(0):e12844. 2018 [PMID: 30548452] (Bioassay, Mouse)
• J Li, C Narayanan, J Bian, D Sambo, T Brickler, W Zhang, S Chetty A transient DMSO treatment increases the differentiation potential of human pluripotent stem cells through the Rb family PLoS ONE, 2018;13(12):e0208110. 2018 [PMID: 30540809] (Bioassay, Human)
• T Kijima, H Nakagawa, M Shimonoson, PM Chandramou, T Hara, V Sahu, Y Kasagi, O Kikuchi, K Tanaka, V Giroux, AB Muir, KA Whelan, S Ohashi, S Naganuma, AJ Klein-Szan, Y Shinden, K Sasaki, I Omoto, Y Kita, M Muto, AJ Bass, JA Diehl, GG Ginsberg, Y Doki, M Mori, Y Uchikado, T Arigami, NG Avadhani, D Basu, AK Rustgi, S Natsugoe Three-Dimensional Organoids Reveal Therapy Resistance of Esophageal and Oropharyngeal Squamous Cell Carcinoma Cells Cell Mol Gastroenterol Hepatol, 2019;7(1):73-91. 2019 [PMID: 30510992] (Bioassay, Human)
• H Ida, T Akiyama, K Ishiguro, SK Goparaju, Y Nakatake, N Chikazawa-, S Sato, H Kimura, Y Yokoyama, M Nagino, MSH Ko, SBH Ko Establishment of a rapid and footprint-free protocol for differentiation of human embryonic stem cells into pancreatic endocrine cells with synthetic mRNAs encoding transcription factors Stem Cell Res Ther, 2018;9(1):277. 2018 [PMID: 30359326] (Bioassay, Human)
• S Yamada, Y Kubo, D Yamazaki, Y Sekino, Y Nomura, S Yoshida, Y Kanda Tributyltin Inhibits Neural Induction of Human Induced Pluripotent Stem Cells Sci Rep, 2018;8(1):12155. 2018 [PMID: 30108368] (Bioassay, Human)
• E Magro-Lope, C Palmer, J Manso, I Liste, A Zambrano Effects of lung and airway epithelial maturation cocktail on the structure of lung bud organoids Stem Cell Res Ther, 2018;9(1):186. 2018 [PMID: 29996941] (Bioassay, Human)
• MJ Workman, JP Gleeson, EJ Troisi, HQ Estrada, SJ Kerns, CD Hinojosa, GA Hamilton, SR Targan, CN Svendsen, RJ Barrett Enhanced Utilization of Induced Pluripotent Stem Cell-Derived Human Intestinal Organoids Using Microengineered Chips Cell Mol Gastroenterol Hepatol, 2018;5(4):669-677.e2. 2018 [PMID: 29930984] (Bioassay, Human)
• Y Zhao, C Wang, C Wang, X Hong, J Miao, Y Liao, L Zhou, Y Liu An essential role for Wnt/?-catenin signaling in mediating hypertensive heart disease Sci Rep, 2018;8(1):8996. 2018 [PMID: 29895976] (Bioassay, Rat)
• M Russo, S Lamba, A Lorenzato, A Sogari, G Corti, G Rospo, B Mussolin, M Montone, L Lazzari, S Arena, D Oddo, M Linnebache, A Sartore-Bi, F Pietranton, S Siena, F Di Nicolan, A Bardelli Reliance upon ancestral mutations is maintained in colorectal cancers that heterogeneously evolve during targeted therapies Nat Commun, 2018;9(1):2287. 2018 [PMID: 29895949] (Bioassay, Human)
• J Li, F Xing, F Chen, L He, KF So, Y Liu, J Xiao Functional 3D Human Liver Bud Assembled from MSC-Derived Multiple Liver Cell Lineages Cell Transplant, 2018;0(0):9636897187803. 2018 [PMID: 29895168] (Bioassay, Human)
• D Garnier, R Li, F Delbos, A Fourrier, C Collet, C Guguen-Gui, C Chesné, TH Nguyen Expansion of human primary hepatocytes in vitro through their amplification as liver progenitors in a 3D organoid system Sci Rep, 2018;8(1):8222. 2018 [PMID: 29844473] (Bioassay, Human)
• A Sarkar, A Mei, ACM Paquola, S Stern, C Bardy, JR Klug, S Kim, N Neshat, HJ Kim, M Ku, MN Shokhirev, DH Adamowicz, MC Marchetto, R Jappelli, JA Erwin, K Padmanabha, M Shtrahman, X Jin, FH Gage Efficient Generation of CA3 Neurons from Human Pluripotent Stem Cells Enables Modeling of Hippocampal Connectivity In�Vitro Cell Stem Cell, 2018;22(5):684-697.e9. 2018 [PMID: 29727680] (Bioassay, Human)
• BN Nakamura, A Glazier, MG Kattah, B Duong, Y Jia, D Campo, L Shao A20 regulates canonical wnt-signaling through an interaction with RIPK4 PLoS ONE, 2018;13(5):e0195893. 2018 [PMID: 29718933] (Bioassay, Human)
• HK Lee, EW Lee, J Seo, M Jeong, SH Lee, SY Kim, EH Jho, CH Choi, JY Chung, J Song Ubiquitylation and degradation of adenomatous polyposis coli by MKRN1 enhances Wnt/?-catenin signaling Oncogene, 2018;0(0):. 2018 [PMID: 29713058] (Bioassay, Human)
• M Amin, Y Kushida, S Wakao, M Kitada, K Tatsumi, M Dezawa Cardiotrophic Growth Factor-Driven Induction of Human Muse Cells Into Cardiomyocyte-Like Phenotype Cell Transplant, 2018;27(2):285-298. 2018 [PMID: 29637816] (Bioassay, Human)
• H He, F Du, Y He, Z Wei, C Meng, Y Xu, H Zhou, N Wang, XG Luo, W Ma, TC Zhang The Wnt-?-catenin signaling regulated MRTF-A transcription to activate migration-related genes in human breast cancer cells Oncotarget, 2018;9(20):15239-15251. 2018 [PMID: 29632640] (Bioassay, Human)
• XG Li, Z Wang, RQ Chen, HL Fu, CQ Gao, HC Yan, GX Xing, XQ Wang LGR5 and BMI1 Increase Pig Intestinal Epithelial Cell Proliferation by Stimulating WNT/?-Catenin Signaling Int J Mol Sci, 2018;19(4):. 2018 [PMID: 29601474] (Bioassay, Porcine)
• HJ Lee, J Li, RE Vickman, J Li, R Liu, A Durkes, BD Elzey, S Yue, X Liu, TL Ratliff, JX Cheng Cholesterol Esterification Inhibition Suppresses Prostate Cancer Metastasis by Impairing the Wnt/?-catenin Pathway Mol. Cancer Res., 2018;0(0):. 2018 [PMID: 29545473] (Bioassay, Human)
• S Condello, LE Sima, C Ivan, H Cardenas, GE Schiltz, RK Mishra, D Matei Tissue transglutaminase regulates interactions between ovarian cancer stem cells and the tumor niche Cancer Res., 2018;0(0):. 2018 [PMID: 29510995] (Bioassay, Human)
• M Szemes, A Greenhough, Z Melegh, S Malik, A Yuksel, D Catchpoole, K Gallacher, M Kollareddy, JH Park, K Malik Wnt Signalling Drives Context-Dependent Differentiation or Proliferation in Neuroblastoma Neoplasia, 2018;20(4):335-350. 2018 [PMID: 29505958] (Bioassay, Human)
• S Kokabu, V Rosen BMP3 expression by osteoblast lineage cells is regulated by canonical Wnt signaling FEBS Open Bio, 2018;8(2):168-176. 2018 [PMID: 29435407] (Bioassay, Mouse)
• X Wang, M Sterr, I Burtscher, S Chen, A Hieronimus, F Machicao, H Staiger, HU Häring, G Lederer, T Meitinger, FM Cernilogar, G Schotta, M Irmler, J Beckers, M Hrab? de A, M Ray, CVE Wright, M Bakhti, H Lickert Genome-wide analysis of PDX1 target genes in human pancreatic progenitors Mol Metab, 2018;0(0):. 2018 [PMID: 29396371] (Bioassay, Human)
• Y Sui, Z Liu, SH Park, SE Thatcher, B Zhu, JP Fernandez, H Molina, PA Kern, C Zhou IKK? is a ?-catenin kinase that regulates mesenchymal stem cell differentiation JCI Insight, 2018;3(2):. 2018 [PMID: 29367460] (Bioassay, Mouse)
• Q Li, Q Wang, O Wang, K Shao, H Lin, Y Lei A simple and scalable hydrogel-based system for culturing protein-producing cells PLoS ONE, 2018;13(1):e0190364. 2018 [PMID: 29293594] (Bioassay, Human)
• DS Varghese, S Parween, MT Ardah, BS Emerald, SA Ansari Effects of Aminoglycoside Antibiotics on Human Embryonic Stem Cell Viability during Differentiation In Vitro Stem Cells Int, 2017;2017(0):2451927. 2017 [PMID: 29147115] (Bioassay, Human)
• V Uribe-Etxe, J Luzuriaga, P García-Gal, A Agliano, F Unda, G Ibarretxe Notch/Wnt cross-signalling regulates stemness of dental pulp stem cells through expression of neural crest and core pluripotency factors Eur Cell Mater, 2017;34(0):249-270. 2017 [PMID: 29092089] (Bioassay, Human)
• SJ Zhao, YQ Jiang, NW Xu, Q Li, Q Zhang, SY Wang, J Li, YH Wang, YL Zhang, SH Jiang, YJ Wang, YJ Huang, XX Zhang, GA Tian, CC Zhang, YY Lv, M Dai, F Liu, R Zhang, D Zhou, ZG Zhang SPARCL1 suppresses osteosarcoma metastasis and recruits macrophages by activation of canonical WNT/?-catenin signaling through stabilization of the WNT-receptor complex Oncogene, 2017;0(0):. 2017 [PMID: 29084211] (Bioassay, Human)
• H Xiao, Y Zeng, Q Wang, S Wei, X Zhu A Novel Positive Feedback Loop Between NTSR1 and Wnt/?-Catenin Contributes to Tumor Growth of Glioblastoma Cell. Physiol. Biochem., 2017;43(5):2133-2142. 2017 [PMID: 29065410] (cell culture, Human)
• S Miura, A Suzuki Generation of Mouse and Human Organoid-Forming Intestinal Progenitor Cells by Direct Lineage Reprogramming Cell Stem Cell, 2017;0(0):. 2017 [PMID: 28943029] (Bioassay, Human)
• N Yucer, M Holzapfel, T Jenkins Vo, L Lenaeus, L Ornelas, A Laury, D Sareen, R Barrett, BY Karlan, CN Svendsen Directed Differentiation of Human Induced Pluripotent Stem Cells into Fallopian Tube Epithelium Sci Rep, 2017;7(1):10741. 2017 [PMID: 28878359] (Bioassay, Human)
• X Li, C Wang, G Zhang, M Liang, B Zhang AKAP2 is upregulated in ovarian cancer, and promotes growth and migration of cancer cells Mol Med Rep, 2017;0(0):. 2017 [PMID: 28849175] (Bioassay, Human)
• S Premarathn, M Murtaza, N Matigian, LA Jolly, SA Wood Loss of Usp9x disrupts cell adhesion, and components of the Wnt and Notch signaling pathways in neural progenitors Sci Rep, 2017;7(1):8109. 2017 [PMID: 28808228] (Bioassay, Human)
• Y Kishimoto, B Ohkawara, T Sakai, M Ito, A Masuda, N Ishiguro, C Shukunami, D Docheva, K Ohno Wnt/?-catenin signaling suppresses expressions of Scx, Mkx, and Tnmd in tendon-derived cells PLoS ONE, 2017;12(7):e0182051. 2017 [PMID: 28750046] (Bioassay, Human)
• S Monteagudo, FMF Cornelis, C Aznar-Lope, P Yibmantasi, LA Guns, P Carmeliet, F Cailotto, RJ Lories DOT1L safeguards cartilage homeostasis and protects against osteoarthritis Nat Commun, 2017;8(0):15889. 2017 [PMID: 28627522] (Bioassay, Human)
• JG Camp, K Sekine, T Gerber, H Loeffler-W, H Binder, M Gac, S Kanton, J Kageyama, G Damm, D Seehofer, L Belicova, M Bickle, R Barsacchi, R Okuda, E Yoshizawa, M Kimura, H Ayabe, H Taniguchi, T Takebe, B Treutlein Multilineage communication regulates human liver bud development from pluripotency Nature, 2017;0(0):. 2017 [PMID: 28614297] (Bioassay, Human)
• S Zeng, AM Seifert, JQ Zhang, MJ Cavnar, TS Kim, VP Balachandr, JA Santamaria, NA Cohen, MJ Beckman, B Medina, F Rossi, MH Crawley, JK Loo, JH Maltbaek, P Besmer, CR Antonescu, RP DeMatteo Wnt/?-catenin signaling contributes to tumor malignancy and is targetable in gastrointestinal stromal tumor Mol. Cancer Ther., 2017;0(0):. 2017 [PMID: 28611108] (Bioassay, Human)
• ML Xu, CWC Bi, EYL Liu, TTX Dong, KWK Tsim Wnt3a Induces the Expression of Acetylcholinesterase during Osteoblast Differentiation via the Runx2 Transcription Factor J. Biol. Chem., 2017;0(0):. 2017 [PMID: 28607150] (Bioassay, Rat)
• H Isshiki, Y Arimura, K Nagaishi, K Kawakami, K Onodera, K Yamashita, Y Naishiro, M Fujimiya, K Imai, Y Shinomura Establishment of a refined culture method for rat colon organoids Biochem. Biophys. Res. Commun., 2017;0(0):. 2017 [PMID: 28559141] (Bioassay, Rat)
• XH Jiang, YT Xie, YP Cai, J Ren, T Ma Effects of hepatitis C virus core protein and nonstructural protein 4B on the Wnt/?-catenin pathway BMC Microbiol., 2017;17(1):124. 2017 [PMID: 28545480] (Bioassay, Human)
• B Lucendo-Vi, P Filis, MJ Swortwood, MA Huestis, J Meseguer-R, K Cameron, JP Iredale, PJ O'Shaughne, PA Fowler, DC Hay Modelling foetal exposure to maternal smoking using hepatoblasts from pluripotent stem cells Arch. Toxicol., 2017;0(0):. 2017 [PMID: 28510779] (Bioassay, Human)
• Q Gu, J Wang, L Wang, ZX Liu, WW Zhu, YQ Tan, WF Han, J Wu, CJ Feng, JH Fang, L Liu, L Wang, W Li, XY Zhao, BY Hu, J Hao, Q Zhou Accreditation of Biosafe Clinical-Grade Human Embryonic Stem Cells According to Chinese Regulations Stem Cell Reports, 2017;0(0):. 2017 [PMID: 28506532] (Bioassay, Human)
• I Savvidou, T Khong, A Cuddihy, C McLean, S Horrigan, A Spencer Beta-catenin inhibitor BC2059 is efficacious as monotherapy or in combination with proteasome inhibitor bortezomib in multiple myeloma Mol. Cancer Ther., 2017;0(0):. 2017 [PMID: 28500235] (Bioassay, Human)
• PC Sanchez-Di, JC Chang, ES Moses, T Dao, Y Chen, JY Hung Ubiquitin carboxyl-terminal esterase L1 (UCHL1) is associated with stem-like cancer cell functions in pediatric high-grade glioma PLoS ONE, 2017;12(5):e0176879. 2017 [PMID: 28472177] (Bioassay, Human)
• CY Janda, LT Dang, C You, J Chang, W de Lau, ZA Zhong, KS Yan, O Marecic, D Siepe, X Li, JD Moody, BO Williams, H Clevers, J Piehler, D Baker, CJ Kuo, KC Garcia Surrogate Wnt agonists that phenocopy canonical Wnt and ?-catenin signalling Nature, 2017;0(0):. 2017 [PMID: 28467818] (Bioassay, Human)
• K Low, LY Wong, M Maldonado, C Manjunath, CB Horner, M Perez, NV Myung, J Nam Physico-electrochemical Characterization of Pluripotent Stem Cells during Self-Renewal or Differentiation by a Multi-modal Monitoring System Stem Cell Reports, 2017;0(0):. 2017 [PMID: 28457888] (Bioassay, Human)
• JA Heslop, R Kia, CS Pridgeon, RL Sison-Youn, T Liloglou, M Elmasry, SW Fenwick, JS Mills, NR Kitteringh, CE Goldring, BK Park Donor-Dependent and Other Nondefined Factors Have Greater Influence on the Hepatic Phenotype Than the Starting Cell Type in Induced Pluripotent Stem Cell Derived Hepatocyte-Like Cells Stem Cells Transl Med, 2017;6(5):1321-1331. 2017 [PMID: 28456008] (Bioassay, Human)
• T Nakamura, T Hosoyama, J Murakami, M Samura, K Ueno, H Kurazumi, R Suzuki, A Mikamo, K Hamano Age-related increase in Wnt inhibitor causes a senescence-like phenotype in human cardiac stem cells Biochem. Biophys. Res. Commun., 2017;0(0):. 2017 [PMID: 28435069] (Bioassay, Human)
• F Ou, K Su, J Sun, W Liao, Y Yao, Y Zheng, Z Zhang The LncRNA ZBED3-AS1 induces chondrogenesis of human synovial fluid mesenchymal stem cells Biochem. Biophys. Res. Commun., 2017;487(2):457-463. 2017 [PMID: 28431932] (Bioassay, Human)
• JJ Kathiriya, N Nakra, J Nixon, PS Patel, V Vaghasiya, A Alhassani, Z Tian, D Allen-Gips, V Davé Galectin-1 inhibition attenuates profibrotic signaling in hypoxia-induced pulmonary fibrosis Cell Death Discov, 2017;3(0):17010. 2017 [PMID: 28417017] (Bioassay, Human)
• LA Ahrens, D Vonwil, J Christense, VP Shastri Gelatin device for the delivery of growth factors involved in endochondral ossification PLoS ONE, 2017;12(4):e0175095. 2017 [PMID: 28380024] (Bioassay, Human)
• H Montacir, N Freyer, F Knoespel, T Urbaniak, T Dedova, M Berger, G Damm, R Tauber, K Zeilinger, V Blanchard The N-glycome of embryonic stem cells and thereof differentiated hepatic cells Chembiochem, 2017;0(0):. 2017 [PMID: 28370937] (Bioassay, Human)
• DM Velázquez, MC Castañeda-, M Robles-Flo Dishevelled stability is positively regulated by PKC?-mediated phosphorylation induced by Wnt agonists Cell. Signal., 2017;35(0):107-117. 2017 [PMID: 28366812] (Bioassay, Human)
• KB McCauley, F Hawkins, M Serra, DC Thomas, A Jacob, DN Kotton Efficient Derivation of Functional Human Airway Epithelium from Pluripotent Stem Cells via Temporal Regulation of Wnt Signaling Cell Stem Cell, 2017;0(0):. 2017 [PMID: 28366587] (Bioassay, Human)
• J Messéant, J Ezan, P Delers, K Glebov, C Marchiol, F Lager, G Renault, F Tissir, M Montcouqui, N Sans, C Legay, L Strochlic Wnts contribute to neuromuscular junction formation through distinct signaling pathways Development, 2017;0(0):. 2017 [PMID: 28348167] (Bioassay, Mouse)
• K Minegishi, M Hashimoto, R Ajima, K Takaoka, K Shinohara, Y Ikawa, H Nishimura, AP McMahon, K Willert, Y Okada, H Sasaki, D Shi, T Fujimori, T Ohtsuka, Y Igarashi, TP Yamaguchi, A Shimono, H Shiratori, H Hamada A Wnt5 Activity Asymmetry and Intercellular Signaling via PCP Proteins Polarize Node Cells for Left-Right Symmetry Breaking Dev. Cell, 2017;40(5):439-452.e4. 2017 [PMID: 28292423] (Bioassay, Mouse)
• A Asai, E Aihara, C Watson, R Mourya, T Mizuochi, P Shivakumar, K Phelan, C Mayhew, M Helmrath, T Takebe, J Wells, JA Bezerra Paracrine signals regulate human liver organoid maturation from induced pluripotent stem cells Development, 2017;144(6):1056-1064. 2017 [PMID: 28275009] (Bioassay, Human)
• J Yang, Y Wang, T Zhou, LY Wong, XY Tian, X Hong, WH Lai, KW Au, R Wei, Y Liu, LH Cheng, G Liang, Z Huang, W Fan, P Zhao, X Wang, DP Ibañez, Z Luo, Y Li, X Zhong, S Chen, D Wang, L Li, L Lai, B Qin, X Bao, AP Hutchins, CW Siu, Y Huang, MA Esteban, HF Tse Generation of Human Liver Chimeric Mice with Hepatocytes from Familial Hypercholesterolemia Induced Pluripotent Stem Cells Stem Cell Reports, 2017;0(0):. 2017 [PMID: 28262545] (Bioassay, Human)
• A Persad, G Venkateswa, L Hao, ME Garcia, J Yoon, J Sidhu, S Persad Active ?-catenin is regulated by the PTEN/PI3 kinase pathway: a role for protein phosphatase PP2A Genes Cancer, 2016;7(12):368-382. 2016 [PMID: 28191283] (Bioassay, Human)
• J Jang, Y Jung, S Chae, SI Chung, SM Kim, Y Yoon WNT/?-catenin pathway modulates the TNF-?-induced inflammatory response in bronchial epithelial cells Biochem. Biophys. Res. Commun, 2017;0(0):. 2017 [PMID: 28137581] (Bioassay, Human)
• Z Wen, T Pan, S Yang, J Liu, H Tao, Y Zhao, D Xu, W Shao, J Wu, X Liu, Y Wang, J Mao, Y Zhu Up-regulated NRIP2 in colorectal cancer initiating cells modulates the Wnt pathway by targeting ROR? Mol. Cancer, 2017;16(1):20. 2017 [PMID: 28137278] (Bioassay, Human)
• J Jang, Y Jung, Y Kim, EH Jho, Y Yoon LPS-induced inflammatory response is suppressed by Wnt inhibitors, Dickkopf-1 and LGK974 Sci Rep, 2017;7(0):41612. 2017 [PMID: 28128299] (Bioassay, Human)
• Y Choudhury, YC Toh, J Xing, Y Qu, J Poh, L Huan, HS Tan, R Kanesvaran, H Yu, MH Tan Patient-specific hepatocyte-like cells derived from induced pluripotent stem cells model pazopanib-mediated hepatotoxicity Sci Rep, 2017;7(0):41238. 2017 [PMID: 28120901] (Bioassay, Human)
• IT Cho, Y Lim, JA Golden, G Cho Aristaless Related Homeobox (ARX) Interacts with ?-Catenin, BCL9, and P300 to Regulate Canonical Wnt Signaling PLoS ONE, 2017;12(1):e0170282. 2017 [PMID: 28103279] (Bioassay, Human)
• L J Spicer G protein-coupled receptor 34 in ovarian granulosa cells of cattle: changes during follicular development and potential functional implications Domest. Anim. Endocrinol, 2016;59(0):90-99. 2016 [PMID: 28040605] (Bioassay, Bovine)
• Aberrantly expressed LGR4 empowers Wnt signaling in multiple myeloma by hijacking osteoblast-derived R-spondins Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):. 2016 [PMID: 28028233] (Bioassay, Human)
• Hoeke A Baarsma Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD J. Exp. Med, 2016;0(0):. 2016 [PMID: 27979969] (Bioassay, Mouse)
• Gui-Li Huang Oncogenic retinoic acid receptor ? promotes human colorectal cancer growth through simultaneously regulating p21 transcription and GSK3?/?-catenin signaling Cancer Lett, 2016;388(0):118-129. 2016 [PMID: 27932034] (Bioassay, Human)
• Elena Neumann Expression of adipokines in osteoarthritis osteophytes and their effect on osteoblasts Matrix Biol., 2016;0(0):. 2016 [PMID: 27884778] (Bioassay, Human)
• Quantifying ?-catenin subcellular dynamics and cyclin D1 mRNA transcription during Wnt signaling in single living cells Elife, 2016;5(0):. 2016 [PMID: 27879202] (Bioassay, Mouse)
• Ronald A DePinho Epigenetic Activation of WNT5A Drives Glioblastoma Stem Cell Differentiation and Invasive Growth Cell, 2016;167(5):1281-1295.e18. 2016 [PMID: 27863244] (Bioassay, Human)
• Richard P Phipps The Aryl Hydrocarbon Receptor and Its Ligands Inhibit Myofibroblast Formation and Activation: Implications for Thyroid Eye Disease Am. J. Pathol., 2016;0(0):. 2016 [PMID: 27842700] (Bioassay, Human)
• PGE2 is a direct and robust mediator of anion/fluid secretion by human intestinal epithelial cells Sci Rep, 2016;6(0):36795. 2016 [PMID: 27827428] (Bioassay, Human)
• Karthikeyan Mythreye Altering the Proteoglycan State of Transforming Growth Factor ? Type III Receptor (T?RIII)/Betaglycan Modulates Canonical Wnt/?-Catenin Signaling J. Biol. Chem., 2016;291(49):25716-25728. 2016 [PMID: 27784788] (Bioassay, Human)
• ROCK inhibitor primes human induced pluripotent stem cells to selectively differentiate towards mesendodermal lineage via epithelial-mesenchymal transition-like modulation Stem Cell Res, 2016;17(2):222-227. 2016 [PMID: 27591478] (Bioassay, Human)
• Wnt signaling promotes breast cancer by blocking ITCH-mediated degradation of the YAP/TAZ transcriptional coactivator WBP2 Cancer Res, 2016;0(0):. 2016 [PMID: 27578003] (Bioassay, Human)
• TNIK inhibition abrogates colorectal cancer stemness Nat Commun, 2016;7(0):12586. 2016 [PMID: 27562646] (Bioassay, Human)
• Anthelmintic niclosamide disrupts the interplay of p65 and FOXM1/?-catenin and eradicates leukemia stem cells in chronic myelogenous leukemia Clin Cancer Res, 2016;0(0):. 2016 [PMID: 27492973] (Bioassay, Human)
• The AKAP GSKIP regulates beta-catenin through both its interactions with PKA and GSK3beta J Biol Chem, 2016;0(0):. 2016 [PMID: 27484798] (Bioassay, Human)
• Growth hormone is permissive for neoplastic colon growth Proc Natl Acad Sci USA, 2016;0(0):. 2016 [PMID: 27226307] (Bioassay, Human)
• Activation of endothelial ?-catenin signaling induces heart failure Sci Rep, 2016;6(0):25009. 2016 [PMID: 27146149] (Bioassay, Human)
• Modeling Smith-Lemli-Opitz syndrome with induced pluripotent stem cells reveals a causal role for Wnt/?-catenin defects in neuronal cholesterol synthesis phenotypes Nat. Med., 2016;22(4):388-96. 2016 [PMID: 26998835] (Bioassay, Human)
• Yasuhiro Ikeda Tumor-Free Transplantation of Patient-Derived Induced Pluripotent Stem Cell Progeny for Customized Islet Regeneration Stem Cells Transl Med, 2016;5(5):694-702. 2016 [PMID: 26987352] (Bioassay, Human)
• Tumor-Free Transplantation of Patient-Derived Induced Pluripotent Stem Cell Progeny for Customized Islet Regeneration Stem Cells Transl Med, 2016;5(5):694-702. 2016 [PMID: 26987352] (Bioassay, Human)
• Henry C, Llamosas E, Knipprath-Meszaros A, Schoetzau A, Obermann E, Fuenfschilling M, Caduff R, Fink D, Hacker N, Ward R, Heinzelmann-Schwarz V, Ford C Targeting the ROR1 and ROR2 receptors in epithelial ovarian cancer inhibits cell migration and invasion. Oncotarget, 0;6(37):40310-26. 0 [PMID: 26515598] (Bioassay, Human)
• Tamminen K, Balboa D, Toivonen S, Pakarinen M, Wiener Z, Alitalo K, Otonkoski T Intestinal Commitment and Maturation of Human Pluripotent Stem Cells Is Independent of Exogenous FGF4 and R-spondin1. PLoS ONE, 2015;10(7):e0134551. 2015 [PMID: 26230325] (Bioassay, Human)
• Zanni R, Galvez-Llompart M, Morell C, Rodriguez-Henche N, Diaz-Laviada I, Recio-Iglesias M, Garcia-Domenech R, Galvez J Novel cancer chemotherapy hits by molecular topology: dual Akt and Beta-catenin inhibitors. PLoS ONE, 2015;10(4):e0124244. 2015 [PMID: 25910265] (Bioassay, Human)
• Chang B, Tessneer K, McManus J, Liu X, Hahn S, Pasula S, Wu H, Song H, Chen Y, Cai X, Dong Y, Brophy M, Rahman R, Ma J, Xia L, Chen H Epsin is required for Dishevelled stability and Wnt signalling activation in colon cancer development. Nat Commun, 2015;6(0):6380. 2015 [PMID: 25871009] (Bioassay, Human)
• Tesfay L, Clausen K, Kim J, Hegde P, Wang X, Miller L, Deng Z, Blanchette N, Arvedson T, Miranti C, Babitt J, Lin H, Peehl D, Torti F, Torti S Hepcidin regulation in prostate and its disruption in prostate cancer. Cancer Res, 2015;75(11):2254-63. 2015 [PMID: 25858146] (Bioassay, Human)
• Maubant S, Tesson B, Maire V, Ye M, Rigaill G, Gentien D, Cruzalegui F, Tucker G, Roman-Roman S, Dubois T Transcriptome analysis of wnt3a-treated triple-negative breast cancer cells. PLoS ONE, 2015;10(4):e0122333. 2015 [PMID: 25848952] (Bioassay, Human)
• Iyer D, Gambardella L, Bernard W, Serrano F, Mascetti V, Pedersen R, Talasila A, Sinha S Robust derivation of epicardium and its differentiated smooth muscle cell progeny from human pluripotent stem cells. Development, 2015;142(8):1528-41. 2015 [PMID: 25813541] (Bioassay, Human)
• Kuhnl A, Valk P, Sanders M, Ivey A, Hills R, Mills K, Gale R, Kaiser M, Dillon R, Joannides M, Gilkes A, Haferlach T, Schnittger S, Duprez E, Linch D, Delwel R, Lowenberg B, Baldus C, Solomon E, Burnett A, Grimwade D Downregulation of the Wnt inhibitor CXXC5 predicts a better prognosis in acute myeloid leukemia. Blood, 2015;125(19):2985-94. 2015 [PMID: 25805812] (Bioassay, Human)
• Pezzolla D, Lopez-Beas J, Lachaud C, Dominguez-Rodriguez A, Smani T, Hmadcha A, Soria B Resveratrol ameliorates the maturation process of beta-cell-like cells obtained from an optimized differentiation protocol of human embryonic stem cells. PLoS ONE, 2015;10(3):e0119904. 2015 [PMID: 25774684] (Bioassay, Human)
• Kim M, Horst A, Blinka S, Stamm K, Mahnke D, Schuman J, Gundry R, Tomita-Mitchell A, Lough J Activin-A and Bmp4 levels modulate cell type specification during CHIR-induced cardiomyogenesis. PLoS ONE, 2015;10(2):e0118670. 2015 [PMID: 25706534] (Bioassay, Human)
• Tsankov, Alexande, Gu, Hongcang, Akopian, Veronika, Ziller, Michael, Donaghey, Julie, Amit, Ido, Gnirke, Andreas, Meissner, Alexande Transcription factor binding dynamics during human ES cell differentiation. Nature, 2015;518(7539):344-9. 2015 [PMID: 25693565] (Bioassay, Human)
• James M, Howells L, Karmokar A, Higgins J, Greaves P, Cai H, Dennison A, Metcalfe M, Garcea G, Lloyd D, Berry D, Steward W, Brown K Characterization and propagation of tumor initiating cells derived from colorectal liver metastases: trials, tribulations and a cautionary note. PLoS ONE, 2015;10(2):e0117776. 2015 [PMID: 25658706] (Bioassay, Human)
• Huang S, Green M, de Carvalho A, Mumau M, Chen Y, D'Souza S, Snoeck H The in vitro generation of lung and airway progenitor cells from human pluripotent stem cells. Nat Protoc, 2015;10(3):413-25. 2015 [PMID: 25654758] (Bioassay, Human)
• Wong, Amy P, Chin, Stephani, Xia, Sunny, Garner, Jodi, Bear, Christin, Rossant, Janet Efficient generation of functional CFTR-expressing airway epithelial cells from human pluripotent stem cells. Nat Protoc, 2015;10(3):363-81. 2015 [PMID: 25654755] (Bioassay, Human)
• Peng H, Park J, Katsnelson J, Kaplan N, Yang W, Getsios S, Lavker R microRNA-103/107 Family Regulates Multiple Epithelial Stem Cell Characteristics. Stem Cells, 2015;33(5):1642-56. 2015 [PMID: 25639731]
• Xue J, Chen Y, Wu Y, Wang Z, Zhou A, Zhang S, Lin K, Aldape K, Majumder S, Lu Z, Huang S Tumour suppressor TRIM33 targets nuclear beta-catenin degradation. Nat Commun, 2015;6(0):6156. 2015 [PMID: 25639486] (Bioassay, Human)
• Grigson E, Ozerova M, Pisklakova A, Liu H, Sullivan D, Nefedova Y Canonical Wnt pathway inhibitor ICG-001 induces cytotoxicity of multiple myeloma cells in Wnt-independent manner. PLoS ONE, 2015;10(1):e0117693. 2015 [PMID: 25635944] (Bioassay, Human)
• Huang T, Li L, Moalim-Nour L, Jia D, Bai J, Yao Z, Bennett S, Figeys D, Wang L A Regulatory Network Involving beta-Catenin, e-Cadherin, PI3k/Akt, and Slug Balances Self-Renewal and Differentiation of Human Pluripotent Stem Cells In Response to Wnt Signaling. Stem Cells, 2015;33(5):1419-33. 2015 [PMID: 25538040] (Bioassay, Human)
• Viale-Bouroncle S, Klingelhoffer C, Ettl T, Reichert T, Morsczeck C A protein kinase A (PKA)/beta-catenin pathway sustains the BMP2/DLX3-induced osteogenic differentiation in dental follicle cells (DFCs). Cell Signal, 2015;27(3):598-605. 2015 [PMID: 25530217] (Bioassay, Human)
• Handorf A, Chamberlain C, Li W Endogenously produced Indian Hedgehog regulates TGFbeta-driven chondrogenesis of human bone marrow stromal/stem cells. Stem Cells Dev, 2015;24(8):995-1007. 2015 [PMID: 25519748] (Bioassay, Human)
• Sundaram S, One J, Siewert J, Teodosescu S, Zhao L, Dimitrievska S, Qian H, Huang A, Niklason L Tissue-engineered vascular grafts created from human induced pluripotent stem cells. Stem Cells Transl Med, 2014;3(12):1535-43. 2014 [PMID: 25378654] (Bioassay, Human)
• Hirata, Naoya, Yamada, Shigeru, Shoda, Takuji, Kurihara, Masaaki, Sekino, Yuko, Kanda, Yasunari Sphingosine-1-phosphate promotes expansion of cancer stem cells via S1PR3 by a ligand-independent Notch activation. Nat Commun, 2014;5(0):4806. 2014 [PMID: 25254944] (Bioassay, Human)
• Jabaji Z, Brinkley G, Khalil H, Sears C, Lei N, Lewis M, Stelzner M, Martin M, Dunn J Type I collagen as an extracellular matrix for the in vitro growth of human small intestinal epithelium. PLoS ONE, 2014;9(9):e107814. 2014 [PMID: 25222024] (Bioassay, Human)
• Williams K, Bundred N, Landberg G, Clarke R, Farnie G Focal adhesion kinase and Wnt signaling regulate human ductal carcinoma in situ stem cell activity and response to radiotherapy. Stem Cells, 2015;33(2):327-41. 2015 [PMID: 25187396] (Bioassay, Human)
• Tsai H, Deng W, Lai W, Chiu W, Yang C, Tsai Y, Hwang S, Renshaw P Wnts enhance neurotrophin-induced neuronal differentiation in adult bone-marrow-derived mesenchymal stem cells via canonical and noncanonical signaling pathways. PLoS ONE, 2014;9(8):e104937. 2014 [PMID: 25170755] (Bioassay, Human)
• Hwang Y, Suk S, Shih Y, Seo T, Du B, Xie Y, Li Z, Varghese S WNT3A promotes myogenesis of human embryonic stem cells and enhances in vivo engraftment. Sci Rep, 2014;4(0):5916. 2014 [PMID: 25084050] (Bioassay, Human)
• Lee J, Heur M Interleukin-1beta-induced Wnt5a enhances human corneal endothelial cell migration through regulation of Cdc42 and RhoA. Mol Cell Biol, 2014;34(18):3535-45. 2014 [PMID: 25022753] (Bioassay, Human)
• Ao, Ying, Mich-Basso, Jocelyn, Lin, Bo, Yang, Lei High efficient differentiation of functional hepatocytes from porcine induced pluripotent stem cells. PLoS ONE, 2014;9(6):e100417. 2014 [PMID: 24949734] (Bioassay, Porcine)
• Cecchelli R, Aday S, Sevin E, Almeida C, Culot M, Dehouck L, Coisne C, Engelhardt B, Dehouck M, Ferreira L A stable and reproducible human blood-brain barrier model derived from hematopoietic stem cells. PLoS ONE, 2014;9(6):e99733. 2014 [PMID: 24936790] (Bioassay, Human)
• Skoczek D, Walczysko P, Horn N, Parris A, Clare S, Williams M, Sobolewski A Luminal microbes promote monocyte-stem cell interactions across a healthy colonic epithelium. J Immunol, 2014;193(1):439-51. 2014 [PMID: 24907348] (Bioassay, Human)
• Pethe, Prasad, Nagvenkar, Punam, Bhartiya, Deepa Polycomb group protein expression during differentiation of human embryonic stem cells into pancreatic lineage in vitro. BMC Cell Biol, 2014;15(0):18. 2014 [PMID: 24885493] (Bioassay, Human)
• Kilander M, Dahlstrom J, Schulte G Assessment of Frizzled 6 membrane mobility by FRAP supports G protein coupling and reveals WNT-Frizzled selectivity. Cell Signal, 2014;26(9):1943-9. 2014 [PMID: 24873871] (Bioassay, Human)
• Jin R, Liu W, Menezes S, Yue F, Zheng M, Kovacevic Z, Richardson D The metastasis suppressor NDRG1 modulates the phosphorylation and nuclear translocation of beta-catenin through mechanisms involving FRAT1 and PAK4. J Cell Sci, 2014;127(0):3116-30. 2014 [PMID: 24829151] (Bioassay, Human)
• Peng X, Yang L, Chang H, Dai G, Wang F, Duan X, Guo L, Zhang Y, Chen G Wnt/beta-catenin signaling regulates the proliferation and differentiation of mesenchymal progenitor cells through the p53 pathway. PLoS ONE, 2014;9(5):e97283. 2014 [PMID: 24819053] (Bioassay, Human)
• Hinton A, Hunter S, Afrikanova I, Jones G, Lopez A, Fogel G, Hayek A, King C sRNA-seq analysis of human embryonic stem cells and definitive endoderm reveals differentially expressed microRNAs and novel IsomiRs with distinct targets. Stem Cells, 2014;32(9):2360-72. 2014 [PMID: 24805944] (Bioassay, Human)
• Fuerer C, Nostro M, Constam D Nodal.Gdf1 heterodimers with bound prodomains enable serum-independent nodal signaling and endoderm differentiation. J Biol Chem, 2014;289(25):17854-71. 2014 [PMID: 24798330] (Bioassay, Human)
• Kang, Minyong, Kim, Hyeon Ho, Han, Yong-Mah Generation of bladder urothelium from human pluripotent stem cells under chemically defined serum- and feeder-free system. Int J Mol Sci, 2014;15(5):7139-57. 2014 [PMID: 24776760] (Bioassay, Human)
• Hillesheim A, Nordhoff C, Boergeling Y, Ludwig S, Wixler V beta-catenin promotes the type I IFN synthesis and the IFN-dependent signaling response but is suppressed by influenza A virus-induced RIG-I/NF-kappaB signaling. Cell Commun Signal, 2014;12(0):29. 2014 [PMID: 24767605] (Bioassay, Human)
• Ekstrom E, Bergenfelz C, von Bulow V, Serifler F, Carlemalm E, Jonsson G, Andersson T, Leandersson K WNT5A induces release of exosomes containing pro-angiogenic and immunosuppressive factors from malignant melanoma cells. Mol Cancer, 2014;13(0):88. 2014 [PMID: 24766647] (Bioassay, Human)
• Tsai T, Shih S, Ho T, Ma H, Liu M, Chen S, Tsao Y Pigment epithelium-derived factor 34-mer peptide prevents liver fibrosis and hepatic stellate cell activation through down-regulation of the PDGF receptor. PLoS ONE, 2014;9(4):e95443. 2014 [PMID: 24763086] (Bioassay, Rat)
• Emmrich S, Rasche M, Schoning J, Reimer C, Keihani S, Maroz A, Xie Y, Li Z, Schambach A, Reinhardt D, Klusmann J miR-99a/100~125b tricistrons regulate hematopoietic stem and progenitor cell homeostasis by shifting the balance between TGFbeta and Wnt signaling. Genes Dev, 2014;28(8):858-74. 2014 [PMID: 24736844] (Bioassay, Human)
• Kang, Minyong, Han, Yong-Mah Differentiation of human pluripotent stem cells into nephron progenitor cells in a serum and feeder free system. PLoS ONE, 2014;9(4):e94888. 2014 [PMID: 24728509] (Bioassay, Human)
• Nakamura I, Zakharia K, Banini B, Mikhail D, Kim T, Yang J, Moser C, Shaleh H, Thornburgh S, Walters I, Roberts L Brivanib attenuates hepatic fibrosis in vivo and stellate cell activation in vitro by inhibition of FGF, VEGF and PDGF signaling. PLoS ONE, 2014;9(4):e92273. 2014 [PMID: 24710173] (Bioassay, Human)
• Chen E, DeRan M, Ignatius M, Grandinetti K, Clagg R, McCarthy K, Lobbardi R, Brockmann J, Keller C, Wu X, Langenau D Glycogen synthase kinase 3 inhibitors induce the canonical WNT/beta-catenin pathway to suppress growth and self-renewal in embryonal rhabdomyosarcoma. Proc Natl Acad Sci U S A, 2014;111(14):5349-54. 2014 [PMID: 24706870] (Bioassay, Human)
• Cnossen W, te Morsche R, Hoischen A, Gilissen C, Chrispijn M, Venselaar H, Mehdi S, Bergmann C, Veltman J, Drenth J Whole-exome sequencing reveals LRP5 mutations and canonical Wnt signaling associated with hepatic cystogenesis. Proc Natl Acad Sci U S A, 2014;111(14):5343-8. 2014 [PMID: 24706814]
• Carmon K, Gong X, Yi J, Thomas A, Liu Q RSPO-LGR4 functions via IQGAP1 to potentiate Wnt signaling. Proc Natl Acad Sci U S A, 2014;111(13):E1221-9. 2014 [PMID: 24639526] (Bioassay, Human)
• Todaro M, Gaggianesi M, Catalano V, Benfante A, Iovino F, Biffoni M, Apuzzo T, Sperduti I, Volpe S, Cocorullo G, Gulotta G, Dieli F, De Maria R, Stassi G CD44v6 is a marker of constitutive and reprogrammed cancer stem cells driving colon cancer metastasis. Cell Stem Cell, 2014;14(3):342-56. 2014 [PMID: 24607406] (Bioassay, Human)
• Meinhardt G, Haider S, Haslinger P, Proestling K, Fiala C, Pollheimer J, Knofler M Wnt-dependent T-cell factor-4 controls human etravillous trophoblast motility. Endocrinology, 2014;155(5):1908-20. 2014 [PMID: 24605829] (Bioassay, Human)
• Guezguez A, Pare F, Benoit Y, Basora N, Beaulieu J Modulation of stemness in a human normal intestinal epithelial crypt cell line by activation of the WNT signaling pathway. Exp Cell Res, 2014;322(2):355-64. 2014 [PMID: 24534551] (Bioassay, Human)
• Lin, I-Ying, Chiu, Feng-Lan, Yeang, Chen-Hsi, Chen, Hsin-Fu, Chuang, Ching-Yu, Yang, Shii-Yi, Hou, Pei-Shan, Sintupisut, Nardnisa, Ho, Hong-Ner, Kuo, Hung-Chi, Lin, Kuo-I Suppression of the SOX2 neural effector gene by PRDM1 promotes human germ cell fate in embryonic stem cells. Stem Cell Reports, 2014;2(2):189-204. 2014 [PMID: 24527393] (Bioassay, Human)
• Wu C, Qiu S, Lu L, Zou J, Li W, Wang O, Zhao H, Wang H, Tang J, Chen L, Xu T, Sun Z, Liao W, Luo G, Lu X RSPO2-LGR5 signaling has tumour-suppressive activity in colorectal cancer. Nat Commun, 2014;5(0):3149. 2014 [PMID: 24476626]
• van den Bosch M, Blom A, van Lent P, van Beuningen H, Blaney Davidson E, van der Kraan P, van den Berg W Canonical Wnt signaling skews TGF-beta signaling in chondrocytes towards signaling via ALK1 and Smad 1/5/8. Cell Signal, 2014;26(5):951-8. 2014 [PMID: 24463008] (Bioassay, Human)
• Aloia L, Di Stefano B, Sessa A, Morey L, Santanach A, Gutierrez A, Cozzuto L, Benitah S, Graf T, Broccoli V, Di Croce L Zrf1 is required to establish and maintain neural progenitor identity. Genes Dev, 2014;28(2):182-97. 2014 [PMID: 24449271] (Bioassay, Human)
• Massumi M, Hoveizi E, Baktash P, Hooti A, Ghazizadeh L, Nadri S, Pourasgari F, Hajarizadeh A, Soleimani M, Nabiuni M, Khorramizadeh M Efficient programming of human eye conjunctiva-derived induced pluripotent stem (ECiPS) cells into definitive endoderm-like cells. Exp Cell Res, 2014;322(1):51-61. 2014 [PMID: 24434355] (Bioassay, Human)
• Dhamdhere, Girija R, Fang, Mark Y, Jiang, Jie, Lee, Katherin, Cheng, Du, Olveda, Rebecca, Liu, Bo, Mulligan, Kimberle, Carlson, Jeffery, Ransom, Ryan C, Weis, William, Helms, Jill A Drugging a stem cell compartment using Wnt3a protein as a therapeutic. PLoS ONE, 2014;9(1):e83650. 2014 [PMID: 24400074] (Bioassay, Human)
• Cechin S, Alvarez-Cubela S, Giraldo J, Molano R, Villate S, Ricordi C, Pileggi A, Inverardi L, Fraker C, Dominguez-Bendala J Influence of in vitro and in vivo oxygen modulation on beta cell differentiation from human embryonic stem cells. Stem Cells Transl Med, 2014;3(3):277-89. 2014 [PMID: 24375542] (Bioassay, Human)
• Lam A, Freedman B, Morizane R, Lerou P, Valerius M, Bonventre J Rapid and efficient differentiation of human pluripotent stem cells into intermediate mesoderm that forms tubules expressing kidney proximal tubular markers. J Am Soc Nephrol, 2014;25(6):1211-25. 2014 [PMID: 24357672] (Bioassay, Human)
• Hannan, Nicholas, Fordham, Robert P, Syed, Yasir A, Moignard, Victoria, Berry, Andrew, Bautista, Ruben, Hanley, Neil A, Jensen, Kim B, Vallier, Ludovic Generation of multipotent foregut stem cells from human pluripotent stem cells. Stem Cell Reports, 2013;1(4):293-306. 2013 [PMID: 24319665] (Bioassay, Human)
• Listerman I, Gazzaniga F, Blackburn E An investigation of the effects of the core protein telomerase reverse transcriptase on Wnt signaling in breast cancer cells. Mol Cell Biol, 2014;34(2):280-9. 2014 [PMID: 24216762] (Bioassay, Human)
• Goto T, Sato A, Adachi S, Iemura S, Natsume T, Shibuya H IQGAP1 protein regulates nuclear localization of beta-catenin via importin-beta5 protein in Wnt signaling. J Biol Chem, 2013;288(51):36351-60. 2013 [PMID: 24196961] (cell culture, Human)
• Jiang Y, Dai J, Zhang H, Sottnik J, Keller J, Escott K, Sanganee H, Yao Z, McCauley L, Keller E Activation of the Wnt pathway through AR79, a GSK3beta inhibitor, promotes prostate cancer growth in soft tissue and bone. Mol Cancer Res, 2013;11(12):1597-610. 2013 [PMID: 24088787]
• Lin P, Hung S, Yang Y, Liao L, Hsieh Y, Yen H, Lu H, Lee M, Chu I, Hwang S A synthetic peptide-acrylate surface for production of insulin-producing cells from human embryonic stem cells. Stem Cells Dev, 2014;23(4):372-9. 2014 [PMID: 24083371] (Bioassay, Human)
• Rezania A, Bruin J, Xu J, Narayan K, Fox J, O'Neil J, Kieffer T Enrichment of human embryonic stem cell-derived NKX6.1-expressing pancreatic progenitor cells accelerates the maturation of insulin-secreting cells in vivo. Stem Cells, 2013;31(11):2432-42. 2013 [PMID: 23897760] (Bioassay, Human)
• Rasmussen N, Wright T, Brooks S, Hacker K, Debebe Z, Sendor A, Walker M, Major M, Green J, Wahl G, Rathmell W Receptor tyrosine kinase-like orphan receptor 2 (Ror2) expression creates a poised state of Wnt signaling in renal cancer. J Biol Chem, 2013;288(36):26301-10. 2013 [PMID: 23893409] (Bioassay, Human)
• Loh Y, Hedditch E, Baker L, Jary E, Ward R, Ford C The Wnt signalling pathway is upregulated in an in vitro model of acquired tamoxifen resistant breast cancer. BMC Cancer, 2013;13(0):174. 2013 [PMID: 23547709] (Bioassay, Human)
• Hernandez-Maqueda J, Luna-Ulloa L, Santoyo-Ramos P, Castaneda-Patlan M, Robles-Flores M Protein kinase C delta negatively modulates canonical Wnt pathway and cell proliferation in colon tumor cell lines. PLoS ONE, 2013;8(3):e58540. 2013 [PMID: 23520519] (Bioassay, Human)
• Greer Y, Fields A, Brown A, Rubin J Atypical protein kinase Ciota is required for Wnt3a-dependent neurite outgrowth and binds to phosphorylated dishevelled 2. J Biol Chem, 2013;288(13):9438-46. 2013 [PMID: 23396968] (Bioassay, Human)
• Tahir S, Yang G, Goltsov A, Song K, Ren C, Wang J, Chang W, Thompson T Caveolin-1-LRP6 signaling module stimulates aerobic glycolysis in prostate cancer. Cancer Res, 2013;73(6):1900-11. 2013 [PMID: 23302227] (Bioassay, Human)
• Thatava T, Kudva Y, Edukulla R, Squillace K, De Lamo J, Khan Y, Sakuma T, Ohmine S, Terzic A, Ikeda Y Intrapatient variations in type 1 diabetes-specific iPS cell differentiation into insulin-producing cells. Mol Ther, 2013;21(1):228-39. 2013 [PMID: 23183535] (Bioassay, Human)
• Cripto-1 enhances the canonical Wnt/β-catenin signaling pathway by binding to LRP5 and LRP6 co-receptors. Cell Signal, 2013;25(1):178-89. 2013 [PMID: 23022962] (Bioassay, Human)
• Sui L, Mfopou J, Geens M, Sermon K, Bouwens L FGF signaling via MAPK is required early and improves Activin A-induced definitive endoderm formation from human embryonic stem cells. Biochem Biophys Res Commun, 2012;426(3):380-5. 2012 [PMID: 22960178] (Bioassay, Human)
• Aad, Pauline, Echternkamp, Sherrill, Sypherd, David D, Schreiber, Nicole B, Spicer, Leon J The hedgehog system in ovarian follicles of cattle selected for twin ovulations and births: evidence of a link between the IGF and hedgehog systems. Biol Reprod, 2012;87(4):79. 2012 [PMID: 22811575] (Bioassay, Bovine)
• Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012;487(7408):505-9. 2012 [PMID: 22763448] (Bioassay, Human)
• Cohen E, Miller M, Wang Z, Moon R, Morrisey E Wnt5a and Wnt11 are essential for second heart field progenitor development. Development, 2012;139(11):1931-40. 2012 [PMID: 22569553] (Bioassay, Mouse)
• Erceg S, Lukovic D, Moreno-Manzano V, Stojkovic M, Bhattacharya S Derivation of cerebellar neurons from human pluripotent stem cells. Curr Protoc Stem Cell Biol, 2012;20(0):1H.51. 2012 [PMID: 22415839] (Bioassay, Human)
• Prabakar K, Dominguez-Bendala J, Molano R, Pileggi A, Villate S, Ricordi C, Inverardi L Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells. Cell Transplant, 2012;21(6):1321-39. 2012 [PMID: 22195604] (cell culture, Human)
• Ichii, Michiko, Frank, Mark Bar, Iozzo, Renato V, Kincade, Paul W The canonical Wnt pathway shapes niches supportive of hematopoietic stem/progenitor cells. Blood, 2012;119(7):1683-92. 2012 [PMID: 22117039] (Bioassay, Human)
• Liu N, Shi S, Deng M, Tang L, Zhang G, Liu N, Ding B, Liu W, Liu Y, Shi H, Liu L, Jin Y High levels of beta-catenin signaling reduce osteogenic differentiation of stem cells in inflammatory microenvironments through inhibition of the noncanonical Wnt pathway. J. Bone Miner. Res., 2011;26(9):2082-95. 2011 [PMID: 21638320] (Bioassay, Human)
• Spence JR, Mayhew CN, Rankin SA Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro. Nature, 2011;470(7332):105-9. 2011 [PMID: 21151107] (Bioassay, Human)
• Kohler EE, Cowan CE, Chatterjee I NANOG induction of fetal liver kinase-1 (FLK1) transcription regulates endothelial cell proliferation and angiogenesis. Blood, 2011;117(5):1761-9. 2011 [PMID: 21119109] (Bioassay, Human)
• Gudjonsson JE, Johnston A, Stoll SW Evidence for altered Wnt signaling in psoriatic skin. J. Invest. Dermatol., 2010;130(7):1849-59. 2010 [PMID: 20376066] (Bioassay, Human)
• Liu G, Vijayakumar S, Grumolato L, Arroyave R, Qiao H, Akiri G, Aaronson SA Canonical Wnts function as potent regulators of osteogenesis by human mesenchymal stem cells. J. Cell Biol., 2009;185(1):67-75. 2009 [PMID: 19349579] (Bioassay, Human)

Bioinformatics

• Gene Symbol: WNT3A
• Uniprot:
  • NP_149122(Human)