Recombinant Human Wnt-3a Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human Wnt-3a Protein Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 5-25 ng/mL. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is <500 ng/mL. Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line.
Optimal concentrations should be determined by each laboratory for each application.
Source
Chinese Hamster Ovary cell line, CHO-derived human Wnt-3a protein
Ser19-Lys352
Accession #
N-terminal Sequence
Ser19
Protein/Peptide Type
Recombinant Proteins
Gene
WNT3A
Purity
>75%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
37.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
40 kDa, reducing conditions
Publications
Read Publications using
5036-WN in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
Purity
>75%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Wnt-3a Protein

  • MGC119418
  • MGC119419
  • MGC119420
  • protein Wnt-3a
  • wingless-type MMTV integration site family, member 3A
  • Wnt3a
  • Wnt-3a

Background

Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes (1). Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta -catenin levels (2). As intracellular beta -catenin levels rise, beta -catenin binds to TCF/LEF transcription factors leading to expression of Wnt target genes (3). Endo-IWR 1 (Catalog # 3532, # PSM1324) is a cell-permeant small molecule inhibitor of Axin turnover that suppresses Wnt signal transduction by stabilizing the beta -catenin destruction complex (4). Wnt-3a is a 44 kDa secreted hydrophobic glycoprotein containing a conserved pattern of 24 cysteine residues (5). Wnt-3a has two N-linked glycosylation sites (Asn 87, Asn 298), and Ser 209 is modified with palmitoleic acid (6). Glycosylation and acylation are essential for efficient Wnt secretion and biological activity, respectively (6, 7). Human Wnt-3a shares 96% amino acid (aa) identity with mouse mouse, bovine and canine Wnt-3a, and 89%, 86% and 84% aa identity with chicken, Xenopus and zebrafish Wnt-3a, respectively. It also shares 87% aa identity with Wnt3. During embryonic development, Wnt-3a is necessary for proper development of the hippocampus, anterior-posterior patterning, somite development, and tailbud formation (9-12). Wnt-3a also promotes self-renewal of hematopoietic stem cells, neural stem cells, and embryonic stem cells (13, 14).

  1. Willert, K. and Nusse, R. (2012) Cold Spring Harb. Perspect. Biol. 4:a007864.
  2. MacDonald, B.T. and X. He (2012) Cold Spring Harb. Perspect. Biol. 4:a007880.
  3. Korinek, V. et al. (1997) Science 275:1784.
  4. Chen, B. et al. (2009) Nat. Chem. Biol. 5:100.
  5. Smolich, B.D. et al. (1993) Mol. Biol. Cell 4:1267.
  6. Takada, R. et al. (2006) Dev. Cell 11:791.
  7. Komekado, H. (2007) Genes Cells 12:521.
  8. Dunty Jr. W. C. et al. (2008) Development 135:85.
  9. Ikeya, M. and S. Takada (2001) Mech. Dev. 103:27.
  10. Lee, S. M. et al. (2000) Development 127:457.
  11. Takada, S. et al. (1994) Genes Dev. 8:174.
  12. Willert, K. et al. (2003) Nature 423:6938.
  13. Kalani, M.Y. et al. (2008) Proc. Natl. Acad. Sci. USA 105:16970.
  14. Ten Berge, D. et al. (2011) Nat. Cell Biol. 13:1070.

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Publications for Wnt-3a (5036-WN)(151)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, Porcine.

We have publications tested in 2 applications: Bioassay, cell culture.


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Bioassay
(144)
cell culture
(3)
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Human
(134)
Mouse
(7)
Rat
(2)
Bovine
(2)
Porcine
(2)
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Showing Publications 1 - 10 of 151. Show All 151 Publications.
Publications using 5036-WN Applications Species
BN Nakamura, A Glazier, MG Kattah, B Duong, Y Jia, D Campo, L Shao A20 regulates canonical wnt-signaling through an interaction with RIPK4 PLoS ONE, 2018;13(5):e0195893. 2018 [PMID: 29718933] (Bioassay, Human) Bioassay Human
M Amin, Y Kushida, S Wakao, M Kitada, K Tatsumi, M Dezawa Cardiotrophic Growth Factor-Driven Induction of Human Muse Cells Into Cardiomyocyte-Like Phenotype Cell Transplant, 2018;27(2):285-298. 2018 [PMID: 29637816] (Bioassay, Human) Bioassay Human
H He, F Du, Y He, Z Wei, C Meng, Y Xu, H Zhou, N Wang, XG Luo, W Ma, TC Zhang The Wnt-?-catenin signaling regulated MRTF-A transcription to activate migration-related genes in human breast cancer cells Oncotarget, 2018;9(20):15239-15251. 2018 [PMID: 29632640] (Bioassay, Human) Bioassay Human
XG Li, Z Wang, RQ Chen, HL Fu, CQ Gao, HC Yan, GX Xing, XQ Wang LGR5 and BMI1 Increase Pig Intestinal Epithelial Cell Proliferation by Stimulating WNT/?-Catenin Signaling Int J Mol Sci, 2018;19(4):. 2018 [PMID: 29601474] (Bioassay, Porcine) Bioassay Porcine
HJ Lee, J Li, RE Vickman, J Li, R Liu, A Durkes, BD Elzey, S Yue, X Liu, TL Ratliff, JX Cheng Cholesterol Esterification Inhibition Suppresses Prostate Cancer Metastasis by Impairing the Wnt/?-catenin Pathway Mol. Cancer Res., 2018;0(0):. 2018 [PMID: 29545473] (Bioassay, Human) Bioassay Human
S Condello, LE Sima, C Ivan, H Cardenas, GE Schiltz, RK Mishra, D Matei Tissue transglutaminase regulates interactions between ovarian cancer stem cells and the tumor niche Cancer Res., 2018;0(0):. 2018 [PMID: 29510995] (Bioassay, Human) Bioassay Human
M Szemes, A Greenhough, Z Melegh, S Malik, A Yuksel, D Catchpoole, K Gallacher, M Kollareddy, JH Park, K Malik Wnt Signalling Drives Context-Dependent Differentiation or Proliferation in Neuroblastoma Neoplasia, 2018;20(4):335-350. 2018 [PMID: 29505958] (Bioassay, Human) Bioassay Human
S Kokabu, V Rosen BMP3 expression by osteoblast lineage cells is regulated by canonical Wnt signaling FEBS Open Bio, 2018;8(2):168-176. 2018 [PMID: 29435407] (Bioassay, Mouse) Bioassay Mouse
X Wang, M Sterr, I Burtscher, S Chen, A Hieronimus, F Machicao, H Staiger, HU Häring, G Lederer, T Meitinger, FM Cernilogar, G Schotta, M Irmler, J Beckers, M Hrab? de A, M Ray, CVE Wright, M Bakhti, H Lickert Genome-wide analysis of PDX1 target genes in human pancreatic progenitors Mol Metab, 2018;0(0):. 2018 [PMID: 29396371] (Bioassay, Human) Bioassay Human
Y Sui, Z Liu, SH Park, SE Thatcher, B Zhu, JP Fernandez, H Molina, PA Kern, C Zhou IKK? is a ?-catenin kinase that regulates mesenchymal stem cell differentiation JCI Insight, 2018;3(2):. 2018 [PMID: 29367460] (Bioassay, Mouse) Bioassay Mouse
Show All 151 Publications.

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Bioinformatics

Gene Symbol WNT3A
Entrez
Uniprot