Recombinant Human Wnt-3a Protein

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Recombinant Human Wnt-3a (Catalog # 5036-WN) induces alkaline phosphatase production by the MC3T3-E1 mouse preosteoblast cell line. The ED50 is 1.7-fold better with more than twice the maximum response compared to the ...read more

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human Wnt-3a Protein Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 5-25 ng/mL. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is <500 ng/mL. Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line.
Optimal concentrations should be determined by each laboratory for each application.
Source
Chinese Hamster Ovary cell line, CHO-derived human Wnt-3a protein
Ser19-Lys352
Accession #
N-terminal Sequence
Ser19
Protein/Peptide Type
Recombinant Proteins
Gene
WNT3A
Purity
>75%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
37.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
40 kDa, reducing conditions
Publications
Read Publications using
5036-WN in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
Purity
>75%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Wnt-3a Protein

  • MGC119418
  • MGC119419
  • MGC119420
  • protein Wnt-3a
  • wingless-type MMTV integration site family, member 3A
  • Wnt3a
  • Wnt-3a

Background

Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes (1). Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta -catenin levels (2). As intracellular beta -catenin levels rise, beta -catenin binds to TCF/LEF transcription factors leading to expression of Wnt target genes (3). Endo-IWR 1 (Catalog # 3532, # PSM1324) is a cell-permeant small molecule inhibitor of Axin turnover that suppresses Wnt signal transduction by stabilizing the beta -catenin destruction complex (4). Wnt-3a is a 44 kDa secreted hydrophobic glycoprotein containing a conserved pattern of 24 cysteine residues (5). Wnt-3a has two N-linked glycosylation sites (Asn 87, Asn 298), and Ser 209 is modified with palmitoleic acid (6). Glycosylation and acylation are essential for efficient Wnt secretion and biological activity, respectively (6, 7). Human Wnt-3a shares 96% amino acid (aa) identity with mouse mouse, bovine and canine Wnt-3a, and 89%, 86% and 84% aa identity with chicken, Xenopus and zebrafish Wnt-3a, respectively. It also shares 87% aa identity with Wnt3. During embryonic development, Wnt-3a is necessary for proper development of the hippocampus, anterior-posterior patterning, somite development, and tailbud formation (9-12). Wnt-3a also promotes self-renewal of hematopoietic stem cells, neural stem cells, and embryonic stem cells (13, 14).

  1. Willert, K. and Nusse, R. (2012) Cold Spring Harb. Perspect. Biol. 4:a007864.
  2. MacDonald, B.T. and X. He (2012) Cold Spring Harb. Perspect. Biol. 4:a007880.
  3. Korinek, V. et al. (1997) Science 275:1784.
  4. Chen, B. et al. (2009) Nat. Chem. Biol. 5:100.
  5. Smolich, B.D. et al. (1993) Mol. Biol. Cell 4:1267.
  6. Takada, R. et al. (2006) Dev. Cell 11:791.
  7. Komekado, H. (2007) Genes Cells 12:521.
  8. Dunty Jr. W. C. et al. (2008) Development 135:85.
  9. Ikeya, M. and S. Takada (2001) Mech. Dev. 103:27.
  10. Lee, S. M. et al. (2000) Development 127:457.
  11. Takada, S. et al. (1994) Genes Dev. 8:174.
  12. Willert, K. et al. (2003) Nature 423:6938.
  13. Kalani, M.Y. et al. (2008) Proc. Natl. Acad. Sci. USA 105:16970.
  14. Ten Berge, D. et al. (2011) Nat. Cell Biol. 13:1070.

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Publications for Wnt-3a (5036-WN)(220)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Bovine, Fish - Oryzias latipes (Medaka/Rice Fish), Porcine.

We have publications tested in 6 applications: Bioassay, Cell Culture, Immunoassay Development, In Vivo, Stimulation, Tissue Culture.


Filter By Application
Bioassay
(194)
Cell Culture
(16)
Immunoassay Development
(1)
In Vivo
(1)
Stimulation
(1)
Tissue Culture
(1)
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Filter By Species
Human
(194)
Mouse
(12)
Rat
(4)
Bovine
(2)
Fish - Oryzias latipes (Medaka/Rice Fish)
(1)
Porcine
(3)
All Species
Showing Publications 1 - 10 of 220. Show All 220 Publications.
Publications using 5036-WN Applications Species
Y Wu, M Sarkissyan, O Ogah, J Kim, JV Vadgama Expression of MALAT1 Promotes Trastuzumab Resistance in HER2 Overexpressing Breast Cancers Cancers (Basel), 2020;12(7):. 2020 [PMID: 32708561] (Cell Culture, Human) Cell Culture Human
X Shi, W Lin, X Gao, W Xie, JA Golden, T Tao Identification and validation of the phosphorylation sites on Aristaless-related homeobox protein Biosci. Rep., 2020;40(7):. 2020 [PMID: 32608477] (Bioassay, Human) Bioassay Human
W Kawarazaki, R Mizuno, M Nishimoto, N Ayuzawa, D Hirohama, K Ueda, F Kawakami-M, S Oba, T Marumo, T Fujita Salt causes aging-associated hypertension via vascular Wnt5a under Klotho deficiency J. Clin. Invest., 2020;0(0):. 2020 [PMID: 32597829] (Cell Culture, Human) Cell Culture Human
FH Brasil da, MS Lewis, A Truong, DD Carson, MC Farach-Car SULF1 suppresses Wnt3A-driven growth of bone metastatic prostate cancer in perlecan-modified 3D cancer-stroma-macrophage triculture models PLoS ONE, 2020;15(5):e0230354. 2020 [PMID: 32413029] (Cell Culture, Human) Cell Culture Human
M Bowers, T Liang, D Gonzalez-B, S Zocher, BN Jaeger, WJ Kovacs, C Röhrl, KML Cramb, J Winterer, M Kruse, S Dimitrieva, RW Overall, T Wegleiter, H Najmabadi, CF Semenkovic, G Kempermann, C Földy, S Jessberger FASN-Dependent Lipid Metabolism Links Neurogenic Stem/Progenitor Cell Activity to Learning and Memory Deficits Cell Stem Cell, 2020;0(0):. 2020 [PMID: 32386572] (Bioassay, Human) Bioassay Human
A Fleischer, S Vallejo-Dí, JM Martín-Fer, A Sánchez-Gi, M Castresana, A Del Pozo, A Esquisabel, S Ávila, JL Castrillo, E Gaínza, JL Pedraz, M Viñas, D Bachiller iPSC-Derived Intestinal Organoids from Cystic Fibrosis Patients Acquire CFTR Activity upon TALEN-Mediated Repair of the p.F508del Mutation Mol Ther Methods Clin Dev, 2020;17(0):858-870. 2020 [PMID: 32373648] (Cell Culture, Human) Cell Culture Human
F He, N Li, HB Huang, JB Wang, XF Yang, HD Wang, W Huang, FR Li LSD1 inhibition yields functional insulin-producing cells from human embryonic stem cells Stem Cell Res Ther, 2020;11(1):163. 2020 [PMID: 32345350] (Bioassay, Human) Bioassay Human
E Flores-Her, DM Velázquez, MC Castañeda-, G Fuentes-Ga, G Fonseca-Ca, JK Yamamoto-F, MT Romero-Avi, JA García-Sái, M Robles-Flo Canonical and non-canonical Wnt signaling are simultaneously activated by Wnts in colon cancer cells Cell. Signal., 2020;72(0):109636. 2020 [PMID: 32283254] (Bioassay, Human) Bioassay Human
Q Li, Y Sun, GK Jarugumill, S Liu, K Dang, JL Cotton, J Xiol, PY Chan, M DeRan, L Ma, R Li, LJ Zhu, JH Li, AB Leiter, YT Ip, FD Camargo, X Luo, RL Johnson, X Wu, J Mao Lats1/2 Sustain Intestinal Stem Cells and Wnt Activation through TEAD-Dependent and Independent Transcription Cell Stem Cell, 2020;26(5):675-692.e8. 2020 [PMID: 32259481] (Cell Culture, Human) Cell Culture Human
S Yoshida, H Miwa, T Kawachi, S Kume, K Takahashi Generation of intestinal organoids derived from human pluripotent stem cells for drug testing Sci Rep, 2020;10(1):5989. 2020 [PMID: 32249832] (Bioassay, Human) Bioassay Human
Show All 220 Publications.

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Bioinformatics

Gene Symbol WNT3A
Uniprot