Recombinant Human Wnt-3a Protein


Recombinant Human Wnt-3a (Catalog # 5036-WN) induces alkaline phosphatase production by the MC3T3-E1 mouse preosteoblast cell line. The ED50 is 1.7-fold better with more than twice the maximum response compared to the more

Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human Wnt-3a Protein Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 5-25 ng/mL. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is <500 ng/mL. Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line.
Optimal concentrations should be determined by each laboratory for each application.
Chinese Hamster Ovary cell line, CHO-derived human Wnt-3a protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>75%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


Theoretical MW
37.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
40 kDa, reducing conditions
Read Publications using
5036-WN in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
>75%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Wnt-3a Protein

  • MGC119418
  • MGC119419
  • MGC119420
  • protein Wnt-3a
  • wingless-type MMTV integration site family, member 3A
  • Wnt3a
  • Wnt-3a


Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes (1). Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta -catenin levels (2). As intracellular beta -catenin levels rise, beta -catenin binds to TCF/LEF transcription factors leading to expression of Wnt target genes (3). Endo-IWR 1 (Catalog # 3532, # PSM1324) is a cell-permeant small molecule inhibitor of Axin turnover that suppresses Wnt signal transduction by stabilizing the beta -catenin destruction complex (4). Wnt-3a is a 44 kDa secreted hydrophobic glycoprotein containing a conserved pattern of 24 cysteine residues (5). Wnt-3a has two N-linked glycosylation sites (Asn 87, Asn 298), and Ser 209 is modified with palmitoleic acid (6). Glycosylation and acylation are essential for efficient Wnt secretion and biological activity, respectively (6, 7). Human Wnt-3a shares 96% amino acid (aa) identity with mouse mouse, bovine and canine Wnt-3a, and 89%, 86% and 84% aa identity with chicken, Xenopus and zebrafish Wnt-3a, respectively. It also shares 87% aa identity with Wnt3. During embryonic development, Wnt-3a is necessary for proper development of the hippocampus, anterior-posterior patterning, somite development, and tailbud formation (9-12). Wnt-3a also promotes self-renewal of hematopoietic stem cells, neural stem cells, and embryonic stem cells (13, 14).

  1. Willert, K. and Nusse, R. (2012) Cold Spring Harb. Perspect. Biol. 4:a007864.
  2. MacDonald, B.T. and X. He (2012) Cold Spring Harb. Perspect. Biol. 4:a007880.
  3. Korinek, V. et al. (1997) Science 275:1784.
  4. Chen, B. et al. (2009) Nat. Chem. Biol. 5:100.
  5. Smolich, B.D. et al. (1993) Mol. Biol. Cell 4:1267.
  6. Takada, R. et al. (2006) Dev. Cell 11:791.
  7. Komekado, H. (2007) Genes Cells 12:521.
  8. Dunty Jr. W. C. et al. (2008) Development 135:85.
  9. Ikeya, M. and S. Takada (2001) Mech. Dev. 103:27.
  10. Lee, S. M. et al. (2000) Development 127:457.
  11. Takada, S. et al. (1994) Genes Dev. 8:174.
  12. Willert, K. et al. (2003) Nature 423:6938.
  13. Kalani, M.Y. et al. (2008) Proc. Natl. Acad. Sci. USA 105:16970.
  14. Ten Berge, D. et al. (2011) Nat. Cell Biol. 13:1070.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, IHC
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ChIP, CyTOF-ready, ICC, ICFlow
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, PLA, RNAi, S-ELISA
Species: Hu
Applications: WB, Flow, CyTOF-ready
Species: Hu
Applications: WB, ELISA, Flow, CyTOF-ready
Species: Hu, Mu, Rt
Applications: WB
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ChIP, CyTOF-ready, ICC, ICFlow
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-P

Publications for Wnt-3a (5036-WN)(210)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Bovine, Fish - Oryzias latipes (Medaka/Rice Fish), Porcine.

We have publications tested in 6 applications: Bioassay, Cell Culture, Immunoassay Development, In Vivo, Stimulation, Tissue Culture.

Filter By Application
Cell Culture
Immunoassay Development
In Vivo
Tissue Culture
All Applications
Filter By Species
Fish - Oryzias latipes (Medaka/Rice Fish)
All Species
Showing Publications 1 - 10 of 210. Show All 210 Publications.
Publications using 5036-WN Applications Species
P Kozielewic, A Turku, CF Bowin, J Petersen, J Valnohova, MCA Cañizal, Y Ono, A Inoue, C Hoffmann, G Schulte Structural insight into small molecule action on Frizzleds Nat Commun, 2020;11(1):414. 2020 [PMID: 31964872] (Cell Culture, Human) Cell Culture Human
KH Grinnemo, M Löfling, L Nathanson, R Baumgartne, DFJ Ketelhuth, V Beljanski, LC Davies, C Österholm Immunomodulatory effects of interferon-gamma on human fetal cardiac mesenchymal stromal cells Stem Cell Res Ther, 2019;10(1):371. 2019 [PMID: 31801632] (Bioassay, Human) Bioassay Human
AP Singh, YH Hung, MT Shanahan, M Kanke, A Bonfini, MK Dame, M Biraud, BCE Peck, OO Oyesola, JM Freund, RL Cubitt, EG Curry, LM Gonzalez, GA Bewick, ED Tait-Wojno, NA Kurpios, S Ding, JR Spence, CM Dekaney, N Buchon, P Sethupathy Enteroendocrine progenitor cell enriched miR-7 regulates intestinal epithelial proliferation in an Xiap-dependent manner Cell Mol Gastroenterol Hepatol, 2019;0(0):. 2019 [PMID: 31756561] (Tissue Culture, Porcine) Tissue Culture Porcine
Z Ye, S Mittag, M Schmidt, A Simm, R Horstkorte, O Huber Wnt Glycation Inhibits Canonical Signaling Cells, 2019;8(11):. 2019 [PMID: 31731544] (Cell Culture, Human) Cell Culture Human
R Eyre, DG Alférez, A Santiago-G, K Spence, JC McConnell, C Hart, BM Simões, D Lefley, C Tulotta, J Storer, A Gurney, N Clarke, M Brown, SJ Howell, AH Sims, G Farnie, PD Ottewell, RB Clarke Microenvironmental IL1beta promotes breast cancer metastatic colonisation in the bone via activation of Wnt signalling Nat Commun, 2019;10(1):5016. 2019 [PMID: 31676788] (Cell Culture, Human) Cell Culture Human
RR Stine, AP Sakers, T TeSlaa, M Kissig, ZE Stine, CW Kwon, L Cheng, HW Lim, KH Kaestner, JD Rabinowitz, P Seale PRDM16 Maintains Homeostasis of the Intestinal Epithelium by Controlling Region-Specific Metabolism Cell Stem Cell, 2019;0(0):. 2019 [PMID: 31564549] (Bioassay, Mouse) Bioassay Mouse
V Lungova, X Chen, Z Wang, C Kendziorsk, SL Thibeault Human induced pluripotent stem cell-derived vocal fold mucosa mimics development and responses to smoke exposure Nat Commun, 2019;10(1):4161. 2019 [PMID: 31551422] (Bioassay, Human) Bioassay Human
F Tonon, GG Giobbe, A Zambon, C Luni, O Gagliano, A Floreani, G Grassi, N Elvassore In vitro metabolic zonation through oxygen gradient on a chip Sci Rep, 2019;9(1):13557. 2019 [PMID: 31537830] (Bioassay, Human) Bioassay Human
CL Hammond, E Roztocil, RP Phipps, SE Feldon, CF Woeller Proton pump inhibitors attenuate myofibroblast formation associated with thyroid eye disease through the aryl hydrocarbon receptor PLoS ONE, 2019;14(9):e0222779. 2019 [PMID: 31536596] (Bioassay, Human) Bioassay Human
S Akbari, GG Sevinç, N Ersoy, O Basak, K Kaplan, K Sevinç, E Ozel, B Sengun, E Enustun, B Ozcimen, A Bagriyanik, N Arslan, TT Önder, E Erdal Robust, Long-Term Culture of Endoderm-Derived Hepatic Organoids for Disease Modeling Stem Cell Reports, 2019;13(4):627-641. 2019 [PMID: 31522975] (Cell Culture, Human) Cell Culture Human
Show All 210 Publications.

Reviews for Wnt-3a (5036-WN) (0)

There are no reviews for Wnt-3a (5036-WN). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Wnt-3a (5036-WN) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional Wnt-3a Products

Bioinformatics Tool for Wnt-3a (5036-WN)

Discover related pathways, diseases and genes to Wnt-3a (5036-WN). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Wnt-3a (5036-WN)

Discover more about diseases related to Wnt-3a (5036-WN).

Pathways for Wnt-3a (5036-WN)

View related products by pathway.

PTMs for Wnt-3a (5036-WN)

Learn more about PTMs related to Wnt-3a (5036-WN).

Blogs on Wnt-3a

There are no specific blogs for Wnt-3a, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Human Wnt-3a Protein and receive a gift card or discount.


Gene Symbol WNT3A