Recombinant Human Wnt-3a Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications BA

Order Details

Recombinant Human Wnt-3a Protein Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 5-25 ng/mL. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is <500 ng/mL. Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line.
Optimal concentrations should be determined by each laboratory for each application.
Source
Chinese Hamster Ovary cell line, CHO-derived
Ser19-Lys352
Accession #
N-terminal Sequence
Ser19
Protein/Peptide Type
Recombinant Proteins
Gene
WNT3A
Purity
>75%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
37.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
40 kDa, reducing conditions
Publications
Read Publications using
5036-WN in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
Purity
>75%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Wnt-3a Protein

  • MGC119418
  • MGC119419
  • MGC119420
  • protein Wnt-3a
  • wingless-type MMTV integration site family, member 3A
  • Wnt3a
  • Wnt-3a

Background

Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes (1). Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta -catenin levels (2). As intracellular beta -catenin levels rise, beta -catenin binds to TCF/LEF transcription factors leading to expression of Wnt target genes (3). Endo-IWR 1 (Catalog # 3532, # PSM1324) is a cell-permeant small molecule inhibitor of Axin turnover that suppresses Wnt signal transduction by stabilizing the beta -catenin destruction complex (4). Wnt-3a is a 44 kDa secreted hydrophobic glycoprotein containing a conserved pattern of 24 cysteine residues (5). Wnt-3a has two N-linked glycosylation sites (Asn 87, Asn 298), and Ser 209 is modified with palmitoleic acid (6). Glycosylation and acylation are essential for efficient Wnt secretion and biological activity, respectively (6, 7). Human Wnt-3a shares 96% amino acid (aa) identity with mouse mouse, bovine and canine Wnt-3a, and 89%, 86% and 84% aa identity with chicken, Xenopus and zebrafish Wnt-3a, respectively. It also shares 87% aa identity with Wnt3. During embryonic development, Wnt-3a is necessary for proper development of the hippocampus, anterior-posterior patterning, somite development, and tailbud formation (9-12). Wnt-3a also promotes self-renewal of hematopoietic stem cells, neural stem cells, and embryonic stem cells (13, 14).

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Publications for (117)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, Porcine.

We have publications tested in 2 applications: Bioassay, cell culture.


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Bioassay
(111)
cell culture
(2)
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Human
(107)
Mouse
(2)
Rat
(2)
Bovine
(1)
Porcine
(1)
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Showing Publications 1 - 10 of 117. Show All 117 Publications.
Publications using 5036-WN Applications Species
S Miura, A Suzuki Generation of Mouse and Human Organoid-Forming Intestinal Progenitor Cells by Direct Lineage Reprogramming Cell Stem Cell, 2017;0(0):. 2017 [PMID: 28943029] (Bioassay, Human) Bioassay Human
N Yucer, M Holzapfel, T Jenkins Vo, L Lenaeus, L Ornelas, A Laury, D Sareen, R Barrett, BY Karlan, CN Svendsen Directed Differentiation of Human Induced Pluripotent Stem Cells into Fallopian Tube Epithelium Sci Rep, 2017;7(1):10741. 2017 [PMID: 28878359] (Bioassay, Human) Bioassay Human
X Li, C Wang, G Zhang, M Liang, B Zhang AKAP2 is upregulated in ovarian cancer, and promotes growth and migration of cancer cells Mol Med Rep, 2017;0(0):. 2017 [PMID: 28849175] (Bioassay, Human) Bioassay Human
S Premarathn, M Murtaza, N Matigian, LA Jolly, SA Wood Loss of Usp9x disrupts cell adhesion, and components of the Wnt and Notch signaling pathways in neural progenitors Sci Rep, 2017;7(1):8109. 2017 [PMID: 28808228] (Bioassay, Human) Bioassay Human
Y Kishimoto, B Ohkawara, T Sakai, M Ito, A Masuda, N Ishiguro, C Shukunami, D Docheva, K Ohno Wnt/?-catenin signaling suppresses expressions of Scx, Mkx, and Tnmd in tendon-derived cells PLoS ONE, 2017;12(7):e0182051. 2017 [PMID: 28750046] (Bioassay, Human) Bioassay Human
S Monteagudo, FMF Cornelis, C Aznar-Lope, P Yibmantasi, LA Guns, P Carmeliet, F Cailotto, RJ Lories DOT1L safeguards cartilage homeostasis and protects against osteoarthritis Nat Commun, 2017;8(0):15889. 2017 [PMID: 28627522] (Bioassay, Human) Bioassay Human
JG Camp, K Sekine, T Gerber, H Loeffler-W, H Binder, M Gac, S Kanton, J Kageyama, G Damm, D Seehofer, L Belicova, M Bickle, R Barsacchi, R Okuda, E Yoshizawa, M Kimura, H Ayabe, H Taniguchi, T Takebe, B Treutlein Multilineage communication regulates human liver bud development from pluripotency Nature, 2017;0(0):. 2017 [PMID: 28614297] (Bioassay, Human) Bioassay Human
S Zeng, AM Seifert, JQ Zhang, MJ Cavnar, TS Kim, VP Balachandr, JA Santamaria, NA Cohen, MJ Beckman, B Medina, F Rossi, MH Crawley, JK Loo, JH Maltbaek, P Besmer, CR Antonescu, RP DeMatteo Wnt/?-catenin signaling contributes to tumor malignancy and is targetable in gastrointestinal stromal tumor Mol. Cancer Ther., 2017;0(0):. 2017 [PMID: 28611108] (Bioassay, Human) Bioassay Human
ML Xu, CWC Bi, EYL Liu, TTX Dong, KWK Tsim Wnt3a Induces the Expression of Acetylcholinesterase during Osteoblast Differentiation via the Runx2 Transcription Factor J. Biol. Chem., 2017;0(0):. 2017 [PMID: 28607150] (Bioassay, Rat) Bioassay Rat
XH Jiang, YT Xie, YP Cai, J Ren, T Ma Effects of hepatitis C virus core protein and nonstructural protein 4B on the Wnt/?-catenin pathway BMC Microbiol., 2017;17(1):124. 2017 [PMID: 28545480] (Bioassay, Human) Bioassay Human
Show All 117 Publications.

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Bioinformatics

Gene Symbol WNT3A
Entrez
Uniprot