Human Von Willebrand Factor ELISA Kit (Colorimetric)

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ELISA: Human Von Willebrand Factor ELISA Kit (Colorimetric) [KA0512] - Von Willebrand Factor ELISA Kit [KA0512] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, NULL
Suitable Sample Type
Cell Culture Samples, Plasma, Serum
Standard Curve Range
0.625 to 40 mIU/mL
Sensitivity
0.38 mIU/mL

Order Details

Human Von Willebrand Factor ELISA Kit (Colorimetric) Summary

Description
VWF (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human VWF.
Standard Curve Range
0.625 to 40 mIU/mL
Sensitivity
0.38 mIU/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 7.0%
Intra-Assay
CV% < 5.0%
Spike Recovery
86-117%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
VWF

Applications/Dilutions

Dilutions
  • ELISA
  • Quantification
Publications
Read Publications using KA0512.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Human VWF Antibody (50x): A 50-fold concentrated biotinylated polyclonalantibody against VWF.
  2. Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substratetetramethylbenzidine.
  3. Human VWF Microplate: A 96-well polystyrene microplate coated with a monoclonalantibody against VWF.
  4. Human VWF Standard: Human VWF in a buffered protein base (lyophilized), calibratedagainst WHO 1st International Standard.
  5. MIX Diluent Concentrate (10x): A 10-fold concentrated buffered protein base.
  6. Sealing Tapes: Precut, pressure sensitive sealing tapes that can be cut to fit the format ofthe individual assay.
  7. Stop Solution: A 0.5 N hydrochloric acid to stop the chromogen substrate reaction.
  8. Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate
  9. Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human Von Willebrand Factor ELISA Kit (Colorimetric)

  • coagulation factor VIII VWF
  • F8
  • F8VWF
  • von Willebrand factor
  • VWD
  • vWF

Background

The glycoprotein encoded by this gene functions as both an antihemophilic factor carrier and a platelet-vessel wall mediator in the blood coagulation system. It is crucial to the hemostasis process. Mutations in this gene or deficiencies in this protein result in von Willebrand's disease. An unprocessed pseudogene has been found on chromosome 22.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for Von Willebrand Factor ELISA Kit (KA0512)(6)

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Product General Protocols

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Thrombomodulin, also known as BDCA-3, is a glycosylated transmembrane protein present on the surface of vascular endothelial cells. Thrombomodulin is a high-affinity receptor for thrombin, a key protein in the coagulation cascade. Formation of the...  Read full blog post.

Von Willebrand Factor: An important mediator and carrier of hemostasis
Human Von Willebrand Factor (factor VIII R: Ag) is a 270 kDa multimeric plasma gylcoprotein. Important in the maintenance of hemostasis, it mediates platelet adhesion to injured vessel walls and serves as a carrier and stabilizer for coagulation facto...  Read full blog post.

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Bioinformatics

Gene Symbol VWF