Human Von Willebrand Factor ELISA Kit (Colorimetric)

ELISA: Human Von Willebrand Factor ELISA Kit (Colorimetric) [KA0512] - Von Willebrand Factor ELISA Kit [KA0512] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.

• Reactivity: Hu
• Applications: ELISA, NULL
• Suitable Sample Type: Cell Culture Samples, Plasma, Serum
• Standard Curve Range: 0.625 to 40 mIU/mL
• Sensitivity: 0.38 mIU/mL

Human Von Willebrand Factor ELISA Kit (Colorimetric) Summary

• Description: VWF (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human VWF.
• Standard Curve Range: 0.625 to 40 mIU/mL
• Sensitivity: 0.38 mIU/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 7.0%
• Intra-Assay: CV% < 5.0%
• Spike Recovery: 86-117%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: VWF

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human Von Willebrand Factor ELISA Kit (Colorimetric)

• coagulation factor VIII VWF
• F8
• F8VWF
• von Willebrand factor
• VWD
• vWF

Background

The glycoprotein encoded by this gene functions as both an antihemophilic factor carrier and a platelet-vessel wall mediator in the blood coagulation system. It is crucial to the hemostasis process. Mutations in this gene or deficiencies in this protein result in von Willebrand's disease. An unprocessed pseudogene has been found on chromosome 22.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Von Willebrand Factor ELISA Kit (KA0512)(6)

Publications using KA0512

• Jee A, Eun C, You J et al. Characterization of the von Willebrand factor/factor VIII complex produced by a novel purification process. Arch Pharm Res. 2020-07-14 [PMID: 32666302]
• Schoner A, Tyrrell C, Wu M et al. Endocardial Endothelial Dysfunction Progressively Disrupts Initially Anti then Pro-Thrombotic Pathways in Heart Failure Mice. PLoS One. 2015-11-13 [PMID: 26565707]
• Choi JS, Kwak KA, Park MJ et al. Ratio of angiopoietin-2 to angiopoietin-1 predicts mortality in acute lung injury induced by paraquat. Med Sci Monit. 2013-01-01 [PMID: 23302768] (Human)
• Pittet JL, Barbalat V, Sanvert M et al. Evaluation of a new automated ELISA test for von Willebrand factor using two monoclonal antibodies. Blood Coagul Fibrinolysis. 1997-06-01 [PMID: 9199817]
• Inward CD, Pall AA, Adu D et al. Soluble circulating cell adhesion molecules in haemolytic uraemic syndrome. Pediatr Nephrol. 1995-10-01 [PMID: 8580013]
• Morton LF, Griffin B, Pepper DS, Barnes MJ. The interaction between collagens and factor VIII/von Willebrand factor: investigation of the structural requirements for interaction. Thromb Res. 1983-12-15 [PMID: 6420928]

FAQs for Von Willebrand Factor ELISA Kit (KA0512). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: VWF