Von Willebrand Factor Antibody (21-43)

Images

 
Western Blot: Von Willebrand Factor Antibody (21-43) [NB100-65194] - Human Placenta lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-von Willebrand Factor (1:1000 dilution). ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, RIA
Clone
21-43
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.4 mg/ml

Order Details

Von Willebrand Factor Antibody (21-43) Summary

Immunogen
Purified Human Factor VIII complex
Marker
Endothelial Cell Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
VWF
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Radioimmunoassay 1:100-1:2000
Application Notes
This antibody reacts strongly with human von Willebrand Factor or Factor VIII Related Antigen in both plasma, using RIA or ELISA, and in tissues by immunofluorescence or immunoperoxidase staining. The antibody is suitable for use with frozen and formalin fixed tissue sections. Detecta a band at ~310 by Western Blot.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 0.2% BSA and 50% (v/v) Glycerol
Preservative
0.1% Sodium Azide
Concentration
0.4 mg/ml
Purity
Protein A purified

Alternate Names for Von Willebrand Factor Antibody (21-43)

  • coagulation factor VIII VWF
  • F8
  • F8VWF
  • von Willebrand factor
  • VWD
  • vWF

Background

von Willebrand Factor is synthesized by endothelial cells, causing adhesion of platelets to injured vessel walls and functions as a carrier and stabilizer for coagulation of Factor VIII. It is one of the most widely used endothelial cell markers for studying angiogenesis / neovascularization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Von Willebrand Factor Antibody (NB100-65194) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for Von Willebrand Factor Antibody (NB100-65194)

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Thrombomodulin, also known as BDCA-3, is a glycosylated transmembrane protein present on the surface of vascular endothelial cells. Thrombomodulin is a high-affinity receptor for thrombin, a key protein in the coagulation cascade. Formation of the...  Read full blog post.

Von Willebrand Factor: An important mediator and carrier of hemostasis
Human Von Willebrand Factor (factor VIII R: Ag) is a 270 kDa multimeric plasma gylcoprotein. Important in the maintenance of hemostasis, it mediates platelet adhesion to injured vessel walls and serves as a carrier and stabilizer for coagulation facto...  Read full blog post.

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Bioinformatics

Gene Symbol VWF
Entrez
Uniprot