Cullin 2 Antibody

Western Blot: Cullin 2 Antibody [NBP1-49873] - Analysis of Cullin 2 in NIH3T3 lysate (35ug protein in RIPA buffer) using NBP1-49873 at 1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Cullin 2 Antibody [NBP1-49873] - 3.75ug/ml staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Cullin 2 Antibody Summary

• Immunogen: Peptide with sequence C-QYIERSQASADE corresponding to C-Terminus according to NP_003582.2.
• Epitope: QYIERSQASADE - C Terminus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CUL2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-3ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1:1000
• Application Notes: Western blot: Approx 100kDa band observed in lysates of cell line NIH3T3 (calculated MW of 87.0kDa according to Human NP_003582.2 and of 86.9kDa according to Mouse NP_083678.2). Primary incubation was 1 hour
  IHC: Paraffin embedded Human Breast.

Reactivity Notes

Expected from sequence similarity: Rat, Canine, Bovine

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cullin 2 Antibody

• CUL2
• CUL-2
• Cullin 2
• cullin-2
• MGC131970

Background

Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of Cdc53 from yeast, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1 mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3 facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin- like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, or vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cullin 2 Antibody (NBP1-49873)(1)

Publication using NBP1-49873

• Argyropoulos G, Stutz AM, Ilnytska O et al. KIF5B gene sequence variation and response of cardiac stroke volume to regular exercise. Physiol Genomics 2009-01-08 [PMID: 18984674]

Bioinformatics

• Gene Symbol: CUL2
• Entrez:
  • 8453(Human)
  • 71745(Mouse)
  • 361258(Rat)
• Uniprot:
  • NP_003582.2(Human)