Vinculin Antibody (VCL/2573)

Images

 
Immunohistochemistry-Paraffin: Vinculin Antibody (VCL/2573) [NBP2-79807] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Vinculin Antibody (VCL/2573).
Protein Array: Vinculin Antibody (VCL/2573) [NBP2-79807] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Vinculin Antibody (VCL/2573) Z- and S- Score: The Z-score represents the ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications IHC, IHC-P, PA
Clone
VCL/2573
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Vinculin Antibody (VCL/2573) Summary

Immunogen
Recombinant fragment (around aa 174-322) of human Vinculin protein (exact sequence is proprietary)
Localization
Cytoplasmic
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
VCL
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Vinculin Antibody (VCL/2573)

  • CMD1W
  • CMH15
  • metavinculin
  • MVCL
  • VCL
  • Vinculin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Vinculin Antibody (NBP2-79807). (Showing 1 - 1 of 1 FAQ).

  1. Do you have any 100 coda or bigger controls?
    • I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-79941AF350
Alexa Fluor 405 NBP2-79941AF405
Alexa Fluor 488 NBP2-79941AF488
Alexa Fluor 532 NBP2-79941AF532
Alexa Fluor 594 NBP2-79941AF594
Alexa Fluor 647 NBP2-79941AF647
Alexa Fluor 700 NBP2-79941AF700
Alexa Fluor 750 NBP2-79941AF750
Biotin NBP2-79941B
DyLight 350 NBP2-79941UV
DyLight 405 NBP2-79941V
DyLight 488 NBP2-79941G
DyLight 550 NBP2-79941R
DyLight 594 NBP2-79941DL594
DyLight 650 NBP2-79941C
DyLight 680 NBP2-79941FR
DyLight 755 NBP2-79941IR
FITC NBP2-79941F
HRP NBP2-79941H
Janelia Fluor 549 NBP2-79941JF549
Janelia Fluor 646 NBP2-79941JF646

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Bioinformatics Tool for Vinculin Antibody (NBP2-79807)

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Bioinformatics

Gene Symbol VCL
Uniprot