Vinculin Antibody (728513) [DyLight 350]

• Reactivity: Hu, Mu, Rt
• Applications: IHC, ICC/IF
• Clone: 728513
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 350

Vinculin Antibody (728513) [DyLight 350] Summary

• Immunogen: E. coli-derived recombinant human Vinculin
  Lys1020-Gln1134
  Accession # P18206
• Specificity: Detects human Vinculin in direct ELISAs.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: VCL
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Vinculin Antibody (728513) [DyLight 350]

• CMD1W
• CMH15
• metavinculin
• MVCL
• VCL
• Vinculin

Background

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Vinculin Antibody (FAB68961D). (Showing 1 - 1 of 1 FAQ).

1. Do you have any 100 coda or bigger controls?
   • I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.

Bioinformatics

• Gene Symbol: VCL