Vinculin Antibody (728513) [Alexa Fluor® 594]

• Applications: IHC, ICC/IF
• Clone: 728513
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

Vinculin Antibody (728513) [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human Vinculin
  Lys1020-Gln1134
  Accession # P18206
• Specificity: Detects human Vinculin in direct ELISAs.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

VCL (vinculin) is a 117 kDa actin binding cytoskeletal phosphoprotein found in adherens-type cell-cell and cell-matrix junctions. The 1066 amino acid (aa) human vinculin contains an N-terminal globular head (aa 2-835) with a talin interaction domain (aa 168-208) and three tandem repeats (aa 259-589), a pro-rich linker region (aa 826-878), and a C-terminal tail that facilitates phospholipid membrane insertion (aa 1003-1134). An 1134 aa form termed metavinculin contains 68 aa inserted after aa 915 and mainly occurs in cardiac, skeletal and smooth muscle. Human, mouse and rat vinculin share 100% aa sequence identity within the region used as an immunogen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Vinculin Antibody (FAB68961T). (Showing 1 - 1 of 1 FAQ).

1. Do you have any 100 coda or bigger controls?
   • I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.