Vimentin Antibody [Unconjugated]

Images

 
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, MEF mouse embryonic feeder cells, RAW 264.7 mouse monocyte/macrophage cell line, NR8383 rat alveolar macrophage cell line, and ...read more
Vimentin was detected in immersion fixed paraffin-embedded sections of human skin using 10 µg/mL Goat Anti-Human Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) overnight at ...read more
Vimentin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) at 1.7 µg/mL for 3 hours at ...read more
Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Vimentin at ...read more
Genetic Strategies: Vimentin was detected in immersion fixed K562 human chronic myelogenous leukemia cell line but is not detected in Vimentin knockout (KO) K562 Human Cell Line cell line using Goat ...read more
Western blot shows lysates of K562 human chronic myelogenous leukemia parental cell line and Vimentin knockout K562 cell line (KO). PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse/Rat Vimentin Antigen ...read more
TGF-beta 1, -beta 2 and -beta 3 reduce lymphatic marker expression in LECs.Primary human LECs were treated with 10, 20 or 30 ng/ml TGF-beta 1, -beta 2 and -beta 3 for 72 hours (a) or 100 hours (b). Untreated cells ...read more
Extracellular vesicles impact multiple signaling elements of the glioma stem cell program.a Mass spectrometry quantification (n = 3) for the presence of MMPs in HUVEC-EVs (EEVs), proneural-GSC157 cells, GSC157 cells ...read more
NRF1 and/or E2 re-programming contributed to the overexpression of pluripotency and epithelial-mesenchymal transition (EMT) markers and differentiation of tumor initiating breast cancer cells to other-types of cells. ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Vimentin Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Vimentin
Ser2-Glu466
Accession # P08670
Specificity
Detects human, mouse, and rat Vimentin in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
VIM
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Knockout Validated 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 2 ug/mL
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF2105 in the following applications:

Publications
Read Publications using
AF2105 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Vimentin Antibody [Unconjugated]

  • epididymis secretory sperm binding protein
  • FLJ36605
  • VIM
  • Vimentin

Background

Vimentin is a 57 kDa class III intermediate filament (IF) protein that belongs to the intermediate filament family. It is the predominant IF in cells of mesenchymal origin such as vascular endothelium and blood cells (1‑3). The human Vimentin cDNA encodes a 466 amino acid (aa) protein that contains head and tail regions with multiple regulatory Ser/Thr phosphorylation sites, and a central rod domain with three coiled-coil regions separated by linkers (1, 2). Human Vimentin shares 97‑98% aa identity with mouse, rat, ovine, bovine and canine Vimentin. Sixteen Vimentin coiled-coil dimers self-assemble to form intermediate (10‑12 nm wide) filaments (4). These filaments then anneal longitudinally to form non-polarized fibers that support cell structure and withstand stress (4). IF fibers are highly dynamic, and half-life depends on the balance between kinase and phosphatase activity. For example, phosphorylation followed by dephosphorylation drives IF disintegration, followed by reorganization during mitosis (1, 5, 6). Interactions of head and tail domains link IFs with other structures such as actin and microtubule cytoskeletons (7). Vimentin is involved in positioning autophagosomes, lysosomes and the Golgi complex within the cell (8). It facilitates cell migration and motility by recycling internalized trailing edge integrins back to the cell surface at the leading edge (9‑11). Vimentin helps maintain the lipid composition of cellular membranes, and caspase cleavage of Vimentin is a key event in apoptosis (8, 12). Phosphorylation promotes secretion of Vimentin by TNF-alpha -stimulated macrophages (13). Extracellular Vimentin has been shown to associate with several microbes, and appears to promote an antimicrobial oxidative burst (13, 14). Cell-associated Vimentin can also interact with NKp46 to recruit NK cells to tuberculosis-infected monocytes (15).

  1. Omary, M.B. et al. (2006) Trends Biochem. Sci. 31:383.
  2. Ivaska, J. et al. (2007) Exp. Cell Res. 313:2050.
  3. Ferrari, S. et al. (1986) Mol. Cell. Biol. 6:3614.
  4. Sokolova, A.V. et al. (2006) Proc. Natl. Acad. Sci. USA 103:16206.
  5. Eriksson, J.E. et al. (2004) J. Cell Sci. 117:919.
  6. Li, Q.-F. et al. (2006) J. Biol. Chem. 281:34716.
  7. Esue, O. et al. (2006) J. Biol. Chem. 281:30393.
  8. Styers, M.L. et al. (2005) Traffic 6:359.
  9. McInroy, L. and A. Maata (2007) Biochem. Biophys. Res. Commun. 360:109.
  10. Nieminen, M. et al. (2006) Nat. Cell Biol. 8:156.
  11. Ivaska, J. et al. (2005) EMBO J. 24:3834.
  12. Byun, Y. et al. (2001) Cell Death Differ. 8:443.
  13. Mor-Vaknin, N. et al. (2003) Nat. Cell Biol. 5:59.
  14. Zou, Y. et al. (2006) Biochem. Biophys. Res. Commun. 351:625.
  15. Garg, A. et al. (2006) J. Immunol. 177:6192.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Vimentin Antibody (AF2105)(33)

We have publications tested in 3 confirmed species: Human, Mouse, Xenograft.

We have publications tested in 8 applications: Binding Assay, ELISA Development, Flow Cytometry, ICC, IHC, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
Binding Assay
(1)
ELISA Development
(2)
Flow Cytometry
(1)
ICC
(9)
IHC
(7)
IHC-P
(1)
Immunoprecipitation
(2)
Western Blot
(6)
All Applications
Filter By Species
Human
(23)
Mouse
(2)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using AF2105 Applications Species
Windus, LCE;Matigian, N;Avery, VM; Induction of Reactive Bone Stromal Fibroblasts in 3D Models of Prostate Cancer Bone Metastases Biology 2023-06-15 [PMID: 37372146] (ICC, Western Blot, Human) ICC, Western Blot Human
Kumar, T;Nee, K;Wei, R;He, S;Nguyen, QH;Bai, S;Blake, K;Gong, Y;Pein, M;Sei, E;Hu, M;Casasent, A;Thennavan, A;Li, J;Tran, T;Chen, K;Nilges, B;Kashikar, N;Braubach, O;Cheikh, BB;Nikulina, N;Chen, H;Teshome, M;Menegaz, B;Javaid, H;Nagi, C;Montalvan, J;Tifrea, DF;Edwards, R;Lin, E;Parajuli, R;Winocour, S;Thompson, A;Lim, B;Lawson, DA;Kessenbrock, K;Navin, N; A spatially resolved single cell genomic atlas of the adult human breast bioRxiv : the preprint server for biology 2023-04-25 [PMID: 37163043] (IHC, Human) IHC Human
I Mizdrak, M Mizdrak, A Racetin, B Boškovi?, B Benzon, MG Durdov, K Vukojevi?, N Filipovi? Expression of Connexins 37, 40 and 45, Pannexin 1 and Vimentin in Laryngeal Squamous Cell Carcinomas Genes, 2023-02-09;14(2):. 2023-02-09 [PMID: 36833374] (IHC, Human) IHC Human
J Sun, S Osenberg, A Irwin, LH Ma, N Lee, Y Xiang, F Li, YW Wan, IH Park, M Maletic-Sa, N Ballas Mutations in the transcriptional regulator MeCP2 severely impact key cellular and molecular signatures of human astrocytes during maturation Cell Reports, 2023-01-05;42(1):111942. 2023-01-05 [PMID: 36640327] (IHC, Human) IHC Human
R Chimienti, T Baccega, S Torchio, F Manenti, S Pellegrini, A Cospito, A Amabile, MT Lombardo, P Monti, V Sordi, A Lombardo, M Malnati, L Piemonti Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement Cell Reports, 2022-09-27;40(13):111423. 2022-09-27 [PMID: 36170817] (ICC, Human) ICC Human
L Adnani, J Kassouf, B Meehan, C Spinelli, N Tawil, I Nakano, J Rak Angiocrine extracellular vesicles impose mesenchymal reprogramming upon proneural glioma stem cells Nature Communications, 2022-09-19;13(1):5494. 2022-09-19 [PMID: 36123372] (Western Blot, Human) Western Blot Human
Y Weng, H Wang, D Wu, S Xu, X Chen, J Huang, Y Feng, L Li, Z Wang A novel lineage of osteoprogenitor cells with dual epithelial and mesenchymal properties govern maxillofacial bone homeostasis and regeneration after MSFL Cell Research, 2022-07-12;0(0):. 2022-07-12 [PMID: 35821090] (IHC, Mouse) IHC Mouse
Earl Parker Scott, Emma Breyak, Ryuichi Nishinakamura, Yasushi Nakagawa The zinc finger transcription factor Sall1 is required for the early developmental transition of microglia in mouse embryos Glia 2022-09-01 [PMID: 35567352]
Qianhui Yu, Umut Kilik, Emily M. Holloway, Yu-Hwai Tsai, Christoph Harmel, Angeline Wu et al. Charting human development using a multi-endodermal organ atlas and organoid models Cell 2021-06-10 [PMID: 34019796]
AHM Ng, P Khoshakhla, JE Rojo Arias, G Pasquini, K Wang, A Swiersy, SL Shipman, E Appleton, K Kiaee, RE Kohman, A Vernet, M Dysart, K Leeper, W Saylor, JY Huang, A Graveline, J Taipale, DE Hill, M Vidal, JM Melero-Mar, V Busskamp, GM Church A comprehensive library of human transcription factors for cell fate engineering Nature Biotechnology, 2020-11-30;0(0):. 2020-11-30 [PMID: 33257861] (Flow Cytometry, Human) Flow Cytometry Human
Show All 33 Publications.

Reviews for Vimentin Antibody (AF2105) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using AF2105:
Filter by Applications
WB
(1)
IF
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot Vimentin AF2105
Enlarge
5
reviewed by:
Yongkang Yang
WB Human 02/20/2021
View

Summary

ApplicationWestern Blot
Sample TestedBreast cancer cells
SpeciesHuman
LotKRZ101921
  4
reviewed by:
Verified Customer
IF Human 01/06/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23658023
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Vimentin Antibody (AF2105) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Vimentin Products

Blogs on Vimentin. Showing 1-10 of 14 blog posts - Show all blog posts.

Taking Biomarker Discovery From 2D to 3D: Increased Biological Activity of EVs Isolated From 3D Prostate Cancer Cultures
Jamshed Arslan, Pharm D, PhD Tissues within the human body are made of a three-dimensional (3D) arrangement of cells working together to perform vital functions. The commonly used 2D monolayer cultures have limited ...  Read full blog post.


  Read full blog post.

Antibody treatment can generate microglia-like cells from bone marrow
By Jennifer Sokolowski, MD, PhD.Microglia play important roles in the brain in both homeostatic and pathological conditions, acting to clear debris and dying cells. There is evidence to suggest that microglial dys...  Read full blog post.

Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

The use of actin as a loading control in research on fruiting-body development and vegetative growth in Sordaria macrospora research
Sordaria macrospora is a filamentous fungus that serves as very useful system for scientific research due to a short life cycle and easy manipulation.  Just like any other model organism, it is important to have an effective loading control to va...  Read full blog post.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

CD63: is it pro-metastatic or anti-metastatic?
CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.

Vimentin: Regulating EMT and Cancer
Vimentin, a member of the intermediate filament (IF) family, is a protein responsible for maintaining cellular integrity and reducing damage caused by stress. The vimentin protein is ubiquitously expressed in normal mesenchymal cells, and recent resea...  Read full blog post.

Showing 1-10 of 14 blog posts - Show all blog posts.
mFluor Violet Conjugated Antibodies

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Yongkang Yang
02/20/2021
Application: WB
Species: Human

 
Verified Customer
01/06/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol VIM
Uniprot