Vimentin Antibody (RV203) - BSA Free

Immunohistochemistry: Vimentin Antibody (RV203) [NBP1-97671] - BTIC markers in our CNS-PNET model tumors.G: Vimentin 5X, insert- 5X digital (LCAS-R-12 weeks post-injection) H: Vimentin 20X, insert- 5X digital (LC26-R-12 weeks post-injection) TU-tumor; P-parenchyma; V-ventricle; CP-choroid plexus. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0173106), licensed under a CC-BY licence.

Immunohistochemistry-Frozen: Vimentin Antibody (RV203) [NBP1-97671] - Frozen section of human colon showing positive staining in connective tissue cells and no reactivity in epithelial cells

Flow Cytometry: Vimentin Antibody (RV203) [NBP1-97671] - An intracellular stain was performed on HeLa cells with NBP1-97671PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.

Immunohistochemistry-Paraffin: Vimentin Antibody (RV203) [NBP1-97671] - Paraffin section of human colon

Immunohistochemistry-Frozen: Vimentin Antibody (RV203) [NBP1-97671] - Figure 3. Immunohistochemistry with NBP1-97671 (RV203) on a frozen section of human kidney showing positive staining in blood vessels, glomeruli and connective tissue cells and no reactivity in epithelial cells.

• Reactivity: Hu, Mu, Rt, Po, Ca, Gt, Ha
• Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
• Clone: RV203
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Vimentin Antibody (RV203) - BSA Free Summary

• Immunogen: This antibody is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a cytoskeletal vimentin extract of calf lens.
• Marker: Mesenchymal Cells Marker
• Specificity: This antibody reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: VIM
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100-1:200
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:100-1:200
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:1000
• Application Notes: Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
  
  This antibody is CyTOF ready.
• Theoretical MW: 53.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28249000).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.2
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Notes

The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Alternate Names for Vimentin Antibody (RV203) - BSA Free

• epididymis secretory sperm binding protein
• FLJ36605
• VIM
• Vimentin

Background

Vimentin is a type III intermediate filament (IF) protein, encoded by the VIM gene, that plays an essential role in cytoskeletal function and is largely expressed by mesenchymal cell such as endothelial cells, fibroblasts, and immune cells (1). Human vimentin monomer protein is 466 amino acids (aa) in length with a theoretical molecular weight of ~54 kDa (2). Vimentin protein contains a central rod domain composed of four alpha-helices which is flanked by an N-terminal head domain and C-terminal tail domain (1,3). Vimentin monomers dimerize to form more complex structures resulting in the assembly of eight tetramers, referred to as unit-length filaments, which then assemble into full-length intermediate filaments that are 10-12 nm wide (1,3,4).

Activated macrophages have been shown to secrete phosphorylated vimentin which can be stimulated by a variety of pathophysiological factors including oxidized low-density lipoproteins and TNF-alpha or inhibited by IL-10 (1). The vimentin protein is often expressed at the cell surface playing a role in cell-cell interactions, tissue damage and repair, immune response, and pathogen recognition (1). Vimentin functions in many cytoskeletal processes including cell migration, which is highlighted by its upregulation during epithelial-to-mesenchymal transition (EMT) (4,5). Vimentin is a commonly used marker for EMT and is expressed by many tumor types (5). For example, high metastasis of oral squamous cell carcinomas also showed high vimentin positive expression in immunohistochemical staining analysis (5). A number of vimentin targeting compounds are in cancer-related clinical trials, however, given the multifunctional role of vimentin, the effect of inhibition on non-malignant cells needs to be thoroughly examined (5).

References

1. Ramos, I., Stamatakis, K., Oeste, C. L., & Perez-Sala, D. (2020). Vimentin as a Multifaceted Player and Potential Therapeutic Target in Viral Infections. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms21134675

2. Uniprot (P08670)

3. Morrow, C. S., & Moore, D. L. (2020). Vimentin's side gig: Regulating cellular proteostasis in mammalian systems. Cytoskeleton (Hoboken, N.J.). https://doi.org/10.1002/cm.21645

4. van Bodegraven, E. J., & Etienne-Manneville, S. (2020). Intermediate filaments against actomyosin: the david and goliath of cell migration. Current Opinion in Cell Biology. https://doi.org/10.1016/j.ceb.2020.05.006

5. Strouhalova, K., Prechova, M., Gandalovicova, A., Brabek, J., Gregor, M., & Rosel, D. (2020). Vimentin Intermediate Filaments as Potential Target for Cancer Treatment. Cancers. https://doi.org/10.3390/cancers12010184

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Vimentin Antibody (NBP1-97671)(4)

Publications using NBP1-97671

• Kutryb-Zajac B, Jablonska P, Serocki M et al Extracellular Adenine Nucleotide and Adenosine Metabolism in Calcific Aortic Valve Disease. BioRxiv 2019 Aug 28 (ICC/IF, Flow, Human)
  
  Details:
  Citation using the Alexa Fluor 488 version of this antibody.
• Niu W, Lim T, Alshihri A et al. Platelet-Derived Growth Factor Stimulated Migration of Bone Marrow Mesenchymal Stem Cells into an Injectable Gelatin-Hydroxyphenyl Propionic Acid Matrix Biomedicines Feb 17 2021 [PMID: 33671438]
• Malchenko S, Sredni S, Boyineni J, Bi Y. Characterization of brain tumor initiating cells isolated from an animal model of CNS primitive neuroectodermal tumors. Oncotarget. 2018 Feb 09 [PMID: 29568390] (IHC-P, Mouse)
• Malchenko S, Sredni ST, Bi Y et al. Stabilization of HIF-1a and HIF-2a, up-regulation of MYCC and accumulation of stabilized p53 constitute hallmarks of CNS-PNET animal model. PLoS ONE. Mar 1 2017 [PMID: 28249000] (IHC, Mouse)

Bioinformatics

• Gene Symbol: VIM