Villin 1 Antibody - BSA Free

Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-32841] - Rat colon. Villin stained by Villin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: Villin 1 Antibody [NBP1-32841] - A. 50 ug rat kidney lysate/extract 7.5 % SDS-PAGE Villin antibody dilution: 1:2000

Western Blot: Villin 1 Antibody [NBP1-32841] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membranes were blotted with Villin antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Villin 1 Antibody [NBP1-32841] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Villin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Villin 1 Antibody [NBP1-32841] - Analysis of methanol-fixed HeLa, using Villin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.

Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-32841] - Paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-32841] - Mouse duodenum. Villin stained by Villin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-32841] - Rat kidney. Villin stained by Villin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-32841] - Dog intestine.VIL1 stained by Villin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-32841] - Cat colon.VIL1 stained by Villin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: Villin 1 Antibody [NBP1-32841] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Villin 1 antibody (NBP1-32841) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-32841] - Villin 1 antibody detects Villin 1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.Villin 1 stained by Villin 1 antibody (NBP1-32841) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-32841] - Villin 1 antibody detects VIL1 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded dog colon.VIL1 stained by Villin 1 antibody (NBP1-32841) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-32841] - Villin 1 antibody detects Villin 1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse duodenum.Villin 1 stained by Villin 1 antibody (NBP1-32841) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: Villin 1 Antibody [NBP1-32841] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Villin antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Mu, Rt, Ca, Fe, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Villin 1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Villin 1 Antibody - BSA Free (NBP1-32841) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Villin 1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human Villin 1. The exact sequence is proprietary.
• Localization: Cytoplasm, cytoskeleton
• Predicted Species: Bovine (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VIL1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen Assay dependent
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
• Theoretical MW: 93 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cat (100%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Villin 1 Antibody - BSA Free

• D2S1471
• VIL
• villin 1
• villin-1

Background

This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Villin 1 Antibody (NBP1-32841)(1)

Publication using NBP1-32841

• Frede A, Czarnewski P, Monasterio G et al. B cell expansion hinders the stroma-epithelium regenerative cross talk during mucosal healing Immunity 2022-12-13 [PMID: 36462502]

Bioinformatics

• Gene Symbol: VIL1