Villin 1 Antibody

Images

 
Western Blot: Villin 1 Antibody [NBP1-32841] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membranes were blotted with Villin antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect ...read more
Immunocytochemistry/ Immunofluorescence: Villin 1 Antibody [NBP1-32841] - Villin 1 and Cadherin-17 were detected in immersion fixed, paraffin-embedded sections of MimEX adult stem cell-derived ascending colon tissue ...read more
Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-32841] - Paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.
Western Blot: Villin 1 Antibody [NBP1-32841] - Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE; antibody diluted at 1:5000.
Western Blot: Villin 1 Antibody [NBP1-32841] - A. 50 ug mouse kidney lysate/extract 7.5 % SDS-PAGEVillin antibody dilution: 1:1000
Western Blot: Villin 1 Antibody [NBP1-32841] - A. 50 ug rat kidney lysate/extract 7.5 % SDS-PAGE Villin antibody dilution: 1:2000
Immunocytochemistry/ Immunofluorescence: Villin 1 Antibody [NBP1-32841] - Analysis of methanol-fixed HeLa, using Villin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
 

Independent Antibodies

       

Order Details

Villin 1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human Villin. The exact sequence is proprietary.
Localization
Cytoplasm, cytoskeleton
Predicted Species
Bovine (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VIL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Villin 1 Antibody

  • D2S1471
  • VIL
  • villin 1
  • villin-1

Background

This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Calculators

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Bioinformatics

Gene Symbol VIL1