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FAM3D Antibody

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
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AF3027-SP
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FAM3D Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse FAM3D
Trp39-Met224
Accession # P97805
Specificity
Detects mouse FAM3D in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human (rh) FAM3D is observed and less than 1% cross reactivity with rhFAM3A, rhFAM3B and rhFAM3C is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FAM3D
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF3027 in the following application:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FAM3D Antibody

  • cytokine-like protein EF-7
  • EF7
  • FAM3D
  • family with sequence similarity 3, member D
  • OIT1

Background

FAM3D, also known as oncoprotein induced protein 1 and protein EF-7, is a secreted protein belonging to the FAM3 family of novel four-helix-bundle cytokine-like proteins. FAM3D is highly expressed in the testis and placenta. The amino acid sequence of mature mouse FAM3D is 74%, 88% and 75% identical to that of human, rat and canine FAM3D, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-EpCAM/TROP1 (AUA1) conjugated to Alexa Fluor 488 [NB600-1182AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective.
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POMGNT1
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POMGNT1 [Unconjugated]
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Immunocytochemistry/Immunofluorescence: FAM84A Antibody [NBP2-14006] - Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: FAM84A Antibody [NBP2-14006] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
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FAM3D Antibody
AF3027
FAM3D Antibody
Species: Mu
Applications: WB

Publications for FAM3D Antibody (AF3027) (0)

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Review for FAM3D Antibody (AF3027) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF3027:
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  4
reviewed by:
Anonymous
 ICC Mouse 01/07/2015
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Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 22226334
SpeciesMouse

Blocking

Blocking DetailsSee PMID 22226334

Details

Detection NotesSee PMID 22226334

Comments

CommentsPublished: See PMID 22226334Independent review from antibodyresource.com

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 Anonymous
01/07/2015
Application: ICC
Species: Mouse
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Bioinformatics

Gene Symbol FAM3D
Uniprot