CHRND Antibody (88B)

Images

 
Immunocytochemistry/ Immunofluorescence: CHRND Antibody (88B) [NB120-2804] - Analysis of Nicotinic Acetylcholine Receptor using Anti-Nicotinic Acetylcholine Receptor Monoclonal Antibody (88B) shows staining in U251 ...read more
Immunohistochemistry-Paraffin: CHRND Antibody (88B) [NB120-2804] - Normal biopsies of deparaffinized Mouse lymph node tissue.
Immunocytochemistry/ Immunofluorescence: CHRND Antibody (88B) [NB120-2804] - Analysis of Nicotinic Acetylcholine Receptor using Anti-Nicotinic Acetylcholine Receptor Monoclonal Antibody (88B) shows staining in Hela ...read more
Immunocytochemistry/ Immunofluorescence: CHRND Antibody (88B) [NB120-2804] - Analysis of Nicotinic Acetylcholine Receptor using Anti-Nicotinic Acetylcholine Receptor Monoclonal Antibody (88B) shows staining in Neuro-2a ...read more
Immunohistochemistry-Paraffin: CHRND Antibody (88B) [NB120-2804] - Normal biopsies of deparaffinized Mouse brain tissue.
Immunohistochemistry-Paraffin: CHRND Antibody (88B) [NB120-2804] - Normal biopsies of deparaffinized Mouse kidney tissue.

Product Details

Summary
Reactivity Hu, Mu, Rt, Am, Ch, FiSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
88B
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CHRND Antibody (88B) Summary

Immunogen
Purified Torpedo californica acetylcholine receptor.
Specificity
Detects nicotinic acetylcholine receptor (nAChR) gamma and delta subunits in torpedo and the delta subunit in mouse, rat, frog and chicken. Does not detect the alpha 1 and beta 1 subunits.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CHRND
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • Immunocytochemistry/Immunofluorescence 1:20 - 100
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen 1:1000
  • Immunohistochemistry-Paraffin 1:1000
  • Immunoprecipitation 1:10 - 1:500
Application Notes
WB: Detects an approx. 60 kDa protein representing the gamma subunit and a 65 kDa protein representing the delta subunit from torpedo skeletal muscle homogenates. Under non-reducing conditions the delta subunit migrates mostly as a dimer of ~130 kDa. IHC: Staining of nAChR in rat skeletal muscle results in strong staining of the motor endplate. Cryostat or permeabilization of the sections is recommended due to the reactivity of this antibody with the cytoplasmic side of the receptor.
Publications
Read Publications using
NB120-2804 in the following applications:

  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for CHRND Antibody (88B)

  • acetylcholine receptor subunit delta
  • ACHRD
  • ACHRDSCCMS
  • cholinergic receptor, nicotinic, delta polypeptide
  • cholinergic receptor, nicotinic, delta
  • CHRND
  • CMS2A
  • FCCMS
  • nAChR delta
  • Nicotinic Acetylcholine R delta

Background

Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction. The channel-linked AChR that mediates rapid, excitatory actions of acetylcholine is called nicotinic AChR (nAChR) because it can be activated by nicotine. The non-channel linked AChR that medicates the slow actions of acetylcholine, which can be either inhibitory or excitatory, is called muscarinic AChR (mAChR) because it can be activated by muscarine. The nAChRs fall into two subclasses, the muscle-type and the neuronal-type. The muscle-type receptor is a pentameric glycoprotein of five membrane spanning subunits, (2 alpha 1's, beta 1, gamma or epsilon and delta) which form a ligand gated ion channel. The neuronal nAChR is also a pentamer, but unlike the muscle-type, is made up of only two general types of subunits, alpha and beta of which multiple subtypes have been described (alpha 2 to alpha 8 and beta 2 to beta 4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-79952
Species: Hu
Applications: WB
NBP1-85537
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-57262
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-91258
Species: Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-20900
Species: Hu
Applications: WB, PEP-ELISA
NBP2-75707
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AF7197
Species: Hu, Mu
Applications: WB, IHC, ICC
MAB4540
Species: Hu, Mu, Rt
Applications: WB, IHC
NBP1-87752
Species: Hu
Applications: IHC, IHC-P
NB300-131
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, KO
NBP1-47804
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, KO
MAB7500
Species: Hu
Applications: WB, ICC
NBP1-33177
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-02073
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-79951
Species: Hu
Applications: WB
NBP2-61674
Species: Hu
Applications: WB, ELISA
NB600-594
Species: Hu, Mu, Rt, Bv, Fe
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NB120-2804
Species: Hu, Mu, Rt, Am, Ch, Fi
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for CHRND Antibody (NB120-2804)(4)

Reviews for CHRND Antibody (NB120-2804) (0)

There are no reviews for CHRND Antibody (NB120-2804). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CHRND Antibody (NB120-2804) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional CHRND Products

Bioinformatics Tool for CHRND Antibody (NB120-2804)

Discover related pathways, diseases and genes to CHRND Antibody (NB120-2804). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CHRND Antibody (NB120-2804)

Discover more about diseases related to CHRND Antibody (NB120-2804).
 

Pathways for CHRND Antibody (NB120-2804)

View related products by pathway.

PTMs for CHRND Antibody (NB120-2804)

Learn more about PTMs related to CHRND Antibody (NB120-2804).

Blogs on CHRND

There are no specific blogs for CHRND, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CHRND Antibody (88B) and receive a gift card or discount.

Bioinformatics

Gene Symbol CHRND
Entrez
Uniprot