Nebulin Antibody (NB2)

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Immunohistochemistry: Nebulin Antibody (NB2) [NB120-11099] - Frozen sections, acetone fixed Chicken cardiac muscle were stained with 1:400 Monoclonal Anti-Nebulin antibody produced in Mouse, Clone: NB2. followed by 1:40 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Al, Am, Av, Bv, Ch, Fi, RbSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
NB2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Nebulin Antibody (NB2) Summary

Immunogen
Full length native protein (purified) (Chicken).
Localization
Cytoplasmic
Specificity
Localizes nebulin in skeletal muscle of a wide variety of species from cold-blooded vertebrates to man. Does not crossreact with smooth or cardiac muscle, non-muscle tissue and cultured cells. Localizes nebulin within the I band of skeletal muscle sections by indirect immunofluorescence.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NEB
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:400
  • Immunohistochemistry
  • Western Blot 1:100-1:2000
Application Notes
IF: Use at a dilution of 1/400. WB: Use at an assay dependent dilution. Detects a band of approximately 500 kDa. By indirect immunofluorescence, the antibody shows a typical striated staining pattern of frozen sections of chicken skeletal muscle. It stains within the I-band and shows a decoration line at a distance of 0.22 um from the z-line in electron micrographs. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Publications
Read Publication using
NB120-11099 in the following applications:

  • WB
    1 publication

Reactivity Notes

Cross-reacts with a wide range of species, including salamander, pigeon, alligator, toad. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
15mM Sodium Azide
Purity
Unpurified

Alternate Names for Nebulin Antibody (NB2)

  • DKFZp686C1456
  • FLJ11505
  • FLJ36536
  • FLJ39568
  • FLJ39584
  • NEB177D
  • nebulin
  • NEM2

Background

Nebulin is a high molecular weight protein (500-800 kD), localized at the N2-line of skeletal muscle myofibrils. It exhibits an elastic stretch dependence, as it maintains the same proportional distance between the z-line and the m-line as the sarcomere is stretched.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nebulin Antibody (NB120-11099)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NEB
Entrez