Nebulin Antibody (NB2)

Immunohistochemistry: Nebulin Antibody (NB2) [NB120-11099] - Frozen sections, acetone fixed Chicken cardiac muscle were stained with 1:400 Monoclonal Anti-Nebulin antibody produced in Mouse, Clone: NB2. followed by 1:40 Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat.

• Reactivity: Hu, Mu, Rt, Po, Al, Am, Av, Bv, Ch, Fi, Rb
• Applications: WB, ICC/IF, IHC
• Clone: NB2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Nebulin Antibody (NB2) Summary

• Immunogen: Full length native protein (purified) (Chicken).
• Localization: Cytoplasmic
• Specificity: Localizes nebulin in skeletal muscle of a wide variety of species from cold-blooded vertebrates to man. Does not crossreact with smooth or cardiac muscle, non-muscle tissue and cultured cells. Localizes nebulin within the I band of skeletal muscle sections by indirect immunofluorescence.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NEB
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:400
  • Immunohistochemistry
  • Western Blot 1:100-1:2000
• Application Notes: IF: Use at a dilution of 1/400. WB: Use at an assay dependent dilution. Detects a band of approximately 500 kDa. By indirect immunofluorescence, the antibody shows a typical striated staining pattern of frozen sections of chicken skeletal muscle. It stains within the I-band and shows a decoration line at a distance of 0.22 um from the z-line in electron micrographs. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with a wide range of species, including salamander, pigeon, alligator, toad. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 15mM Sodium Azide
• Purity: Unpurified

Alternate Names for Nebulin Antibody (NB2)

• DKFZp686C1456
• FLJ11505
• FLJ36536
• FLJ39568
• FLJ39584
• NEB177D
• nebulin
• NEM2

Background

Nebulin is a high molecular weight protein (500-800 kD), localized at the N2-line of skeletal muscle myofibrils. It exhibits an elastic stretch dependence, as it maintains the same proportional distance between the z-line and the m-line as the sarcomere is stretched.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nebulin Antibody (NB120-11099)(1)

Publication using NB120-11099

• Kesherwani V, Shahshahan HR, Mishra PK. Cardiac transcriptome profiling of diabetic Akita mice using microarray and next generation sequencing PLoS ONE 2017-08-24 [PMID: 28837672] (WB, Mouse)

Bioinformatics

• Gene Symbol: NEB
• Entrez:
  • 4703(Human)
  • 17996(Mouse)
  • 311029(Rat)