Villin 1 Antibody (VIL1/8105R) - Azide and BSA Free

Formalin-fixed, paraffin-embedded human colon adenocarcinoma stained with Villin Recombinant Rabbit Monoclonal Antibody (VIL1/8105R). Inset: PBS instead of primary antibody; secondary only negative control.

• Reactivity: Hu
• Applications: IHC
• Clone: VIL1/8105R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Villin 1 Antibody (VIL1/8105R) - Azide and BSA Free Summary

• Description: Positive Controls: A549, HepG2 or HCT116 cells. Human colon or rectum.
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment (around aa600-700) of human Villin 1 protein (exact sequence is proprietary)
• Localization: Cytoplasm. Cell Surface.
• Specificity: Anti-Villin 1 labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract This antibody also labels Merkel cells of the skin
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: VIL1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Villin 1 Antibody (VIL1/8105R) - Azide and BSA Free

• D2S1471
• VIL
• villin 1
• villin-1

Background

Villin 1 encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VIL1