VIAAT/SLC32A1/VGAT Antibody (CL2793) - BSA Free

Immunohistochemistry: VIAAT/SLC32A1/VGAT Antibody (2793) [NBP2-46628] - Staining of rat basal forebrain shows strong positivity in GABAergic fibers in the globus pallidus.

Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody (2793) [NBP2-46628] - Staining of human midbrain shows positivity in GABAergic fibers in substantia nigra, pars reticulata.

Immunohistochemistry: VIAAT/SLC32A1/VGAT Antibody (2793) [NBP2-46628] - Staining of mouse brain shows moderate positivity in GABAergic processes in the cerebellum.

Immunohistochemistry: VIAAT/SLC32A1/VGAT Antibody (2793) [NBP2-46628] - Staining of mouse basal forebrain shows positivity in GABAergic fibers in the globus pallidus.

Immunohistochemistry: VIAAT/SLC32A1/VGAT Antibody (2793) [NBP2-46628] - Staining of rat midbrain shows strong positivity in GABAergic fibers in substantia nigra, pars reticulata.

• Reactivity: Hu, Mu, Rt
• Applications: IHC
• Clone: CL2793
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

VIAAT/SLC32A1/VGAT Antibody (CL2793) - BSA Free Summary

• Description: Novus Biologicals Mouse VIAAT/SLC32A1/VGAT Antibody (CL2793) - BSA Free (NBP2-46628) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from Q9H598, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC32A1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  VIAAT/SLC32A1/VGAT Recombinant Protein Antigen (NBP2-46628PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for VIAAT/SLC32A1/VGAT Antibody (CL2793) - BSA Free

• bA122O1.1
• hVIAAT
• SLC32A1
• solute carrier family 32 (GABA vesicular transporter), member 1
• Solute carrier family 32 member 1
• Vesicular GABA transporter
• vesicular inhibitory amino acid transporter
• VGAT
• VGATGABA and glycine transporter
• VIAAT

Background

The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles(McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Acid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC32A1
• Entrez:
  • 140679(Human)
• Uniprot:
  • Q9H598(Human)