Gephyrin/GPHN Antibody (OTI3B6)

Images

 
Western Blot: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Gephyrin monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Gephyrin.
Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Staining of paraffin-embedded Human prostate tissue using anti-Gephyrin mouse monoclonal antibody.
Flow Cytometry: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Gephyrin antibody, and ...read more
Western Blot: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Gephyrin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of ...read more
Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Gephyrin mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Gephyrin mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Staining of paraffin-embedded Human liver tissue using anti-Gephyrin mouse monoclonal antibody.
Flow Cytometry: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Analysis of Jurkat cells, using anti-Gephyrin antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, MkSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI3B6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Gephyrin/GPHN Antibody (OTI3B6) Summary

Immunogen
Full length human recombinant protein of human GPHN (NP_065857) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
GPHN
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
Theoretical MW
83.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-03449 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Affinity purified

Alternate Names for Gephyrin/GPHN Antibody (OTI3B6)

  • GEPH
  • Gephyrin
  • GPH
  • GPHN
  • GPHRYN
  • KIAA1385GPHGEPH

Background

This gene encodes a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency. Numerous alternatively spliced transcript variants encoding different isoforms have been described; however, the full-length nature of all transcript variants is not currently known. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Gephyrin/GPHN Antibody (NBP2-03449) (0)

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Review for Gephyrin/GPHN Antibody (NBP2-03449) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-03449:
Filter by Applications
IF
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Fugui Li
IF Mouse 10/06/2014
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Summary

ApplicationImmunofluorescence
Sample Testedmouse brain sections
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Gephyrin/GPHN Antibody (NBP2-03449) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-71556AF350
Alexa Fluor 405 NBP2-71556AF405
Alexa Fluor 488 NBP2-71556AF488
Alexa Fluor 532 NBP2-71556AF532
Alexa Fluor 594 NBP2-71556AF594
Alexa Fluor 647 NBP2-71556AF647
Alexa Fluor 700 NBP2-71556AF700
Alexa Fluor 750 NBP2-71556AF750
Allophycocyanin NBP2-71556APC
Biotin NBP2-71556B
DyLight 350 NBP2-71556UV
DyLight 405 NBP2-71556V
DyLight 488 NBP2-71556G
DyLight 550 NBP2-71556R
DyLight 594 NBP2-71556DL594
DyLight 650 NBP2-71556C
DyLight 680 NBP2-71556FR
DyLight 755 NBP2-71556IR
FITC NBP2-71556F
HRP NBP2-71556H
Janelia Fluor 549 NBP2-71556JF549
Janelia Fluor 646 NBP2-71556JF646
PE NBP2-71556PE
PerCP NBP2-71556PCP

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Bioinformatics Tool for Gephyrin/GPHN Antibody (NBP2-03449)

Discover related pathways, diseases and genes to Gephyrin/GPHN Antibody (NBP2-03449). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Gephyrin/GPHN Antibody (NBP2-03449)

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Pathways for Gephyrin/GPHN Antibody (NBP2-03449)

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Recent Reviews

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Fugui Li
10/06/2014
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol GPHN
Entrez
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