VIAAT/SLC32A1/VGAT Antibody (CL2793) - Azide and BSA Free

Staining of human midbrain shows positivity in GABAergic fibers in substantia nigra, pars reticulata.

Staining of rat midbrain shows strong positivity in GABAergic fibers in substantia nigra, pars reticulata.

Staining of mouse brain shows moderate positivity in GABAergic processes in the cerebellum.

Staining of mouse basal forebrain shows positivity in GABAergic fibers in the globus pallidus.

Staining of rat basal forebrain shows strong positivity in GABAergic fibers in the globus pallidus.

• Reactivity: Hu, Mu, Rt
• Applications: IHC
• Clone: CL2793
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

VIAAT/SLC32A1/VGAT Antibody (CL2793) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse VIAAT/SLC32A1/VGAT Antibody (CL2793) - Azide and BSA Free (NBP2-46628) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of Q9H598, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC32A1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for VIAAT/SLC32A1/VGAT Antibody (CL2793) - Azide and BSA Free

• bA122O1.1
• hVIAAT
• SLC32A1
• solute carrier family 32 (GABA vesicular transporter), member 1
• Solute carrier family 32 member 1
• Vesicular GABA transporter
• vesicular inhibitory amino acid transporter
• VGAT
• VGATGABA and glycine transporter
• VIAAT

Background

The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles(McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Acid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC32A1