VGLUT1 Antibody (S28-9) [Alexa Fluor® 405]

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC
• Clone: S28-9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

VGLUT1 Antibody (S28-9) [Alexa Fluor® 405] Summary

• Immunogen: Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1
• Localization: Cytoplasmic Vesicle , Secretory Vesicle , Synaptic Vesicle Membrane , Membrane , Cell Junction , Synapse
• Specificity: Detects 52kDa. No cross-reactivity against VGlut2.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC17A7
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for VGLUT1 Antibody (S28-9) [Alexa Fluor® 405]

• BNPI
• BNPIbrain-specific Na-dependent inorganic phosphate cotransporter
• Brain-specific Na(+)-dependent inorganic phosphate cotransporter
• member 7
• SLC17A7
• solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter)
• Solute carrier family 17 member 7
• VGLUT1
• VGLUT1vesicular glutamate transporter 1

Background

VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate. Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for VGLUT1 Antibody (NBP2-59329AF405) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer Func Mouse 07/19/2021

Summary

• Application: Functional Assay
• Sample Tested: Adult mouse brain tissue,Mouse brain
• Species: Mouse
• Lot: 1205-060721-AF405

Comments

• Comments: Single cell suspensions were prepared from mouse brain from 1 year old 5xFAD mice using enzymatic digestion and a 27% percoll gradient to clear myelin. Cells were then stained for CD45 and CD11b, fixed and permeabilized, and then stained with the ant-vglut1 antibody for 30 minutes at room temperature. ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

Bioinformatics

• Gene Symbol: SLC17A7