Versican Antibody (S351-23) - BSA Free

Western Blot: Versican Antibody (S351-23) [NBP2-22408] - Western Blot analysis of Rat Brain Membrane and brain showing detection of 350kDa Versicanprotein using Mouse Anti-Versican Monoclonal Antibody, Clone S351-23 (NBP2-22408). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane and brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Versican Monoclonal Antibody (NBP2-22408) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: KPL TMB. Predicted/Observed Size: 350kDa. Other Band(s): Multiple other bands.

Immunocytochemistry/Immunofluorescence: Versican Antibody (S351-23) [NBP2-22408] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VersicanMonoclonal Antibody, Clone S351-23 (NBP2-22408). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VersicanMonoclonal Antibody (NBP2-22408) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VersicanAntibody (D) Composite.

Immunocytochemistry/Immunofluorescence: Versican Antibody (S351-23) [NBP2-22408] - Analysis using Mouse Anti-Versican Monoclonal Antibody, Clone S351-23. Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Versican Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Membrane, Extracellular Space, Extracellular Matrix. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Versican Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence: Versican Antibody (S351-23) [NBP2-22408] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VersicanMonoclonal Antibody, Clone S351-23 (NBP2-22408). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VersicanMonoclonal Antibody (NBP2-22408) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Membrane, Extracellular Space, Extracellular Matrix. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VersicanAntibody. (D) Composite.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: S351-23
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Versican Antibody (S351-23) - BSA Free Summary

• Description: Novus Biologicals Mouse Versican Antibody (S351-23) - BSA Free (NBP2-22408) is a monoclonal antibody validated for use in WB and ICC/IF. Anti-Versican Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Fusion protein amino acids 362-585 (glycosaminoglycan alpha domain) of mouse Versican core protein
• Localization: Extracellular Space , Extracellular Matrix
• Specificity: Detects >350kDa.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: VCAN
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000
• Application Notes: 1 ug/ml of Versican Antibody was sufficient for detection of Versican in 20 ug of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Versican Antibody (S351-23) - BSA Free

• Chondroitin sulfate proteoglycan 2WGN
• Chondroitin sulfate proteoglycan core protein 2
• CSPG2
• CSPG2DKFZp686K06110
• ERVR
• GHAP
• Glial hyaluronate-binding protein
• Large fibroblast proteoglycan
• PG-M
• PG-MWGN1
• VCAN
• versican core protein
• versican proteoglycan
• Versican

Background

Versican is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Versican Antibody (NBP2-22408)(2)

Publications using NBP2-22408

• Hanuman S, B HK, Pai KSR et Al. Surface-Conjugated Galactose on Electrospun Polycaprolactone Nanofibers: An Innovative Scaffold for Uterine Tissue Engineering ACS Omega 2024-08-13 [PMID: 39157094]
• Liu Z, Huang J, Kang D et al. Microenvironmental reprogramming of human dermal papilla cells for hair follicle tissue engineering Acta biomaterialia 2022-11-05 [PMID: 36347448] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: VCAN
• Uniprot:
  • Q62059(Mouse)