ADAMTS1 Antibody


Western blot shows lysates of P19 mouse embryonal carcinoma cell line and OVCAR‑3 human ovarian carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse ADAMTS1 Antigen Affinity-purified more
ADAMTS1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Human/Mouse ADAMTS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5867) at 15 µg/mL overnight at 4 °C. more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IP

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ADAMTS1 Antibody Summary

Chinese hamster ovary cell line CHO-derived recombinant mouse ADAMTS1
Accession # P97857
Detects human and mouse ADAMTS1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) ADAMTS4 and rhADAMTS15 is observed and less than 1% cross‑reactivity with rhADAMTSL1.2, rhADAMTSL2, rhADAMTS5, rhADAMTS8, rhADAMTS10, rhADAMTS12, rhADAMTS13, and rhADAMTS16 is observed.
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL
Read Publications using
AF5867 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAMTS1 Antibody

  • a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 1
  • ADAM metallopeptidase with thrombospondin type 1 motif, 1
  • ADAM-TS 1
  • ADAM-TS1
  • ADAMTS-1
  • C3-C5
  • METH1
  • METH-1


ADAMTS1 (a disintegrin and metalloproteinase with thrombospondin motifs 1), also known as METH1, is the founding member of the family of secreted zinc proteases with a multidomain structure (1-3). The protein precursor consists of a signal peptide and the following domains: pro, catalytic, disintegrinlike, TS type 1 motif, cysteine rich, spacer and a variable number of thrombospondin type 1 motifs. Based on their substrate specificity, ADAMTS1 and associated family members may be key enzymes in the degradation of cartilage leading to inflammation and arthritis (4). It is an active protease cleaving alpha 2macroglobulin (5), aggrecan (6), and versican (7). Compared to ADAMTS4 (aggrecanase 1) and ADAMTS5 (aggrecanase 2), the aggrecanase activity of ADAMTS1 is lower. However, its activity can be enhanced by the binding of a cofactor such as fibulin1 (8). ADAMTS1 is essential for normal growth and the structure and function of the kidneys, adrenal glands and female reproductive organs (9). It also plays an important role in atherosclerosis (10). It has been shown to inhibit endothelial cell proliferation by direct binding and sequestration of VEGF165 and to inhibit fibroblast migration at high concentrations by binding to FGF 2 (11, 12). The purified rmADAMTS1 starts at the N terminus of the catalytic domain and ends in the Cys-rich domain.
  1. Vazquez, F. et al. (1999) J. Biol. Chem. 274:23349.
  2. Kuno, K. et al. (1997) J. Biol. Chem. 272:556.
  3. Porter, S. et al. (2005) Biochem. J. 386:15.
  4. Nagase, H. and M. Kashiwagi (2003) Arthritis Res. Ther. 5:94.
  5. Kuno, K. et al. (1999) J. Biol. Chem. 274:18821.
  6. Kuno, K. et al. (2000) FEBS Lett. 478:241.
  7. Russel, D. L. et al. (2003) J. Biol. Chem. 278:42330.
  8. Lee, N. et al. (2005) J. Biol. Chem. 280:34796.
  9. Shindo, T. et al. (2000) J. Clin. Invest. 105:1345.
  10. Wight, T.N. (2005) Arterioscler Thromb. Vasc. Biol. 25:12.
  11. Luque, A. et al. (2003) J. Biol. Chem. 278:23656.
  12. Krampert, M. et al. (2005) J. Biol. Chem. 280:23844.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ADAMTS1 Antibody (AF5867)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC, Western Blot.

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Showing Publications 1 - 6 of 6.
Publications using AF5867 Applications Species
SY Cheung, M Sayeed, K Nakuluri, L Li, BJ Feldman MYH9 facilitates autoregulation of adipose tissue depot development JCI Insight, 2021;6(9):. 2021 [PMID: 33986190] (Western Blot, Mouse) Western Blot Mouse
C Peris-Torr, MDC Plaza-Calo, R López-Domí, S Domínguez-, A Barrientos, P Carmona-Sá, JC Rodríguez- Extracellular Protease ADAMTS1 Is Required at Early Stages of Human Uveal Melanoma Development by Inducing Stemness and Endothelial-Like Features on Tumor Cells Cancers (Basel), 2020;12(4):. 2020 [PMID: 32230715] (Western Blot, Human) Western Blot Human
E Vorkapic, M Folkesson, K Magnell, M Bohlooly-Y, T Länne, D Wågsäter ADAMTS-1 in abdominal aortic aneurysm PLoS ONE, 2017;12(6):e0178729. 2017 [PMID: 28570682] (Western Blot, Human) Western Blot Human
Wong J, Krueger K, Costa M, Aggarwal A, Du H, McLaughlin T, Feldman B A glucocorticoid- and diet-responsive pathway toggles adipocyte precursor cell activity in vivo. Sci Signal, 2016;9(451):ra103. 2016 [PMID: 27811141] (Western Blot, Mouse) Western Blot Mouse
Tian , E, Stevens , Sharon R, Guan , Yu, Springer , Danielle, Anderson , Stasia A, Starost , Matthew, Patel , Vyomesh, Ten Hagen , Kelly G, Tabak , Lawrence Galnt1 is required for normal heart valve development and cardiac function. PLoS ONE, 2015;10(1):e0115861. 2015 [PMID: 25615642] (IHC, Mouse) IHC Mouse
Inagaki J, Takahashi K, Ogawa H, Asano K, Faruk Hatipoglu O, Cilek M, Obika M, Ohtsuki T, Hofmann M, Kusachi S, Ninomiya Y, Hirohata S ADAMTS1 inhibits lymphangiogenesis by attenuating phosphorylation of the lymphatic endothelial cell-specific VEGF receptor. Exp Cell Res, 2014;323(2):263-75. 2014 [PMID: 24631293] (Western Blot, Human) Western Blot Human

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Biotin BAF5867

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Gene Symbol ADAMTS1