ADAMTS1 Antibody

Western blot shows lysates of P19 mouse embryonal carcinoma cell line and OVCAR‑3 human ovarian carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse ADAMTS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5867) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ADAMTS1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

ADAMTS1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Human/Mouse ADAMTS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5867) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cartilage primordium; dorsal ganglia cell bodies and processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

ADAMTS1 Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse ADAMTS1
  Phe254-Cys725
  Accession # P97857
• Specificity: Detects human and mouse ADAMTS1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) ADAMTS4 and rhADAMTS15 is observed and less than 1% cross‑reactivity with rhADAMTSL1.2, rhADAMTSL2, rhADAMTS5, rhADAMTS8, rhADAMTS10, rhADAMTS12, rhADAMTS13, and rhADAMTS16 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ADAMTS1
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAMTS1 Antibody

• a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 1
• ADAM metallopeptidase with thrombospondin type 1 motif, 1
• ADAM-TS 1
• ADAMTS1
• ADAM-TS1
• ADAMTS-1
• C3-C5
• METH1
• METH-1

Background

ADAMTS1 (a disintegrin and metalloproteinase with thrombospondin motifs 1), also known as METH1, is the founding member of the family of secreted zinc proteases with a multidomain structure (1-3). The protein precursor consists of a signal peptide and the following domains: pro, catalytic, disintegrinlike, TS type 1 motif, cysteine rich, spacer and a variable number of thrombospondin type 1 motifs. Based on their substrate specificity, ADAMTS1 and associated family members may be key enzymes in the degradation of cartilage leading to inflammation and arthritis (4). It is an active protease cleaving alpha 2macroglobulin (5), aggrecan (6), and versican (7). Compared to ADAMTS4 (aggrecanase 1) and ADAMTS5 (aggrecanase 2), the aggrecanase activity of ADAMTS1 is lower. However, its activity can be enhanced by the binding of a cofactor such as fibulin1 (8). ADAMTS1 is essential for normal growth and the structure and function of the kidneys, adrenal glands and female reproductive organs (9). It also plays an important role in atherosclerosis (10). It has been shown to inhibit endothelial cell proliferation by direct binding and sequestration of VEGF165 and to inhibit fibroblast migration at high concentrations by binding to FGF 2 (11, 12). The purified rmADAMTS1 starts at the N terminus of the catalytic domain and ends in the Cys-rich domain.

1. Vazquez, F. et al. (1999) J. Biol. Chem. 274:23349.
2. Kuno, K. et al. (1997) J. Biol. Chem. 272:556.
3. Porter, S. et al. (2005) Biochem. J. 386:15.
4. Nagase, H. and M. Kashiwagi (2003) Arthritis Res. Ther. 5:94.
5. Kuno, K. et al. (1999) J. Biol. Chem. 274:18821.
6. Kuno, K. et al. (2000) FEBS Lett. 478:241.
7. Russel, D. L. et al. (2003) J. Biol. Chem. 278:42330.
8. Lee, N. et al. (2005) J. Biol. Chem. 280:34796.
9. Shindo, T. et al. (2000) J. Clin. Invest. 105:1345.
10. Wight, T.N. (2005) Arterioscler Thromb. Vasc. Biol. 25:12.
11. Luque, A. et al. (2003) J. Biol. Chem. 278:23656.
12. Krampert, M. et al. (2005) J. Biol. Chem. 280:23844.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ADAMTS1 Antibody (AF5867)(6)

Publications using AF5867

• SY Cheung, M Sayeed, K Nakuluri, L Li, BJ Feldman MYH9 facilitates autoregulation of adipose tissue depot development JCI Insight, 2021;6(9):. 2021 [PMID: 33986190] (Western Blot, Mouse)
• C Peris-Torr, MDC Plaza-Calo, R López-Domí, S Domínguez-, A Barrientos, P Carmona-Sá, JC Rodríguez- Extracellular Protease ADAMTS1 Is Required at Early Stages of Human Uveal Melanoma Development by Inducing Stemness and Endothelial-Like Features on Tumor Cells Cancers (Basel), 2020;12(4):. 2020 [PMID: 32230715] (Western Blot, Human)
• E Vorkapic, M Folkesson, K Magnell, M Bohlooly-Y, T Länne, D Wågsäter ADAMTS-1 in abdominal aortic aneurysm PLoS ONE, 2017;12(6):e0178729. 2017 [PMID: 28570682] (Western Blot, Human)
• Wong J, Krueger K, Costa M, Aggarwal A, Du H, McLaughlin T, Feldman B A glucocorticoid- and diet-responsive pathway toggles adipocyte precursor cell activity in vivo. Sci Signal, 2016;9(451):ra103. 2016 [PMID: 27811141] (Western Blot, Mouse)
• Tian , E, Stevens , Sharon R, Guan , Yu, Springer , Danielle, Anderson , Stasia A, Starost , Matthew, Patel , Vyomesh, Ten Hagen , Kelly G, Tabak , Lawrence Galnt1 is required for normal heart valve development and cardiac function. PLoS ONE, 2015;10(1):e0115861. 2015 [PMID: 25615642] (IHC, Mouse)
• Inagaki J, Takahashi K, Ogawa H, Asano K, Faruk Hatipoglu O, Cilek M, Obika M, Ohtsuki T, Hofmann M, Kusachi S, Ninomiya Y, Hirohata S ADAMTS1 inhibits lymphangiogenesis by attenuating phosphorylation of the lymphatic endothelial cell-specific VEGF receptor. Exp Cell Res, 2014;323(2):263-75. 2014 [PMID: 24631293] (Western Blot, Human)

Bioinformatics

• Gene Symbol: ADAMTS1
• Uniprot:
  • P97857()