DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the astrocytes showing cytoplasmic staining. Protocol available in COMET™ Panel Builder." alt="Versican was detected in immersion fixed paraffin-embedded sections of human brain Glioblastoma using Rabbit Anti-Human Versican Monoclonal Antibody (Catalog # NBP1-85432) at 10ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the astrocytes showing cytoplasmic staining. Protocol available in COMET™ Panel Builder." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85432-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22408-0.025mg | |||
NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="Versican was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 10 µg/mL Goat Anti-Human Versican Isoform V0 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3054) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3054-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3054-SP | |||
DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # 
DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # 
DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # ![Immunohistochemistry-Paraffin: Versican Antibody [NBP1-85432] - Staining of human uterine cervix shows strong positivity in the extracellular matrix.](http://images.novusbio.com/fullsize/Versican-Antibody-Immunohistochemistry-Paraffin-NBP1-85432-img0015.jpg)
![Immunohistochemistry-Paraffin: Versican Antibody [NBP1-85432] - Staining of human cerebral cortex shows strong positivity in neuropil.](http://images.novusbio.com/fullsize/Versican-Antibody-Immunohistochemistry-Paraffin-NBP1-85432-img0012.jpg)
![Immunohistochemistry-Paraffin: Versican Antibody [NBP1-85432] - Staining of human stomach shows moderate positivity in extracellular matrix.](http://images.novusbio.com/fullsize/Versican-Antibody-Immunohistochemistry-Paraffin-NBP1-85432-img0013.jpg)
![Immunohistochemistry-Paraffin: Versican Antibody [NBP1-85432] - Staining of human tonsil shows no positivity as expected.](http://images.novusbio.com/fullsize/Versican-Antibody-Immunohistochemistry-Paraffin-NBP1-85432-img0014.jpg)
![Immunohistochemistry: Versican Antibody [NBP1-85432] - Immunohistochemical staining of stromal protein expression in DCIS with sclerotic or myxoid stromaMicrophotographs displaying HE staining (A-B), & IHC staining for biglycan (C-D), decorin (E-F) & versican (G-H). Panels A-C-E-G display photographs of one DCIS lesion with sclerotic stroma; panels B-D-F-H display one DCIS lesion with myxoid stroma. This figure illustrates that myxoid DCIS present reduced periductal decorin staining & tend to have increased periductal versican & biglycan expression, whereas sclerotic DCIS generally present strong stromal decorin immunoreactivity, & tend to lack stromal versican & biglycan. Original magnification 100x. Image collected & cropped by CiteAb from the following publication (//www.oncoscience.us/lookup/doi/10.18632/oncoscience.87), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-85432_rabbit-polyclonal-versican-antibody-31020241533496.jpg)
![Immunohistochemistry: Versican Antibody [NBP1-85432] - Immunohistochemical staining of stromal protein expression in DCIS with sclerotic or myxoid stromaMicrophotographs displaying HE staining (A-B), & IHC staining for biglycan (C-D), decorin (E-F) & versican (G-H). Panels A-C-E-G display photographs of one DCIS lesion with sclerotic stroma; panels B-D-F-H display one DCIS lesion with myxoid stroma. This figure illustrates that myxoid DCIS present reduced periductal decorin staining & tend to have increased periductal versican & biglycan expression, whereas sclerotic DCIS generally present strong stromal decorin immunoreactivity, & tend to lack stromal versican & biglycan. Original magnification 100x. Image collected & cropped by CiteAb from the following publication (//www.oncoscience.us/lookup/doi/10.18632/oncoscience.87), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-85432_rabbit-polyclonal-versican-antibody-310202415304243.jpg)
![Western Blot: Versican Antibody (S351-23) [NBP2-22408] - Western Blot analysis of Rat Brain Membrane and brain showing detection of 350kDa Versicanprotein using Mouse Anti-Versican Monoclonal Antibody, Clone S351-23 (NBP2-22408). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane and brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Versican Monoclonal Antibody (NBP2-22408) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: KPL TMB. Predicted/Observed Size: 350kDa. Other Band(s): Multiple other bands.](http://images.novusbio.com/fullsize/Versican-Antibody-S351-23-Western-Blot-NBP2-22408-img0004.jpg "Western Blot: Versican Antibody (S351-23) [NBP2-22408] - Western Blot analysis of Rat Brain Membrane and brain showing detection of 350kDa Versicanprotein using Mouse Anti-Versican Monoclonal Antibody, Clone S351-23 (NBP2-22408). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane and brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Versican Monoclonal Antibody (NBP2-22408) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: KPL TMB. Predicted/Observed Size: 350kDa. Other Band(s): Multiple other bands.")
![Immunocytochemistry/Immunofluorescence: Versican Antibody (S351-23) [NBP2-22408] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VersicanMonoclonal Antibody, Clone S351-23 (NBP2-22408). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VersicanMonoclonal Antibody (NBP2-22408) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VersicanAntibody (D) Composite.](http://images.novusbio.com/fullsize/Versican-Antibody-S351-23-Immunocytochemistry-Immunofluorescence-NBP2-22408-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Versican Antibody (S351-23) [NBP2-22408] - Analysis using Mouse Anti-Versican Monoclonal Antibody, Clone S351-23. Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Versican Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Membrane, Extracellular Space, Extracellular Matrix. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Versican Antibody (D) Composite.](http://images.novusbio.com/fullsize/Versican-Antibody-S351-23-Immunocytochemistry-Immunofluorescence-NBP2-22408-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Versican Antibody (S351-23) [NBP2-22408] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VersicanMonoclonal Antibody, Clone S351-23 (NBP2-22408). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VersicanMonoclonal Antibody (NBP2-22408) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Membrane, Extracellular Space, Extracellular Matrix. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VersicanAntibody. (D) Composite.](http://images.novusbio.com/fullsize/Versican-Antibody-S351-23-Immunocytochemistry-Immunofluorescence-NBP2-22408-img0006.jpg)
NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />
![Western Blot: Versican Antibody (6D8K9) [NBP3-15841] - Analysis of extracts of various cell lines, using Versican antibody (NBP3-15841) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Versican-Antibody-6D8K9-Western-Blot-NBP3-15841-img0007.jpg "Western Blot: Versican Antibody (6D8K9) [NBP3-15841] - Analysis of extracts of various cell lines, using Versican antibody (NBP3-15841) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunocytochemistry/ Immunofluorescence: Versican Antibody (6D8K9) [NBP3-15841] - Immunofluorescence analysis of NIH-3T3 cells using Versican Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15841_rabbit-versican-mab-6d8k9-72202512443356.jpg)
![Immunohistochemistry-Paraffin: Versican Antibody (6D8K9) [NBP3-15841] - Mouse brain using Versican Rabbit mAb (NBP3-15841) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Versican-Antibody-6D8K9-Immunohistochemistry-Paraffin-NBP3-15841-img0003.jpg)
![Immunohistochemistry-Paraffin: Versican Antibody (6D8K9) [NBP3-15841] - Rat kidney using Versican Rabbit mAb (NBP3-15841) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Versican-Antibody-6D8K9-Immunohistochemistry-Paraffin-NBP3-15841-img0001.jpg)
![Immunohistochemistry-Paraffin: Versican Antibody (6D8K9) [NBP3-15841] - Human esophageal cancer using Versican Rabbit mAb (NBP3-15841) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Versican-Antibody-6D8K9-Immunohistochemistry-Paraffin-NBP3-15841-img0002.jpg)
![ELISA: Human Versican ELISA Kit (Colorimetric) [NBP2-75354] - Samples were spiked with high concentrations of Human Versican and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75354_human-versican-elisa-kit-colorimetric-132202416221054.jpg "ELISA: Human Versican ELISA Kit (Colorimetric) [NBP2-75354] - Samples were spiked with high concentrations of Human Versican and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Versican ELISA Kit (Colorimetric) [NBP2-75354] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75354_human-versican-elisa-kit-colorimetric-432024915820.jpg)
![ELISA: Human Versican ELISA Kit (Chemiluminescence) [NBP2-75353] - Samples were spiked with high concentrations of Human Versican and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75353_human-versican-elisa-kit-chemiluminescence-132202416235763.jpg "ELISA: Human Versican ELISA Kit (Chemiluminescence) [NBP2-75353] - Samples were spiked with high concentrations of Human Versican and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Versican ELISA Kit (Chemiluminescence) [NBP2-75353] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75353_human-versican-elisa-kit-chemiluminescence-4320248582551.jpg)
