VEGFR1/Flt-1 Antibody - Azide and BSA Free

Western Blot: VEGFR1/Flt-1 Antibody [NBP1-18631] - The polyclonal antibody was raised against the unique C-terminal end of the native human soluble Flt-1 (amino acids: CNKKAVFSRISKFKSTRNDSTTQSNVKH). The Protein-A purified antibody recognizes the recombinant soluble Flt-1 (native) but not the recombinant soluble Flt-1(D1-5) both produced in insect cells. Because of the different glycosylation of insect cells compared to mammalian cells the MW of sFlt-1 (native) is about 96 kDa instead of about 110 kDa of the naturally occurring sFlt-1. The antibody did not cross react with recombinant human soluble KDR (D1-7). The monoclonal anti-human VEGFR-1/Flt-1 antibody raised against the first 5 Ig-like loops of Flt-1 recognizes both forms.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

VEGFR1/Flt-1 Antibody - Azide and BSA Free Summary

• Immunogen: A peptide of the C-terminal end of native human soluble VEGFR-1/Flt-1 (GEHCNKKAVFSRISKFKSTR NDSTTQSNVKH)
• Marker: Endothelial Cell Marker
• Specificity: soluble VEGFR-1/Flt-1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FLT1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-5 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form.

Alternate Names for VEGFR1/Flt-1 Antibody - Azide and BSA Free

• EC 2.7.10
• EC 2.7.10.1
• FLT
• FLT1
• Flt-1
• Fms-like tyrosine kinase 1
• fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor)
• FRT
• Tyrosine-protein kinase FRT
• Tyrosine-protein kinase receptor FLT
• vascular endothelial growth factor receptor 1
• Vascular permeability factor receptor
• VEGF R1
• VEGFR1
• VEGFR-1

Background

VEGF Receptor 1 (also known as FLT) belongs to the src gene family and shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. The protein acts as a receptor for VEGF, VEGFB and PGF. An alternatively spliced form of the gene produces a soluble protein (sFlt1) which binds vascular endothelial growth factor (VEGF) with high affinity. sFlt1 has a higher affinity for VEGF indicating that it may function as an inhibitor in the VEGF response. VEGF Receptor 1 is specifically expressed in most vascular endothelial cells and peripheral blood monocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FLT1
• Entrez:
  • 2321(Human)