VEGFR1/Flt-1 Antibody (MAB0702) - Azide and BSA Free

Western Blot: VEGF R1/Flt-1 Antibody (MAB0702) [NBP2-21963] - HUVECs, preincubated with anti-VEGFR1 at indicated dose for 20mins, were stimulated with human VEGF for 20 mins and then the level of p-VEGFR1 was detected using anti-phospho-VEGFR1 (Y1213).

Product Discontinued

VEGFR1/Flt-1 Antibody (MAB0702) - Azide and BSA Free Summary

• Immunogen: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with purified extracellular domain of recombinant human VEGFR1. VEGFR-1 ( Antagonistic) EC domain, AAC50060.
• Marker: Endothelial Cell Marker
• Specificity: This antibody recognizes both human and mouse VEGFR1 in western blot. It shows no cross-reactivity with human or mouse VEGFR2.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FLT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Western Blot 1:400-1000

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for VEGFR1/Flt-1 Antibody (MAB0702) - Azide and BSA Free

• EC 2.7.10
• EC 2.7.10.1
• FLT
• FLT1
• Flt-1
• Fms-like tyrosine kinase 1
• fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor)
• FRT
• Tyrosine-protein kinase FRT
• Tyrosine-protein kinase receptor FLT
• vascular endothelial growth factor receptor 1
• Vascular permeability factor receptor
• VEGF R1
• VEGFR1
• VEGFR-1

Background

Receptors for VEGF, VEGFB and PGF have tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. VEGF and its high-affinity binding receptors--the tyrosine kinases FLK1 and FLT1--are thought to be important for the development of embryonic vasculature. It has been shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity, playing an inhibitory role in angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FLT1
• Uniprot:
  • AAC50060()