VEGFR1/Flt-1 Antibody (CL0345) - Azide and BSA Free

Analysis in human placenta and tonsil tissues using NBP3-43978 antibody. Corresponding VEGFR1/Flt-1 RNA-seq data are presented for the same tissues.

Lane 1: Marker [kDa]Lane 2: Human cell line RT-4

Staining of human kidney shows strong cytoplasmic positivity in endothelial cells.

Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Staining of human small intestine shows moderate to strong cytoplasmic positivity in endothelial cells, as well as positivity in smooth muscle cells.

Staining of human tonsil shows no positivity in lymphoid cells as expected.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL0345
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

VEGFR1/Flt-1 Antibody (CL0345) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a recombinant protein sequence of P17948, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: FLT1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for VEGFR1/Flt-1 Antibody (CL0345) - Azide and BSA Free

• EC 2.7.10
• EC 2.7.10.1
• FLT
• FLT1
• Flt-1
• Fms-like tyrosine kinase 1
• fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor)
• FRT
• Tyrosine-protein kinase FRT
• Tyrosine-protein kinase receptor FLT
• vascular endothelial growth factor receptor 1
• Vascular permeability factor receptor
• VEGF R1
• VEGFR1
• VEGFR-1

Background

Receptors for VEGF, VEGFB and PGF have tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. VEGF and its high-affinity binding receptors--the tyrosine kinases FLK1 and FLT1--are thought to be important for the development of embryonic vasculature. It has been shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity, playing an inhibitory role in angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FLT1