VEGF Antibody [Unconjugated] - 164

VEGF164was detected in perfusion fixed frozen sections of rat kidney using 15 µg/mL Goat Anti-Rat VEGF164Antigen Affinity-purified Polyclonal Antibody (Catalog # AF564) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Recombinant Rat VEGF164 (Catalog # 564-RV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat VEGF164 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat VEGF 164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF564). The ND50 is typically 0.2-0.6 µg/mL.

Western blot shows 25 ng of Recombinant Rat VEGF 164 (Catalog # 564-RV), Recombinant Human VEGF 165 (Catalog # 293-VE) and Recombinant Mouse VEGF 164 (Catalog # 493-MV). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Rat VEGF 164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF564) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for VEGF at approximately 20-25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.This antibody does not detect natural VEGF in lysates from cell lines or tissues.

Expression of proteins relevant to kidney regeneration in female MWF rats.Immunohistochemical staining of VEGF in female animals receiving saline or MET transplant, after 6 weeks. Scale bars = 50 μm. Representative immunofluorescence staining (red) of FGF2, HGF, IGF-1 and Pax-2 in female rats receiving saline or MET, on renal tissues labeled with WGA-lectin (green) and DAPI (blue). Scale bars = 50 μm, = 25 μm (for FGF2 and IGF-1). Semiquantitative analysis of immunohistochemical stainings of VEGF, FGF2, HGF, IGF-1 and Pax-2 in female MWF rats receiving saline or MET. Data are expressed as mean ± SE (n = 3/group). Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0120235), licensed under a CC-BY license. Not internally tested by R&D Systems.

Depletion of BAMs attenuates ischemia-induced pial and cortical vascular permeability. BAMs were depleted by i.c.v. administration of clodronate liposomes (CL) 4 days prior to ischemia. For treatment control, rats received vehicle liposomes (V). a) Vegfa mRNA was studied in cortical and subcortical regions of the ipsilateral (ipsi, ischemic) and the contralateral (contra) brain hemispheres. Ischemia-induced expression of Vegfa mRNA at 24 h is attenuated in the cortex of the CL rats versus the V rats (n = 6 per group) (Mann Whitney test, p = 0.041). b) Western blotting of cortical and subcortical brain tissue (ipsilateral) 24 h post-ischemia shows the VEGF164 isoform of VEGF-A detected as two bands corresponding to the homodimeric and monomeric forms at approximately 54 kDa and 24 kDa. Quantification of signal intensity of the 54-kDa band shows reduced expression in the cortex of rats receiving CL vs. rats receiving V (n = 6 per group) (Mann-Whitney test, **p = 0.002). c) Ischemia increases the permeability of pial vessels (arrows), as assessed by Evans blue extravasation 24 h post-ischemia, and BAM depletion attenuates the effect. Images of one representative coronal brain section per rat of each treatment group show Evans blue extravasation in the ipsilateral hemisphere. Rats receiving clodronate show negligible Evans blue in the cortex (arrows in the schematic representation at the right hand side). The volume of tissue showing Evans blue extravasation is reduced in the cortex, but not in subcortical zones, of the CL group (n = 8) versus the V group (n = 7) (Mann-Whitney test, *p = 0.014). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30092836), licensed under a CC-BY license. Not internally tested by R&D Systems.

Expression of mRNAs and proteins relevant to kidney regeneration in male MWF rats.(A) Real Time RT-PCR analysis of VEGF, FGF2, HGF, IGF-1 and Pax-2 in whole host renal tissues of male MWF rats receiving saline or metanephroi (MET) (in adjacent area), 6 weeks after transplantation. Data are expressed as mean ± SE; *P < 0.05, **P < 0.01 vs saline (n = 4 rats/group, VEGF n = 6 rats/group). (B) Immunohistochemical staining of VEGF (arrows) in male animals receiving saline or MET transplant, after 6 weeks. Scale bars = 50 μm. Representative immunofluorescence stainings (red) of FGF2, HGF, IGF-1 and Pax-2 in rats receiving saline or MET, on renal tissues labeled with WGA-lectin (green) and DAPI (blue). Scale bars = 50 μm, = 25 μm (for FGF2 and IGF-1). Semiquantitative analysis of immunohistochemical staining of VEGF, FGF2, HGF, IGF-1 and Pax-2 in male MWF rats receiving saline or MET. Data are expressed as mean ± SE; *P < 0.05, **P < 0.01 vs saline (n = 3/group). Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0120235), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effect of fibroblasts transplanted under the kidney capsule of male MWF rats.(A) Histological appearance of renal tissue from male MWF rat transplanted with fibroblasts in adjacent (left, arrow) or distant (right) area. Scale bars = 200 μm. (B) Quantification of endothelium volume density (Vv), peritubular capillary length density (Jv), nitrotyrosine (NT)-positive tubuli, TUNEL-positive cells and Ki-67-positive cells, in renal tissues of MWF rats receiving fibroblasts or saline. Data are expressed as mean ± SE (n = 3 rats/group). (C) Representative immunohistochemical images of SMP30 (green), NCAM (red), VEGF (brown signal), FGF2 (red), HGF (red), IGF-1 (red) and Pax-2 (red) in male rats with fibroblast transplant. Sections are co-stained with rhodamine-LCA (in SMP30 panel, red) or with FITC-WGA lectin (in panels showing NCAM, FGF2, HGF, IGF-1, Pax2, green) and DAPI (blue). Scale bars = 50 μm. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0120235), licensed under a CC-BY license. Not internally tested by R&D Systems.

Expression of mRNAs and proteins relevant to kidney regeneration in male MWF rats.(A) Real Time RT-PCR analysis of VEGF, FGF2, HGF, IGF-1 and Pax-2 in whole host renal tissues of male MWF rats receiving saline or metanephroi (MET) (in adjacent area), 6 weeks after transplantation. Data are expressed as mean ± SE; *P < 0.05, **P < 0.01 vs saline (n = 4 rats/group, VEGF n = 6 rats/group). (B) Immunohistochemical staining of VEGF (arrows) in male animals receiving saline or MET transplant, after 6 weeks. Scale bars = 50 μm. Representative immunofluorescence stainings (red) of FGF2, HGF, IGF-1 and Pax-2 in rats receiving saline or MET, on renal tissues labeled with WGA-lectin (green) and DAPI (blue). Scale bars = 50 μm, = 25 μm (for FGF2 and IGF-1). Semiquantitative analysis of immunohistochemical staining of VEGF, FGF2, HGF, IGF-1 and Pax-2 in male MWF rats receiving saline or MET. Data are expressed as mean ± SE; *P < 0.05, **P < 0.01 vs saline (n = 3/group). Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0120235), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Rt
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated

VEGF Antibody [Unconjugated] - 164 Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat VEGF₁₆₄
  Ala27-Arg190
  Accession # AAL07526
• Specificity: Detects rat VEGF in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) VEGF₁₆₅ and rhVEGF₁₂₁ is observed and less than 2% cross-reactivity with rhVEGF-B, recombinant mouse (rm) VEGF-B, rhVEGF-C, rhVEGF-D, and rmVEGF-D is observed. In Western blots, detection of recombinant mouse VEGF₁₆₅ is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: VEGFA
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.2-0.6 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGF Antibody [Unconjugated] - 164

• MVCD1
• VAS
• vascular endothelial growth factor A
• Vascular permeability factor
• Vasculotropin
• VEGF
• VEGFA
• VEGF-A
• VEGFMGC70609
• VPF
• VPFvascular endothelial growth factor

Background

Vascular Endothelial Growth Factor (VEGF or VEGF-A), also known as Vascular Permeability Factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure. VEGF164 appears to be the most abundant and potent isoform, followed by VEGF120 and VEGF188. Rat VEGF164 is an approximately 25 kDa molecular weight protein sharing 97% aa sequence identity with corresponding regions of mouse, 88% with human and bovine, 90% with feline and equine, and 89% with canine and porcine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. Human VEGF165 binds the Semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2. VEGF is required during embryogenesis and functions to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M (OSM) and TNF-alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VEGF Antibody (AF564)(48)

Publications using AF564

• JR Jerome, D Deliyanti, V Suphapimol, P Kolkhof, JL Wilkinson- Finerenone, a Non-Steroidal Mineralocorticoid Receptor Antagonist, Reduces Vascular Injury and Increases Regulatory T-Cells: Studies in Rodents with Diabetic and Neovascular Retinopathy International Journal of Molecular Sciences, 2023-01-25;24(3):. 2023-01-25 [PMID: 36768656] (IHC, Transgenic Rat)
• Luis F. Martins, Ilaria Brambilla, Alessia Motta, Stefano de Pretis, Ganesh Parameshwar Bhat, Aurora Badaloni et al. Motor neurons use push-pull signals to direct vascular remodeling critical for their connectivity Neuron 12/21/2022 [PMID: 36240771]
• Luis F. Martins, Ilaria Brambilla, Alessia Motta, Stefano de Pretis, Ganesh Parameshwar Bhat, Aurora Badaloni et al. Motor neurons use push-pull signals to direct vascular remodeling critical for their connectivity Neuron 2022-12-21 [PMID: 36240771] (Neutralization, Mouse)
• C Baron-Meng, A Bocquet, A Richard, AL Guihot, B Toutain, P Pacaud, C Fassot, G Loirand, D Henrion, L Loufrani Sildenafil-Induced Revascularization of Rat Hindlimb Involves Arteriogenesis through PI3K/AKT and eNOS Activation International Journal of Molecular Sciences, 2022-05-16;23(10):. 2022-05-16 [PMID: 35628350] (Neutralization, Rat)
• Peter Baluk, Donald M. McDonald Imaging Blood Vessels and Lymphatics in Mouse Trachea Wholemounts Methods in Molecular Biology 9/6/2022 [PMID: 35099733]
• S Bürger, J Meng, A Zwanzig, M Beck, M Pankonin, P Wiedemann, W Eichler, JD Unterlauft Pigment Epithelium-Derived Factor (PEDF) Receptors Are Involved in Survival of Retinal Neurons International Journal of Molecular Sciences, 2020-12-31;22(1):. 2020-12-31 [PMID: 33396450] (Neutralization, Mouse)
• Yujun Luo, Beibei Wang, Jianhua Liu, Faxin Ma, Dongling Luo, Zhongwen Zheng et al. Ginsenoside RG1 enhances the paracrine effects of bone marrow-derived mesenchymal stem cells on radiation induced intestinal injury Aging (Albany NY) 1/15/2021 [PMID: 33293477]
• Johanna H. Meyer, Janine Marx, Claudine Strack, Frank G. Holz, Steffen Schmitz-Valckenberg Imaging of Therapeutic Effects of Anti-Vascular Endothelial Growth Factor Inhibitors by Optical Coherence Tomography Angiography in a Rat Model Translational Vision Science & Technology 6/25/2020 [PMID: 32832234]
• Céline Borras, Kimberley Delaunay, Yousri Slaoui, Toufik Abache, Sylvie Jorieux, Marie-Christine Naud et al. Mechanisms of FH Protection Against Neovascular AMD Frontiers in Immunology 4/3/2020 [PMID: 32318056]
• D Asano, M Hokazono, S Hirano, A Morita, T Nakahara Cellular Mechanisms of Angiogenesis in Neonatal Rat Models of Retinal Neurodegeneration Int J Mol Sci, 2019-09-25;20(19):. 2019-09-25 [PMID: 31557901] (ICC, Rat)
• Park MH, Kim AK, Manandhar S et al. CCN1 interlinks integrin and Hippo pathway to autoregulate tip cell activity Elife 8/20/2019 [PMID: 31429823]
• Richard Filek, Phil Hooper, Tom G Sheidow, Hong Liu, Subrata Chakrabarti, Cindy ML Hutnik <p>Safety of anti-VEGF treatments in a diabetic rat model and retinal cell culture</p> Clinical Ophthalmology 7/1/2019 [PMID: 31308619]
• Rahmeh Othman, Elvire Vaucher, Réjean Couture Bradykinin Type 1 Receptor – Inducible Nitric Oxide Synthase: A New Axis Implicated in Diabetic Retinopathy Frontiers in Pharmacology 3/29/2019 [PMID: 30983997]
• Peter Baluk, Donald M. McDonald Imaging Lymphatics in Mouse Lungs Methods in Molecular Biology 4/29/2019 [PMID: 30242759]
• T Lind, R Lugano, AM Gustafson, M Norgård, A van Haerin, A Dimberg, H Melhus, SP Robertson, G Andersson Bones in human CYP26B1 deficiency and rats with hypervitaminosis A phenocopy Vegfa overexpression Bone Rep, 2018-06-21;9(0):27-36. 2018-06-21 [PMID: 30003121] (IHC, Rat)
• L Melly, G Cerino, A Frobert, S Cook, MN Giraud, T Carrel, HT Tevaearai, F Eckstein, B Rondelet, A Marsano, A Banfi Myocardial infarction stabilization by cell-based expression of controlled Vascular Endothelial Growth Factor levels J. Cell. Mol. Med., 2018-02-25;0(0):. 2018-02-25 [PMID: 29478261] (IHC, Rat)
• S Khalili, Y Shifrin, J Pan, J Belik, K Mireskanda The effect of a single anti-Vascular Endothelial Growth Factor injection on neonatal growth and organ development: In-vivo study Exp. Eye Res., 2018-02-03;169(0):54-59. 2018-02-03 [PMID: 29421328] (In Vivo, Rat)
• Pierre-Raphaël Rothschild, Sawsen Salah, Marianne Berdugo, Emmanuelle Gélizé, Kimberley Delaunay, Marie-Christine Naud et al. ROCK-1 mediates diabetes-induced retinal pigment epithelial and endothelial cell blebbing: Contribution to diabetic retinopathy Scientific Reports 8/18/2017 [PMID: 28821742]
• Pierre-Raphaël Rothschild, Sawsen Salah, Marianne Berdugo, Emmanuelle Gélizé, Kimberley Delaunay, Marie-Christine Naud et al. ROCK-1 mediates diabetes-induced retinal pigment epithelial and endothelial cell blebbing: Contribution to diabetic retinopathy Scientific Reports 2017-08-18 [PMID: 28821742] (Immunohistochemistry, Rat)
• P Mirabelli, A Mukwaya, A Lennikov, M Xeroudaki, B Peebo, M Schaupper, N Lagali Genome-wide expression differences in anti-Vegf and dexamethasone treatment of inflammatory angiogenesis in the rat cornea Sci Rep, 2017-08-15;7(1):7616. 2017-08-15 [PMID: 28811496] (Neutralization, Rat)
• LI Al Asoom Coronary angiogenic effect of long-term administration of Nigella sativa BMC Complement Altern Med, 2017-06-13;17(1):308. 2017-06-13 [PMID: 28610577] (IHC, Rat)
• Roberta Coroniti, Rafal Fario, Didier J. Nuno, Laszlo Otvos, Laura Scolaro, Eva Surmacz Designer Leptin Receptor Antagonist Allo-aca Inhibits VEGF Effects in Ophthalmic Neoangiogenesis Models Frontiers in Molecular Biosciences 10/13/2016 [PMID: 27790618]
• Minoru Sasaki, Takanobu Sakurai, Aiko Ishii, Kenta Matsue, Yutaka Nakanishi, Shunsuke Tsutsumi et al. Evaluation of potential activity of luseogliflozin on vascular proliferation in the mesenteric lymph node with or without vascular tumors in Sprague-Dawley rats in a carcinogenicity study Journal of Toxicologic Pathology 1/18/2016 [PMID: 27182112]
• Deliyanti D, Zhang Y, Khong F, Berka D, Stapleton D, Kelly D, Wilkinson-Berka J FT011, a Novel Cardiorenal Protective Drug, Reduces Inflammation, Gliosis and Vascular Injury in Rats with Diabetic Retinopathy. PLoS ONE, 2015-07-29;10(7):e0134392. 2015-07-29 [PMID: 26222724] (IHC-P, Rat)
• Jonathan P. Wigg, Hong Zhang, Dong Yang A Quantitative and Standardized Method for the Evaluation of Choroidal Neovascularization Using MICRON III Fluorescein Angiograms in Rats PLOS ONE 5/29/2015 [PMID: 26024231]
• Imberti B, Corna D, Rizzo P, Xinaris C, Abbate M, Longaretti L, Cassis P, Benedetti V, Benigni A, Zoja C, Remuzzi G, Morigi M Renal primordia activate kidney regenerative events in a rat model of progressive renal disease. PLoS ONE, 2015-03-26;10(3):e0120235. 2015-03-26 [PMID: 25811887] (IHC, Rat)
• Wang H, Yang Z, Jiang Y, Flannery J, Hammond S, Kafri T, Vemuri S, Jones B, Hartnett M Quantitative analyses of retinal vascular area and density after different methods to reduce VEGF in a rat model of retinopathy of prematurity. Invest Ophthalmol Vis Sci, 2014-02-04;55(2):737-44. 2014-02-04 [PMID: 24425858] (Neutralization, Rat)
• Daniel C, Vogelbacher R, Stief A, Grigo C, Hugo C Long-term gene therapy with thrombospondin 2 inhibits TGF-beta activation, inflammation and angiogenesis in chronic allograft nephropathy. PLoS ONE, 2013-12-23;8(12):e83846. 2013-12-23 [PMID: 24376766] (IHC, Rat)
• Dharmani P, De Simone C, Chadee K The probiotic mixture VSL#3 accelerates gastric ulcer healing by stimulating vascular endothelial growth factor. PLoS ONE, 2013-03-06;8(3):e58671. 2013-03-06 [PMID: 23484048] (Neutralization, Rat)
• McCloskey M, Wang H, Jiang Y, Smith G, Strange J, Hartnett M Anti-VEGF antibody leads to later atypical intravitreous neovascularization and activation of angiogenic pathways in a rat model of retinopathy of prematurity. Invest Ophthalmol Vis Sci, 2013-03-21;54(3):2020-6. 2013-03-21 [PMID: 23449716] (Neutralization, Rat)
• Scott T. Hollenbeck, Annika Senghaas, Issei Komatsu, Ying Zhang, Detlev Erdmann, Bruce Klitzman Tissue engraftment of hypoxic-preconditioned adipose-derived stem cells improves flap viability Wound Repair and Regeneration 11/1/2012 [PMID: 23110692]
• Scott T. Hollenbeck, Annika Senghaas, Issei Komatsu, Ying Zhang, Detlev Erdmann, Bruce Klitzman Tissue engraftment of hypoxic-preconditioned adipose-derived stem cells improves flap viability Wound Repair and Regeneration 2012-11-01 [PMID: 23110692] (Neutralization, Rat)
• Rohin K. Iyer, Devang Odedra, Loraine L.Y. Chiu, Gordana Vunjak-Novakovic, Milica Radisic Vascular Endothelial Growth Factor Secretion by Nonmyocytes Modulates Connexin-43 Levels in Cardiac Organoids Tissue Engineering Part A 9/1/2012 [PMID: 22519405]
• Benjamin A. Hoff, Mahaveer S. Bhojani, John Rudge, Thomas L. Chenevert, Charles R. Meyer, Stefanie Galbán et al. DCE and DW-MRI monitoring of vascular disruption following VEGF-Trap treatment of a rat glioma model NMR in Biomedicine 7/1/2012 [PMID: 22190279]
• Benjamin A. Hoff, Mahaveer S. Bhojani, John Rudge, Thomas L. Chenevert, Charles R. Meyer, Stefanie Galbán et al. DCE and DW-MRI monitoring of vascular disruption following VEGF-Trap treatment of a rat glioma model NMR in Biomedicine 2012-07-01 [PMID: 22190279] (ELISA Capture, Rat)
• G M Palmer, Z Tiran, Z Zhou, M E Capozzi, W Park, C Coletta et al. A novel angiopoietin-derived peptide displays anti-angiogenic activity and inhibits tumour-induced and retinal neovascularization British Journal of Pharmacology 3/1/2012 [PMID: 21943108]
• Olivera Nesic, Laura M. Sundberg, Juan J. Herrera, Venkata U.L. Mokkapati, Julieann Lee, Ponnada A. Narayana Vascular Endothelial Growth Factor and Spinal Cord Injury Pain Journal of Neurotrauma 10/1/2010 [PMID: 20698758]
• Westenbrink BD, Ruifrok WP, Voors AA Vascular endothelial growth factor is crucial for erythropoietin-induced improvement of cardiac function in heart failure. Cardiovasc. Res., 2010-02-05;87(1):30-9. 2010-02-05 [PMID: 20139114] (Neutralization, Rat)
• Ana G. Barbeito, Laura Martinez-Palma, Marcelo R. Vargas, Mariana Pehar, Nelly Mañay, Joseph S. Beckman et al. Lead exposure stimulates VEGF expression in the spinal cord and extends survival in a mouse model of ALS Neurobiology of Disease 3/1/2010 [PMID: 19914377]
• Irani Y, Brereton HM, Tilton RG Production of scFv antibody fragments from a hybridoma with functional activity against human vascular endothelial growth factor. Hybridoma (Larchmt), 2009-06-01;28(3):205-9. 2009-06-01 [PMID: 19445630] (Binding Assay, Human, Mouse, Rat)
• Lin HH, Chen YH, Chang PF, Lee YT, Yet SF, Chau LY Heme oxygenase-1 promotes neovascularization in ischemic heart by coinduction of VEGF and SDF-1. J. Mol. Cell. Cardiol., 2008-05-02;45(1):44-55. 2008-05-02 [PMID: 18534615] (Neutralization, Mouse)
• Marko SB, Damon DH VEGF promotes vascular sympathetic innervation. Am. J. Physiol. Heart Circ. Physiol., 2008-04-11;294(6):H2646-52. 2008-04-11 [PMID: 18408130] (ICC, IHC-Fr, Neutralization, Rat)
• Hartnett ME, Martiniuk D, Byfield G, Geisen P, Zeng G, Bautch VL Neutralizing VEGF decreases tortuosity and alters endothelial cell division orientation in arterioles and veins in a rat model of ROP: relevance to plus disease. Invest. Ophthalmol. Vis. Sci., 2008-03-31;49(7):3107-14. 2008-03-31 [PMID: 18378573] (Neutralization, Rat)
• Yeh WL, Lin CJ, Fu WM Enhancement of glucose transporter expression of brain endothelial cells by vascular endothelial growth factor derived from glioma exposed to hypoxia. Mol. Pharmacol., 2007-10-17;73(1):170-7. 2007-10-17 [PMID: 17942749] (Neutralization, Rat)
• Advani A, Kelly DJ, Advani SL, Cox AJ, Thai K, Zhang Y, White KE, Gow RM, Marshall SM, Steer BM, Marsden PA, Rakoczy PE, Gilbert RE Role of VEGF in maintaining renal structure and function under normotensive and hypertensive conditions. Proc. Natl. Acad. Sci. U.S.A., 2007-08-28;104(36):14448-53. 2007-08-28 [PMID: 17726104] (Neutralization, Rat)
• Baron-Menguy C, Bocquet A, Guihot AL, Chappard D, Amiot MJ, Andriantsitohaina R, Loufrani L, Henrion D Effects of red wine polyphenols on postischemic neovascularization model in rats: low doses are proangiogenic, high doses anti-angiogenic. FASEB J., 2007-06-26;21(13):3511-21. 2007-06-26 [PMID: 17595348] (Neutralization, Rat)
• Yeh WL, Lu DY, Lin CJ, Liou HC, Fu WM Inhibition of hypoxia-induced increase of blood-brain barrier permeability by YC-1 through the antagonism of HIF-1alpha accumulation and VEGF expression. Mol. Pharmacol., 2007-05-18;72(2):440-9. 2007-05-18 [PMID: 17513385] (Neutralization, Rat)
• Shimojo N, Jesmin S, Zaedi S, Otsuki T, Maeda S, Yamaguchi N, Aonuma K, Hattori Y, Miyauchi T Contributory role of VEGF overexpression in endothelin-1-induced cardiomyocyte hypertrophy. Am. J. Physiol. Heart Circ. Physiol., 2007-03-16;293(1):H474-81. 2007-03-16 [PMID: 17369464] (Neutralization, Rat)

FAQs for VEGF Antibody (AF564). (Showing 1 - 1 of 1 FAQs).

1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
   • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

Bioinformatics

• Gene Symbol: VEGFA
• Uniprot:
  • AAL07526()