VEGF Antibody [Unconjugated] - 164


Western blot shows lysates of human lung tissue, human kidney tissue, and human liver tissue. PVDF membrane was probed with 5-10 µg/mL of Goat Anti-Human VEGF165 Antigen Affinity-purified Polyclonal Antibody (Catalog # more
VEGF was detected in immersion fixed human umbilical vein endothelial cells (HUVECs) using Goat Anti-Human VEGF165 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-293-NA) at 10 µg/mL for 3 hours at room more
VEGF was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Goat Anti-Human VEGF 165 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-293-NA) at more
Recombinant Human VEGF165 (Catalog # 293-VE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human VEGF165 (10 more

Product Details

Reactivity HuSpecies Glossary
Applications WB, IHC, B/N, ICC/IF

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VEGF Antibody [Unconjugated] - 164 Summary

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF165
Accession # NP_001165097.1
Detects human VEGF in direct ELISAs and Western blots.
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Neutralization 0.02-0.12 ug/mL
  • Western Blot 5-10 ug/mL
Reviewed Applications
Read 2 Reviews rated 4.5
AF-293-NA in the following applications:

Read Publications using
AF-293-NA in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGF Antibody [Unconjugated] - 164

  • MVCD1
  • VAS
  • vascular endothelial growth factor A
  • Vascular permeability factor
  • Vasculotropin
  • VEGF
  • VEGF-A
  • VEGFMGC70609
  • VPF
  • VPFvascular endothelial growth factor


Vascular Endothelial Growth Factor (VEGF or VEGF-A), also known as Vascular Permeability Factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. Human VEGF165 is an approximately 44 kDa molecular weight homodimer sharing 88% aa sequence identity with corresponding regions of mouse and rat, 96% with porcine, 95% with canine, and 93% with feline, equine and bovine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. VEGF165 binds the Semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2. VEGF is required during embryogenesis and functions to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M (OSM) and TNF-alpha.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VEGF Antibody (AF-293-NA)(50)

We have publications tested in 10 confirmed species: Human, Mouse, Avian - Quail, Bovine, Chicken, Guinea Pig, Porcine, Primate - Macaca mulatta (Rhesus Macaque), Rabbit, Transgenic Rabbit.

We have publications tested in 14 applications: Affinity Assay, Bioassay, Column, ELISA, ELISA Development, ELISA Development (Capture), ELISA Development (Detection), ICC, IHC, IHC-P, Immunoassay Development, Immunoprecipitation, Neutralization, Western Blot.

Filter By Application
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ELISA Development
ELISA Development (Capture)
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Avian - Quail
Guinea Pig
Primate - Macaca mulatta (Rhesus Macaque)
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Showing Publications 1 - 10 of 50. Show All 50 Publications.
Publications using AF-293-NA Applications Species
WJ Brewer, AM Xet-Mull, A Yu, MI Sweeney, EM Walton, DM Tobin Macrophage NFATC2 mediates angiogenic signaling during mycobacterial infection Cell Reports, 2022;41(11):111817. 2022 [PMID: 36516756] (ICC, Human) ICC Human
GL Lehmann, M Ginsberg, DJ Nolan, C Rodríguez, J Martínez-G, S Zeng, AP Voigt, RF Mullins, S Rafii, E Rodriguez-, I Benedicto Retinal Pigment Epithelium-Secreted VEGF-A Induces Alpha-2-Macroglobulin Expression in Endothelial Cells Cells, 2022;11(19):. 2022 [PMID: 36230937] (Neutralization, Human) Neutralization Human
H Han, Y Kim, H Mo, SH Choi, K Lee, YA Rim, JH Ju Preferential stimulation of melanocytes by M2 macrophages to produce melanin through vascular endothelial growth factor Scientific Reports, 2022;12(1):6416. 2022 [PMID: 35440608] (Bioassay, Human) Bioassay Human
P Li, H Xin, L Lu Extracellular vesicle-encapsulated microRNA-424 exerts inhibitory function in ovarian cancer by targeting MYB Journal of Translational Medicine, 2021;19(1):4. 2021 [PMID: 33407591] (Western Blot, Human) Western Blot Human
JM Murrieta-C, RN Gutiérrez-, HM El-Shorafa, T Groten, S Rodríguez-, ME Cancino-Di, JC Cancino-Di, RR Favaro, UR Markert, DM Morales-Pr Role of IL-36 Cytokines in the Regulation of Angiogenesis Potential of Trophoblast Cells International Journal of Molecular Sciences, 2020;22(1):. 2020 [PMID: 33396613] (Western Blot, Human) Western Blot Human
D Dong, Y Fu, F Chen, J Zhang, H Jia, J Li, H Wang, J Wen Hyperoxia sensitizes hypoxic HeLa cells to ionizing radiation by downregulating HIF?1&amp;alpha and VEGF expression Mol Med Rep, 2021;23(1):. 2021 [PMID: 33215223] (Western Blot, Human) Western Blot Human
ML Carbone, PM Lacal, S Messinese, L De Giglio, C Pozzilli, S Persechino, C Mazzanti, CM Failla, G Pagnanelli Multiple Sclerosis Treatment and Melanoma Development Int J Mol Sci, 2020;21(8):. 2020 [PMID: 32331328] (ELISA, Human) ELISA Human
S Donzelli, A Sacconi, C Turco, E Gallo, E Milano, I Iosue, G Blandino, F Fazi, G Fontemaggi Paracrine Signaling from Breast Cancer Cells Causes Activation of ID4 Expression in Tumor-Associated Macrophages Cells, 2020;9(2):. 2020 [PMID: 32054109] (Western Blot, Human) Western Blot Human
JH Wu, YN Li, AQ Chen, CD Hong, CL Zhang, HL Wang, YF Zhou, PC Li, Y Wang, L Mao, YP Xia, QW He, HJ Jin, ZY Yue, B Hu Inhibition of Sema4D/PlexinB1 signaling alleviates vascular dysfunction in diabetic retinopathy EMBO Mol Med, 2020;12(2):e10154. 2020 [PMID: 31943789] (Neutralization, Mouse) Neutralization Mouse
F Njau, N Shushakova, H Schenk, VC Wulfmeyer, R Bollin, J Menne, H Haller Calcium dobesilate reduces VEGF signaling by interfering with heparan sulfate binding site and protects from vascular complications in diabetic mice PLoS ONE, 2020;15(1):e0218494. 2020 [PMID: 31935212] (Neutralization, Human) Neutralization Human
Show All 50 Publications.

Reviews for VEGF Antibody (AF-293-NA) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using AF-293-NA:
Filter by Applications
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Images Ratings Applications Species Date Details
Western Blot VEGF AF-293-NA
reviewed by:
Kyoungrae Kim
WB Human 02/25/2020


ApplicationWestern Blot
Sample TestedSkeletal muscle
reviewed by:
Anonymous -
WB Human 01/07/2015


ApplicationWestern Blot
Sample TestedSee PMID 22897854

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FAQs for VEGF Antibody (AF-293-NA). (Showing 1 - 1 of 1 FAQs).

  1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
    • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

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Bioinformatics Tool for VEGF Antibody (AF-293-NA)

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Blogs on VEGF. Showing 1-10 of 22 blog posts - Show all blog posts.

Understanding ‘Y’ in Breast Cancer: Crucial Role of DNA/RNA-binding Protein YB-1 in the Development, Pre-Invasive, and Metastatic Phases
Jamshed Arslan, Pharm D, PhD In the United States, 1 in 8 women will be diagnosed with breast cancer in her lifetime.1 Despite the prevalence, cancer genesis is a mystery. The heterogeneity of cancers makes it diff...  Read full blog post.

Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysis
By Rosa Moreno, PhD. Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysisPhysiological low levels of oxygen induce normal hypoxic events across biological systems. This hypoxic state activ...  Read full blog post.

Neurovascular signaling for repair enhances brain metastasis
By Jamshed Arslan, Pharm. D., PhD. Stroke    is a leading cause of mortality and morbidity worldwide. Cellular players – neurons, astrocytes, endothelial and stromal cells – involved in post-stroke repair t...  Read full blog post.

mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein ...  Read full blog post.

Chemotherapy-induced metastasis: An unexpected foe?
By Yoskaly Lazo-Fernandez, PhD IntroductionEvidence has accumulated recently indicating that common cancer therapies might stimulate metastasis in a significant number of cancer patients1. In fact, neoadjuvant che...  Read full blog post.

Getting Physical: Link between Lipid Metabolism and Hypoxia Target Genes
By Jamshed Arslan Pharm.D. von Hippel-Lindau (VHL) disease is associated with tumors arising in multiple organs. Activation of hypoxia-inducible factor (HIF)-alpha underlies the VHL disease pathogenesis. In normoxia...  Read full blog post.

The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein ...  Read full blog post.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

The dynamic use of a PCNA antibody in fish, porcine and primate species
Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair.  Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl...  Read full blog post.

The role of HIF-1 Alpha signaling in the retina under hypoxic conditions
Hypoxia inducible factor 1 (HIF-1) is a protein that plays an essential role in hypoxia, or low levels of cellular oxygen. HIF-1 is a heterodimeric protein that consists of a constitutively expressed beta subunit and oxygen related alpha subunit.  ...  Read full blog post.

Showing 1-10 of 22 blog posts - Show all blog posts.
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Recent Reviews


Kyoungrae Kim
Application: WB
Species: Human

Anonymous -
Application: WB
Species: Human


Gene Symbol VEGFA