VE-Cadherin Antibody [Unconjugated]

Images

 
Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human VE‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF938) ...read more
VE‑Cadherin was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human VE‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF938) at 10 µg/mL for 3 hours at ...read more
VE‑Cadherin was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human VE‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF938) at 10 µg/mL for 3 hours at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

VE-Cadherin Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human VE-Cadherin
Asp48-Gln593
Accession # P33151
Specificity
Detects human VE-Cadherin in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse VE‑Cadherin is observed and less than 5% cross-reactivity with recombinant human E-Cadherin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CDH5
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publications using
AF938 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VE-Cadherin Antibody [Unconjugated]

  • 7B4 antigen
  • 7B4
  • cadherin 5, type 2 (vascular endothelium)
  • cadherin 5, type 2, VE-cadherin (vascular epithelium)
  • Cadherin-5
  • CD144 antigen
  • CD144
  • CDH5
  • endothelial-specific cadherin
  • FLJ17376
  • Vascular endothelial cadherin
  • VECadherin
  • VE-Cadherin

Background

Vascular endothelial (VE) - cadherin (VE-CAD), also called 7B4 and cadherin-5, is a member of the cadherin family of cell adhesion molecules. Cadherins are
calcium‑dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. VE-Cadherin has been shown to play important roles in vasculogenesis and angiogenesis. VE-cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human VE-cadherin is a 784 amino acid (aa) residue protein with a 25 aa signal sequence and a 759 aa propeptide. The mature protein begins at amino acid 48 and has a 546 aa extracellular domain, a 27 aa transmembrane domain, and a 164 aa cytoplasmic domain. The human and mouse mature VE-CAD proteins share approximately 74% homology.

  1. Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.
  2. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  3. Overduin, M. et al. (1995) Science 267:386.
  4. Takeichi, M. (1991) Science 251:1451.
  5. Nose, A. et al. (1987) EMBO J. 6:3655.
  6. Carmeliet, P. et al. (1999) Cell 98:147.
  7. Gory-Faure, S. et al. (1999) Development 126:2093.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VE-Cadherin Antibody (AF938)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Flow Cytometry, ICC, IHC, IHC-Fr, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(5)
IHC
(2)
IHC-Fr
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(8)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 10.
Publications using AF938 Applications Species
P Mora, PL Hollier, S Guimbal, A Abelanet, A Diop, L Cornuault, T Couffinhal, S Horng, AP Gadeau, MA Renault, C Chapouly Genetic disruption of the Blood Brain Barrier leads to protective barrier formation at the Glia Limitans PLoS Biol, 2020;18(11):e3000946. 2020 [PMID: 33253145] (IHC, Human) IHC Human
J Ohmes, Y Xiao, F Wang, MD Mikkelsen, TT Nguyen, H Schmidt, A Seekamp, AS Meyer, S Fuchs Effect of Enzymatically Extracted Fucoidans on Angiogenesis and Osteogenesis in Primary Cell Culture Systems Mimicking Bone Tissue Environment Mar Drugs, 2020;18(9):. 2020 [PMID: 32967359] (ICC, Human) ICC Human
F Roudnicky, BK Kim, Y Lan, R Schmucki, V Küppers, K Christense, M Graf, C Patsch, M Burcin, CA Meyer, PD Westenskow, CA Cowan Identification of a combination of transcription factors that synergistically increases endothelial cell barrier resistance Sci Rep, 2020;10(1):3886. 2020 [PMID: 32127614] (ICC, Human) ICC Human
M Riddell, A Nakayama, T Hikita, F Mirzapours, T Kawamura, A Pasha, M Li, M Masuzawa, M Looso, T Steinbache, K Ebnet, M Potente, T Hirose, S Ohno, I Fleming, S Gattenlöhn, PP Aung, T Phung, O Yamasaki, T Yanagi, H Umemura, M Nakayama aPKC controls endothelial growth by modulating c-Myc via FoxO1 DNA-binding ability Nat Commun, 2018;9(1):5357. 2018 [PMID: 30559384] (IHC, Human) IHC Human
K Nonomura, V Lukacs, DT Sweet, LM Goddard, A Kanie, T Whitwam, SS Ranade, T Fujimori, ML Kahn, A Patapoutia Mechanically activated ion channel PIEZO1 is required for lymphatic valve formation Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):. 2018 [PMID: 30482854] (IHC-Fr, Mouse) IHC-Fr Mouse
J Xu, J Liang, YM Meng, J Yan, XJ Yu, CQ Liu, L Xu, SM Zhuang, L Zheng Vascular CXCR4 expression promotes vessel sprouting and sensitivity to sorafenib treatment in hepatocellular carcinoma Clin. Cancer Res, 2017;0(0):. 2017 [PMID: 28223275] (ICC, Human) ICC Human
DeepCAGE transcriptomics identify HOXD10 as transcription factor regulating lymphatic endothelial responses to VEGF-C J Cell Sci, 2016;0(0):. 2016 [PMID: 27199372] (Western Blot, Human) Western Blot Human
Wang J, Tao J, Chen D, Cai J, Irani K, Wang Q, Yuan H, Chen A MicroRNA miR-27b rescues bone marrow-derived angiogenic cell function and accelerates wound healing in type 2 diabetes mellitus. Arterioscler Thromb Vasc Biol, 2014;34(0):99. 2014 [PMID: 24177325] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Niwa A, Heike T, Umeda K A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors. PLoS ONE, 2011;6(7):e22261. 2011 [PMID: 21818303] (ICC, Human) ICC Human
Kelly MA, Hirschi KK Signaling hierarchy regulating human endothelial cell development. Arterioscler. Thromb. Vasc. Biol., 2009;29(5):718-24. 2009 [PMID: 19213939] (ICC, Human) ICC Human

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Secondary Antibodies

 

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Biotin BAF938

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Bioinformatics

Gene Symbol CDH5
Uniprot