VE-Cadherin Antibody (3D5C7) - BSA Free

Western Blot: VE-Cadherin Antibody (3D5C7) [NBP2-37420] - Western blot analysis using CDH5 mouse mAb against MCF-7 (1), A549 (2), HUVE-12 (3) cell lysate, and rat lung (4) tissue lysate.

Flow Cytometry: VE-Cadherin Antibody (3D5C7) [NBP2-37420] - Flow cytometric analysis of Jurkat cells using CDH5 mouse mAb (green) and negative control (purple).

Western Blot: VE-Cadherin Antibody (3D5C7) [NBP2-37420] - Western blot analysis using CDH5 mAb against human CDH5 recombinant protein. (Expected MW is 47.6 kDa)

ELISA: VE-Cadherin Antibody (3D5C7) [NBP2-37420] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu, Rt
• Applications: WB, ELISA, Flow, CyTOF-ready
• Clone: 3D5C7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

VE-Cadherin Antibody (3D5C7) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human VE-Cadherin (AA: 29-223) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDH5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Western Blot 1:500-1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 87.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for VE-Cadherin Antibody (3D5C7) - BSA Free

• 7B4 antigen
• 7B4
• cadherin 5, type 2 (vascular endothelium)
• cadherin 5, type 2, VE-cadherin (vascular epithelium)
• Cadherin-5
• CD144 antigen
• CD144
• CDH5
• endothelial-specific cadherin
• FLJ17376
• Vascular endothelial cadherin
• VECadherin
• VE-Cadherin

Background

VE-cadherin (cadherin-5) is an endothelial specific adhesion molecule that is essential for the maintenance of endothelial barrier function and angiogenesis. VE-cadherin is linked to the actin cytoskeleton through a number of adaptor proteins including a-, â-, and ã-catenin. Cytoskeletal dynamics and phosphorylation regulate VE-cadherin mediated cell-cell adhesion. The cytosolic C-terminal Src kinase (Csk) binds via its SH2 domain to the phosphorylated tyrosine 658 site of VE-cadherin. This interaction regulates density dependent cell proliferation. Phosphorylation of tyrosine 658 has also been shown to lead to the uncoupling of p120-catenin from the cytoplasmic tail of VE-cadherin. This phosphorylation event is sufficient to maintain cells in a mesenchymal state during the cell invasion phase of angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for VE-Cadherin Antibody (NBP2-37420) (1)

Average Rating: 1

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Parisa Shabani IHC-Fr Rat 06/27/2022

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Adult heart
• Species: Rat
• Lot: 191024

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

Bioinformatics

• Gene Symbol: CDH5