VE-Cadherin Antibody (123433)

• Reactivity: Hu
• Applications: WB
• Clone: 123433
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

VE-Cadherin Antibody (123433) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human VE-Cadherin
  Asp48-Gln593
  Accession # P33151
• Specificity: Detects human VE-Cadherin in direct ELISAs and Western blots. Does not cross‑react with recombinant human (rh) Cadherin-8, rhCadherin-17, rhE-Cadherin, rhP-Cadherin, or rmVE-Cadherin.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDH5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VE-Cadherin Antibody (123433)

• 7B4 antigen
• 7B4
• cadherin 5, type 2 (vascular endothelium)
• cadherin 5, type 2, VE-cadherin (vascular epithelium)
• Cadherin-5
• CD144 antigen
• CD144
• CDH5
• endothelial-specific cadherin
• FLJ17376
• Vascular endothelial cadherin
• VECadherin
• VE-Cadherin

Background

Vascular endothelial (VE) - cadherin (VE-CAD), also called 7B4 and cadherin-5, is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha , beta , and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. VE-Cadherin has been shown to play important roles in vasculogenesis and angiogenesis. VE-cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human VE-cadherin is a 784 amino acid (aa) residue protein with a 25 aa signal sequence and a 759 aa propeptide. The mature protein begins at amino acid 48 and has a 546 aa extracellular domain, a 27 aa transmembrane domain, and a 164 aa cytoplasmic domain. The human and mouse mature VE-CAD proteins share approximately 74% homology.

1. Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.
2. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
3. Overduin, M. et al. (1995) Science 267:386.
4. Takeichi, M. (1991) Science 251:1451.
5. Nose, A. et al. (1987) EMBO J. 6:3655.
6. Carmeliet, P. et al. (1999) Cell 98:147.
7. Gory-Faure, S. et al. (1999) Development 126:2093.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VE-Cadherin Antibody (MAB938)(2)

Publications using MAB938

• Farzaneh Fathi, Roghayeh Jalili, Mohammad Amjadi, Mohammad-Reza Rashidi SPR signals enhancement by gold nanorods for cell surface marker detection BioImpacts 10/20/2018 [PMID: 31334038]
• Virrey JJ, Guan S, Li W, Schonthal AH, Chen TC, Hofman FM Increased survivin expression confers chemoresistance to tumor-associated endothelial cells. Am. J. Pathol., 2008-07-03;173(0):575-85. 2008-07-03 [PMID: 18599610] (ICC, Human)

Bioinformatics

• Gene Symbol: CDH5
• Uniprot:
  • P33151()