VDAC2/3 Antibody

Western Blot: VDAC2/3 Antibody [NB100-56463] - analysis of VDAC2/3 in human brain cell lysate in the A) absence and B) presence of immunizing peptide using this antibody. The approximately 55 kD band is not blocked by the immunizing peptide and is believed to result from non-specific cross-reaction.

• Reactivity: Hu, Bv, Ca, Fe, Pm, Mu, Rt, Ch, Ze
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

VDAC2/3 Antibody Summary

• Immunogen: Amino acids 120-132 (KKSGKIKSSYKRE) of human VDAC2 protein were used as the immunogen.
• Predicted Species: Mouse (92%), Rat (92%), Chicken (92%), Zebrafish (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VDAC2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-3 ug/ml
• Control:

  VDAC2 Overexpression Lysate
  Human Brain Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Chimpanzee. 92% sequence identity with Mouse, Rat, Chicken and Zebrafish proteins.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for VDAC2/3 Antibody

• FLJ23841
• hVDAC2
• Outer mitochondrial membrane protein porin 2
• POR
• VDAC-2
• voltage-dependent anion channel 2
• voltage-dependent anion-selective channel protein 2

Background

Voltage-dependent anion channels (VDAC) are small pore-forming membrane proteins that regulate metabolite exchange across the mitochondrial outer membrane. Apart from metabolite transfer, VDAC role has also been suggested during apoptosis. VDAC2 is one of the isoforms of VDAC. It interacts with BAK and functions as a specific inhibitor of BAK-dependent mitochondrial apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VDAC2/3 Antibody (NB100-56463)(2)

Publications using NB100-56463

• Bielaszewska M, Ruter C, Kunsmann L et al. Enterohemorrhagic Escherichia coli Hemolysin Employs Outer Membrane Vesicles to Target Mitochondria and Cause Endothelial and Epithelial Apoptosis. PLoS Pathog 2013-12-01 [PMID: 24348251] (WB, Human)
• Morbt Nora, Mogel Iljana, Kalkhof Stefan et al. Proteome changes in human bronchoalveolar cells following styrene exposure indicate involvement of oxidative stress in the molecular-response mechanism. Proteomics. 2009-11-01 [PMID: 19862763] (WB, Human)

Bioinformatics

• Gene Symbol: VDAC2
• Entrez:
  • 7417(Human)
  • 22334(Mouse)
  • 83531(Rat)
• Uniprot:
  • P45880(Human)