VDAC1 Antibody

Images

 
Western Blot: VDAC1 Antibody [NB100-695] - Lane 1: rat heart whole cell lysate. Lane 2: none. Load: 35 ug per lane. Primary antibody: VDAC/Porin antibody at 1:1,200 for overnight at 4C. Secondary antibody: IRDye800 (TM) ...read more

Product Details

Summary
Reactivity Hu, Av, Bv, Fi, Ma, ReSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

VDAC1 Antibody Summary

Immunogen
VDAC/Porin Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 185-197 of Human VDAC1.
Localization
Mitochondria, cell membrane
Marker
Mitochondria Marker
Specificity
This reacts with all forms of VDAC, including VDAC1, VDAC2 (4 isoforms) and VDAC3 (2 isoforms).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VDAC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:15000-1:30000
  • Immunohistochemistry 1:10-1:500
Application Notes
This affinity purified antibody has been tested for use in ELISA and western blot. Expect a band at ~30-33 kDa in size corresponding - VDAC/Porin by western blotting in the appropriate cell lysate or extract.
Theoretical MW
30.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-695 in the following applications:

  • WB
    4 publications

Reactivity Notes

A BLAST analysis was used to suggest that this antibody would react with VDAC1 from avian, mammalian, aquatic and reptilian sources based on 100% homology for the immunogen sequence.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for VDAC1 Antibody

  • hVDAC1
  • MGC111064
  • Outer mitochondrial membrane protein porin 1
  • Plasmalemmal porin
  • Porin 31HL
  • Porin 31HM
  • PORIN
  • PORIN-31-HL
  • VDAC
  • VDAC-1
  • voltage-dependent anion channel 1
  • voltage-dependent anion-selective channel protein 1

Background

VDAC1 is an outer membrane mitochondrial protein. The VDAC proteins are thought to form aqueous channels, or pores, through which adenine nucleotides cross the outer mitochondrial membrane. VDACs have been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT), and cyclophilin D (CypD), is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis. VDACs are highly expressed in heart, liver and skeletal muscle, where concentrations of mitochondria are at their highest.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VDAC1 Antibody (NB100-695)(4)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Fish.

We have publications tested in 1 application: WB.


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WB
(4)
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(1)
Mouse
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Rat
(1)
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Bioinformatics

Gene Symbol VDAC1
Entrez
Uniprot