VDAC1 Antibody

Images

 
Western Blot: VDAC1 Antibody [NB100-695] - The BNIP3 C-terminus can be phosphorylated. Subcellular localization of BNIP3 phosphomutants, showing Western blot of cytosolic and mitochondrial fractions. Image collected and ...read more
Western Blot: VDAC1 Antibody [NB100-695] - Lane 1: rat heart whole cell lysate. Lane 2: none. Load: 35 ug per lane. Primary antibody: VDAC/Porin antibody at 1:1,200 for overnight at 4C. Secondary antibody: IRDye800 (TM) ...read more
Western Blot: VDAC1 Antibody [NB100-695] - The BNIP3 C-terminus can be phosphorylated. Alkaline extraction of mitochondria-associated proteins, showing alkaline extract of the mitochondrial pellet and the ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, FiSpecies Glossary
Applications WB, ELISA, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

VDAC1 Antibody Summary

Immunogen
VDAC/Porin Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 185-197 of Human VDAC1.
Localization
Mitochondria, cell membrane
Marker
Mitochondria Marker
Specificity
This reacts with all forms of VDAC, including VDAC1, VDAC2 (4 isoforms) and VDAC3 (2 isoforms).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VDAC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:15000-1:30000
  • Immunohistochemistry 1:10-1:500
  • Knockdown Validated
Application Notes
This affinity purified antibody has been tested for use in ELISA and western blot. Expect a band at ~30-33 kDa in size corresponding - VDAC/Porin by western blotting in the appropriate cell lysate or extract.
Theoretical MW
30.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-695 in the following applications:

  • KD
    1 publication
  • WB
    5 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22728245). Rat reactivity reported in scientific literature (DOI: 10.4172/2157-7439.1000220).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VDAC1 Antibody

  • hVDAC1
  • MGC111064
  • Outer mitochondrial membrane protein porin 1
  • Plasmalemmal porin
  • Porin 31HL
  • Porin 31HM
  • PORIN
  • PORIN-31-HL
  • VDAC
  • VDAC-1
  • voltage-dependent anion channel 1
  • voltage-dependent anion-selective channel protein 1

Background

VDAC1 is an outer membrane mitochondrial protein. The VDAC proteins are thought to form aqueous channels, or pores, through which adenine nucleotides cross the outer mitochondrial membrane. VDACs have been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT), and cyclophilin D (CypD), is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis. VDACs are highly expressed in heart, liver and skeletal muscle, where concentrations of mitochondria are at their highest.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-695
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Applications: WB, ELISA, IHC, KD

Publications for VDAC1 Antibody (NB100-695)(5)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Fish.

We have publications tested in 2 applications: KD, WB.


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KD
(1)
WB
(5)
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Human
(2)
Mouse
(1)
Rat
(1)
Fish
(1)
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Showing Publications 1 - 5 of 5.
Publications using NB100-695 Applications Species
Su YC, Chiu HW, Hung JC, Hong JR. Beta-nodavirus B2 protein induces hydrogen peroxide production, leading to Drp1-recruited mitochondrial fragmentation and cell death via mitochondrial targeting. Apoptosis 2014 Jul 10 [PMID: 25008790] (WB, Fish)

Details:
VDAC1 antibody used in WB application at 1:500 dilution as mitochondrial marker for fractions isolated from Grouper/Epinephelus coioides cell line GF-1 (a cell line susceptible to grouper nervous necrosis virus - GNNV) transfected with Flag, betanodavirus B2 gene, and B2-delta as well as MCF-7 cells (Fig 1C). Antibody also used for WB of VDAC1 in GF-1 cells transfected with EYFP or EYFP-B2 and treated with and without NAC or Mdivi for 48 h (Fig. 6C).
WB Fish
Lee TY, Liu MS, Huang LJ, Lue SI. The Immediate Mitochondrial Stress Response in Coping with Systemic Exposure of Silver Nanoparticles in Rat Liver. Nanomedicine & Nanotechnology. 2014 Aug 27 (WB, Rat) WB Rat
Zhu Y, Liu Q, Liao M et al. Overexpression of LncRNA EPB41L4A-AS1 induces metabolic reprogramming in trophoblast cells and placenta tissue of miscarriage Molecular Therapy - Nucleic Acids Sep 1 2019 [PMID: 31671345] (KD, WB, Human) KD, WB Human
Liu KE, Frazier WA. Phosphorylation of the BNIP3 C-Terminus Inhibits Mitochondrial Damage and Cell Death without Blocking Autophagy PLoS ONE. 2015 Jun 24 [PMID: 26102349] (WB, Human) WB Human
Chen H, Dzitoyeva S, Manev H. Effect of valproic acid on mitochondrial epigenetics Eur J Pharmacol 2012 Jun 20 [PMID: 22728245] (WB, Mouse) WB Mouse

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Bioinformatics

Gene Symbol VDAC1
Entrez
Uniprot