VDAC1 Antibody

Images

 
Western Blot: VDAC1 Antibody [NB100-695] - The BNIP3 C-terminus can be phosphorylated. Subcellular localization of BNIP3 phosphomutants, showing Western blot of cytosolic and mitochondrial fractions. Image collected and ...read more
Western Blot: VDAC1 Antibody [NB100-695] - Lane 1: rat heart whole cell lysate. Lane 2: none. Load: 35 ug per lane. Primary antibody: VDAC/Porin antibody at 1:1,200 for overnight at 4C. Secondary antibody: IRDye800 (TM) ...read more
Western Blot: VDAC1 Antibody [NB100-695] - The BNIP3 C-terminus can be phosphorylated. Alkaline extraction of mitochondria-associated proteins, showing alkaline extract of the mitochondrial pellet and the ...read more
Western Blot of Rabbit anti-VDAC/Porin antibody. Marker: Opal Pre-stained ladder
Western Blot of Rabbit anti-VDAC/Porin antibody. Lane 1: MW ladder. Lane 2: Mouse Heart WCL. Load: 10 ug per lane. Primary antibody: VDAC/Porin antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, FiSpecies Glossary
Applications WB, Simple Western, ELISA, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

VDAC1 Antibody Summary

Description
The product was affinity purified from monospecific antiserum by immunoaffinity purification

Store this antibody at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Immunogen
VDAC1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 175-200 of Human VDAC1/Porin1. (Uniprot: P21796)
Localization
Mitochondria, cell membrane
Marker
Mitochondria Marker
Specificity
Anti-VDAC1 Antibody is directed against human VDAC1/Porin1 protein. A BLAST analysis was used to suggest that this antibody would react with VDAC1/Porin1 from a wide range or organisms, including avian, mammalian, aquatic and reptilian sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all forms of VDACs including VDAC1, VDAC2 (4 isoforms) and VDAC3 (2 isoforms). Such broad reactivity makes this antibody useful as an excellent loading control (mitochondrial).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VDAC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:15000-1:30000
  • Immunohistochemistry 1:10-1:500
  • Knockdown Validated
  • Simple Western
  • Western Blot 1:500-1:2000
Application Notes
This product has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~30-33 kDa in size corresponding to VDAC/Porin by western blotting in the appropriate cell lysate or extract.
Publications
Read Publications using
NB100-695 in the following applications:

Reactivity Notes

A BLAST analysis was used to suggest that this antibody would react with VDAC1/Porin1 from a wide range or organisms, including avian, mammalian, aquatic and reptilian sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all forms of VDACs including VDAC1, VDAC2 (4 isoforms) and VDAC3 (2 isoforms)
Mouse reactivity reported in scientific literature (PMID: 22728245). Rat reactivity reported in scientific literature (DOI: 10.4172/2157-7439.1000220).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for VDAC1 Antibody

  • hVDAC1
  • MGC111064
  • Outer mitochondrial membrane protein porin 1
  • Plasmalemmal porin
  • Porin 31HL
  • Porin 31HM
  • PORIN
  • PORIN-31-HL
  • VDAC
  • VDAC1
  • VDAC-1
  • voltage-dependent anion channel 1
  • voltage-dependent anion-selective channel protein 1

Background

VDAC1 is an outer membrane mitochondrial protein. The VDAC proteins are thought to form aqueous channels, or pores, through which adenine nucleotides cross the outer mitochondrial membrane. VDACs have been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT), and cyclophilin D (CypD), is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis. VDACs are highly expressed in heart, liver and skeletal muscle, where concentrations of mitochondria are at their highest.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-20849
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
NB100-56503
Species: Dr, Hu, Mu, Rb, Rt
Applications: Flow-CS, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-P, Simple Western, WB
NB100-56104
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, IP, WB
NBP1-28566
Species: Bv, Hu, Pm, Mu, Po, Rt
Applications: Func, IHC, IHC-Fr, IHC-P, WB
NBP1-80070
Species: Hu
Applications: IHC, IHC-P, WB
NB110-74751
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB100-56098
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
NBP2-92642
Species: Hu, Mu, Rt
Applications: ICC/IF, KO, WB
NBP1-62583
Species: Hu
Applications: WB
NB100-41398
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
NBP2-92677
Species: Hu, Mu, Rt
Applications: WB
NB100-74635
Species: Hu
Applications: IHC, IHC-P, IP, WB (-)
NBP2-30949
Species: Hu
Applications: IHC, IHC-P
NBP1-97512
Species: Mu
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP1-47974
Species: Ca, Hu, Pm, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-81293
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
AF816
Species: Hu
Applications: ICC, IHC, WB
AF860
Species: Hu, Mu
Applications: IP, Simple Western, WB
NBP1-32870
Species: Ch, Hu
Applications: IHC, IHC-P, IP, KD, WB
NB100-695
Species: Hu, Mu, Rt, Fi
Applications: WB, Simple Western, ELISA, IHC, KD

Publications for VDAC1 Antibody (NB100-695)(9)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Cynomolgus Monkey, Fish.

We have publications tested in 3 applications: KD, Simple Western, WB.


Filter By Application
KD
(1)
Simple Western
(1)
WB
(7)
All Applications
Filter By Species
Human
(3)
Mouse
(1)
Rat
(1)
Cynomolgus Monkey
(1)
Fish
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB100-695 Applications Species
Kam JH, Shinhmar H, Powner MB et al. Mitochondrial decline in the ageing old world primate retina: Little evidence for difference between the centre and periphery PloS one 2023-05-02 [PMID: 37130143] (WB, Cynomolgus Monkey)

Details:
0.05ug/ml
WB Cynomolgus Monkey
Arumugam S, Li B, Boodapati SLT et al. Mitochondrial DNA and the STING pathway are required for hepatic stellate cell activation Hepatology (Baltimore, Md.) 2023-04-05 [PMID: 37013923]
Moreau, M, Capallere, C, et al. Reconstruction of functional human epidermis equivalent containing 5%IPS-derived keratinocytes treated with mitochondrial stimulating plant extracts. Sci Rep [PMID: 35641783] (Simple Western, Human)

Details:
0.180555556
Simple Western Human
Go G, Yoon Ym, Yoon S Et Al. Melatonin Protects Chronic Kidney Disease Mesenchymal Stem/Stromal Cells against Accumulation of Methylglyoxal via Modulation of Hexokinase-2 Expression Biomolecules & therapeutics 2021-07-15 [PMID: 34261819] (WB) WB
Liu KE, Frazier WA. Phosphorylation of the BNIP3 C-Terminus Inhibits Mitochondrial Damage and Cell Death without Blocking Autophagy PLoS ONE. 2015-06-24 [PMID: 26102349] (WB, Human) WB Human
Su YC, Chiu HW, Hung JC, Hong JR. Beta-nodavirus B2 protein induces hydrogen peroxide production, leading to Drp1-recruited mitochondrial fragmentation and cell death via mitochondrial targeting. Apoptosis 2014-07-10 [PMID: 25008790] (WB, Fish)

Details:
DRP1 antibody used for WB at 1:500 dilution on mitochondrial fractions isolated from Grouper/Epinephelus coioides cell line GF-1 cells (a cell line susceptible to grouper nervous necrosis virus - GNNV) transfected with EYFP or EYFP-B2 and treated with and without NAC or Mdivi for 48 h (Fig 6C).
WB Fish
Chen H, Dzitoyeva S, Manev H. Effect of valproic acid on mitochondrial epigenetics Eur J Pharmacol 2012-06-20 [PMID: 22728245] (WB, Mouse) WB Mouse
Lee TY, Liu MS, Huang LJ, Lue SI. The Immediate Mitochondrial Stress Response in Coping with Systemic Exposure of Silver Nanoparticles in Rat Liver. Nanomedicine & Nanotechnology. 2014-08-27 (WB, Rat) WB Rat
Zhu Y, Liu Q, Liao M et al. Overexpression of LncRNA EPB41L4A-AS1 induces metabolic reprogramming in trophoblast cells and placenta tissue of miscarriage Molecular Therapy - Nucleic Acids 2019-09-01 [PMID: 31671345] (KD, WB, Human) KD, WB Human

Reviews for VDAC1 Antibody (NB100-695) (0)

There are no reviews for VDAC1 Antibody (NB100-695). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for VDAC1 Antibody (NB100-695) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional VDAC1 Products

Diseases for VDAC1 Antibody (NB100-695)

Discover more about diseases related to VDAC1 Antibody (NB100-695).
 

Pathways for VDAC1 Antibody (NB100-695)

View related products by pathway.

PTMs for VDAC1 Antibody (NB100-695)

Learn more about PTMs related to VDAC1 Antibody (NB100-695).
 

Research Areas for VDAC1 Antibody (NB100-695)

Find related products by research area.

Blogs on VDAC1

There are no specific blogs for VDAC1, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our VDAC1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol VDAC1
Entrez
Uniprot