VDAC1 Antibody


Western Blot: VDAC1 Antibody [NB100-695] - The BNIP3 C-terminus can be phosphorylated. Subcellular localization of BNIP3 phosphomutants, showing Western blot of cytosolic and mitochondrial fractions. Image collected and ...read more
Western Blot: VDAC1 Antibody [NB100-695] - Lane 1: rat heart whole cell lysate. Lane 2: none. Load: 35 ug per lane. Primary antibody: VDAC/Porin antibody at 1:1,200 for overnight at 4C. Secondary antibody: IRDye800 (TM) ...read more
Western Blot: VDAC1 Antibody [NB100-695] - The BNIP3 C-terminus can be phosphorylated. Alkaline extraction of mitochondria-associated proteins, showing alkaline extract of the mitochondrial pellet and the ...read more

Product Details

Reactivity Hu, Mu, Rt, Fi, Po, Bv, RbSpecies Glossary
Applications WB, ELISA, IHC, KD

Order Details

VDAC1 Antibody Summary

VDAC1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 175-200 of Human VDAC1/Porin1. (Uniprot: P21796)
Mitochondria, cell membrane
Mitochondria Marker
Cross reactivity will occur with all forms of VDACs including VDAC1, VDAC2 (4 isoforms) and VDAC3 (2 isoforms). Such broad reactivity makes this antibody useful as an excellent loading control (mitochondrial).
Predicted Species
Porcine (100%), Bovine (100%), Rabbit (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • ELISA 1:15000-1:30000
  • Immunohistochemistry 1:10-1:500
  • Knockdown Validated
  • Western Blot 1:500-1:2000
Application Notes
Specific conditions for reactivity should be optimized by the end user. Expect a band at ~30-33 kDa in size corresponding to VDAC1 by western blotting in the appropriate cell lysate or extract.
Theoretical MW
30.77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NB100-695 in the following applications:

  • KD
    1 publication
  • WB
    6 publications

Reactivity Notes

A BLAST analysis was used to suggest that this antibody would react with VDAC1/Porin1 from a wide range or organisms, including avian, mammalian, aquatic and reptilian sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all forms of VDACs including VDAC1, VDAC2 (4 isoforms) and VDAC3 (2 isoforms)
Mouse reactivity reported in scientific literature (PMID: 22728245). Rat reactivity reported in scientific literature (DOI: 10.4172/2157-7439.1000220).

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% Sodium Azide
Immunogen affinity purified


The product was affinity purified from monospecific antiserum by immunoaffinity purification

Store this antibody at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for VDAC1 Antibody

  • hVDAC1
  • MGC111064
  • Outer mitochondrial membrane protein porin 1
  • Plasmalemmal porin
  • Porin 31HL
  • Porin 31HM
  • PORIN-31-HL
  • VDAC
  • VDAC1
  • VDAC-1
  • voltage-dependent anion channel 1
  • voltage-dependent anion-selective channel protein 1


VDAC1 is an outer membrane mitochondrial protein. The VDAC proteins are thought to form aqueous channels, or pores, through which adenine nucleotides cross the outer mitochondrial membrane. VDACs have been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT), and cyclophilin D (CypD), is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis. VDACs are highly expressed in heart, liver and skeletal muscle, where concentrations of mitochondria are at their highest.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
Species: Dr, Hu, Mu, Rb, Rt
Applications: Flow-CS, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-P, Simple Western, WB
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, IP, WB
Species: Bv, Hu, Pm, Mu, Po, Rt
Applications: Func, IHC, IHC-Fr, IHC-P, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, KO, WB
Species: Hu
Applications: ICC/IF, WB
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
Species: Hu, Mu, Rt
Applications: WB
Species: Hu
Applications: IHC, IHC-P, IP, WB (-)
Species: Hu
Applications: IHC, IHC-P
Species: Mu
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Species: Ca, Hu, Pm, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
Species: Hu, Mu
Applications: IP, Simple Western, WB
Species: Ch, Hu
Applications: IHC, IHC-P, IP, KD, WB
Species: Hu, Mu, Rt, Fi, Po, Bv, Rb
Applications: WB, ELISA, IHC, KD

Publications for VDAC1 Antibody (NB100-695)(6)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Fish.

We have publications tested in 2 applications: KD, WB.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 6 of 6.
Publications using NB100-695 Applications Species
Liu KE, Frazier WA. Phosphorylation of the BNIP3 C-Terminus Inhibits Mitochondrial Damage and Cell Death without Blocking Autophagy PLoS ONE. 2015 Jun 24 [PMID: 26102349] (WB, Human) WB Human
Su YC, Chiu HW, Hung JC, Hong JR. Beta-nodavirus B2 protein induces hydrogen peroxide production, leading to Drp1-recruited mitochondrial fragmentation and cell death via mitochondrial targeting. Apoptosis 2014 Jul 10 [PMID: 25008790] (WB, Fish)

VDAC1 antibody used in WB application at 1:500 dilution as mitochondrial marker for fractions isolated from Grouper/Epinephelus coioides cell line GF-1 (a cell line susceptible to grouper nervous necrosis virus - GNNV) transfected with Flag, betanodavirus B2 gene, and B2-delta as well as MCF-7 cells (Fig 1C). Antibody also used for WB of VDAC1 in GF-1 cells transfected with EYFP or EYFP-B2 and treated with and without NAC or Mdivi for 48 h (Fig. 6C).
WB Fish
Chen H, Dzitoyeva S, Manev H. Effect of valproic acid on mitochondrial epigenetics Eur J Pharmacol 2012 Jun 20 [PMID: 22728245] (WB, Mouse) WB Mouse
Lee TY, Liu MS, Huang LJ, Lue SI. The Immediate Mitochondrial Stress Response in Coping with Systemic Exposure of Silver Nanoparticles in Rat Liver. Nanomedicine & Nanotechnology. 2014 Aug 27 (WB, Rat) WB Rat
Go G, Yoon Ym, Yoon S Et Al. Melatonin Protects Chronic Kidney Disease Mesenchymal Stem/Stromal Cells against Accumulation of Methylglyoxal via Modulation of Hexokinase-2 Expression Biomolecules & therapeutics Jul 15 2021 [PMID: 34261819] (WB) WB
Zhu Y, Liu Q, Liao M et al. Overexpression of LncRNA EPB41L4A-AS1 induces metabolic reprogramming in trophoblast cells and placenta tissue of miscarriage Molecular Therapy - Nucleic Acids Sep 1 2019 [PMID: 31671345] (KD, WB, Human) KD, WB Human

Reviews for VDAC1 Antibody (NB100-695) (0)

There are no reviews for VDAC1 Antibody (NB100-695). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for VDAC1 Antibody (NB100-695) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Additional VDAC1 Products

Bioinformatics Tool for VDAC1 Antibody (NB100-695)

Discover related pathways, diseases and genes to VDAC1 Antibody (NB100-695). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for VDAC1 Antibody (NB100-695)

Discover more about diseases related to VDAC1 Antibody (NB100-695).

Pathways for VDAC1 Antibody (NB100-695)

View related products by pathway.

PTMs for VDAC1 Antibody (NB100-695)

Learn more about PTMs related to VDAC1 Antibody (NB100-695).

Research Areas for VDAC1 Antibody (NB100-695)

Find related products by research area.

Blogs on VDAC1

There are no specific blogs for VDAC1, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our VDAC1 Antibody and receive a gift card or discount.


Gene Symbol VDAC1