Western Blot: VDAC1 Antibody [NB100-695] - Lane 1: rat heart whole cell lysate. Lane 2: none. Load: 35 ug per lane. Primary antibody: VDAC/Porin antibody at 1:1,200 for overnight at 4C. Secondary antibody: IRDye800 (TM) ...read more
This affinity purified antibody has been tested for use in ELISA and western blot. Expect a band at ~30-33 kDa in size corresponding - VDAC/Porin by western blotting in the appropriate cell lysate or extract.
30.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
A BLAST analysis was used to suggest that this antibody would react with VDAC1 from avian, mammalian, aquatic and reptilian sources based on 100% homology for the immunogen sequence.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% Sodium Azide
Immunogen affinity purified
Alternate Names for VDAC1 Antibody
Outer mitochondrial membrane protein porin 1
voltage-dependent anion channel 1
voltage-dependent anion-selective channel protein 1
VDAC1 is an outer membrane mitochondrial protein. The VDAC proteins are thought to form aqueous channels, or pores, through which adenine nucleotides cross the outer mitochondrial membrane. VDACs have been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT), and cyclophilin D (CypD), is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis. VDACs are highly expressed in heart, liver and skeletal muscle, where concentrations of mitochondria are at their highest.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Lee TY, Liu MS, Huang LJ, Lue SI. The Immediate Mitochondrial Stress Response in Coping with Systemic Exposure of Silver Nanoparticles in Rat Liver. Nanomedicine & Nanotechnology. 2014 Aug 27 (WB, Rat)
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