Rabbit VCAM-1/CD106 ELISA Kit (Colorimetric)

ELISA: Rabbit VCAM-1/CD106 ELISA Kit (Colorimetric) [NBP3-08192] - Samples were spiked with high concentrations of Rabbit VCAM-1/CD106 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rabbit VCAM-1/CD106 ELISA Kit (Colorimetric) [NBP3-08192] - Standard Curve Reference

• Reactivity: Rb
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell supernatant
• Standard Curve Range: 62.5 - 4000 pg/mL
• Sensitivity: 37.5 pg/mL

Rabbit VCAM-1/CD106 ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 62.5 - 4000 pg/mL
• Sensitivity: 37.5 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: %CV < 5.75
• Intra-Assay: %CV < 6.71
• Spike Recovery: 91 - 108%
• Kit Type: ELISA Kit (Colorimetric)
• Gene: VCAM1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rabbit VCAM-1/CD106 ELISA Kit (Colorimetric)

• CD106 antigen
• CD106
• DKFZp779G2333
• INCAM-100
• L1CAM
• MGC99561
• vascular cell adhesion molecule 1
• vascular cell adhesion protein 1
• V-CAM 1
• VCAM1
• VCAM-1

Background

CD106 is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 and INCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 ( alpha4/ beta1 integrin) and LPAM-1 ( alpha4/ beta7 integrin). The 429 antibody has been reported to partially block VCAM-1-mediated binding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for VCAM-1/CD106 ELISA Kit (NBP3-08192). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: VCAM1