| Reactivity | MuSpecies Glossary |
| Applications | Flow, IHC, IHC-Fr, ICC/IF |
| Clone | MVCAM A (429) |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | DyLight 755 |
| Immunogen | Stromal cell line PA6 |
| Specificity | Recognizes murine vascular adhesion molecule 1 (VCAM-1), a cell surface glycoprotein that is also known as CD106. CD106 is expressed predominantly on endothelial cells and expression is up-regulated during inflammation. The ligand for CD106 is the alpha 4 subunit (CD49d) of the integrin VLA-4 (CD49d/CD29). Clone MVCAM.A(429) is reported to partially block VCAM-1 mediated functions. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rat |
| Gene | VCAM1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
![VCAM-1/CD106 Antibody (MVCAM A (429)) [DyLight 755]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "VCAM-1/CD106 Antibody (MVCAM A (429)) [DyLight 755]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-50620AF350 | |
| Alexa Fluor 405 | NBP2-50620AF405 | |
| Alexa Fluor 488 | NBP2-50620AF488 | |
| Alexa Fluor 532 | NBP2-50620AF532 | |
| Alexa Fluor 594 | NBP2-50620AF594 | |
| Alexa Fluor 647 | NBP2-50620AF647 | |
| Alexa Fluor 700 | NBP2-50620AF700 | |
| Alexa Fluor 750 | NBP2-50620AF750 | |
| Allophycocyanin | NBP2-50620APC | |
| Allophycocyanin/Cy7 | NBP2-50620APCCY7 | |
| Biotin | NBP2-50620B | |
| DyLight 350 | NBP2-50620UV | |
| DyLight 405 | NBP2-50620V | |
| DyLight 488 | NBP2-50620G | |
| DyLight 550 | NBP2-50620R | |
| DyLight 594 | NBP2-50620DL594 | |
| DyLight 650 | NBP2-50620C | |
| DyLight 680 | NBP2-50620FR | |
| DyLight 755 | NBP2-50620IR | |
| FITC | NBP2-50620F | |
| HRP | NBP2-50620H | |
| Janelia Fluor 549 | NBP2-50620JF549 | |
| Janelia Fluor 646 | NBP2-50620JF646 | |
| PE | NBP2-50620PE | |
| PE/Atto594 | NBP2-50620PEATT594 | |
| PE/Cy5.5 | NBP2-50620PECY55 | |
| PE/Cy7 | NBP2-50620PECY7 | |
| PerCP | NBP2-50620PCP |
Diseases for VCAM-1/CD106 Antibody (NBP2-50620IR)Discover more about diseases related to VCAM-1/CD106 Antibody (NBP2-50620IR).
| Pathways for VCAM-1/CD106 Antibody (NBP2-50620IR)View related products by pathway.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | VCAM1 |
![Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/images2/HLA DQ Antibody (SPV-L3)-Flow Cytometry-NBP2-45041-img0001.jpg)
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![Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0013.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of paraffin-embedded human lung cancer tissues using anti-CD44 mAb (green), showing membrane localization. DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/images2/CD44-Antibody-(8E2F3)-Immunocytochemistry-Immunofluorescence-NBP1-47386-img0011.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of FFPE human breast carcinoma tissues using CD44 antibody (8E2F3). The signal was developed using DAB based detection and the sections were processed for counterstaining with hematoxylin. The antibody generated mainly a membrane staining representative of CD44 protein.](http://images.novusbio.com/images2/CD44-Antibody-(8E2F3)-Immunohistochemistry-Paraffin-NBP1-47386-img0014.jpg)
![Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of HeLa cells using anti-CD44 mAb (right) and negative control (left).](http://images.novusbio.com/images2/CD44-Antibody-(8E2F3)-Flow-Cytometry-NBP1-47386-img0008.jpg)
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![Flow Cytometry: Integrin alpha L/CD11a Antibody (MEM-25) [NB500-328] - Analysis using the PE conjugate of NB500-328. Surface staining of human peripheral blood cells with anti-human CD11a (MEM-25) PE.](http://images.novusbio.com/images2/Integrin-alpha-L-CD11a-Antibody-(MEM-25)-Flow-Cytometry-NB500-328-img0002.jpg)
![Flow Cytometry: Integrin alpha L/CD11a Antibody (MEM-25) [NB500-328] - Staining pattern of human peripheral whole blood stained using anti-human CD11a (MEM-25) purified antibody (concentration in sample 1 ug/ml) GAM APC.](http://images.novusbio.com/images2/Integrin-alpha-L-CD11a-Antibody-(MEM-25)-Flow-Cytometry-NB500-328-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - Integrin beta 1 (P4C10) antibody was tested in -20 degree MeOH fixed HeLa cells at a 1:200 dilution against DyLight 488 (green). Actin and nuclei were counterstained against Phalloidin 568 (red) and DAPI (blue), respectively.](http://images.novusbio.com/images2/Integrin-beta-1-CD29-Antibody-(P4C10)-Immunocytochemistry-Immunofluorescence-NBP2-36561-img0001.jpg)
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - An intracellular stain was performed on A431 cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).](http://images.novusbio.com/images2/Integrin-beta-1-CD29-Antibody-(P4C10)-Flow-Cytometry-NBP2-36561-img0005.jpg)
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561]](http://images.novusbio.com/images2/Integrin-beta-1-CD29-Antibody-(P4C10)-Flow-Cytometry-NBP2-36561-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 antibody was tested in WEHI-3 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/images2/CD34 Antibody (MEC 14.7)-Immunocytochemistry-Immunofluorescence-NB600-1071-img0003.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of mouse small intestine using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using HRP-conjugated anti-rat secondary with DAB reagent which followed counterstaining of nuclei using hematoxylin. This antibody specifically labelled the endothelial cells in blood vessels located primarily in the sub-mucosa, and of that of the mucosa muscularis and the mucosal lacteal.](http://images.novusbio.com/images2/CD34-Antibody-(MEC-14.7)-Immunohistochemistry-Paraffin-NB600-1071-img0014.jpg)
![Flow Cytometry: CD34 Antibody (MEC 14.7) [NB600-1071] - An intracellular stain was performed on Raw264.7 cells with CD34 Antibody [MEC 14.7] NB600-1071 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 488 (SA5-10018, Thermo Fisher).](http://images.novusbio.com/images2/CD34-Antibody-(MEC-14.7)-Flow-Cytometry-NB600-1071-img0015.jpg)
![VCAM-1/CD106 [HRP]](http://images.novusbio.com/images2/VCAM-1_DVC00_ELISA_206.jpg "VCAM-1/CD106 [HRP]")