VCAM-1/CD106 Antibody (IE10) [Allophycocyanin]

HuT 78 human cutaneous T cell lymphoma cell line was stained with Mouse Anti-Human VCAM-1/CD106 APC-conjugated Monoclonal Antibody (Catalog # FAB5649A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: IE10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

VCAM-1/CD106 Antibody (IE10) [Allophycocyanin] Summary

• Immunogen: Activated HUVEC human umbilical vein endothelial cells
• Specificity: The antibody was screened using COS cells transfected with cDNAs for E-Selectin, VCAM-1, ICAM-1 and PECAM-1 and was shown to be specific for human VCAM-1/CD106.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: VCAM1
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VCAM-1/CD106 Antibody (IE10) [Allophycocyanin]

• CD106 antigen
• CD106
• DKFZp779G2333
• INCAM-100
• L1CAM
• MGC99561
• vascular cell adhesion molecule 1
• vascular cell adhesion protein 1
• V-CAM 1
• VCAM1
• VCAM-1

Background

Vascular cell adhesion molecule 1 (VCAM-1) is expressed on the surface of activated endothelial cells and macrophages. It binds to leukocyte integrins VLA-4 and alpha 4 beta 7 to promote leukocyte adhesion at sites of inflamed vasculature.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VCAM-1/CD106 Antibody (FAB5649A)(3)

Publications using FAB5649A

• H Watanabe, S Goto, R Kato, S Komiyama, Y Nagaoka, T Kazama, C Yamamoto, Y Li, N Konuma, K Hagikura, T Matsumoto The neovascularization effect of dedifferentiated fat cells Sci Rep, 2020-06-08;10(1):9211. 2020-06-08 [PMID: 32514018] (Flow Cytometry, Mouse)
• N Erbeldinge, F Rapp, S Ktitareva, P Wendel, AS Bothe, T Dettmering, M Durante, T Friedrich, B Bertulat, S Meyer, MC Cardoso, S Hehlgans, F Rödel, C Fournier Measuring Leukocyte Adhesion to (Primary) Endothelial Cells after Photon and Charged Particle Exposure with a Dedicated Laminar Flow Chamber Front Immunol, 2017-06-01;8(0):627. 2017-06-01 [PMID: 28620384] (Flow Cytometry, Human)
• KE Happonen, S Tran, M Mörgelin, R Prince, S Calzavarin, A Angelillo-, B Dahlbäck The Gas6-Axl Protein Interaction Mediates Endothelial Uptake of Platelet Microparticles J. Biol. Chem., 2016-03-22;291(20):10586-601. 2016-03-22 [PMID: 27006397] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: VCAM1